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Action of Aqueous Extracts of Phyllanthus niruri L. (Euphorbiaceae) leaves on Meristematic Root Cells of Allium cepa L. Anais da ABC (AABC)
NEVES,ERASMOVLANE S.B.; FERREIRA,PAULO MICHEL PINHEIRO; LIMA,LEONARDO H.G.M.; PERON,ANA PAULA.
This study aimed to evaluate the effects of aqueous extracts of dried Phyllanthus niruri L. (stonebreaker) leaves on Allium cepa L. root meristem cells at four concentrations, 0.02 (usual concentration), 0.04, 0.06 and 0.08mg/mL and exposure times of 24 and 48 hours. For each concentration we used a group of five onion bulbs that were first embedded in distilled water and then transferred to their respective concentrations. The radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were prepared by the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5000 for each control and exposure time. The calculated mitotic indices were subjected to the Chi-squared statistical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plant; Stonebreaker; Cell division; Cellular aberrations; Allium cepa.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301131
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Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L. Biological Sciences
Santana, Gleuvânia Marques; Sousa, Josefa Janaína do Anjos; Peron, Ana Paula.
This study aimed to evaluate the toxicity of Ponceau 4R food dye on the cell cycle in root meristematic cells of Allium cepa L. at three concentrations: 0.25, 0.50 and 0.75 g L-1, at exposure times of 24 and 48 hours. For each concentration, we used a set of five onion bulbs that were first rooted in distilled water and then transferred to their respective concentrations. Radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were mounted with the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5,000 cells for each control and exposure time. The calculated mitotic indices were subjected to the Chi-square test (p < 0.05). From the results, we observed that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tecnolologia de alimentos citogenética azo dye; Cell division; Cellular aberrations; Cytotoxicity.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23119
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Cytotoxic and genotoxic potential of powdered juices Ciênc. Tecnol. Aliment.
BEZERRA,Marielly de Sousa; MALAQUIAS,Geiz de Sousa; CASTRO E SOUSA,João Marcelo de; PERON,Ana Paula.
Abstract Powdered juices are widely consumed by the population especially because of their convenient preparation, availability in various fruit flavors and low cost when compared to other industrialized beverages. They have complex formulation, consisting of several classes of food additives. However, there are no scientific studies on the toxicity of these foods. Thus, this study evaluated the toxicity at the cellular level of industrialized powdered juices of orange and guava flavors of three different food companies. This analysis was made using root meristem cells of Allium cepa L., at the exposure times of 24 and 48 hours, and two concentrations, 30 g/1000 mL, considered ideal for consumption according to the label of the products, and 30 g/500 mL....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Industrialized juice; Toxicity at the cellular level; Mitotic index; Cellular aberrations; Allium cepa.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000100049
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