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| Aton, Emilie; Renault, Tristan; Gagnaire, Beatrice; Thomas Guyon, Hélène; Cognard, C; Imbert, N. |
| Bivalve haemocytes are essential in defence mechanisms including phagocytosis. They also produce molecules including hydrolytic enzymes and antimicrobial peptides that contribute to pathogen destruction. Although haemocyte activities have been extensively studied, relatively little is known about the intracellular signalling pathways that are evoked during haemocyte activation and especially the role of calcium. Flow cytometry has been used for the first time to define the effect of cell incubation in haemolymph and artificial sea water (ASW) on Pacific oyster, Crassostrea gigas, haemocytes. Cell viability, enzymatic activities (esterases and amino peptidases), phagocytosis and granulocyte percentage were analysed. Viability and some activities were... |
| Tipo: Text |
Palavras-chave: Cellular activities; Fluo 3/AM; Calcium; Flow cytometry; Haemocytes; Crassostrea gigas. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-935.pdf |
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| Gagnaire, Beatrice; Thomas Guyon, H; Burgeot, Thierry; Renault, Tristan. |
| The shellfish industry is an important economic activity in France, occurring mostly in estuarine zones subject to pollution due to anthropogenic activities. The harmful effects of pollutants on species inhabiting these estuarine zones are not well known. Among marine species, bivalve mollusks---particularly Pacific oyster, Crassostrea gigas-may serve a model of interest. The species is sedentary and filter-feeding, which favors bioaccumulation of pollutants in their tissues. Oysters may be suitable for studies on disturbance by pollutants of physiological activities, among which defense mechanisms are poorly documented in bivalves. In this study, effects of pollutants on hemocyte functions were monitored in Pacific oyster, C. gigas. Hemocytes were exposed... |
| Tipo: Text |
Palavras-chave: Toxicity; Pesticides; PCBs; PAHs; Crassostrea gigas; Pacific oyster; Hemocytes; Flow cytometry; Cellular activities. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1695.pdf |
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