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Registros recuperados: 9
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Chitinase from Enterobacter sp. NRG4: Its purification, characterization and reaction pattern Electron. J. Biotechnol.
Dahiya,Neetu; Tewari,Rupinder; Tiwari,Ram P; Singh Hoondal,Gurinder.
Enterobacter sp. NRG4 was shown to excrete chitinase into the culture supernatant when cultivated in medium containing chitin. A 60 kDa extracellular chitinase was purified to homogeneity and characterized. The enzyme hydrolyzed swollen chitin, colloidal chitin, regenerated chitin and glycol chitin but did not hydrolyze chitosan. The chitinase exhibited Km and Vmax values of 1.43 mg ml-1 and 83.33 µM µg-1 h-1 for swollen chitin, 1.41 mg ml-1 and 74.07 µM µg-1 h-1 for colloidal chitin, 1.8 mg ml-1 and 40 µM µg-1 h-1 for regenerated chitin and 2.0 mg ml-1 and 33.33 µM µg-1 h-1 for glycol chitin, respectively. The optimal temperature and pH for activity were 45ºC and pH 5.5, respectively. Mg2+, K+ and Ca2+ stimulated chitinase activity by 13, 16 and 18%,...
Tipo: Journal article Palavras-chave: Chemical modification; Chitinase; Enterobacter sp. NRG4; Purification; Substrate binding.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200003
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Design Polysaccharides of Marine Origin: Chemical Modifications to Reach Advanced Versatile Compounds ArchiMer
Chopin, Nathalie; Guillory, Xavier; Weiss, Pierre; Le Bideau, Jean; Colliec-jouault, Sylvia.
Polysaccharides are among the most abundant macromolecules on Earth. These polymers are easily obtained from various marine resources such as algae, microorganisms and crustacean shells. The structure of these natural carbohydrates is innovative and quite complex. Marine biopolymers represent key scaffolds toward large challenging fields, such as biomedical applications (glycosaminoglycans, regenerative medicine and drug delivery) and tailored biomaterials. Chemical modifications can be applied to modify their final properties in a specific purpose. New functional glycans are achievable and represent a real potential with their intrinsic biocompatibility and biodegradability. Hydroxyl groups are ubiquitous in polysaccharides structure and involved in most...
Tipo: Text Palavras-chave: Alginates; Carrageenans; Chemical modification; Chitin; Chitosan; Grafting; Marine polysaccharide; Polymer.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00199/31027/29902.pdf
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Effect of treatment with alkaline hydrogen peroxide associated with extrusion on color and hydration properties of oat hulls BABT
Galdeano,Melicia Cintia; Grossmann,Maria Victória Eiras.
An incomplete factorial design with three independent variables at three levels of variation was used to evaluate the effect of the treatment with alkaline hydrogen peroxide concomitant with extrusion on some properties of oat hulls. The independent variables were hydrogen peroxide level, feed moisture and extrusion temperature. The dependent variables were water retention capacity (WRC), swollen volume (SV) and color. The analysis of variance showed that temperature was the most important variable for the hydration properties. The highest values of WRC and SV were obtained when oat hulls were processed in the condition of 7% hydrogen peroxide, 32% feed moisture and 90ºC extrusion temperature. Modified hulls had a WRC 70% higher and SV 55% higher than the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dietary fiber; Oat hulls; Chemical modification; Functional properties.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100010
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Functional characterization of acetylated Brazil nut (Bertholletia excelsa HBK) kernel globulin Ciênc. Tecnol. Aliment.
Ramos,Cíntia Maria Pinto; Bora,Pushkar Singh.
Defatted Brazil nut kernel flour, a rich source of high quality proteins, is presently being utilized in the formulation of animal feeds. One of the possible ways to improve its utilization for human consumption is through improvement in its functional properties. In the present study, changes in some of the functional properties of Brazil nut kernel globulin were evaluated after acetylation at 58.6, 66.2 and 75.3% levels. The solubility of acetylated globulin was improved above pH 6.0 but was reduced in the pH range of 3.0-4.0. Water and oil absorption capacity, as well as the viscosity increased with increase in the level of acetylation. Level of modification also influenced the emulsifying capacity: decreased at pH 3.0, but increased at pH 7.0 and 9.0....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazil nut kernel globulin; Chemical modification; Acetylation; And functional properties.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612004000100024
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Identification of functionally important acidic residues in transducin by group-specific labeling Biol. Res.
KOSOY,ANA; MÖLLER,CAROLINA; PERDOMO,DEISY.
Transducin (T), a GTP-binding protein involved in phototransduction of rod photoreceptor cells, is a heterotrimer arranged as two units, the a-subunit (Ta) and the bg-complex (Tbg). The role of the carboxyl groups in T was evaluated by labeling with N, N'-dicyclohexylcarbodiimide (DCCD) and 1-ethyl 3-(3-dimethylaminopropyl) carbodiimide (EDC). Only a minor effect on the binding of b, g-imido guanosine 5'-triphosphate (GMPpNp) to T was observed in the presence of the hydrophobic carbodiimide, DCCD. Similarly, the GMPpNp binding activity of the reconstituted holoenzyme was not significantly affected when Ta was combined with DCCD-treated Tbg. However, the binding of guanine nucleotides to the reconstituted T was ~50% inhibited when DCCD-labeled Ta was...
Tipo: Journal article Palavras-chave: Chemical modification; G-protein-coupled signaling; Group-specific labeling; Transducin; Visual process.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300010
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Important amino acid residues of potato plant uncoupling protein (StUCP) BJMBR
Jezek,P.; Costa,A.D.T.; Vercesi,A.E..
Chemical modifications were used to identify some of the functionally important amino acid residues of the potato plant uncoupling protein (StUCP). The proton-dependent swelling of potato mitochondria in K+-acetate in the presence of linoleic acid and valinomycin was inhibited by mersalyl (Ki = 5 µM) and other hydrophilic SH reagents such as Thiolyte MB, iodoacetate and 5,5'-dithio-bis-(2-nitrobenzoate), but not by hydrophobic N-ethylmaleimide. This pattern of inhibition by SH reagents was similar to that of brown adipose tissue uncoupling protein (UCP1). As with UCP1, the arginine reagent 2,3-butadione, but not N-ethylmaleimide or other hydrophobic SH reagents, prevented the inhibition of StUCP-mediated transport by ATP in isolated potato mitochondria or...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant mitochondria; Uncoupling protein; Chemical modification; Mitochondrial swelling; Reconstitution.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200003
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Marine Polysaccharides: A Source of Bioactive Molecules for Cell Therapy and Tissue Engineering ArchiMer
Senni, Karim; Pereira, Jessica; Gueniche, Farida; Delbarre-ladrat, Christine; Sinquin, Corinne; Ratiskol, Jacqueline; Godeau, Gaston; Fischer, Anne-marie; Helley, Dominique; Colliec-jouault, Sylvia.
The therapeutic potential of natural bioactive compounds such as polysaccharides, especially glycosaminoglycans, is now well documented, and this activity combined with natural biodiversity will allow the development of a new generation of therapeutics. Advances in our understanding of the biosynthesis, structure and function of complex glycans from mammalian origin have shown the crucial role of this class of molecules to modulate disease processes and the importance of a deeper knowledge of structure-activity relationships. Marine environment offers a tremendous biodiversity and original polysaccharides have been discovered presenting a great chemical diversity that is largely species specific. The study of the biological properties of the...
Tipo: Text Palavras-chave: Marine bacteria; Marine algae; Exopolysaccharides; Sulfated polysaccharides; Structure; Chemical modification; Biological activity; Blue biotechnology; Cell therapy; Tissue engineering.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00048/15894/13316.pdf
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Modificação química de goma de cajueiro: novas características e potencialidades de aplicações. Infoteca-e
FURTADO, R. F.; RIBEIRO, F. W. M.; MENDES, L. G.; MARIANO, A. C. M.; ALVES, C. R.; BASTOS, M. do S. R.; COSTA, J. M. C. da.
2013
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Goma de cajueiro; Modificação química; Trimetafosfato de sódio; Cashew gum; Chemical modification; Sodium trimetaphosphate.
Ano: 2013 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/982098
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Terpene Esters from Natural Products: Synthesis and Evaluation of Cytotoxic Activity Anais da ABC (AABC)
VICTOR,MAURICIO M.; DAVID,JORGE M.; SAKUKUMA,MARIA C.K.; COSTA-LOTUFO,LETÍCIA V.; MOURA,ANDREA F.; ARAÚJO,ANA J..
ABSTRACT Natural steroids and triterpenes such as b-sitosterol, stigmasterol, lupeol, ursolic and betulinic acids were transformed into its hexanoic and oleic esters, to evaluate the influence of chemical modification towards the cytotoxic activities against tumor cells. The derivatives were evaluated against five tumor cell lines [OVCAR-8 (ovarian carcinoma); SF-295 (glioblastoma); HCT-116 (colon adenocarcinoma); HL-60 (leukemia); and PC-3 (prostate carcinoma)] and the results showed only betulinic acid hexyl ester exhibits cytotoxic potential activity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antitumor activity; Chemical modification; Derivative synthesis; Natural products esters.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401369
Registros recuperados: 9
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