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A VP3/VP1 gene polymerase chain reaction assay for detection of chicken anemia virus in broiler samples Arq. Bras. Med. Vet. Zootec.
Nogueira,E.O.; Brentano,L.; Ferreira,A.J.P..
A PCR assay was designed for amplification of the highly conserved VP3 gene and a 5' region of the VP1 gene, for the diagnosis of CAV in organ samples of broiler flocks suspected of chicken infectious anemia. A comparison of the VP3/VP1 PCR with in vivo virus isolation revealed 100% agreement of the results, with 13 positive and 3 negative samples in both assays, indicating that the VP3/VP1 PCR is a specific diagnostic method. Tissues from additional 24 broiler chicken flocks, with CAV-like lesions and clinical history were then tested only by the VP3/VP1 PCR and a reference PCR with published primers for the VP1 gene. Nineteen samples resulted positive and one negative in both PCR, while another 4 samples were positive only in the VP3/VP1 PCR. These...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken; Chicken anemia virus; CAV; PCR; Diagnostic test.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000800001
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Avaliação da viabilidade e patogenicidade de uma amostra de CAV mantida a -80°c por longo período. Repositório Alice
TREVISOL, I. M..
bitstream/item/218989/1/final9536.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: CAV; Vírus da anemia das aves; Ultracongelamento; Micro-organismo; Microrganismo; Armazenamento; Criopreservação; Pesquisa; Chicken anemia virus.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127886
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Diverse uses of feathers with emphasis on diagnosis of avian viral infections and vaccine virus monitoring Rev. Bras. Ciênc. Avic.
Davidson,I.
The large amounts of feathers produced by the poultry industry, that is considered as a waste was explored for possible uses in various industries, such as meals for animals, biofuels, biodegradable plastic materials, combating water pollution and more. That review mentions these uses, but concentrate on the utilization of feathers for the diagnosis of viral infections and for monitoring vaccine viruses in chickens after vaccination. The viral diseases in which diagnosis using nucleic acids extracted from the feather shafts was described are, Marek's disease virus, circoviruses, chicken anemia virus, fowlpox virus, avian retroviruses, avian influenza virus and infectious laryngotracheitis virus. In two cases, of Marek's disease virus and of infectious...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avian influenza virus; Avian retroviruses; Infectious laryngotracheitis virus; Circoviruses; Chicken; Chicken anemia virus; Diagnosis of avian viral diseases; Environmental monitoring; Feathers; Fowlpox virus; Marek's disease virus; Malformation of chick feathering; Stability of DNA viruses; Usage of feathers.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000300001
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Experimental Coinfection of Chicken Anemia Virus and Mycoplasma gallisepticum Vaccine Strains in Broiler Chicks Rev. Bras. Ciênc. Avic.
Prezotto,CF; Marin,SY; Araújo,TS; Barbosa,FO; Barrios,PR; Gomes,AM; Peconick,AP; Resende,M; Sousa,RV; Martins,NRS.
ABSTRACT This study aimed at determining the clinical and pathological effects of the coinfection of young SPF chickens with chicken anemia virus (CAV) and Mycoplasma gallisepticum (MG) vaccine strains. The clinical signs, gross and microscopic lesions were determined after the experimental coinfection broilers with a CAV genotype 1 vaccine strain given intraperitoneally on the first day of age and a MG F-strain vaccine given intranasally on the 8th day of age. The experimental groups included the negative control (group 1), a group infected with the MG F-strain vaccine (group 2), and a group coinfected with CAV and MG vaccines (group 3). Chicks were examined clinically and post mortem at 23 days of age, and gross and microscopic lesions of the trachea,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken; Chicken anemia virus; CAV; Mycoplasma gallisepticum; Coinfection.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000300475
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Improving and assessing viral vectors for recombinant protein production in plants. Repositório Alice
LACORTE, C..
Chapter 1 - Introduction; Chapter 2 - PVX and TMV -based viral vectors compatible with the GatewayTM cloning technology; Chapter 3 - Expression and localization of Chicken anemia virus (CA V) proteins in plant cells; Chapter 4 - High expression of recombinant human erythropoietin in plants using TMV and PVX-based viral vectors; Chapter 5 - Genetic stability and spatial distribution ofTobacco mosaic virus-based vectors in Nicotiana benthamiana plants; Chapter 6 - The nucleoprotein of Tomato spotted wilt virus as protein tag for easy purification and enhanced production of recombinant proteins in plants; Chapter 7 - General discussion; References. Summary. Samenvatting. About the author. Acknowledgements. Appendix (colou r figures).
Tipo: Tese/dissertação (ALICE) Palavras-chave: Viral vectors; Transient gene expression; Plant-made proteins; Erythropoietin; Gene fusion; Chicken anemia virus.
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/188426
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Performance comparison between broilers positive and negative for antibodies against the chicken anemia virus BJM
Rubin,Lauricio Librelotto; Ávila,Luiz Antônio Faccenda de; Ribeiro,Andréa Machado Leal; Wald,Vera; Canal,Cláudio Wageck.
The chicken anemia virus (CAV) is present in virtually every country investigated, Brazil including. The aim of this study was to determine what the difference in performance is between positive (progeny of breeders vaccinated or with natural infection) and negative broilers to the presence of antibodies against the CAV in currently intensive raising systems. As a result, it was observed that negative broilers were significantly heavier than positive broilers. Negative males had a final weight 5.43% higher than positive males. There was no significant difference among different treatments in relation to parameters as mortality and feeding conversion. These study indicated that the presence of antibodies against CAV in broilers - may it be through...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken anemia virus; Performance; Broiler; Vaccine; Antibody.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500030
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The occurrence of Orthoreovirus, Rotavirus and chicken anemia virus in chickens of the poultry industry in Minas Gerais, Brazil Arq. Bras. Med. Vet. Zootec.
Rios,R.L.; Marin,S.Y.; Gomes,A.M.; Resende,J.S.; Bonfim,M.R.Q.; Gomes,A.D.; Resende,F.M.S.; Horta,R.S.; Resende,M.; Martins,N.R.S..
Fifty-four fecal samples taken from broiler chickens from 1 to 45 days of age, and of pullets from 10 to 13 weeks of age, original from eight different poultry regions in the state of Minas Gerais, Brazil, were collected from March 2008 to January 2010 for avian Orthoreovirus (ARV) and avian Rotavirus (AvRV) analyses. For the assay of ARV, RNA was immediately extracted (Trizolâ) and transcribed into cDNA for assaying in a nested-PCR with ARV-specific primers. For AvRV, polyacrylamide gel electrophoresis (PAGE) was performed with RNA extracts obtained by phenol-chloroform extraction. CAV was additionally investigated through a nested-PCR of thymus and spleen. Results found 5.55% positive for ARV and 9.25% for AvRV. Also, CAV and ARV genomes were detected in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler; Layer; Chicken anemia virus; CAV; Avian reovirus; ARV; Avian rotavirus; AvRV; Nested-PCR; PAGE.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600030
Registros recuperados: 7
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