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A broad pH range and processive chitinase from a metagenome library BJMBR
Thimoteo,S.S.; Glogauer,A.; Faoro,H.; de Souza,E.M.; Huergo,L.F.; Moerschbacher,B.M.; Pedrosa,F.O..
Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene named metachi18A. The metachi18A gene was subcloned and overexpressed in Escherichia coli BL21 and the MetaChi18A chitinase was purified by affinity chromatography as a 6xHis-tagged fusion protein. The MetaChi18A enzyme is a 92-kDa protein with a conserved active site domain of glycosyl hydrolases family 18. It hydrolyses colloidal chitin with an optimum pH of 5 and temperature of 50°C. Moreover, the enzyme...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Metagenomic; Aeromonas; Kinetics.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100602
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Anti-oxidative and proteolytic activities and protein profile of laticifer cells of Cryptostegia grandiflora, Plumeria rubra and Euphorbia tirucalli Braz. J. Plant Physiol.
Freitas,Cleverson D. T. de; Souza,Diego P. de; Araújo,Eliane S.; Cavalheiro,Mariana G.; Oliveira,Luciana S.; Ramos,Márcio V..
In this study, proteins extracted from laticifer cells of three plants were examined by electrophoresis, mass spectrometry (MALDI-TOF) and characterized in respect of proteolytic, chitinolytic and anti-oxidative activities by means of zymography and colorimetric assays. Acidic proteins with molecular masses between 12.5 and 74.5 kDa predominated in laticifers of P. rubra. This profile was not found in laticifers of C. grandiflora and E. tirucalli. The later was poor in respect of proteins. Strong anti-oxidative activity of superoxide dismutase (E.C. 1.15.1.1) was detected in P. rubra and C. grandiflora latices, and to a lesser extent ascorbate peroxidase (E.C. 1.11.1.1) and isoforms of peroxidase were seen. Catalase (E.C. 1.11.1.6) was detected only in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catalase; Chitinase; Cysteine proteinase; Latex; Peroxidase.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000100002
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Applications of Plackett–Burman and Central Composite Design for the Optimization of Novel Brevundimonas diminuta KT277492 Chitinase Production, Investigation of its Antifungal Activity BABT
Warda,E. Ashour; Abeer,A. Abd El Aty; Eman,R. Hamed; Mahmoud,A. Swelim; Ahmed,I. El-Diwany.
ABSTRACT Biological control strategy which can damage chitin, a vital component of pathogenic fungi and arthropods promises a safe solution for many fungal problems. And it’s more favorable than chemicals which increase health risks and environmental problems. Thus, the chitinase producers appear potential candidates of biological control of pathogenic fungi. Brevundimonus diminuta KT277492 is a new isolate that has been isolated recently from Egyptian soil. Significant factors that affecting the chitinase enzyme production were studied and optimized using Plackett-Burman and Response Surface Methodology (RSM). As a result, maximum production of chitinase enzyme was 832.87 IUL-1, this result presented about 8.767-fold increase in the enzyme production. In...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Brevundimonas diminuta KT277492; Optimization; Plackett–Burman; Central composite design; Antifungal.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100429
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Atividade tóxica de isolados de Bacillus Thuringiensis a larvas de Aedes Aegypti (l.) (diptera: culicidae) Neotropical Entomology
Costa,Juliana R V da; Rossi,Juliana R; Marucci,Suzana C; Alves,Eliane C da C; Volpe,Haroldo X L; Ferraudo,Antonio S; Lemos,Manoel V F; Desidério,Janete A.
Aedes aegypti (L.), the main vector of dengue fever in Brazil, has been controlled with the use of massive chemical products, contributing to the development of resistance and decreasing the insect control efficiency. The control of dipterans with bioinsecticides based on Bacillus thuringiensis has been satisfactory, due to the production of insecticidal proteins denominated Cry (crystal), Cyt (cytolytic) toxins and Chi (chitinase), and to the synergistic effects among them. The present work aimed to select B. thuringiensis isolates efficient against A. aegypti larvae. A bacterial collection containing 1,073 isolates of B. thuringiensis, obtained from different locations of Brazilian territory, had the DNA isolated and submitted to PCR amplifications using...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Cry; Cyt; Chitinase; Dengue fever.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000500015
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Cepas nativas de Trichoderma spp., aisladas de suelo cultivado con jamaica, su antibiosis y micoparasitismo sobre Phytophthora parasitica y Fusarium oxysporum. Colegio de Postgraduados
Romero Rosales, Teolincacihuatl.
Se evaluaron cepas nativas de Trichoderma spp. aisladas de suelos cultivados con jamaica en Tecoanapa y Ayutla, Guerrero y con alta incidencia de Phytophthora parasitica y Fusarium oxysporum, involucrados en la "Pata prieta"; se realizó una cuantificación y actividad antagonica de quitinasas, glucanasas y 6PAP, y el estudio del micoparasitismo de cinco cepas de Trichoderma Ta10, Ta11, Ta6 y Ta9 las cuales pertenecen a la especie T. asperellum y Ti14 T. Inhamatum, sobre Phytophthora parasitica y Fusarium oxysporum. La producción de quitinasas en la actividad total osciló entre 0.06514 y 0.19288 µmol de N-acetilglucosamina h-1 y la actividad específica de 4.800 y 0.8917 µmol de N-acetilglucosamina h-1µg-1 proteína. La producción de glucanasas fue de 2.6283...
Palavras-chave: Control biológico; Quitinasas; Glucanasas; 6 pentil-α-pirona (6PAP); "Pata prieta"; Guerrero; Biological control; Chitinase; Glucanase; 6 pentyl-α-pyrone (6PAP); Fitopatología; Doctorado.
Ano: 2014 URL: http://hdl.handle.net/10521/2397
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Characterization of a chitinase with antifungal activity from a native Serratia marcescens B4A BJM
Zarei,Mandana; Aminzadeh,Saeed; Zolgharnein,Hossein; Safahieh,Alireza; Daliri,Morteza; Noghabi,Kambiz Akbari; Ghoroghi,Ahmad; Motallebi,Abbasali.
Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indicated an optimum activity in pH 5 at 45ºC. Enzyme was stable in 55ºC for 20 min and at a pH range of 3-9 for 90 min at 25ºC. When the temperature was raised to 60ºC, it might affect the structure of enzymes lead to reduction of chitinase activity. Moreover, the Km and Vmax values for chitin were 8.3 mg/ml and 2.4 mmol/min, respectively. Additionally, the effect of some cations and chemical compounds were found to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; Characterization; Chitinase; Serratia marcescens B4A.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300022
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Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity BABT
Vargas,Lúcia Rosane Bertholdo; Rossato,Marcelo; Ribeiro,Rute Terezinha da Silva; Barros,Neiva Monteiro de.
The characterization of entomopathogenic microorganisms is important for the selection of more effective strains for use in integrated pest-control programs. Five Nomuraea rileyi strains (SA86101, GU87401, SR86151, CG128 and VA9101) were characterized using random amplified polymorphic DNA (RAPD) analysis, virulence studies and assessment of chitinolytic and proteolytic activity. RAPD analysis divided the strains into two groups with a similarity coefficient of 0,76%, group 1 consisting of strains SA86101, GU87401 and SR86151 and group 2 of strains CG128 and VA9101. The LT50 varied from 165h with strain VA9101 to 246h with strain GU87401. Chitinolytic and proteolytic activity of the fungi after 144h growth in minimal medium were tested using colloidal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nomuraea rileyi; Anticarsia gemmatalis; Chitinase; Protease; RAPD; Virulence.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100003
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Chitinase activities, scab resistance, mycorrhization rates and biomass of own-rooted and grafted transgenic apple Genet. Mol. Biol.
Schäfer,Tina; Hanke,Magda-Viola; Flachowsky,Henryk; König,Stephan; Peil,Andreas; Kaldorf,Michael; Polle,Andrea; Buscot,François.
This study investigated the impact of constitutively expressed Trichoderma atroviride genes encoding exochitinase nag70 or endochitinase ech42 in transgenic lines of the apple cultivar Pinova on the symbiosis with arbuscular mycorrhizal fungi (AMF). We compared the exo- and endochitinase activities of leaves and roots from non-transgenic Pinova and the transgenic lines T386 and T389. Local and systemic effects were examined using own-rooted trees and trees grafted onto rootstock M9. Scab susceptibility was also assessed in own-rooted and grafted trees. AMF root colonization was assessed microscopically in the roots of apple trees cultivated in pots with artificial substrate and inoculated with the AMF Glomus intraradices and Glomus mosseae. Own-rooted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apple; Chitinase; Mycorrhiza; Transgenic.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300014
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Chitinase activity in rice in relation to infection by Rhizoctonia solani Kuhn International Rice Research Institute
Shrestha, Chandra Laxmi.
xx, 130 l. : ill. Thesis (Ph.D.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Chitinase; Rhizoctonia solani virus; Disease resistance; Enzymes.
Ano: 1996 URL: http://hdl.handle.net/123456789/600
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Chitinase and cellulase activity from Bacillus thuringiensis strains. Repositório Alice
DUMAS, V. F.; GOLDENBERG, C. doa S.; MARTINS, E. S.; PRACA, L. B.; QUEIROZ, P. R.; PONTES, R. G. M. S. de; MELO, F. R..
bitstream/item/183203/1/974-4593-1-PB.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cellulase; Cry proteins; Bacillus Thuringiensis; Chitinase.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/884059
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Chitinase by Bacillus spp. isolates and its efficacy against Fusarium moniliforme in maize. Repositório Alice
BRESSAN, W.; FIGUEIREDO, J. E. F..
2009
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Bacillus Fusarium; Chitinase; Biocontrol.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/490892
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Chitinase from Enterobacter sp. NRG4: Its purification, characterization and reaction pattern Electron. J. Biotechnol.
Dahiya,Neetu; Tewari,Rupinder; Tiwari,Ram P; Singh Hoondal,Gurinder.
Enterobacter sp. NRG4 was shown to excrete chitinase into the culture supernatant when cultivated in medium containing chitin. A 60 kDa extracellular chitinase was purified to homogeneity and characterized. The enzyme hydrolyzed swollen chitin, colloidal chitin, regenerated chitin and glycol chitin but did not hydrolyze chitosan. The chitinase exhibited Km and Vmax values of 1.43 mg ml-1 and 83.33 µM µg-1 h-1 for swollen chitin, 1.41 mg ml-1 and 74.07 µM µg-1 h-1 for colloidal chitin, 1.8 mg ml-1 and 40 µM µg-1 h-1 for regenerated chitin and 2.0 mg ml-1 and 33.33 µM µg-1 h-1 for glycol chitin, respectively. The optimal temperature and pH for activity were 45ºC and pH 5.5, respectively. Mg2+, K+ and Ca2+ stimulated chitinase activity by 13, 16 and 18%,...
Tipo: Journal article Palavras-chave: Chemical modification; Chitinase; Enterobacter sp. NRG4; Purification; Substrate binding.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200003
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Chitinase Production by Streptomyces sp. ANU 6277 BJM
Narayana,Kolla J.P.; Vijayalakshmi,Muvva.
Chitinase production by a terrestrial Streptomyces sp. ANU 6277 was studied under sub-merged fermentation. Chitinase production started after 24 h of incubation and reached maximum levels after 60 h of cultivation. A high level of chitinase activity was observed in the culture medium with pH 6 at 35ºC. Culture medium amended with 1% chitin was found to be suitable for maximum production of chitinase. An optimum concentration of colloidal chitin for chitinase production was determined. Studies on the influence of additional carbon and nitrogen sources on chitinase production revealed that starch and yeast extract served as good carbon and nitrogen sources to enhance chitinase yield.Chitinase was purified from crude enzyme extract by single step gel...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Streptomyces sp. ANU 6277; Optimization; Characterization.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400002
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Cloning and expression analysis of the chitinase gene Ifu-chit2 from Isaria fumosorosea Genet. Mol. Biol.
Meng,Huimin; Wang,Zhangxun; Meng,Xiangyun; Xie,Ling; Huang,Bo.
Entomopathogenic fungi can produce a series of chitinases, some of which function synergistically with proteases and other hydrolytic enzymes to degrade the insect cuticle. In the present study, the chitinase gene Ifu-chit2 from Isaria fumosorosea was investigated. The Ifu-chit2 gene is 1,435-bp long, interrupted by three short introns, and encodes a predicted protein of 423 amino acids with a 22 residue signal peptide. The predicted Ifu-Chit2 protein is highly homologous to Beauveria bassiana chitinase Bbchit2 and belongs to the glycohydrolase family 18. Ifu-Chit2 was expressed in Escherichia coli to verify chitinase activity, and the recombinant enzyme exhibited activity with a colloidal chitin substrate. Furthermore, the expression profiles of Ifu-chit2...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isaria fumosorosea; Chitinase; Prokaryotic expression; Quantitative real-time PCR.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300381
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Cloning of the Bacillus thuringiensis serovar sotto chitinase (Schi) gene and characterization of its protein Genet. Mol. Biol.
Zhong,Wan-Fang; Fang,Ji-Chao; Cai,Ping-Zhong; Yan,Wen-Zhao; Wu,Jie; Guo,Hui-Fang.
Chitinase plays a positive role in the pathogenicity of Bacillus thuringiensis to insect pests. We used touchdown PCR to clone the chitinase (Schi) gene from Bacillus thuringiensis serovar sotto (Bt sotto) chromosomal DNA. Our DNA sequencing analysis revealed that the Bt sotto Schi gene consists of an open reading frame (ORF) of 2067 nucleotides with codes for the chitinase precursor. We also found that the putative promoter consensus sequences (the -35 and -10 regions) of the Bt soto Schi gene are identical to those of the chiA71 gene from Bt Pakistani, the chiA74 gene from Bt kenyae and the ichi gene from Bt israelensis. The Schi chitinase precursor is 688 amino acids long with an estimated molecular mass of 75.75 kDa and a theoretical isoelectric point...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus thuringiensis serovar sotto; Chitinase; Touchdown PCR; Gene cloning; Characterization analysis.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500026
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Comparison of isozyme transformation in maize as a result of insertion of the chitinase gene Phyton
Yan,PM; Zhang,HF; Wang,Q; Yan,XY; Sun,Y.
Isozymes of peroxidase (POD), catalase (CAT), esterase (EST) and superoxide dismutase (SOD) were analyzed on transgenic maize (with external chitinase gene) and its parent by vertical polyacrylamide gel electrophoresis (PAGE). This study was made using shoots at the fourth leaf stage. Results showed that: POD and EST were detected in 6 bands. POD-2 and POD-3 were present at the bud and seedling stages. POD-1, POD-4, POD-5 and POD-6 were only present at the seedling stage. POD-6 expressed stronger in the transgenic maize with chitinase than in its parent. EST-2 was present only at the bud stage, and its expression in transgenic maize was stronger than that in its parent. EST-5 only existed at the seedling stage. EST-4 did not exist in the parent maize...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Isozyme; Maize; Antioxidant enzyme system.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200002
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Efecto de quitina y quitosano sobre huevos y juveniles de nemátodos formadores de nódulos radiculares, Nacobbus aberrans y Meloidogyne incognita bajo condiciones de in vitro e in vivo Colegio de Postgraduados
Sánchez Portillo, Juan Fernando.
El nematodo agallador Meloidogyne incognita Kofoid y White, 1919, Chitwood, 1949, distribuido en 23 de los 32 estados del país y Nacobbus aberrans Thorne 1935, Thorne and Allen, 1944, distribuido en 10 de 32 estados de la República Mexicana, su combate para ambas especies, casi siempre se hace aplicando diferentes agroquímicos, los cuales algunos ya tienen problemas de registro. Se necesita evaluar nuevos productos que sean otra alternativa a su manejo y mas amigables al ambiente. En el presente trabajo se evaluó el efecto de quitina-quitosano con diferentes grados de desacetilación, biopolímeros naturales que poseen propiedades nematicidas y utilizados como reguladores de la población de estos fitonematodos; los estudios se hicieron in vitro, sobre la...
Palavras-chave: Biopolímero natural; Meloidogyne incognita; Nacobbus aberrans; Desacetilación; Control; Quitina; Quitosano; Chitin; Chitinase; Natural biopolymer; Chitosan; Deacetylation; Fitopatología; Maestría.
Ano: 2010 URL: http://hdl.handle.net/10521/55
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Efecto de quitina y quitosano sobre huevos y juveniles de nemátodos formadores de nódulos radiculares, Nacobbus aberrans y Meloidogyne incognita bajo condiciones de in vitro e in vivo Colegio de Postgraduados
Sánchez Portillo, Juan Fernando.
El nematodo agallador Meloidogyne incognita Kofoid y White, 1919, Chitwood, 1949, distribuido en 23 de los 32 estados del país y Nacobbus aberrans Thorne 1935, Thorne and Allen, 1944, distribuido en 10 de 32 estados de la República Mexicana, su combate para ambas especies, casi siempre se hace aplicando diferentes agroquímicos, los cuales algunos ya tienen problemas de registro. Se necesita evaluar nuevos productos que sean otra alternativa a su manejo y mas amigables al ambiente. En el presente trabajo se evaluó el efecto de quitina-quitosano con diferentes grados de desacetilación, biopolímeros naturales que poseen propiedades nematicidas y utilizados como reguladores de la población de estos fitonematodos; los estudios se hicieron in vitro, sobre la...
Palavras-chave: Biopolímero natural; Meloidogyne incognita; Nacobbus aberrans; Desacetilación; Control; Quitina; Quitosano; Chitin; Chitinase; Natural biopolymer; Chitosan; Deacetylation; Fitopatología; Maestría.
Ano: 2010 URL: http://hdl.handle.net/10521/55
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Enzymatic comparison and mortality of Beauveria bassiana against cabbage caterpillar Pieris brassicae LINN BJM
Dhawan,Manish; Joshi,Neelam.
Abstract Beauveria bassiana, an entomopathogenic fungus, is the alternative biocontrol agent exploited against major economic crop pests. Pieris brassicae L. is an emerging pest of the Brassicaceae family. Therefore, in the present study, fungal isolates of Beauveria bassiana, viz. MTCC 2028, MTCC 4495, MTCC 6291, and NBAII-11, were evaluated for their virulence against third instar larvae of P. brassicae. Among all these fungal isolates, maximum mortality (86.66%) was recorded in B. bassiana MTCC 4495 at higher concentration of spores (109 conidia/ml), and the minimum mortality (30.00%) was recorded in B. bassiana MTCC 6291 at a lower concentration (107 conidia/ml) after ten days of treatment. The extracellular cuticle-degrading enzyme activities of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Entomopathogenic fungi; Lipase; Mortality; Protease.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300522
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Expression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation. Repositório Alice
BOSCO, D. D.; SINSKI, I.; RITSCHEL, P. S.; CAMARGO, U. A.; FAJARDO, T. V. M.; HARAKAVA, R.; QUECINI, V..
Abstract Increased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Pathogenesis related protein 5; Fungus; Chitinase; Epigenetics; Grapevine leafroll-associated virus 3; Vitis.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1092380
Registros recuperados: 32
Primeira ... 12 ... Última
 

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