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Registros recuperados: 17
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A rapid alternative technique for obtaining silver-positive patterns in chromosomes Genet. Mol. Biol.
Kavalco,Karine Frehner; Pazza,Rubens.
Silver nitrate chromosome staining to evidence nucleolar organizer regions (NORs) is a widely adopted methodology. The aim of the present work was to improve this technique, reducing the preparation time without decreasing the quality of the results. Microwave irradiation proved to be quite efficient and reliable for this purpose, as it allowed to identify Ag-NORs equivalent to those obtained by the conventional procedure and also to reduce the concentration of the employed reagents, as well as the precipitation of debris on the preparation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Silver staining; Ag-NOR; Microwaves; Chromosome banding.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200012
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Chromosomal organization and phylogenetic relationships in Hypochaeris species (Asteraceae) from Brazil Genet. Mol. Biol.
Ruas,Claudete de Fátima; Vanzela,André L.L.; Santos,Melissa O.; Fregonezi,Jeferson N.; Ruas,Paulo Maurício; Matzenbacher,Nelson I.; Aguiar-Perecin,Margarida L.R. de.
The association of cytogenetic and molecular techniques has contributed to the analysis of chromosome organization and phylogeny in plants. The fluorochrome GC-specific CMA3, fluorescent in situ hybridization (FISH) and RAPD (Random Amplified Polymorphic DNA) markers were used to investigate chromosome structure and genetic relationships in Hypochaeris (Asteraceae). Seven species native to South America, and two species introduced from Europe (H. glabra and Hypochaeris sp) were studied. FISH with rDNA probes identified one or two loci of 18S-5.8S-25S rDNA in the South American Hypochaeris species and one locus in the European species. Only one 5S rDNA locus was seen in all species studied. Blocks of GC-rich heterochromatin (CMA-positive bands) associated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome banding; FISH; Hypochaeris; Phylogenetic relationship; RAPD.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100023
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Comparative cytogenetic analysis between Lonchorhina aurita and Trachops cirrhosus (Chiroptera, Phyllostomidae) Genet. Mol. Biol.
Barros,Helen Maria Duarte do Rêgo; Sotero-Caio,Cibele Gomes de; Santos,Neide; Souza,Maria José de.
Phyllostomidae comprises the most diverse family of neotropical bats, its wide range of morphological features leading to uncertainty regarding phylogenetic relationships. Seeing that cytogenetics is one of the fields capable of providing support for currently adopted classifications through the use of several markers, a comparative analysis between two Phyllostomidae species was undertaken in the present study, with a view to supplying datasets for the further establishment of Phyllostomidae evolutionary relationships. Karyotypes of Lonchorhina aurita (2n = 32; FN = 60) and Trachops cirrhosus (2n = 30; FN = 56) were analyzed by G- and C-banding, silver nitrate staining (Ag-NOR) and base-specific fluorochromes. Chromosomal data obtained for both species...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bats; Chromosome banding; Fluorochromes; NOR.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400014
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Comparative cytogenetics of Hoplias malabaricus (Pisces, Erythrinidae): a population analysis in adjacent hydrographic basins Genet. Mol. Biol.
Vicari,Marcelo Ricardo; Artoni,Roberto Ferreira; Bertollo,Luiz Antonio Carlos.
The chromosomes of specimens from four Hoplias malabaricus populations from headwaters of adjacent river basins at Ponta Grossa, southern Brazil, were investigated using differential staining techniques (C-banding, AgNO3 and CMA3) and fluorescent in situ hybridization (FISH) with an 18S rDNA probe. The diploid chromosome number in representatives of all four populations was invariably 2n = 42, with karyotypes composed of 12 pairs of metacentrics and 9 pairs of submetacentrics, without heteromorphic sex chromosomes. This kind of karyotype represents cytotype A in regard to cytotypes identified previously in H. malabaricus, exhibiting however, at the same time, some differences in the distribution of constitutive heterochromatin segments and in the locations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome banding; FISH; Cytotaxonomy of trahiras; Karyotype; NOR phenotype.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100018
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Cytogenetic comparison of Podocnemis expansa and Podocnemis unifilis: a case of inversion and duplication involving constitutive heterochromatin Genet. Mol. Biol.
Gunski,Ricardo José; Cunha,Isabel Souza; Degrandi,Tiago Marafiga; Ledesma,Mario; Garnero,Analía Del Valle.
Podocnemis expansa and P. unifilis present 2n = 28 chromosomes, a diploid number similar to those observed in other species of the genus. The aim of this study was to characterize these two species using conventional staining and differential CBG-, GTG and Ag-NOR banding. We analyzed specimens of P. expansa and P. unifilis from the state of Tocantins (Brazil), in which we found a 2n = 28 and karyotypes differing in the morphology of the 13th pair, which was submetacentric in P. expansa and telocentric in P. unifilis. The CBG-banding patterns revealed a heterochromatic block in the short arm of pair 13 of P. expansa and an interstitial one in pair 13 of P. unifilis, suggesting a pericentric inversion. Pair 14 of P. unifilis showed an insterstitial band in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome banding; Ag-NORs; Karyotype; Chelonids.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300009
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Cytogenetic studies in three species of the genus Oligosarcus BABT
Rubert,Marceléia; Margarido,Vladimir Pavan.
Karyotypic studies were carried out on three species of the genus Oligosarcus: O. paranensis, O. pintoi, and O. longisrostris. All of them showed a diploid number of 50 chromosomes and the same karyotypic formulae (4M + 10SM + 16ST + 20A). Silver nitrate staining revealed single NORs in O. longirostris and multiple NORs in O. paranensis and O. pintoi. Heterochromatin seemed to play an important role in the chromosomal diversification of these species. Based on cytogenetical data, speciation hypotheses within this group were proposed, reinforcing the importance of chromosomal studies for a better understanding of evolution within the genus Oligosarcus, as well as within the family Characidae.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetic studies; Oligosarcus; Acestrorhynchinae; Chromosome banding.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100015
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Cytogenetical analyses in three fish species of the genus Pimelodus(Siluriformes: Pimelodidae) from rio São Francisco: considerations about the karyotypical evolution in the genus Neotropical Ichthyology
Garcia,Caroline; Moreira Filho,Orlando.
Karyotypes and other chromosomal markers were investigated in three species of the catfish genus Pimelodus, namely P. fur, P. maculatus and Pimelodus sp., from municipality of Três Marias, Minas Gerais, Brazil, using differential staining techniques (C-banding, Silver nitrate and CMA3 staining). The diploid chromosome number was 2n = 56 in P. maculatus and Pimelodus sp., while in P. fur 2n = 54. The karyotype of P. fur consisted in 32M + 8SM + 6ST + 8A with fundamental number (NF) of 100, that of P. maculatus 32M + 12SM + 12A with NF = 112, and that of Pimelodus sp. had 32M + 12Sm + 6ST + 6A with NF = 106.The nucleolar organizer regions (NORs) in all three species were invariably detected in telomeres of longer arm of the 20th chromosome pair. These sites...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish cytogenetics; Diploid number reduction; Chromosomal rearrangements; Chromosome banding; Catfishes.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252005000200006
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Cytogenetics of Chilean angiosperms: Advances and prospects RChHN
JARA-SEGUEL,PEDRO; URRUTIA,JONATHAN.
Cytogenetic data on Chilean angiosperms have been reported since at least eight decades ago; however, much of this information is disperse in diverse sources and is not readily available as a comprehensive document that allows having a general vision on advances and gaps in this matter. The goal of this paper is to summarize the advances and prospets on cytogenetic studies of the Chilean angiosperms based on compiled publications from 1929 to 2010. We found 78 publications supplied by four groups of Chilean researchers and some foreign specialists. Cytogenetic data have been reported for 139 Chilean angiosperm species (2.8 % of the total), which belong to 58 genera and 34 families. During 2001-2010 there was an increase in the number of publications, being...
Tipo: Journal article Palavras-chave: Chromosome banding; Chromosome number; Genome size; Karyotype morphology.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2012000100001
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Cytogenetics of coffee Braz. J. Plant Physiol.
Pinto-Maglio,Cecília A. F..
The genus Coffea L. has around 100 native species distributed in tropical and subtropical areas in Africa, and the most important economic species are C. arabica and C. canephora. C. arabica is exceptional in the genus since it is the only species so far analyzed which is self-compatible, and a natural polyploid with 2n=4x=44 chromosomes; it is considered to be a segmental allopolyploid because it presents a disomic inheritance and a regular meiotic behavior. All other species in the genus are self-incompatible diploids with 2n=2x=22. Cytogenetic studies in Coffea, undertaken since 1912, have followed various phases: initial studies were limited only to establishing chromosome counts. Subsequent studies characterized the karyotypes of various species using...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea; Chromosomal characterization; Karyotype; Meiotic behavior; Chromomere pattern; Chromosome banding; Molecular cytogenetics.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100004
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Identification of naturalized goat breeds under conservation from northeastern Brazil using chromosomal markers. Repositório Alice
SANTOS, R. P.; AFFONSO, P. R. A. M.; DINIZ, D.; MEDRADO, A. S.; SILVA, K. de M.; CARNEIRO, P. L. S..
Abstract: In order to provide the first cytogenetic data of naturalized and threatened goat breeds from northeastern Brazil, cytogenetic analyses were carried out in individuals of Repartida and Moxotó breeds raised in Bahia and Ceará States. Males and females of both breeds had 2n = 60, with 29 autosomal acrocentric pairs plus the sex chromosome pair. The number of nucleolar organizer region (NOR)-bearing chromosomes ranged from 6 to 8 per metaphase in Moxotó and Repartida goats, respectively. The active NORs in Repartida individuals were located exclusively at the terminal regions of the long arms, as usually detected in Bovidae. Otherwise, Moxotó specimens presented a large autosomal pair with NORs on short arms. GC-rich heterochromatin was detected at...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bandeamento cromossômico; Raça naturalizada; Brasil; Região Nordeste; Caprino; Melhoramento genético animal; Raça Moxotó; Citogenética animal; Genética animal; Cariótipo; Cromossoma; Conservação; Goats; Animal genetics; Genetic improvement; Karyotypes; Chromosome banding; Brazil.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/969537
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Interspecific hybridization in oysters: Restriction enzyme digestion chromosome banding confirms Crassostrea angulata x Crassostrea gigas F1 hybrids ArchiMer
Leitao, A; Chaves, R; Santos, S; Guedes Pinto, H; Boudry, Pierre.
The taxonomic status of the two commercially important cupped oysters, Crassostrea angulata, the Portuguese oyster (Lamarck, 1819) and Crassostrea gigas, the Japanese oyster (Thunberg, 1793) has long been in question. The recent observation of the hybridization between C. gigas and C. angulata and the production of fertile F1s led us to search for cytogenetic evidence of both parental genomes in the interspecific hybrids. The cytogenetic characterization of the hybrids was performed by the use of restriction endonuclease treatments. This technique has recently shown the potential for individual chromosome identification by banding in oysters. Chromosomes of C. gigas, C. angulata and their hybrids were treated with two different restriction enzymes (ApaI...
Tipo: Text Palavras-chave: Interspecific hybrids; In situ restriction enzyme banding; Crassostrea gigas; C. angulata; Chromosome banding.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4959.pdf
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Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA Genet. Mol. Biol.
Fregonezi,Jéferson Nunes; Fernandes,Thiago; Torezan,José Marcelo Domingues; Vieira,Ana Odete S.; Vanzela,André Luís Laforga.
We studied the karyotypes of four Brazilian Cestrum species (C. amictum, C. intermedium, C. sendtnerianum and C. strigilatum) using conventional Feulgen staining, C-Giemsa and C-CMA3/DAPI banding, induction of cold-sensitive regions (CSRs) and fluorescent in situ hybridization (FISH) with rDNA probes. We found that the karyotypes of all four species was 2n = 2x = 16, with, except for the eighth acrocentric pair, a predominance of meta- and submetacentric chromosomes and various heterochromatin classes. Heterochromatic types previously unreported in Cestrum as neutral C-CMA3(0)/DAPI0 bands, CMA3+ bands not associated with NORs, and C-Giemsa/CSR/DAPI- bands were found. The heterochromatic blocks varied in size, number, position and composition. The 45S rDNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cestrum; Chromosome banding; CSRs; Karyotype evolution; RDNA probes.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100019
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Karyotype differentiation of four Cestrum species (Solanaceae) revealed by fluorescent chromosome banding and FISH Genet. Mol. Biol.
Fernandes,Thiago; Rego,Letícia do Nascimento Andrade de Almeida; Nardy,Mariana; Yuyama,Priscila Mary; Vanzela,André Luís Laforga.
The karyotypes of four South American species of Cestrum (C. capsulare, C. corymbosum, C. laevigatum and C. megalophylum) were studied using conventional staining, C-CMA/DAPI chromosome banding and FISH with 45S and 5S rDNA probes. The karyotypes showed a chromosome number of 2n = 2x = 16, with metacentric chromosomes, except for the eighth submeta- to acrocentric pair. Several types of heterochromatin were detected, which varied in size, number, distribution and base composition. The C-CMA+ bands and 45S rDNA were located predominantly in terminal regions. The C-CMA+/DAPI+ bands appeared in interstitial and terminal regions, and the C-DAPI+ bands were found in all chromosome regions. The 5S rDNA sites were observed on the long arm of pair 8 in all species...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cestrum; Chromosome banding; FISH; Heterochromatin; Physical maps; 45S and 5S rDNA.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200019
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Karyotype diversity between species of Crenicichla (Perciformes, Cichlidae) from different Brazilian hydrographic basins Genet. Mol. Biol.
Pires,Larissa Bettin; Usso,Mariana Campaner; Giuliano-Caetano,Lucia; Dias,Ana Lúcia.
Abstract Crenicichla is the largest genus in the Cichlidae family in South America. The genus includes 100 valid species that are popularly known in Brazil as jacundás or joaninhas and are widely distributed in rivers east of the Andes. Cytogenetic analyses were carried out on seven species in this genus. All species showed a diploid number of 48 with interspecific differences in karyotype formulas and AgNORs located in interstitial position on the short arm of the largest metacentric pair, except for the two populations from C. britskii. Population A showed terminal markings on the long arm of the fifth pair of the complement, and population B showed up to two marked chromosome pairs. FISH with an 18S rDNA probe was coincident with AgNORs and CMA3, except...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome banding; Fish cytogenetics; Geophaginae; Ribosomal DNA.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300357
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Karyotypic differentiation through chromosome fusion and number reduction in Imparfinis hollandi (Ostariophysi, Heptapteridae) Genet. Mol. Biol.
Margarido,Vladimir Pavan; Moreira-Filho,Orlando.
The Neotropical Heptapteridae fish Imparfinis hollandi, endemic to the Iguaçu River Basin (Brazil), was cytogenetically analyzed and the diploid chromosome number of 2n = 42 chromosomes was determined (22m + 10sm + 10st), the lowest diploid number in this genus and family. Like other Heptapteridae species, only one NOR-bearing chromosome pair was detected by silver nitrate staining. Dark heterochromatic blocks were visualized in only three chromosome pairs, and chromomycin A3+ bands were coincident with Ag-NORs. Although no intercalary (TTAGGG)n sequence was observed through FISH with a telomere probe, an asymmetric karyotype showing four large chromosome pairs with diploid chromosome number reduction suggests that tandem chromosome fusions probably...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Siluriformes; Chromosome banding; Chromosomal rearrangements; (TTAGGG)n.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200012
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Mapping of ribosomal genes and chromosomal markers in three species of the genus Serrasalmus (Characidae, Serrasalminae) from the Amazon basin Genet. Mol. Biol.
Nakayama,Celeste M.; Feldberg,Eliana; Bertollo,Luiz Antonio C..
Karyotypic characteristics of three species of the genus serrasalmus ( S. altispinnis , S. gouldingi and S. Serrulatus ) from the middle and lower Negro River, Amazon Basin, were investigated using different staining techniques and Fluorescent in situ hybridization with 5S and 18S rDNA probes. The diploid number was invariably 2n = 60 and the fundamental number was FN = 110. Nevertheless, the karyotypes differed from each other in composition: 24m, 20sm, 6st, 10a in S. altispinnis ; 22m, 22sm, 6st, 10a in S. gouldingi and 20m, 22sm, 8st, 10a in S. serrulatus. The karyotype of S. altispinnis differed from the one previously described in a population from the Pitinga River. C-positive constitutive heterochromatin was mainly pericentromeric in...
Palavras-chave: Chromosome banding; Cytotaxonomy of piranhas; FISH; Karyotype; Ribosomal DNA.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500011
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rDNA mapping, heterochromatin characterization and AT/GC content of Agapanthus africanus (L.) Hoffmanns (Agapanthaceae) Anais da ABC (AABC)
REIS,ARYANE C.; FRANCO,ANA LUIZA; CAMPOS,VICTÓRIA R.; SOUZA,FLÁVIA R.; ZORZATTO,CRISTIANE; VICCINI,LYDERSON F.; SOUSA,SAULO M..
ABSTRACT Agapanthus (Agapanthaceae) has 10 species described. However, most taxonomists differ respect to this number because the great phenotypic plasticity of the species. The cytogenetic has been an important tool to aid the plant taxon identification, and to date, all taxa of Agapanthus L'Héritier studied cytologically, presented 2n = 30. Although the species possess large chromosomes, the group is karyologically little explored. This work aimed to increase the cytogenetic knowledge of Agapanthus africanus (L.) Hoffmanns by utilization of chromosome banding techniques with DAPI / CMA3 and Fluorescent in situ Hybridization (FISH). In addition, flow cytometry was used for determination of DNA content and the percentage of AT / GC nitrogenous bases....
Tipo: Info:eu-repo/semantics/article Palavras-chave: African lily; Chromosome banding; Cytotaxonomy; Flow cytometry; Genome size; Molecular cytogenetics.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501727
Registros recuperados: 17
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