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Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)(4), (GT)(7) and (TA)(10) chromosome patterns ArchiMer
Bouilly, Karine; Chaves, R.; Leitao, A.; Benabdelmouna, Abdellah; Guedes Pinto, H..
Chromosome identification is essential in oyster genomic research. Fluorescence in situ hybridization (FISH) offers new opportunities for the identification of oyster chromosomes. It has been used to locate satellite DNAs, telomeres or ribosomal DNA sequences. However, regarding chromosome identification, no study has been conducted with simple sequence repeats (SSRs). FISH was used to probe the physical organization of three particular SSRs, (GGAT)(4), (GT)(7) and (TA)(10) onto metaphase chromosomes of the Pacific oyster, Crassostrea gigas. Hybridization signals were observed in all the SSR probes, but the distribution and intensity of signals varied according to the oligonucleotide repeat. The intercalary, centromeric and telomeric bands were observed...
Tipo: Text Palavras-chave: Simple sequence repeats (SSRs); Mollusks; Fluorescence in situ hybridization (FISH); Crassostrea gigas; Chromosome identification.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6132.pdf
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