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| Registros recuperados: 10 | |
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| Sastre,Darío A.; Argaraña,Carlos E.; Heller,Viviana B.; Gallo,Mónica; Fernández,Enrique N.; Rodríguez,Cecilia M.. |
| In this work, we describe the advantages of multiplex-PCR in the specific detection of BCR-ABL transcripts in different hematological disorders and its sensitivity compared to nested PCR. Fifty-three patients were studied for the presence of BCR-ABL transcripts: 24 patients with chronic myeloid leukemia (CML), 20 with acute leukemia (AL), and 9 patients with other hematological disorders. A variant rearrangement (b3a3) was found in a single case of CML (4.2%). Four out of the 20 patients with AL (20.0%) (14 adults, 6 children) were bcr-abl(+), and in this group three cases were classified as B-acute lymphoblastic leukemia (B-ALL), and one as acute myeloblastic leukemia (AML). Two of the three patients with B-ALL were positive for b2a2 and the other one for... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Multiplex-PCR; Ph chromosome; BCR-ABL; Chronic myeloid leukemia; PCR. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400003 |
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| Otero,Luize; Cavalcanti Júnior,Geraldo Barroso; Klumb,Claudete Esteves; Scheiner,Marcos Antonio Mauricio; Magluta,Eliane Pereira Simões; Fernandez,Teresa de Souza; Silva,Maria Luiza Macedo; Pires,Virgínia; Andrade,Gabriela Vasconcelos; Maia,Raquel Ciuvalschi; Tabak,Daniel. |
| chronic myeloid leukemia (CML) have been described. This chromosomal region contains the tumor suppressor gene TP53 that may be an important factor in the evolution of this disease. In this study, we used flow cytometry and western blotting to assess p53 protein expression and single stranded conformational polymorphism to examine TP53 gene alterations in three patients with CML who showed alterations in 17p. Only the case with del(17)(p11) had p53 expression positive by flow cytometry and an abnormal migration pattern by SSCP analysis. The importance of the correlation between the results obtained with these techniques, as well as the clinical course of the patients, are discussed. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chronic myeloid leukemia; Chromosome 17; TP53 gene; P53 protein. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100007 |
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| Godoy,C.R.T.; Levy,D.; Giampaoli,V.; Chamone,D.A.F.; Bydlowski,S.P.; Pereira,J.. |
| We measured circulating endothelial precursor cells (EPCs), activated circulating endothelial cells (aCECs), and mature circulating endothelial cells (mCECs) using four-color multiparametric flow cytometry in the peripheral blood of 84 chronic myeloid leukemia (CML) patients and 65 healthy controls; and vascular endothelial growth factor (VEGF) by quantitative real-time PCR in 50 CML patients and 32 healthy controls. Because of an increase in mCECs, the median percentage of CECs in CML blast crisis (0.0146%) was significantly higher than in healthy subjects (0.0059%, P<0.01) and in the accelerated phase (0.0059%, P=0.01). There were no significant differences in the percentages of CECs in chronic- or active-phase patients and healthy subjects... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Circulating endothelial cells; Vascular endothelial growth factor; Chronic myeloid leukemia; Flow cytometry; Angiogenesis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600509 |
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| Benati,Rogério Bodini; Costa,Tássia Rafaela; Cacemiro,Maira da Costa; Sampaio,Suely Vilela; Castro,Fabíola Attié de; Burin,Sandra Mara. |
| Abstract Background: Chronic myeloid leukemia (CML) is a BCR-ABL1+ myeloproliferative neoplasm marked by increased myeloproliferation and presence of leukemic cells resistant to apoptosis. The current first-line therapy for CML is administration of the tyrosine kinase inhibitors imatinib mesylate, dasatinib or nilotinib. Although effective to treat CML, some patients have become resistant to this therapy, leading to disease progression and death. Thus, the discovery of new compounds to improve CML therapy is still challenging. Here we addressed whether MjTX-I, a phospholipase A2 isolated from Bothrops moojeni snake venom, affects the viability of imatinib mesylate-resistant Bcr-Abl+ cell lines. Methods: We examined the cytotoxic and pro-apoptotic effect... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chronic myeloid leukemia; Bcr-Abl; Phospholipase A2; MjTX-I; Bothrops moojeni; Apoptosis; Cytotoxicity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100330 |
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| Ghadage,D.P.; Bal,A.M.. |
| Splenic abscesses are caused by Staphylococcus aureus, Streptococcus and bacteria belonging to the family Enterobacteriaceae. We report a case of splenic abscess caused by an unusual serotype of Salmonella. A 55 year old man was admitted with complaints of fever and abdominal pain. On the basis of clinical findings and laboratory reports, a diagnosis of chronic myeloid leukemia was made. Ultrasonography of the abdomen revealed a single large cystic lesion in the spleen. Percutaneous drainage of the abscess was carried out. Salmonella enterica serotype Worthington was isolated from a pus sample taken from the abscess. The isolate was resistant to ampicillin, gentamicin, cefotaxime, chloramphenicol and tetracycline, and sensitive to amikacin and norfloxacin.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chronic myeloid leukemia; Splenic abscess; Worthington. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000200005 |
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| Carrara,R.C.V.; Orellana,M.D.; Fontes,A.M.; Palma,P.V.B.; Kashima,S.; Mendes,M.R.; Coutinho,M.A.; Voltarelli,J.C.; Covas,D.T.. |
| Bone marrow is a heterogeneous cell population which includes hematopoietic and mesenchymal progenitor cells. Dysregulated hematopoiesis occurs in chronic myelogenous leukemia (CML), being caused at least in part by abnormalities in the hematopoietic progenitors. However, the role of mesenchymal stem cells (MSCs) in CML has not been well characterized. The objectives of the present study were to observe the biological characteristics of MSCs from CML patients and to determine if MSCs originate in part from donors in CML patients after bone marrow transplantation (BMT). We analyzed MSCs from 5 untreated patients and from 3 CML patients after sex-mismatched allogeneic BMT. Flow cytometry analysis revealed the typical MSC phenotype and in vitro assays showed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mesenchymal stem cells; Chimerism; Allogeneic hematopoietic stem cell transplantation; Chronic myeloid leukemia; Bcr-abl gene. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100008 |
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| Oliveira,G.A.P.; Costa,E.S.; Freitas,M.S.; Dutra,F.F.; Maia,S.F.; Guerra,M.C.; Tabernero,M.D.; Borojevic,R.; Otazu,I.B.; Silva,J.L.. |
| Chronic myeloid leukemia (CML) is rare in the pediatric population, accounting for 2-3% of childhood leukemia cases, with an annual incidence of one case per million children. The low toxicity profile of imatinib mesylate has led to its approval as a front-line therapy in children for whom interferon treatment has failed or who have relapsed after allogeneic transplantation. We describe the positive responses of 2 children (case 1 - from a 7-year-old male since May 2005; case 2 - from a 5-year-old female since June 2006) with Philadelphia-positive chromosome CML treated with imatinib (300 mg/day, orally) for up to 28 months, as evaluated by morphological, cytogenetic, and molecular approaches. Our patients are alive, are in the chronic phase, and are in... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Chronic myeloid leukemia; Imatinib mesylate; Minimal residual disease; Children. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600009 |
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| Nunes,Melise Silveira; Garzon,Litiérri Razia; Rampelotto,Roberta Filipini; Tizotti,Maísa Kräulich; Martini,Rosiéli; Locatelli,Aline; Barbosa,Marcelo; Hörner,Manfredo; Hörner,Rosmari. |
| ABSTRACT The enhancement of anti-leukemia therapy and the treatment of infections caused by multidrug-resistant pathogens are major challenges in healthcare. Although a large arsenal of drugs is available, many of these become ineffective, and as a result, the discovery of new active substances occurs. Notably, triazenes (TZCs) have been consolidated as a promising class of compounds, characterized by significant biological activity, especially antiproliferative and antimicrobial properties. The aim of this study is the synthesis and characterization of a new triazenide complex of gold (I), as well as the in vitro assessment of its antiproliferative activity against the K562 cell line (Chronic Myeloid Leukemia), and antibacterial activity against bacterial... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Triazenes/synthesis/characterization/biological activity; TZC/Gold ion complexed; Chronic myeloid leukemia; Antibacterial activity. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400617 |
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| Registros recuperados: 10 | |
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