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Registros recuperados: 61
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A legume genomics resource: The Chickpea Root Expressed Sequence Tag Database Electron. J. Biotechnol.
Jayashree,B; Buhariwalla,Hutokshi K; Shinde,Sanjeev; Crouch,Jonathan H.
Chickpea, a lesser-studied grain legume, is being investigated due to its taxonomic proximity with the model legume genome Medicago truncatula and its ability to endure and grow in relatively low soil water contents making it a model legume crop for the study of agronomic response to drought stress. Public databases currently contain very few sequences from chickpea associated with expression in root tissues. However, root traits are likely to be one of the most important components of drought tolerance in chickpea. Thus, we have generated a set of over 2800 chickpea expressed sequence tags (ESTs) from a library constructed after subtractive suppressive hybridization (SSH) of root tissue from two closely related chickpea genotypes possessing different...
Tipo: Journal article Palavras-chave: Cloning; Data mining; Drought avoidance; Drought tolerance; EST database; Root traits; Stress.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200002
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Advances in the propagation of Jabuticaba tree Rev. Bras. Frutic.
Silva,José Antonio Alberto da; Teixeira,Gustavo Henrique de Almeida; Martins,Antonio Baldo Geraldo; Citadin,Idemir; Wagner Júnior,Américo; Danner,Moeses Andrigo.
Abstract The Myrtaceae family has representatives spread worldwide, and Jabuticaba tree is an important species native to Brazil, which is grown not only in the backyards and domestic orchards, but also in commercial orchards, landscaping projects and in several tropical and subtropical countries. The propagation of this species in a sexual form or by seeds is simple and efficient, being able to reach 100%. However, long juvenile phase and genetic segregation have led to the search for methods that allow the propagation of selected trees and guarantee through their standardized and productive clones, fruit quality and fruiting precocity. Protocols for asexual propagation are still scarce, but they show efficient methods, making possible the commercial use...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Myrtaceae; Plinia sp.; Grafting; Cutting; Air layering; Cloning.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000301007
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Advances in the spread of vegetative blueberry Rev. Bras. Frutic.
Schuch,Márcia Wulff; Tomaz,Zeni Fonseca Pinto.
Abstract The lack of an efficient method of propagation in some cultivars makes it difficult to expand the blueberry culture. In Brazil, we still do not produce seedlings with needed quality and quantity to improve the sector. The cloning of the selected material through mini propagation and micro propagation is an alternative to increase the multiplication and rooting capacity of the blueberries. Allied to this, the use of cultivation system without soil for the development of cloned material facilitates the seedlings production. In addition, we can keep matrices in small spaces with the use of mini-gardens and clonal micro-gardens controlling climatic and nutritional factors. In the Laboratory of Fruit Plants Propagation, of the Phytotechnology...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Seedlings; Cloning; Mini propagation and micro propagation; Cultivation system without soil.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000104001
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Advances in vegetative propagation of Olive tree Rev. Bras. Frutic.
Schuch,Márcia Wulff; Tomaz,Zeni Fonseca Pinto; Casarin,Josiane Vergara; Moreira,Roseane Maidana; Silva,Jacqueline Barcelos da.
Abstract In Brazil, there is a growing interest in the crop of the olive tree, especially in those regions considered suitable for cultivation, where low temperatures occur between 8ºC and 10ºC in the period before flowering. The areas with these characteristics are located mainly in the South and Southeast regions of the country, characterized by a temperate climate in function of altitude, with the occurrence of natural vernalization. The propagation is an integral part of the production chain and the first step towards the implantation of the olive tree crop or renovation of the existing orchards. Obtaining quality seedlings, besides guaranteeing uniformity and reliable varietal origin, is a factor that influences the whole life of the orchard,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Seedlings; Cloning; Mini propagation and micro propagation; Cultivation system without soil.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000201004
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An effective method for cloning of partial MADS-box genes related to flower development in groundnut Open Agri
Yuan, M..
Palavras-chave: Genes; PCR; DNA; Cloning; Transcription factors; Genomes; Transcription; Genetic structures; Nucleotides; Molecular cloning.
Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3213
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Animal transgenesis: state of the art and applications. Repositório Alice
MELO, E. O.; CANAVESSI, A. M. O; FRANCO, M. M.; RUMPF, R..
2007
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cloning; Livestock; Animal.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/handle/doc/189124
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Arthropod venom Hyaluronidases: biochemical properties and potential applications in medicine and biotechnology J. Venom. Anim. Toxins incl. Trop. Dis.
Bordon,Karla C F; Wiezel,Gisele A.; Amorim,Fernanda G.; Arantes,Eliane C..
AbstractHyaluronidases are enzymes that mainly degrade hyaluronan, the major glycosaminoglycan of the interstitial matrix. They are involved in several pathological and physiological activities including fertilization, wound healing, embryogenesis, angiogenesis, diffusion of toxins and drugs, metastasis, pneumonia, sepsis, bacteremia, meningitis, inflammation and allergy, among others. Hyaluronidases are widely distributed in nature and the enzymes from mammalian spermatozoa, lysosomes and animal venoms belong to the subclass EC 3.2.1.35. To date, only five three-dimensional structures for arthropod venom hyaluronidases (Apis mellifera and Vespula vulgaris) were determined. Additionally, there are four molecular models for hyaluronidases fromMesobuthus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hyaluronidase; Scorpion; Spider; Caterpillar; Hymenoptera; Insects; Cloning; Heterologous expression; PEGylation; Biotechnological applications.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100214
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Attitudes towards genomic: Research in four Latin American countries Electron. J. Biotechnol.
Rodríguez Yunta,Eduardo; Valdebenito Herrera,Carolina; Misseroni,Adelio; Fernández Milla,Lautaro; Outomuro,Delia; Schiattino Lemus,Irene; Ferrer Lues,Marcela; Lolas Stepke,Fernando.
The present reflection refers to data obtained about the social representations of genomic research and its applications through interviews with legislators and lawyers, biomedical researchers and civilians and the review of scientific and legal literature in four Latin American countries: Argentine, Chile, Mexico and Peru. Several issues are addressed: little access to prevention and therapeutic methods, lack of equity in health benefits, commercialization of gene sequences through patents which leads to commercial exploitation of underdeveloped countries, the possibility of physical or psychological damage or genetic discrimination, the possibility of genetic modifications or abortion for eugenic reasons, the necessity of safeguarding confidentiality,...
Tipo: Journal article Palavras-chave: Cloning; Genome; Social representations; Transgenic.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300003
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Bradyrhizobium japonicumカタラーゼ遺伝子のクローニング方法の確立 OAK
白川, 雪香; 井川, 香子; 佐藤, 哲也; 大和田, 琢二; SHIRAKAWA, Yukika; IGAWA, Keiko; SATO, Tetsuya; OHWADA, Takuji.
Palavras-chave: Bradyrhizobium; カタラーゼ; クローニング; In vitro パッケージング; Bradyrhizobium; Catalase; Cloning; In vitro packaging.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1288
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Caracterização molecular de dois isolados brasileiros de Lettuce mosaic virus apresentando propriedades biológicas distintas Trop. Plant Pathol.
KRAUSE-SAKATE,RENATE; MELLO,RAQUEL N.; PAVAN,MARCELO A.; ZAMBOLIM,EUNIZE M.; CARVALHO,MURILO G.; LE GALL,OLIVIER; ZERBINI,F. MURILO.
Efetuou-se a clonagem e seqüenciamento do gene que codifica a proteína capsidial de dois isolados do vírus do mosaico da alface (Lettuce mosaic virus, LMV) provenientes do estado de São Paulo, previamente caracterizados como pertencentes aos patótipos II (AF198, incapaz de infetar cultivares com os genes de resistência mo1¹ ou mo1²) e IV (AF199, capaz de quebrar a resistência propiciada pelos genes mo1¹ e mo1²), com base na virulência em cultivares diferenciadoras. Análise comparativa das seqüências de nucleotídeos de isolados provenientes da Europa, América do Norte, Oriente Médio e os dois isolados brasileiros não permitiu sua separação em estirpes, pois as porcentagens de homologia foram sempre superiores a 95%. Entretanto, análise filogenética dos...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactuca; Lettuce; Potyvirus; LMV; Capsid protein; Cloning; Resistance.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000200006
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cDNA cloning, Phylogenic Analysis and Gene Expression Pattern of Phenylalanine ammonia-lyase in Sugarcane (Saccharum officinarum L.) BABT
Hashemitabar,Mahmoud; Kolahi,Maryam; Tabandeh,Mohammad Reza; Jonoubi,Parisa; Majd,Ahmad.
The aim of the present study was to clone and characterize a full length cDNA of sugarcane (Saccharum officinarum) phenylalanine ammonia-lyase (SoPAL). Differential tissue expression pattern of the SoPAL transcript and its enzyme activity was also analyzed during the tillering stage of growth. The full-length of SoPAL cDNA was 2118 bp long and contained a protein with 706 amino acids, determined by encoding technique. The amino acid sequence and phylogenic analysis of the cloned SoPAL showed high similarity to PAL from other monocotyledonous such as sorghum (96%), maize (93%) and Bamboos (87.12%). The highest levels of SoPAL transcript were observed in the root and stem, while its minimal gene expression levels were in the leaves and sheath, respectively....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phenylalanine ammonia-lyase; Saccharum officinarumL; Cloning; Tissue expression.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400456
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Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor subunit-1 in the hypothalamus of male sheep R. Bras. Zootec.
Ruiz,Ana Zuley.
The objective of the present study was to report the molecular cloning and determination of the sequences N-methyl-D-aspartate subunit-1 receptor (NMDAR-1) in the hypothalamus of sheep. The re-amplified DNA template for NMDA -1 from the hypothalamus of male sheep was cloned using pBluescript-Sk-plasmids (pBSK, 2958 bp). Purified plasmids containing the NMDA receptor c-DNA were sequenced using the dye-terminator chemistry at the University of Nebraska-Lincoln, Nebraska, USA. Results were entered into the National Center for Biotechnology Web site as accession number AY434689. The sequence of Ovis aries (sheep) NMDAR-1 mRNA from hypothalamus of male sheep has 94-97% homology with Homo sapiens, 94-100% homology with Sus scrofa, and 88-90% homology with Rattus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cloning; NMDA receptor; Ovis aries; Ram; Sequencing.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000400183
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Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae BJMBR
Cui,Yubao; Zhou,Ying; Ma,Guifang; Yang,Li; Wang,Yungang; Shi,Weihong.
Crude extracts of house dust mites are used clinically for diagnosis and immunotherapy of allergic diseases, including bronchial asthma, perennial rhinitis, and atopic dermatitis. However, crude extracts are complexes with non-allergenic antigens and lack effective concentrations of important allergens, resulting in several side effects. Dermatophagoides farinae (Hughes; Acari: Pyroglyphidae) is one of the predominant sources of dust mite allergens, which has more than 30 groups of allergen. The cDNA coding for the group 5 allergen of D. farinae from China was cloned, sequenced and expressed. According to alignment using the VECTOR NTI 9.0 software, there were eight mismatched nucleotides in five cDNA clones resulting in seven incompatible amino acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dermatophagoides farinae (Hughes); Der f 5; Cloning; Expression; Bioinformatics.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800009
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Cloning of putative seed storage protein genes of pearl millet (Pennisetum glaucum). Repositório Alice
CARNEIRO, N. P.; PAIVA, E.; GANDER, E.S.; MARCELLINO, L.H.; PORTUGAL, R.S.; LOPES, M.A..
2000
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Milheto; Clonagem; Semente; Proteina; Armazenamento; Biotecnologia; Pennisetum glaucum; Cloning; Seed; Storage; Protein; Biotechnology.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484463
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Cloning, sequence analysis, and expression of cDNA coding for the major house dust mite allergen, Der f 1, in Escherichia coli BJMBR
Cui,Y.; Zhou,P.; Peng,J.; Peng,M.; Zhou,Y.; Lin,Y.; Liu,L..
Our objective was to clone, express and characterize adult Dermatophagoides farinae group 1 (Der f 1) allergens to further produce recombinant allergens for future clinical applications in order to eliminate side reactions from crude extracts of mites. Based on GenBank data, we designed primers and amplified the cDNA fragment coding for Der f 1 by nested-PCR. After purification and recovery, the cDNA fragment was cloned into the pMD19-T vector. The fragment was then sequenced, subcloned into the plasmid pET28a(+), expressed in Escherichia coli BL21 and identified by Western blotting. The cDNA coding for Der f 1 was cloned, sequenced and expressed successfully. Sequence analysis showed the presence of an open reading frame containing 966 bp that encodes a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dermatophagoides farinae; Der f 1; Recombinant allergens; Cloning; Expression; Bioinformatics.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500006
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Coherent soliton propagation through doped optical fibers: cloning, breakup, and soliton interactions Anais da ABC (AABC)
CAVALCANTI,SOLANGE B.; FONSECA,EDUARDO J. DA S.; CAETANO,DILSON P.; HICKMANN,JANDIR M..
The simultaneous propagation of two optical pulses through a doped nonlinear dispersive medium modelled by a resonant three-level system was investigated numerically, within the framework of a pair of coupled extended nonlinear Schrödinger equations. These included the contribution of the dopant resonances whose dynamics is governed by Bloch equations. In this work, we review the interesting possibilities on the manipulation of fields such as cloning, breakup and soliton interactions, that the combination of coherent population trapping with nonlinear dispersive media offers.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soliton; Quantum coherence; Nonlinearity; Cloning.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652001000200005
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Consumer Preferences for Amount and Type of Fat in Ground Beef AgEcon
Lusk, Jayson L.; Parker, Natalie.
Scientists and beef industry participants are investigating ways to improve the healthiness of beef. We report results of a nationwide mail survey developed to determine consumers’ preferences for fat content in ground beef and identify how consumers would most like to improve the healthiness of beef. The results from a choice-based conjoint experiment indicate that consumers place significant value on reducing saturated fat and the Omega 6:3 ratio in ground beef, but were relatively unconcerned about conjugated linoleic acid. The relatively new method of best-worst scaling was used to further identify which methods consumers most preferred producers use to improve fat content in beef. The results indicate consumers preferred feeding cattle a grass-fed...
Tipo: Journal Article Palavras-chave: Beef; Best-worst scaling; Cloning; Conjoint; Fat; Maximum-difference scaling; Omega 3 fatty acid; Agribusiness; Demand and Price Analysis; Food Consumption/Nutrition/Food Safety; Livestock Production/Industries; Marketing; M31; Q13.
Ano: 2009 URL: http://purl.umn.edu/48763
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Contributo para a caracterização do sistema de lipases em Yarrowia Lipolytica IPB - Escola Superior Agrária
Choupina, Altino.
De entre as espécies de leveduras, Yarrowia lipolytica é uma das maiores produtoras de proteínas extracelulares (proteases ácidas neutras e alcalinas, fosfatase ácida, ribonucleases e lipases). As lipases (triacilglicerol hidrolases) são enzimas importantes no metabolismo das gorduras, catalizando a ruptura dos triacilgliceróis em ácidos gordos livres e glicerol. Para além da baixa solubilidade dos seus substratos naturais, esta hidrólise é catalizada na interface entre um substrato insolúvel e a fase aquosa na qual a enzima está solubilizada. Este aspecto distingue-as das esterases , as quais catalizam preferencialmente a hidrólise de ésteres solúveis em água. As lipases constituem um grupo ubíquo de enzimas capazes de catalizarem diversas reacções,...
Tipo: DoctoralThesis Palavras-chave: Lipase; Yarrowia sp.; Activity; Cloning; YILIP1; YILIP3.
Ano: 2001 URL: http://hdl.handle.net/10198/6187
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Cre-loxP recombination vectors for promoter studies Electron. J. Biotechnol.
Pedersen,Nina; Tuxen Poulsen,Thomas; Skovgaard Poulsen,Hans.
For promoter studies the cloning, subcloning and transfer to different plasmid vectors usually requires use of restriction enzymes and ligation reactions. One obstacle is the nucleotide polymorphisms of eukaryotic genomic DNA, which has the consequence that a sequence often differs from published sequences. Therefore sequencing, rigorous restriction enzyme analysis or introduction of suitable sites has to be performed prior to cloning and subcloning. In addition, conventional methods using restriction enzymes, insert purifications and ligations is expensive and labour demanding. We have developed a fast, efficient and inexpensive Cre recombinase-loxP based method, which allows cloning of promoter regions and subcloning of these into a variety of vectors in...
Tipo: Journal article Palavras-chave: Cloning; Cre recombinase; Plasmid; Reporter genes; Therapeutic genes.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200013
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Different alternatives for producing sprouts of avocado (Persea americana) rootstocks Ciencia e Investigación Agraria
Castro,Mónica; Fassio,Claudia; Darrouy,Nicole; Aedo,Marcela; Jorquera,Lorena.
In Chile, most avocado orchards have been planted using 'Hass' as the scion variety on seedling rootstocks. This has led to the presence of a large variety of individuals, some of which show, among other characteristics, high productivity and a low degree of biennial bearing cycle in the same growing conditions. The most common method used in other countries to propagate such rootstocks from trees involves severe topping; however, this technique is notably destructive, making it difficult to employ in commercial orchards. With the goal of developing alternate methods for forcing shoot production from the rootstock, we tested two different procedures in July 2002 and March 2003, respectively: wounding in conjunction with the application of 1.1 M or 2.2 M...
Tipo: Journal article Palavras-chave: Avocado; Basal sprout; Cloning; Cytokinin; Epicormic sprout; Girdling; Rootstock.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000200007
Registros recuperados: 61
Primeira ... 1234 ... Última
 

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