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Biochemical and morphological analysis of cell death induced by Egyptian cobra (Naja haje) venom on cultured cells J. Venom. Anim. Toxins incl. Trop. Dis.
Omran,M. A. A.; Fabb,S. A.; Dickson,G..
We investigated the in vitro process of cell death caused by Egyptian cobra venom on primary human embryonic kidney (293T) and mouse myoblast (C2C12) cell lines. The aim of these studies was to provide further information about triggering cell death, and suggest methods for eliminating unwanted cells, such as tumour cells. Both cell lines were treated with 10, 20, and 50 m g/ml of Egyptian cobra (Naja haje) venom in serum free media (SFM) and incubated for 8 hours. Total activities of the lactate dehydrogenase (LDH) and creatine kinase (CK) released in the culture during venom incubation were used as an indicator of the venom in vitro cytotoxicity. Cell injury was morphologically recognized and apoptosis determined by a Fluorescing Apoptosis Detection...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cobra venom; Cytotoxicity; Apoptosis.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300004
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Biochemical and toxinological characterization of Naja sumatrana (Equatorial spitting cobra) venom J. Venom. Anim. Toxins incl. Trop. Dis.
Yap,MKK; Tan,NH; Fung,SY.
The lethal and enzymatic activities of venom from Naja sumatrana (Equatorial spitting cobra) were determined and compared to venoms from three other Southeast Asian cobras (Naja sputatrix, Naja siamensis and Naja kaouthia). All four venoms exhibited the common characteristic enzymatic activities of Asiatic cobra venoms: low protease, phosphodiesterase, alkaline phosphomonoesterase and L-amino acid oxidase activities, moderately high acetylcholinesterase and hyaluronidase activities and high phospholipase A2. Fractionation of N. sumatrana venom by Resource® S cation exchange chromatography (GE Healthcare, USA) yielded nine major protein peaks, with all except the acidic protein peak being lethal to mice. Most of the protein peaks exhibit enzymatic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Naja; Cobra venom; Enzyme activity; Cardiotoxin.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400012
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IN VITRO ACTION OF COBRA VENOM ON GOAT SPERMATOZOA ULTRASTRUCTURE BY TRANSMISSION AND SCANNING ELECTRON MICROSCOPY J. Venom. Anim. Toxins
RAHMY,T. R.; AYOUB,M. A..
Goat spermatozoa were incubated in vitro in Tris-citrate buffer, pH 7.2, containing 0, 40, 80, or 160 mug of Naja haje venom/mL buffer for 4 hours. During incubation, the percentages of sperm motility were decreased, while percentages of dead spermatozoa were increased in a time and concentration-dependent manner. The effect of venom concentrations on the ultrastructure of incubated spermatozoa was examined hourly by scanning (SEM) and transmission (TEM) electron microscopy. SEM results showed plasma membrane wrinkling at heads of some spermatozoa after 2 h incubation with 40mug venom. Most spermatozoa suffered membrane wrinkling after 4 h incubation. However, incubation with 80 mug venom caused membrane fractures in most sperm heads after 1 h incubation....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cobra venom; Ultrastructure; Goat spermatozoa; Motility; Naja haje.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100011
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Restoration of adenosine deaminase, histamine, and IgE in organs of mice injected with cobra venom followed by specific treatment and reversal period J. Venom. Anim. Toxins incl. Trop. Dis.
Lipps,B. V..
Adenosine deaminase (ADA), histamine, and IgE are endogenously present in animals. Research from this laboratory reported decreased levels of these substances in organs of mice as a consequence of sub-lethal injection of Naja kaouthia venom. This research reports that decreased ADA, histamine, and IgE levels were prevented by specific treatment and prolonged recovery periods. Adult Balb/c mice injected IM with sub-lethal venom dose were divided into five groups. Group 1 were injected with PBS; Group 2 with anti-cobra venom; and Group 3 with lethal toxin neutralizing factor (LTNF). Groups 4 and 5 were treated with IM or oral synthetic LT-10. After 24 hours, mice were sacrificed and organ homogenates were assayed for ADA, histamine, and IgE. Group 1 showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cobra venom; Mouse organs; ADA; Histamine; IgE; Homeostasis.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200007
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SUB-LETHAL INJECTION OF COBRA VENOM DECREASES ADENOSINE DEAMINASE, HISTAMINE, AND IgE IN ORGANS OF MICE J. Venom. Anim. Toxins
LIPPS,B. V..
The pharmacological substances adenosine deaminase (ADA), histamine, and IgE are endogenously present in animals. They are implicated in allergy and asthma and detectable in blood serum. This research reports the presence of ADA, histamine, and IgE at varying levels in almost all major organs of mice. This research further reports that intramuscular injection of sub-lethal dose of Naja kaouthia venom disrupted homeostasis and lowered the levels of ADA, histamine, and IgE in the organs of mice. Adult Balb/C male mice were injected with a half lethal dose of the venom. The mice were sacrificed at 2, 8, and 24 hours post-injection and different organs were collected. Organs were homogenized, centrifuged, and the supernatants were assayed for ADA, histamine,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cobra venom; Balb/C mice organs; Adenosine deaminase; Histamine; IgE; Naja kaouthia.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100006
Registros recuperados: 5
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