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Lima,G.L.; Santos,E.A.A.; Lima,L.F.; Luz,V.B.; Rodrigues,A.P.R.; Silva,A.R.. |
We compare protocols for the short-term preservation of collared peccarie's ovarian preantral follicles (PFs) by using phosphate buffered saline- (PBS) or powdered coconut water- (ACP(r)) based medium. For morphology analysis each pair of ovaries collected from six females was divided into nine fragments. One fragment was destined for morphology analysis (histology and transmission electron microscopy - TEM), constituting the control group and the other fragments were placed in tubes with PBS or ACP(r), packed in 5 L Styrofoam boxes, stored for 4h, 12h, 24h, and 36h, and then analyzed. For viability analysis a pair of ovaries from two additional females was divided into nine fragments; one fragment was immediately destined for viability analysis (Trypan... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Collared peccaries; Preantral follicles; Coconut water; Ovary; Oocytes. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601623 |
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