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Registros recuperados: 16
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A new surgical technique to treat corneal perforations using amniotic membrane and surgical adhesive Arq. Bras. Med. Vet. Zootec.
Pontes,K.C.S.; Borges,A.P.B.; Eleotério,R.B.; Frazão,A.C.N.; Machado,D.P.D.; Reis,E.C.C.; Sepúlveda,R.V.; Duarte,T.S.; Laus,J.L..
The objectives of this study were to compare surgical techniques and the effects of using n-butyl 2-cyanoacrylate and bovine amniotic membrane to repair perforated lesions in corneas. Penetrating keratoplasty was performed in sixty New Zealand White rabbits under general anesthesia. Group 1 (G1) was treated with n-butyl 2-cyanoacrylate, group 2 (G2) received a fragment of amniotic membrane through the anterior chamber and application of n-butyl 2-cyanoacrylate over the lesion, group 3 (G3) was treated with the same technique as G2 with the addition of an amniotic membrane bandage covering the cornea and sutured in the limbus region, and group 4 (G4) was treated with an amniotic membrane sutured to the lesion and an amniotic membrane bandage sutured in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rabbits; Cornea; Cyanoacrylate; Glycerin; Ophthalmic surgery.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300655
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Bilateral lamellar keratoplasty in descemetocele treatment in dog with botulism by use of equine renal capsule and conjunctival pedicle graft Ciência Rural
Laus,José Luiz; Galera,Paula Diniz; Schocken-Iturrino,Ruben Pablo; Cavassani,Marluce de Macedo; Andrade,Alexandre Lima de.
A 3-year-old, male mixed breed dog with botulism and bilateral descemetocele was submitted to lamellar keratoplasty with equine renal capsule preserved in glycerin in the right eye and conjunctival pedicle graft in the left eye. The evolution was satisfactory in both eyes, but better in the eye receiving the equine renal capsule, because the corneal transparence was more evident in that eye. On the other hand, the surgical period was more quickly in the eye receiving the equine renal capsule because the preparation of the conjunctival pedicle before the keratoplasty was not necessary.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Keratoplasty; Cornea; Descemetocele; Botulism.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781999000200029
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Collagens and proteoglycans of the corneal extracellular matrix BJMBR
Michelacci,Y.M..
The cornea is a curved and transparent structure that provides the initial focusing of a light image into the eye. It consists of a central stroma that constitutes 90% of the corneal depth, covered anteriorly with epithelium and posteriorly with endothelium. Its transparency is the result of the regular spacing of collagen fibers with remarkably uniform diameter and interfibrillar space. Corneal collagen is composed of heterotypic fibrils consisting of type I and type V collagen molecules. The cornea also contains unusually high amounts of type VI collagen, which form microfibrillar structures, FACIT collagens (XII and XIV), and other nonfibrillar collagens (XIII and XVIII). FACIT collagens and other molecules, such as leucine-rich repeat proteoglycans,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cornea; Proteoglycan; Glycosaminoglycan; Collagen; Dermatan sulfate; Keratan sulfate.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800009
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Dose-dependent in vitro inhibition of rabbit corneal matrix metalloproteinases by an extract of Pothomorphe umbellata after alkali injury BJMBR
Barros,L.F.M.; Barros,P.S.M.; Röpke,C.D.; Silva,V.V.; Sawada,T.C.H.; Barros,S.B.M.; Belfort Jr.,R..
The in vitro ability of Pothomorphe umbellata ethanolic crude extract to inhibit matrix metalloproteinase (MMP) in normal cornea and in cornea after alkali injury was demonstrated. Corneas of albino rabbits were injured with 1 N NaOH for 20 s. After 48 h the corneas were excised, homogenized and analyzed for MMP-9 (92 kDa), pro-MMP-2 (72 kDa) and MMP-2 (67 kDa) activity by gelatin zymography. The activity was also measured in untreated corneas. After electrophoresis of 20 µg protein, gels were incubated with 50, 100, or 250 µg/mL lyophilized hydroethanolic (1:1) root crude extract of P. umbellata standardized for 4-nerolidylcatechol (7.09%). The activity of the enzymes was compared with that of untreated gel. At 48 h after injury, the activity of all MMPs...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cornea; Alkali injury; Metalloproteinases; Pothomorphe umbellata.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800014
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Effects of ethyl-cyanoacrylate and octyl-cyanoacrylate on experimental corneal lesions in rabbits Arq. Bras. Med. Vet. Zootec.
Barbosa,V.T.; Thiesen,R; Soares,E.G.; Machado,M.R.F.; Laus,J.L..
The use of ethyl-cyanoacrylate and octyl-cyanoacrylate were clinically and histopathologically compared on the corneas of 36 rabbits after lamellar keratectomy (standardized diameter and depth). The animals were distributed into two groups, one for each type of adhesive. From each group, six subgroups were histopathologically evaluated on the 3rd, 7th, 14th, 21st, 30th, and 60th day post-operative. General (daily) and ophthalmic (days 0, 1, 3, 5, 7, 14, 21, 30, 44, and 60) evaluations clinically indicated that there were significant differences for the variables water intake, attitude, blepharitis, corneal edema, and fluorescein test. The adhesive permanence time for octyl-cyanoacrylate (17.22 days) was greater than that for ethyl-cyanoacrylate (7.66...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rabbit; Cornea; Cyanoacrylate.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000500009
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Effects of topical 2% cyclosporine A on the corneas of dogs subjected to lamellar keratoplasty with a graft of equine pericardium preserved in glycerin: clinical and morphological evaluation Ciência Rural
Teixeira,Roseli Borges; Barros,Paulo Sergio de Moraes; Martins,Maria Cristina.
The effects of topical 2% cyclosporine A on the cornea of dogs subjected to experimental lamellar keratoplasty with an equine pericardial graft were evaluated. Ten dogs were grouped to be evaluated 3, 7, 15, 30 and 60 days after surgery. Animals received bilateral grafts followed by the application of a 2% cyclosporine A ointment on the left eye (treated eye) and the ointment base on the right eye (control eye) twice a day. The ophthalmic evaluation showed profound bilateral blepharospasm, photophobia and a mucous secretion until the 7th day after surgery; corneal vascularization starting in the limbus was observed as early as the 3rd day in both eyes; opacification and vascularization were more intense in the treated cornea on days 15 and 30 after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyclosporine; Cornea; Pericardium; Vascularization; Keratoplasty; Dogs.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000100020
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Excision of sclerocorneal limbus in dogs and resulting clinical events: study of an experimental model Arq. Bras. Med. Vet. Zootec.
Brunelli,A.T.J.; Vicenti,F.A.M.; Oriá,A.; Campos,C.F.; Doria Neto,F.A.; Laus,J.L..
The consequences of complete removal of the sclerocorneal limbus epithelium were evaluated, as well as the model for such, in which n-heptanol and superficial keratectomy were used. The association of n-heptanol and keratectomy allowed a complete excision of stem cells. Moreover, the destruction of these cells led to significant tectonic alterations that could be employed as a feasible model for clinical experimentation of corneal transplantation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Stem cell; Cornea.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000100009
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Experimental lamellar corneal graft in dogs using preserved equine pericardium Braz. J. Vet. Res. Anim. Sci.
BARROS,Paulo Sergio de Moraes; SAFATLE,Angélica Mendonça de Vaz; RIGUEIRO,Moacyr.
The purpose of this paper was to study the preserved equine pericardium as a corneal lamellar graft of the dog. We performed in twelve adults, male and female mongrel dogs a superficial keratotomy 5 mm square and a fragment of equine pericardium of the same shape was sutured in place with multiple 7-0 nylon simple interrupted sutures. Each five animals were sacrificed in days 7, 15, 30 and 100 after surgery. The clinical and histological exams permitted us to assert that this membrane is a useful tissue to be used as a corneal lamellar graft of the dog.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cornea; Grafts; Pericardium.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000600005
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Lamellar keratoplasty in rabbits using an allogeneic free omental graft and omentum-derived mesenchymal cells associated with the canine amniotic membrane Ciência Rural
Barros,Séfora Vieira da Silva Gouvêa de; Aldrovani,Marcela; Lacerda,Luciana Cenço Correa de; Horr,Mônica; Marinho,Fábio Andrade; Lima,Tiago Barbalho; Silveira,Camila Pinho Balthazar da; Brito,Fábio Luiz da Cunha; Bassaneze,Vinícius; Flecher,Mayra Cunha; Beltrame,Renato Travassos; Nakamuta,Juliana Sanajotti; Krieger,José Eduardo; Laus,José Luiz; Oliveira,Krishna Duro de.
ABSTRACT: The objective of this research was to evaluate the clinical and microscopic effects in rabbits of lamellar keratoplasty using allogeneic omentum associated with canine amniotic membrane (AM). Rabbits were divided into two groups: one received the allogeneic free omental graft covered with the AM (OM-graft group), while the other received the AM graft containing omental mesenchymal cells (OM-cell group). Clinical signs were evaluated on different postoperative days. After the clinical assessments, the rabbits were euthanized and their corneas were obtained for histopathology and immunohistochemistry (Ki-67, marker for proliferation). Both groups showed chemosis, blepharospasm, eye discharge, hyperemia, and corneal opacity/edema. Neovascularization...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amniotic membrane; Cornea; Omentum; Progenitor cells; Wound healing..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001001838
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Limbal autograft transplantation in a dog with alkali-induced ulceration Ciência Rural
Brito,Fábio Luiz da Cunha; Cunha,Olicies da; Laus,José Luiz.
A case of a 3-month-old female mongrel dog with a history of apathy and previous contact with an alkaline (sodium hydroxide) is reported. The dog was reluctant to open the right eye. Ophthalmic examination revealed blepharospasm, photophobia, epiphora, discrete chemosis, conjunctival hyperemia, and diffuse corneal edema involving the limbus. The fluorescein test was positive and the result of the Schirmer tear test was 32mm min-1. On the basis of these findings the diagnosis was alkali-induced ulceration and limbal autograft transplantation was performed. Corneal vascularization was observed by the third postoperative day, with intensification in vessel number and caliber on subsequent days and small areas of corneal transparency. The present results show...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cornea; Ulcerative keratitis; Sodium hydroxide; Transplant.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000800028
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Maintenance of the corneal epithelium is carried out by germinative cells of its basal stratum and not by presumed stem cells of the limbus BJMBR
Haddad,A.; Faria-e-Sousa,S.J..
The purpose of this investigation was to analyze the proliferative behavior of rabbit corneal epithelium and establish if any particular region was preferentially involved in epithelial maintenance. [3H]-thymidine was injected intravitreally into both normal eyes and eyes with partially scraped corneal epithelium. Semithin sections of the anterior segment were evaluated by quantitative autoradiography. Segments with active replication (on) and those with no cell division (off) were intermingled in all regions of the tissue, suggesting that the renewal of the epithelial surface of the cornea followed an on/off alternating pattern. In the limbus, heavy labeling of the outermost layers was observed, coupled with a few or no labeled nuclei in the basal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cornea; Epithelium; Autoradiography; Germinative cells; Stem cells; Limbus.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600470
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Morphology of endothelial cells from different regions of the cornea of dogs Ciência Rural
Hünning,Paula Stieven; Andrade,Maria Cristina Caldart de; Carissimi,André; Pigatto,João.
ABSTRACT: The aim of this study was to evaluate the morphology of endothelial cells from different areas of the cornea of dogs. Twenty healthy eyes from 10 dogs, females or males, of different ages were studied. Corneal endothelium morphology of superior, inferior, central, nasal and temporal areas was assessed by 0.2% alizarin red staining using an optic microscope. One hundred endothelial cells from each corneal area were analyzed. In all areas of the cornea studied were found endothelial cells with four sides, five sides, six sides and seven sides. There was no significant difference regarding endothelial cell morphology in all corneal regions evaluated. Thus, the morphology of the central cornea area represents the entire endothelial mosaic and may be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Cornea; Endothelium; Alizarin red; Pleomorphism.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000605
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The effects of the subconjunctival injection of bevacizumab (Avastin®) on angiogenesis in the rat cornea Anais da ABC (AABC)
Barros,Luiz F.M.; Belfort-Jr,Rubens.
The purpose of this study was to evaluate the effects of the use of the subconjunctival injection of bevacizumab (Avastin®) on angiogenesis in the rat cornea. Corneas of 20 Wistar male rats were cauterized with silver nitrate crystal. Animals were divided in four groups: control group (GC) that received subconjunctivally 0.02 ml of 0.9% saline solution on the day of the lesion; group GO that received subconjunctivally 0.02 ml of bevacizumab just after the lesion; group G3 that received bevacizumab on day 3 and group G5 that received bevacizumab on day 5 after lesion. Animals were euthanized on day 7. The newly formed vessels were quantified after China Ink perfusion and photographs were obtained and analyzed in a computerized system (Image Pro-Plus®). In...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bevacizumab; Cornea; Neovascularization; Angiogenesis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000300004
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Topical 1% Nalbuphine on corneal sensivity and epitheilization after experimental lamellar keratectomy in rabbits Ciência Rural
Silva,Miguel Ladino; Piso,Dunia Yisela Trujillo; Ribeiro,Alexandre Pinto; Laus,Jose Luiz.
The present study was aimed to evaluate the effects of topical 1% nalbuphine on corneal sensitivity and re-epithelialization, after lamellar keratectomy in rabbits. All protocols were approved by the Animal Care Comission of São Paulo State University (Protocol 028793-08) and were conducted in accordance with the Institutional Animal Committee and the Association for Research in Vision and Ophthalmology (ARVO) statement for the use of animals in research. Surgeries were performed on the left eye (Nalbuphine Group) and on the right eye (Control Group). Two groups were formed (n=10) and corneas received either 30µl of 1% nalbuphine (NG) or 30µl of 0,9% saline (CG). Treatments occurred at 7, 11, 15 and 19 hours. After the surgery, the corneas were stained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rabbit; Cornea; Re-epithelialization; Nalbuphine; Pain.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000400017
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Use of fibrin glue derived from snake venom in the repair of deep corneal ulcers: experimental study in dogs (Canis familiaris, Linnaeus, 1758) J. Venom. Anim. Toxins incl. Trop. Dis.
Sampaio,R. L.; Ranzani,J. J. T.; Brandão,C. V. S.; Thomazini-Santos,I. A.; Barraviera,B.; Barraviera,S. R. C. S.; Giannini,M. J. S. M..
Fibrin glue has been researched as an alternative method for tissue synthesis and is known for its capability to promote hemostasis at the application site, good approximation of wound edges and fast healing. The current study consisted in the application of fibrin glue derived from snake venom as treatment for experimental corneal ulcers. Twenty-one dogs had their corneas experimentally prepared through lamellar keratectomy (of standardized diameter and depth). Animals were divided into seven groups of three animals each. Six experimental groups were periodically evaluated and collection was carried out on the 1st, 3rd, 7th, 15th, 30th and 60th post-operative days, whereas one control group was evaluated throughout the experiment. Analyses consisted in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fibrin glue; Snake venom; Cornea; Corneal ulcer; Keratoplasty.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400014
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Use of fresh autogenous vaginal tunic in the experimental lamellar keratoplasty in dogs (Canis familiaris, LINNAEUS, 1758) Braz. J. Vet. Res. Anim. Sci.
GALERA,Paula Diniz; LAUS,José Luiz; FERREIRA,Affonso Luiz.
Autogenous vaginal tunics have been researched in lamellar keratoplasty in dogs using fresh autogenous grafts in the repair of superficial keratectomies. Fourteen dogs have been used for the evaluation at the early, intermediary and late postoperative periods. Photophobia, blepharospasm, ocular discharge, edema, neovascularization and pigmentation have been observed. The results have showed that the original procedure is useful for the repair of corneas after superficial keratectomies.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cornea; Keratoplasty; Tunica vaginalis; Dogs.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000600010
Registros recuperados: 16
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