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A comparative field study of growth, survival and reproduction of Crassostrea gigas, C-angulata and their hybrids ArchiMer
Soletchnik, Patrick; Huvet, Arnaud; Le Moine, Olivier; Razet, Daniel; Geairon, Philippe; Faury, Nicole; Goulletquer, Philippe; Boudry, Pierre.
This paper reports a comparative study of two cupped oysters, Crassostrea gigas and C angulata, and their hybrids reared in the Marennes-Oleron Sound (France) under usual farming conditions. The relative proficiency of these species was characterized in terms of growth, survival and reproduction, three major concerns for oyster farming. Four populations of each taxon were sampled (C gigas: Japan and France; C angulata: Taiwan and Spain) and used as progenitors to produce six different progenies under common hatchery and nursery conditions (transfer of 5 to 6 g of seed oysters at sea and rearing in bags for 2 years). Mortality profiles differed greatly among the progenies. The C angulata progeny of Taiwanese origin gave the poorest results, with 100%...
Tipo: Text Palavras-chave: Atlantic coast; Crassostrea gigas; Phenotypes; Hybrids; Oyster fanning.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-560.pdf
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A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments ArchiMer
Mccombie, Helen; Ledu, Christophe; Phelipot, Pascal; Lapegue, Sylvie; Boudry, Pierre; Gerard, Andre.
We present a new method to produce tetraploid Crassostrea gigas by cytochalasin B inhibition of polar body 2 expulsion in diploid females crossed with tetraploid males. This offers a means of direct introgression of genetic characters from selected diploid to tetraploid lines, avoiding a triploid step. Offspring larval ploidy shifted over time and depended on size, with tetraploids more frequent among the smaller larvae and triploids among the large. Viable tetraploids were found at 4 and 6 months, indicating the technique was successful. The possibility that gynogenesis occurred was tested by microsatellite analysis to confirm the presence of paternally inherited alleles. These were present in all animals of the 2n x 4n + CB (female first) cross. However,...
Tipo: Text Palavras-chave: Oyster breeding; Gynogenesis; Polar body; Cytochalasin B; Polyploidy; Crassostrea gigas.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-632.pdf
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A Dynamic Energy Budget (DEB) growth model for Pacific oyster larvae, Crassostrea gigas ArchiMer
Rico-villa, Benjamin; Bernard, Ismael; Robert, Rene; Pouvreau, Stephane.
Tipo: Text Palavras-chave: Dynamic Energy Budget; Crassostrea gigas; Bivalve larvae; Ecophysiology; Growth.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00009/11996/9040.pdf
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A flow cytometric approach to study intracellular-free Ca2+ in Crassostrea gigas haemocytes ArchiMer
Aton, Emilie; Renault, Tristan; Gagnaire, Beatrice; Thomas Guyon, Hélène; Cognard, C; Imbert, N.
Bivalve haemocytes are essential in defence mechanisms including phagocytosis. They also produce molecules including hydrolytic enzymes and antimicrobial peptides that contribute to pathogen destruction. Although haemocyte activities have been extensively studied, relatively little is known about the intracellular signalling pathways that are evoked during haemocyte activation and especially the role of calcium. Flow cytometry has been used for the first time to define the effect of cell incubation in haemolymph and artificial sea water (ASW) on Pacific oyster, Crassostrea gigas, haemocytes. Cell viability, enzymatic activities (esterases and amino peptidases), phagocytosis and granulocyte percentage were analysed. Viability and some activities were...
Tipo: Text Palavras-chave: Cellular activities; Fluo 3/AM; Calcium; Flow cytometry; Haemocytes; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-935.pdf
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A flow-through rearing system for ecophysiological studies of Pacific oyster Crassostrea gigas larvae ArchiMer
Rico Villa, Benjamin; Woerther, Patrice; Mingant, Christian; Le Piver, David; Pouvreau, Stephane; Hamon, Michel; Robert, Rene.
While literature is relatively abundant on adult shellfish, ecophysiological studies at the larval stage are scarce because of both technical difficulties and inadequate methodology. A tool dedicated to provide basic information for larval ecophysiology was accordingly developed. Two steps were followed: first a flow-through method of Crassostrea gigas larval culture was perfected during a set of experiments in which rearing systems and larval density were assessed. Then a continuous hydrobiological data recorder was adapted with modifications to comply with our experimental aim. SILO (Système d'Instrumentation des Larves en flux Ouvert) allowed the successive acquisition of hydrobiological parameters in ten 150 l larval tanks in which larvae were reared...
Tipo: Text Palavras-chave: Temperature; Ecophysiology; Bivalve larvae; Crassostrea gigas; Flow through.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4167.pdf
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A Functional Study of Transforming Growth Factor-Beta from the Gonad of Pacific Oyster Crassostrea gigas ArchiMer
Corporeau, Charlotte; Groisillier, Agnes; Jeudy, Alexandra; Barbeyron, Tristan; Fleury, Elodie; Fabioux, Caroline; Czjzek, Mirjam; Huvet, Arnaud.
The transforming growth factor (TGF)-beta superfamily is a group of important growth factors involved in multiple processes such as differentiation, cell proliferation, apoptosis and cellular growth. In the Pacific oyster Crassostrea gigas, the oyster gonadal (og) TGF-beta gene was recently characterized through genome-wide expression profiling of oyster lines selected to be resistant or susceptible to summer mortality. Og TGF-beta appeared specifically expressed in the gonad to reach a maximum when gonads are fully mature, which singularly contrasts with the pleiotropic roles commonly ascribed to most TGF-beta family members. The function of og TGF-beta protein in oysters is unknown, and defining its role remains challenging. In this study, we develop a...
Tipo: Text Palavras-chave: Transforming growth factor-beta; Bacterial expression; In vivo antibody inhibition; Reproduction; Crassostrea gigas; Marine bivalve.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00042/15371/13438.pdf
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A "G" chromosome banding study of three cupped oyster species : Crassostrea gigas, Crassostrea angulata and Crassostrea virginica (Mollusca : Bivalvia) ArchiMer
Leitao, Alexandra; Thiriot-quievreux, Catherine; Malheiro, Isabel; Boudry, Pierre.
The G-banding technique was performed on chromosomes from gill tissue of three cupped oyster species : Crassostrea gigas, Crassostrea angulata and Crassostrea virginica. Identification of the ten individual chromosome pairs was obtained. Comparative analysis of G-banded karyotypes of the three species showed that their banding patterns generally resembled each other, with chromosome pair 3 being similar in all three species. However, differences from one species to another were also observed. The G-banding pattern highlighted greater similarities between C. gigas and C. angulata than between these two species and C. virginica, thus providing an additional argument for genetic divergence between, these two evolutionary lineages. C. gigas and C. angulata...
Tipo: Text Palavras-chave: Crassostrea virginica; Crassostrea angulata; Crassostrea gigas; G banding; Chromosome.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-2736.pdf
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A la recherche de l'huître portuguaise: identification de marqueurs moléculaires discriminant Crassostrea angulata et Crassostrea gigas ArchiMer
Cornette, Florence.
L'étude historique de l'ostréiculture française monte que cette activité est caractérisée par des périodes de prospérités et de pénurie. Des épizooties ont été à l'origine de ces alternances. A chacune de ces période, on a pu constater des changements d'espèces : Ostrea edulis fut remplacée par Crassostrea angulata, de même celle-ci fut remplacée par Crassostrea gigas dans les années 1970. L'étude de la bibliographie montre que les techniques utilisées pour distinguer des espèces Crassostrea angulata et Crassostrea gigas sont insuffisantes. L'évolution des technologies en matière d'analyses génétiques ouvrent de nouvelles perspectives. La recherche de marqueurs moléculaires réalisée sur Crassostrea angulata et Crassostrea gigas, par amplification par PCR...
Tipo: Text Palavras-chave: Crassostrea angulata; Crassostrea gigas; Marqueurs moléculaires; ADN mitochondrial; PCR; Enzymes de restrictions; Haplotypes.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00049/15979/13421.pdf
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A latitudinal gradient of seasonal temperature variation recorded in oyster shells from the coastal waters of France and The Netherlands ArchiMer
Lartaud, Franck; Emmanuel, Laurent; De Rafelis, Marc; Ropert, Michel; Labourdette, Nathalie; Richardson, Christopher A.; Renard, Maurice.
Cathodoluminescence (CL) microscopy of the foliated calcite shell hinge sections of live-collected oyster Crassostrea gigas collected at seven locations along a latitudinal gradient from the Netherlands in the North Sea to the Atlantic coast of France, revealed variations in luminescence that were attributable to seasonal variations in calcification of the hinge. Photomicrographs of hinge sections and luminescence profiles were analyzed to define a micro-sampling strategy that was adopted to drill the hinge samples to determine their isotopic composition. Reconstructed seasonal seawater temperatures determined from the stable oxygen isotope (delta O-18) composition along growth profiles from 32 oyster shell hinges showed distinct seasonal isotopic cycles...
Tipo: Text Palavras-chave: Seasonality; Palaeotemperatures; Stable isotopes; Cathodoluminescence; Crassostrea gigas; Mollusk shells.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/2010/publication-7296.pdf
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A literature review as an aid to identify strategies for mitigating ostreid herpesvirus 1 in Crassostrea gigas hatchery and nursery systems ArchiMer
Rodgers, Chris; Arzul, Isabelle; Carrasco, Noèlia; Furones Nozal, Dolores.
An understanding of husbandry strategies and any associated risk factors is important for designing management control measures that can reduce mortality in Pacific oysters, Crassostrea gigas, caused by ostreid  herpesvirus 1 (OsHV-1). The type of culture facility can be considered in relation to the potential pathways that could lead to the entry of a pathogen and its survival. In addition, the animal host (e.g. age, physiological state,  selective breeding programmes), husbandry procedures (e.g. stocking density), the pathogen itself (e.g. pathogenicity, virulence) and environmental effects (e.g. temperature) represent other relevant interconnected factors. However, all these factors provide valuable background information for outlining the mitigation...
Tipo: Text Palavras-chave: Crassostrea gigas; Husbandry; Management strategies; Ostreid herpesvirus 1; Oyster herpesvirus; Risk factors.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00474/58581/61146.pdf
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A methology for the stocks assessments of cultivated oysters among along the French Atlantic coasts ArchiMer
Bacher, Cedric; Baud, Jean-pierre; Bodoy, Alain; Deslous-paoli, Jean-marc; Dreno, Jean-paul; Heral, Maurice; Maurer, Daniele; Prou, Jean.
An assessment of the reared stocks of the Pacific oyster (Crassostrea gigas ) has been recently conducted on the major French areas of molluscs culture. The methodology proposed included both estimates of densities obtained by subsampling in the field, and aerial photographs which covered the cultivated, intertidal area. The size and the number of samples were designed to reach a precision of 10% on the final assessment in each area. The field work consisted in weighing and counting the oyster in unit areas of 0.5 m2 for the bottom culture or in trays or bags, for the off bottom technique. These results give a better knowledge of the technique evolution and of the general structures in the cultivated areas.
Tipo: Text Palavras-chave: Modeling; Estimation; Stocks; Crassostrea gigas; Oysters.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/acte-2864.pdf
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A rating system for oyster purification. Hazard analysis critical control point (HACCP) application to purification of live oysters ArchiMer
Bird, P.
A Critical Hazard Analysis Rating (CHAR) based on the Hazard Analysis Critical Control Point (HACCP) concept is used to monitor oyster purification in New South Wales (N.S.W.), Australia. Critical areas for assessment include oyster condition, separation of raw and treated oysters, water quality (turbidity, salinity, temperature, aeration, circulation), purification period, flow rate, steriliser efficiency and tank hydraulics. Batch records and product identification are included. Purification supplements a total management program for producing safe oysters. The rating can also be used to endorse oysters produced under industry self-regulatory, quality assurance programs. The four main CHAR areas are operational standards, quality assurance, public health...
Tipo: Text Palavras-chave: Saccostrea commercialis; Crassostrea gigas; Bivalvia; Marine molluscs; Safety regulations; Public health; Self purification.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1607.pdf
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A single bio-energetics growth and reproduction model for the oyster Crassostrea gigas in six Atlantic ecosystems ArchiMer
Alunno-bruscia, Marianne; Bourles, Yves; Maurer, Daniele; Robert, Stephane; Mazurie, Joseph; Gangnery, Aline; Goulletquer, Philippe; Pouvreau, Stephane.
Many studies based on bioenergetics growth models have investigated the effects of environmental factors on oyster (Crassostrea gigas) growth and physiology. However, most of these models are site-specific and cannot be applied to other culture sites without the re-estimation of parameters or re-formulation of some processes. We aimed to develop a generic growth model suitable for application in contrasting environments, with a constant set of parameters. We tested the oyster-DEB model (Bourlès et al. 2009) for the stimulation of C. gigas growth in different cohorts (spats and adults) at major shellfish culture sites in France, in several years: Arcachon (1993–1994); Marennes-Oléron (2007); Quiberon (1999, 2000, 2001); Rade de Brest (2008); Baie du...
Tipo: Text Palavras-chave: DEB theory; Modelling; Bivalves; Crassostrea gigas; Phytoplankton; Temperature effect; Coastal environment..
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00043/15403/12762.pdf
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A study of autophagy in hemocytes of the Pacific oyster, Crassostrea gigas ArchiMer
Picot, Sandy; Morga, Benjamin; Faury, Nicole; Chollet, Bruno; Dégremont, Lionel; Travers, Marie-agnes; Renault, Tristan; Arzul, Isabelle.
Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the...
Tipo: Text Palavras-chave: Autophagosome; Autophagy; Crassostrea gigas; Flow cytometry; Fluorescence microscopy; Hemocytes; Transmission electron microscopy.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00487/59905/63087.pdf
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Absence de captage de Crassostrea gigas dans le bassin de Marennes-Oléron en 1981 : causes et conséquences ArchiMer
Berthome, Jean-paul; Deslous-paoli, Jean-marc; Heral, Maurice; Razet, Daniel; Garnier, Jacqueline.
The want of C. gigas spat-catching in the Marennes-oleron basin in 1981 can be due to various different reasons which are analysed here. Physico -chemical and trophic factors that can induce variations in the quality of gametogenesis, are successively in comparison with the year 1979 when reproduction was good. The biochemical evolution and a study of gonads slow a lack in maturation in 1981 even during the summer period. Late maturation of gonads bring about physiological consequences which induced mortality of cultivated oysters at the end of winter. A feeble recruit and a decrease of the cultivated stock have economic consequences which here sketched up.
Tipo: Text Palavras-chave: Larvae; Recrutment; Spat; Crassostrea gigas; Oysters; Larves; Recrutement; Captage; Crassostrea gigas; Huîtres.
Ano: 1982 URL: http://archimer.ifremer.fr/doc/1982/acte-2948.PDF
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Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridation et conservatoire de souches. Amélioration de la qualité chez l'huîtres creuses Crassostrea gigas par sélection de souches performantes - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Convention 97 RPC-R-54 "Génétique" - Rapport année 1997 ArchiMer
Boudry, Pierre; Lapegue, Sylvie; Goyard, Emmanuel; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
Les objectifs pour l'année 1997 étaient les suivants : • Poursuivre les premières reproductions des espèces pour lesquelles nous disposons de géniteurs GO. L'objectif est toujours de limiter le stockage d'animaux importés en produisant une descendance simultanément à des témoins C. gigas qui serviront de comparaison. • Tenter des hybridations inter-spécifiques des différents taxons présents au sein du conservatoire en fonction des connaissances disponibles dans la littérature sur ce sujet (voir pour revue Gaffney et Allen, 1993). • Importer de nouvelles souches ou espèces. Élargir la gamme des espèces présentes au sein du conservatoire de souches (les capacités d'accueil de la salle de quarantaine étant limitée).
Tipo: Text Palavras-chave: Génétique; Génétique des Populations; Acclimatation; Hybridation; Marqueurs génétiques; Sélection génétique; Crassostrea gigas.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00044/15519/12906.pdf
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Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea : hybridation et conservatoire de souches. Contrat Région Poitou-Charentes 1999 - Convention 99 RPC-A-203 "Génétique" ArchiMer
Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
Dans le cadre du plan Etat-Région 1994-1998, un programme de conservatoire de souches a été initié au Laboratoire IFREMER de La Tremblade. Ce conservatoire a pour objectif d'acclimater différentes espèces ou souches d'huîtres creuses (genres Crassostrea et Saccostrea) afin d'acquérir des connaissances sur ces espèces et d'évaluer l'intérêt qu'elles pourraient présenter pour l'ostréiculture locale. L'importation d'espèces étrangères sur les côtes françaises est désormais strictement réglementée: la dissémination d'une nouvelle espèce peut se révéler néfaste d'un point de vue écologique et nuire aux productions existantes. De plus, ces huîtres peuvent être porteuses de pathogènes qui, s'il venaient à se répandre dans le milieu, pourraient toucher les stocks...
Tipo: Text Palavras-chave: Génétique; Acclimatation; Croisement; Crassostrea gigas; Crassostrea ariakensis; Crassostrea sikamea; Hybridation.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00044/15514/12901.pdf
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Acquisition de donnée en continu sur la matière particulaire de la Baie estuarienne de Marennes-Oléron pendant le bloom printaniter planctonique : effet sur le taux de filtration de l'huître Crassostrea gigas ArchiMer
Heral, Maurice; Razet, Daniel; Prou, Jean.
The continuous obtention of different signals has been achieved for 9 days at a -fixed station during May 1988. The speed and direction of the currents have been monitoring concurrently with "in situ" turbidity and fluorescence in relation with the meteorological conditions. The concentration in particulate matter and the planktonic fluorescence fluctuated in direct relation with the water depth, the speed of the current and with the origin of the water masses. The oysters submitted to these large fluctuated conditions showed not constant filtration rate. The filtration calculated by turbidimeters (2 1 h-l g-1 dry weight) was three time less than the one achieved with fluorimeters (61 h-l g-1 dry weight). The filtration rate were fluctuated but not in...
Tipo: Text Palavras-chave: France; Marennes Oleron bay; Acquirring of continous data; Filtration; Crassostrea gigas; Particular matter; Marennes Oléron; Aquisition données; Courantologie; Filtration; Crassostrea gigas; Matière particulaire.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-3021.pdf
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Action de l'acétate de tributyle-étain sur les oeufs et les larves D de deux mollusques d'intérêt commercial : Crassostrea gigas (Thunberg) et Mytilus galloprovincialis (Lmk) ArchiMer
Robert, Rene; His, Edouard.
The Tributyl tin acetate, a component of certain antifouling paints, has deleterious effects on eggs and D shaped larvae from Crassostrea gigas at 5 p.p.b. (HIS & ROBERT, 1980). Here we have tested concentrations as low as 1 p.p.b. on eggs and on 24 hours old larvae both from Crassostrea gigas and Mytilus galloprovincialis. The same results are got ; this product kills embryos and larvae in a few days ; it prevents growth of 24 hours old larvae exposed to this toxic. Mussels larvae seem to be less sensitive.
Tipo: Text Palavras-chave: Antifouling; Larves; Pollution; Toxicité; Bivalvia; Crassostrea gigas; Mytilus galloprovincialis; Antifouling; Larvae; Pollution; Toxicity; Bivalvia; Crassostrea gigas; Mytilus galloprovincialis.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/00037/14862/12185.pdf
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Action d'un sel organo-métallique, l'acétate de tributyle-étain sur les oeufs et les larves D de Crassostrea gigas (Thunberg) ArchiMer
His, Edouard; Robert, Rene.
L'action de l'acétate de tributyle-étain, à des concentrations variant de 5 à 100 micro- grammes/l a été étudiée sur les oeufs de Crassostrea gigas en cours de fécondation. Parallèlement les mêmes concentrations ont été testées sur les larves D âgées de 24 heures.
Tipo: Text Palavras-chave: Huître; Crassostrea gigas; Expérimentation; Larves; Reproduction; Sel organo-métallique; Acétate de tributyle étain.
Ano: 1980 URL: http://archimer.ifremer.fr/doc/00009/11983/8687.pdf
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