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Assessing the viability of cryopreserved coconut zygotic embryos by electrolytic conductivity and potassium leaching PAB
Gomes-Copeland,Kicia Karinne Pereira; Lédo,Ana da Silva; Almeida,Fabrício Tavares Cunha de; Miranda,Roberta Pereira; Santos,Izulmé Rita Imaculada.
The objective of this work was to adapt the application of electrolytic conductivity and potassium leaching tests to assess the viability of cryopreserved embryos of 'Anão Verde do Brasil de Jiqui' (AVeJBr) coconut. The zygotic embryos were excised, sterilized and subjected to four cryoprotectant treatments combined with three incubation times (12, 16 and 20 hours), totaling 12 treatments. The pre-treatment of mature zygotic embryos of AVeJBr coconut using a cryoprotectant with 1.75 mol L-1 of sucrose + 15% glycerol for 12 and 16 hours promoted lower embryo humidity and increased viability in electrolytic conductivity and potassium leaching tests. Samples with ten embryos are sufficient for electrolytic conductivity analysis in cryopreserved or...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocos nucifera; Biochemistry; Cell membrane; Cryoprotectant; Genetic resources.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000100002
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Freeze-drying of ampicillin solid lipid nanoparticles using mannitol as cryoprotectant BJPS
Alihosseini,Faezeh; Ghaffari,Solmaz; Dabirsiaghi,Ali Reza; Haghighat,Setareh.
abstract Solid lipid nanoparticles (SLNs) are interesting colloidal drug-delivery systems, since they have all the advantages of the lipid and polymeric nanoparticles. Freeze-drying is a widely used process for improving the stability of SLNs. Cryoprotectants have been used to decrease SLN aggregations during freeze-drying. In this study Ampicillin was chosen to be loaded in a cholesterol carrier with nano size range. To support the stability of SLNs, freeze-drying was done using mannitol. Particle size, drug release profile and antibacterial effects were studied after freeze-drying in comparison with primary SLNs. Preparations with 5% mannitol showed the least particle size enlargement. The average particle size was 150 and 187 nm before and after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid lipid nanoparticles; Stability; Cryoprotectant; Freeze-drying; Antibacterial efficacy; Particle size.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400797
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Induced reproduction of dourado (Salminus brasiliensis): fertilization with sperm cryopreserved in ACP®-104 Animal Sciences
Zanandrea, Ana Carolina Volpato; Weingartner, Marcos; Zaniboni-Filho, Evoy.
Experiments were conducted to improve the fertilization rate of cryopreserved semen of dourado, Salminus brasiliensis. The experiment tested two cryoprotectant solutions at different semen: cryoprotectant ratios (1:5, 1:15, 1:25 and 1:50). The standard solution for the species (mixture of dimethyl sulfoxide, glucose, egg yolk and distilled water) was compared to a 350 mOsm ACP®-104 solution, which is composed of powdered coconut water diluted in distilled water and methylglycol. Differences between the dilutions tested were significant only for ACP®. The fertilization potential by using the standard solution at the lowest dilution (1:5) is equated when the sperm is diluted in ACP® at 1:25 or 1:50. These results show that the standard solution is the most...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.06.03.04-3 cryopreservation; Sperm; Fertilization; Cryoprotectant; Coconut water.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/28838
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Long-term cryopreservation of basidiomycetes BJM
Linde,Giani Andrea; Luciani,Alana; Lopes,Ana Daniela; Valle,Juliana Silveira do; Colauto,Nelson Barros.
Abstract Basidiomycetes have several biotechnological and industrial applications such as enzyme production, bioremediation, pharmaceutical and functional food production. Due to climatic features, the preservation of several basidiomycetes is threatened, and to guarantee the preservation of this genetic resource, the development of long-term preservation techniques is necessary once there is no universal protocol for the cryopreservation of basidiomycetes. Cryopreservation is a technique in which microorganisms are submitted to ultralow temperatures. Therefore, this study aimed to collect information on the main conditions for long-term cryopreservation of basidiomycetes in the last 20 years. Scientific articles on cryopreservation of basidiomycetes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Preservation; Substrate; Fungi; Mycelial viability; Cryoprotectant.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200220
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Modified arrowroot starch and glucomannan for preserving physicochemical properties of sweet bread Ciência e Agrotecnologia
Damat,Damat; Setyobudi,Roy Hendroko; Soni,Peeyush; Tain,Anas; Handjani,Hany; Chasanah,Uswatun.
ABSTRACT Sweet bread is associated with smooth texture and high carbohydrate content, tasty and filling - quality that makes it preferred for snack. The key is in formulating raw material and other components. Commonly, sweet bread has short shelf life. Frozen dough could be the solution to this problem, and glucomannan addition during freezing process should be able to improve its physicochemical characteristics. This research aims to determine the effect of adding modified arrowroot (Maranta arundinacea L.) starch (MAS) as substitution, and glucomannan as frozen dough cryoprotectant toward sweet bread’s physicochemical. Randomized Complete Block Design Factorial (RCBD) was applied, and two factors were studied. The first factor was modified arrowroot...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryoprotectant; Frozen dough; Resistant fiber; Retrogradation; Shelf life.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542020000100405
Registros recuperados: 5
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