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Registros recuperados: 13
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Antigenic Diversity among Oocysts of Clinical Isolates of Cryptosporidium parvum OAK
Griffin, K.; Matthai, E.; Hommel, M.; Weitz, J. C.; Baxby, D.; Hart, C. A..
A panel of monoclonal antibodies has been produced against the protozoan parasite Cryptosporidium parvum which is a major cause of diarrhoeal disease in man and other animals. C. parvum oocysts from both a human and a bovine case of cryptosporidiosis were used as immunogens. A total of 11 different monoclonal antibodies were obtained which could bind to formalin-fixed oocysts. One was IgA but the remainder were all of IgM isotype. The reactivity of these monoclonal antibodies against a series of C. parvum oocysts obtained from 25 patients in Chile was examined using indirect immuno-fluorescence. Although a mixture of all of the 11 antibodies would have detected oocysts in each of the samples, no one monoclonal antibody recognised all oocysts. Each antibody...
Palavras-chave: Cryptosporidium parvum; Monoclonal antibody; Antigenic diversity.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/169
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Avaliação da eficácia do colostro bovino hiperimune na infecção experimental de roedores com Cryptosporidium parvum Braz. J. Vet. Res. Anim. Sci.
Abreu,Vicente José Salles de; Cardoso,Ary Lopes; Pena,Hilda Fátima de Jesus; Gennari,Solange Maria; Sinhorini,Idércio; Damy,Sueli Blanes.
O colostro bovino hiperimune contém alta concentração de anticorpos anti- Cryptosporidium parvum. Este produto é considerado como uma das estratégias terapêuticas promissoras no controle da criptosporidiose intestinal em humanos. Com o objetivo de avaliar a eficácia do colostro bovino hiperimune na redução do parasitismo e na prevenção de alterações de mucosa intestinal, foram infectados, experimentalmente, com oócistos de Cryptosporidium parvum, vários ratos (F344) e camundongos (C57BL/6). Os resultados mostraram que o colostro bovino hiperimune apresentou altos títulos de anticorpos. Os ratos F344, tratados com colostro bovino hiperimune ou com colostro normal, tiveram redução no parasitismo intestinal e apresentaram menor comprometimento de sua mucosa....
Tipo: Info:eu-repo/semantics/other Palavras-chave: Colostro; Modelos animais de doenças; Cryptosporidium parvum; Roedores; Ratos endogâmicos F344.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000900006
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Bench Scale Experiments to Envaluate the Usefulness of Sucrose Flotation Techniques for Separation of Cryptosporidium Oocysts from Water OAK
Kimura, A.; Karanis, Panagiotis; Maltezos, E.; Seitz, H. M..
Palavras-chave: Cryptosporidium parvum; Modified sucrose flotation technique; Discontinuous sucrose gradients; Separation of oocysts; Water borne disease.
Ano: 2000 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/342
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Construction of Polymerase Chain Reaction Primer to Detect Cryptosporidium parvum or C. muris OAK
Zhiliang, Wu; Iseki, Motohiro; Kimata, Isao; Dong, Xu; Nagano, Isao; Takahashi, Yuzo.
Palavras-chave: Cryptosporidium parvum; Cryptosporidium muris; PCR; Primer.
Ano: 1995 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/236
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Construction of the recombinant pseudorabies viruses expressing Cryptosporidium parvum an immunodominant surface protein, p23 OAK
Takashima, Yasuhiro; Xuan, Xuenan; Nagasawa, Hideyuki; Matsumoto, Yasunobu; Igarashi, Ikuo; Fujisaki, Kozo; Mikami, Takeshi; Otsuka, Haruki; 玄, 学南; 五十嵐, 郁男.
To develop a vaccine against cryptosporidiosis in animals, we constructed recombinant pseudorabies virus (PrV), a member of the Herpesviridae Alphaherpesvirus subfamily, expressing an immunodominant surface protein p23 of Cryptosporidium parvum sporozoites. Because of the wide host range of PrV, it has the possibility as the vector to delivery the foreign genes to several species of animals containing experiment animal. In the recombinant constructed in this study, the p23 gene under the control of CAG promoter was integrated into the thymidine kinase (TK) gene of PRV. Antibody against p23 recognized p23 expressed in CPK cells infected with the recombinant, as the approximate 23 kDa specific band in Western blotting analysis. This study showed the...
Palavras-chave: Cryptosporidium parvum; P23; Herpes; Pseudorabies virus; Subunit vaccine.
Ano: 2000 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/129
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Cryptosporidial Infection of Pigs at The Intensive Breeding OAK
Pavlović, Ivan; Ivetic, Vojin; Erski-Biljic, Milanka; Milutinovic, Marija; Kulišić, Zoran.
Palavras-chave: Cryptosporidium parvum; Pigs; Neonatal diarrhea; Cryptosporidiosis.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/241
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Development and evaluation of an enzyme-linked immunosorbent assay using recombinant p23 for the detection of antibodies to Cryptosporidium parvum in cattle OAK
Bannai, Hiroshi; Nishikawa, Yoshifumi; Seo, Jin-yong; Nakamura, Chinatsu; Zhang, Sofa; Kimata, Isao; Takashima, Yasuhiro; Li, Junyou; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南.
An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%)....
Palavras-chave: Cryptosporidium parvum; P23; ELISA; IFAT.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/142
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Diagnostic Methods for the Detection of Cryptosporidium parvum Oocysts in Faeces OAK
Webster, K. A.; Green, J. A.; Dawson, C.; Giles, M.; Catchpole, J..
Palavras-chave: Cryptosporidium parvum; Diagnosis; Immunofluorescence; ELISA; Coproscopical.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/253
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Excreção de oocistos de Cryptosporidium parvum em cães saudáveis das cidades de Lavras e Viçosa, Estado de Minas Gerais, Brasil Ciência Rural
Figueiredo,Henrique César Pereira; Pereira Júnior,Delton José; Nogueira,Rodrigo Bernardes; Costa,Paulo Renato dos Santos.
Com o objetivo de determinar a freqüência de excreção de Cryptosporidium parvum foram coletadas 269 amostras de fezes de cães saudáveis, de áreas urbanas e rurais, dos municípios de Lavras e Viçosa, Estado de Minas Gerais. A detecção de oocistos foi realizada por um kit de ELISA direto (Alexon - Trend, USA). Do total de amostras analisadas, cinco (1.85%) foram positivas para a excreção de C. parvum. Não houve diferença entre a freqüência de excreção para cães da área urbana e rural.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryptosporidium parvum; Cães; Oocistos.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000500049
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Genotype and subtype analyses of Cryptosporidium isolates from cattle in Hungary OAK
Plutzer, Judit; Karanis, Panagiotis.
Seventy-nine faecal samples from calves with diarrhoea were collected on 52 farms from different counties in Hungary to investigate the species, genotypes and subtypes of Cryptosporidium in calves. Oocysts from faecal sample collected from each animal were concentrated using sucrose gradient centrifugation and examined by immunofluorescence assay (IFA). Genomic DNA was extracted from microscopically positive samples and nested PCR was performed to amplify the partial SSU rRNA and GP60 genes of Cryptosporidium that were subsequently digested by SspI, VspI and MboII restriction enzymes to determine the Cryptosporidium species and genotype present. Cryptosporidium parvum was detected in 21 samples while the Cryptosporidium deer-like genotype was found in...
Palavras-chave: Cryptosporidium parvum; Cattle; Genotype; Subgenotype; Hungary.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1056
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Prevalência de criptosporidiose em bezerros na região de Araçatuba, Estado de São Paulo, Brasil Ciência Rural
Feitosa,Francisco Leydson Formiga; Shimamura,Graziele Massae; Roberto,Thaís; Meireles,Marcelo Vasconcelos; Nunes,Caris Maroni; Ciarlini,Paulo César; Borges,Alexandre Secorum.
Avaliou-se a prevalência de oocistos de Cryptosporidium parvum em amostras de fezes de 459 bezerros com até 30 dias de idade e em amostras de água e piso dos bezerreiros de 33 propriedades leiteiras na região de Araçatuba-Estado de São Paulo. A maior porcentagem de excreção de oocistos foi verificada em bezerros com faixa etária variando entre oito e 14 dias de idade (14,5%) sendo, a menor taxa (6,4%), detectada no grupo de animais mais velhos (22 a 30 dias de vida). Observaram-se, na amostragem total, valores positivos aproximados de 10,26% pelo ensaio de imunoadsorção enzimática e de 12,4% pelo teste de Sheather. As amostras de água foram negativas e duas de solo positivas.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Criptosporidiose; Cryptosporidium parvum; Bezerros.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000100029
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Serological detection and epidemiology of Neospora caninum and Cryptosporidium parvum antibodies in cattle in southern Egypt OAK
Fereig, Ragab M.; AbouLaila, Mahmoud Rezk; Mohamed, Samy G. A.; Mahmoud, Hassan Y. A. H.; Ali, Alsagher O.; Ali, Asmaa F.; Hilali, Mosaad; Zaid, Anis; Mohamed, Adel Elsayed Ahmed; Nishikawa, Yoshifumi.
Neospora caninum and Cryptosporidium parvum are intracellular protozoan parasites that are distributed worldwide and of major economical concern in cattle industry. N. caninum can cause abortion storms and high culling rates, whereas C. parvum has zoonotic implications and can cause diarrhea in calves. There are currently no data on the prevalence of neosporosis and cryptosporidiosis in humans or animals in southern Egypt. Prevalence of these two infections was determined in a sample of cattle from two different areas in southern Egypt, Sohag and Qena, using enzyme-linked immunosorbent assay. A total 301 cattle were sampled, of which 18.9% were positive for N. caninum, 35.9% were positive for C parvum and 10.0% were positive for both. Geographical location...
Palavras-chave: Cattle; Cryptosporidium parvum; Neospora caninum; ELISA; Egypt.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4531
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The Low Sedimentation Speed of Cryptosporidium Oocysts : A Further Explanation for Waterborne Outbreaks OAK
Sréter, T.; Széll, Z..
Palavras-chave: Cryptosporidium parvum; C. muris; C. baileyi; Cryptosporidiosis; Waterborne transmission; Sedimentation.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/278
Registros recuperados: 13
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