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| Registros recuperados: 36 | |
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| Suarez,Carlos Alberto Galeano; Montano,Inti Doraci Cavalcanti; Nucci,Edson Romano; Iemma,Monica Rosas da Costa; Giordano,Raquel de Lima Camargo; Giordano,Roberto de Campos. |
| Bacillus megaterium is a promising host for expression of heterologous proteins. This paper reports the nutrient consumption patterns and production of metabolites for three different strains of B. megaterium, ATCC 14945, QMB 1551 and PV 361, which is QMB 1551 with seven constitutive plasmids deleted. 14 h cultivations in agitated flasks were run, for two different media: A (LB plus 10g/L glucose) and B (medium A, with the yeast extract replaced by tryptone). Strains PV361 and QMB 1551 showed higher maximum specific growth rates in medium B, reaching 0.42 h-1 and 0.48 h-1 respectively. The main by-products of the glucose overflow mechanism were acetate and lactate, for all three strains, which had preferential amino acids for substrate: Ala, Asp, Glu, Ser.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metabolic profile; Bacillus megaterium; Culture media. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400001 |
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| PEREIRA,GUILLERMO; ALBORNOZ,VERÓNICA; ROMERO,CHRISTIAN; LARA,SEBASTIÁN; SÁNCHEZ-OLATE,MANUEL; RÍOS,DARCY; ATALA,CRISTIAN. |
| ABSTRACT Orchids require symbiotic fungi and/or specific conditions to germinate. Asymbiotic techniques have been shown successful for orchid germination. In Chile, Chloraea include many endemic, and potentially ornamental, terrestrial orchid species. In this study, individuals of Chloraea crispa, C. gavilu and C. virescens were manually autopollinated. The resulting capsules were sterilized and seeds were aseptically obtained. We evaluated asymbiotic germination in: Agar Water (AW), Knudson C (KC), Banana Culture Media (CMB), Tomato Culture Media (CMT), Malmgren Modified (MM), Murashige and Skoog (MS), and MS modified (MS1/2). Seeds were incubated in the dark at 24±1 ºC for 2 weeks. Then they were put in 16/8 h light/dark cycles for 14 weeks. We... |
| Tipo: Journal article |
Palavras-chave: Terrestrial orchids; Endemic species; In vitro germination; Culture media; Orchid propagation; Asymbiotic germination. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432017000100131 |
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| Morais-Lino,Lucymeire Souza; Santos-Serejo,Janay Almeida dos; Silva,Sebastião de Oliveira e; Santana,José Raniere Ferreira de; Kobayashi,Adilson Kenji. |
| The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Musa; Culture media; Propagation; Somatic embryogenesis. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000010 |
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| Alvarado Castillo, Gerardo. |
| Morchella esculenta es un hongo comestible de alto valor comercial y la obtención de esclerocios es considerada como la clave para su cultivo. En este trabajo se evaluó la cepa IE-750 en ocho medios de cultivo sólido y seis en forma líquida, utilizando como parámetros el crecimiento micelial, la producción de biomasa, la habilidad para producir esclerocios y su biomasa. El mejor crecimiento micelial se obtuvo en el tratamiento con composta (T7: 53.87 cm2), y en cuanto a la biomasa, el medio de cultivo con levadura fue mejor (T3: 80.3 mg). Los tratamientos con gallinaza y composta, presentaron 40% y 80% de esclerocios, respectivamente, tanto en medio sólido como líquido. La mayor cantidad de biomasa de estas estructuras se presentó en el... |
| Tipo: Tesis |
Palavras-chave: Esclerocios; Composta; Medios de cultivo; Morchella esculenta; Maestría; Agroecosistemas Tropicales; Sclerotia; Compost; Culture media. |
| Ano: 2008 |
URL: http://hdl.handle.net/10521/1414 |
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| Tresierra-Ayala,Álvaro; Navas,Manuel; Flores,Josué; Perea,Ramsés; Huanaquiri,Juan; Bendayán,María; Fernández,Heriberto. |
| In this work 60 thermotolerant Campylobacter strains (37 C. jejuni and 23 C. coli) isolated from the cows, pigs, chickens and ducks (15 strains of each type of animal) were used to establish their growth capacity on media containing cow or swine blood as potential substitutes of sheep or horse blood. The growth capacity was assessed by viable counts on cow and swine blood media, using the modified Miles and Misra method. Campylobacter strains showed better growth in the media supplemented with pig or sheep blood than with cow blood. Thus, the use of pig blood could be a supplement for Campylobacter culture medium, when there was no availability of sheep or horse blood. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter; Culture media; Cow blood; Swine blood. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500011 |
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| Registros recuperados: 36 | |
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