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Registros recuperados: 19
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Antileishmanial activity of extracts from Libidibia ferrea: development of in vitro and in vivo tests 36
COMANDOLLI-WYREPKOWSKI,Claudia Dantas; JENSEN,Bruno Bezerra; GRAFOVA,Iryna; SANTOS,Pierre Alexandre dos; BARROS,Angela Maria Comapa; SOARES,Fabiane Veloso; BARCELLOS,José Fernando Marques; SILVA,Aline Fagundes da; GRAFOV,Andriy; FRANCO,Antonia Maria Ramos.
ABSTRACT Treatment of cutaneous leishmaniasis (CL) is difficult due to the scarce number of drugs able to eliminate completely the intracellular form of the parasite. In the present study, the aim was to evaluate: i) phytochemical properties of extracts from Libidibia ferrea; ii) antileishmanial activity of extracts from L. ferrea against promastigotes and amastigotes of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) guyanensis; iii) the effects of topical treatment using hydrogel containing active extract of L. ferrea on golden hamsters infected with L. (L.) amazonensis. Extracts from leaves, branches and fruits of L. ferrea were obtained with hexane and methanol and were tested by in vitro assays in promastigotes and murine macrophages J774...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutaneous leishmaniasis; Natural products; Topical formulations; Experimental treatment.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000400331
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Assessment of Leishmania major and Leishmania braziliensis promastigote viability after photodynamic treatment with aluminum phthalocyanine tetrasulfonate (AlPcS4) 80
JG,Pinto; CP,Soares; J,Mittmann.
Cutaneous leishmaniasis is an infectious disease caused by protozoans of the genus Leishmania, which is transmitted through the bite of hematophagous insects of the genus Lutzomyia. This study aimed at testing in vitro the phototoxic effect of aluminum phthalocyanine tetrasulfonate (AlPcS4) on the viability of Leishmania major and Leishmania braziliensis. Stationary phase promastigote forms were treated with AlPcS4 at 1.0 µM and 10.0 µM and incubated for one hour. Then 659 nm laser was applied at 5 and 10 J/cm². Parasite viability was determined by differential count using the trypan blue dye exclusion method and by monitoring growth curves for nine days. Trypan blue exclusion assay showed a significant reduction of viable parasites compared to controls,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutaneous leishmaniasis; Antiparasitic agents; Leishmania major; Leishmania braziliensis; AlPcS4; Photodynamic therapy.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300010
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Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil 55
Szargiki,Regiane; Castro,Edilene Alcântara de; Luz,Ennio; Kowalthuk,Wolodymir; Machado,Ângela Maria; Thomaz-Soccol,Vanete.
We evaluated the effectiveness of serological and parasitological methods for cutaneous leishmaniasis (CL) diagnosis in patients from the central region of Paraná state, southern Brazil. Five groups were compared: clinical diagnosis, parasitological diagnosis, communicants, inhabitants of a non-endemic area and carriers of other etiologies. Two antigens were prepared from promastigotes of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis for indirect immunofluorescence assay, ELISA and immunoblotting. The parasitological approaches detected 79.3% of the patients with a clinical diagnosis; the parasites were identified by PCR as L. (V.) braziliensis. Serological methods showed 95% sensitivity for homologous antigens. Immunoblotting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutaneous leishmaniasis; Immunodiagnosis; Antigen; Western blotting.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100011
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Comparison of the specificity of PCR and the histopathological detection of leishmania for the diagnosis of American cutaneous leishmaniasis 56
Medeiros,A.C.R.; Rodrigues,S.S.; Roselino,A.M.F..
More precise and rapid diagnostic methods for American cutaneous leishmaniasis (ACL) are necessary because of the growing number of cases observed in Brazil, including the northeastern region of the State of São Paulo. We applied PCR to 54 skin or mucosal biopsies from patients with a clinical and/or laboratory diagnosis of ACL using primers 13A and 13B, with positive results being obtained for 82% of the samples. When the PCR results were compared to those of histopathological leishmania detection, PCR showed superior results with 81.5% sensitivity and 95% CI of 68.0-95.1%. The Montenegro skin test (MST) was positive in 88.7% of patients. Since MST cannot be used as a diagnostic tool in endemic areas, the present results strongly suggest the use of PCR...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Cutaneous leishmaniasis; PCR; Histopathological detection; Leishmania detection.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400002
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Diagnosis of American cutaneous leishmaniasis by enzyme immunoassay in patients from Northern Paraná State, Brazil 55
Yoneyama,Kelly Aparecida Geraldo; Peder,Leyde Daiane de; Lonardoni,Maria Valdrinez Campana; Silveira,Thaís Gomes Verzignassi.
American cutaneous leishmaniasis (ACL) is an endemic disease caused by Leishmania parasites. The ACL diagnosis is commonly accomplished by parasitological and immunological methods. The objective of this work was to evaluate the enzyme immunoassay (EIA) for ACL laboratorial diagnosis. IgG antibodies against Leishmania (Viannia) braziliensis promastigotes were researched. For the method standardization 240 sera were used: 72 from patients with positive parasitological diagnosis, 38 from normal individuals and 113 from individuals with other pathologies. The sensibility was 93% and the specificity was 70%. Regarding diagnosis, EIA showed 97% positivity in patients with ACL and 97% negativity in patients with other cutaneous lesions. The EIA presented a good...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutaneous leishmaniasis; Diagnosis; Antibodies; Immunoenzyme; Leishmania braziliensis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300012
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Domestic feline cutaneous leishmaniasis in the municipality of Ribas do Rio Pardo, Mato Grosso do Sul state, Brazil: a case report 80
Souza,AI; Nunes,VLB; Borralho,VM; Ishikawa,EAY.
Cutaneous leishmaniases are anthropozoonoses that involve many species of Leishmania and a wide variety of wild mammalian hosts, thus presenting high importance to public health. This study reports the second case of feline leishmaniasis in Mato Grosso do Sul state, in which Leishmania (Leishmania) amazonensis was found in a domestic cat from Ribas do Rio Pardo. Clinical signs were similar to those observed in other diseases commonly diagnosed in cats, such as cryptococcosis and sporotrichosis. Cutaneous leishmaniasis should, therefore, be added to differential diagnoses by feline veterinary practitioners, and also adequate investigations should be carried out to verify the relevance of domestic cats as L. amazonensis reservoirs.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutaneous leishmaniasis; Leishmania (Leishmania) amazonensis; Domestic cat; Mato Grosso do Sul state.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200017
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Electrocardiographic changes during low-dose, short-term therapy of cutaneous leishmaniasis with the pentavalent antimonial meglumine 56
Ribeiro,A.L.P.; Drummond,J.B.; Volpini,A.C.; Andrade,A.C.; Passos,V.M.A..
The pentavalent antimonial (Sb5+) meglumine is the drug of choice for the treatment of cutaneous leishmaniasis (CL) in Brazil. Although the cardiotoxicity of high-dose, long-term Sb5+ therapy is well known, the use of low-dose, short-term meglumine has been considered to be safe and relatively free from significant cardiac effects. In order to investigate the cardiotoxicity of low-dose, short-term therapy with meglumine in cutaneous leishmaniasis, 62 CL patients treated with meglumine were studied. A standard ECG was obtained before and immediately after the first cycle of treatment (15 mg Sb5+ kg-1 day-1). The electrocardiographic interpretation was carried out blindly by two investigators using the Minnesota Code. There were no significant differences in...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cutaneous leishmaniasis; Electrocardiography; QT interval.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300008
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Epidemiological and clinical characteristics of cutaneous leishmaniasis and their relationship with the laboratory data, south of Brazil 55
Curti,Maura Cristiani de Moura; Silveira,Thaís Gomes Verzignassi; Arraes,Sandra Mara Alessi Aristides; Bertolini,Dennis Armando; Zanzarini,Paulo Donizeti; Venazzi,Eneide Aparecida Sabaini; Fernandes,Andrea Cláudia Silva; Teixeira,Jorge Juarez Vieira; Lonardoni,Maria Valdrinez Campana.
OBJECTIVE: To evaluate clinical, epidemiological and laboratorial aspects for the understanding of the disease characteristics and its relationship with diagnostic tests. METHODS: A retrospective, descriptive and analytical study involving 2,660 American cutaneous leishmaniasis (ACL) suspected patients from southern Brazil was undertaken between April 1986 and December 2005. Data on population characteristics and laboratory tests were obtained. Diagnostic laboratory tests used were direct search for Leishmania spp. (DS), Montenegro skin test (MST) and indirect immunofluorescent assay (IFA). RESULTS: 62.3% of patients were positive for at least one laboratory test. DS test was positive in 65.1%; MST in 92.3% and IFA in 70.0%. Although Cohen's Kappa test did...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clinical laboratory techniques; Disease transmission; Infectious; Cutaneous leishmaniasis; Epidemiology.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100003
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Expansion of gd T cells in patients infected with cutaneous leishmaniasis with and without glucantime therapy 55
Darabi,Haideh; Abolhassani,Mohsen; Kariminia,Amina; Alimohammadian,Mohammad H..
The expansion of <FONT FACE=Symbol>gd </font>T cells in patients with active cutaneous leishmaniasis, with or without glucantime therapy, was investigated. Twenty patients with local cutaneous leishmaniasis including glucantime-treated (n=10) and untreated (n=10) patients were selected. The controls were healthy individuals (n=10) living in endemic areas. Whole blood was obtained and the T cell subpopulations were analyzed by flow cytometry. Significantly more <FONT FACE=Symbol>gd</FONT> CD3+ T cells were observed in untreated patients (15.9% ± 5.9), when compared with glucantime-treated patients (4.6% ± 1.4) and controls (5.3% ± 2.3). On the other hand, when the percentages of ab CD3+ T-cells were analyzed different results were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutaneous leishmaniasis; Gamma-delta T cells; Glucatime therapy.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000500007
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Impact of leishmaniasis on public health 80
Camargo,L. B; Langoni,H..
Leishmaniasis is a parasitic zoonosis caused by protozoans of the genus Leishmania transmitted by insects known as phlebotomines, which are found in wild or urban environments. It affects domestic and wild animals and transmission to man happens by accident. The disease occurs in tropical and sub-tropical areas, mainly in Asia, Europe, Africa, and the Americas. There are two forms that affect man: American cutaneous leishmaniasis (ACL) and American visceral leishmaniasis (AVL). The latter is caused by three species of Leishmania: Leishmania (Leishmania) donovani, Leishmania (Leishmania) infantum, and Leishmania (Leishmania) chagasi, which are grouped in the Leishmania (Leishmania) donovani complex. Wild reservoir hosts of L. chagasi known so far are foxes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Cutaneous leishmaniasis; Visceral leishmaniasis; Leishmania.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400002
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Investigation of natural infection by Leishmania in sandflies of Paraná State, Southern Brazil 52
Scodro,Regiane Bertin de Lima; Reinhold-Castro,Kárin Rosi; Dias-Sversutti,Alessandra de Cassia; Neitzke-Abreu,Herintha Coeto; Membrive,Norberto de Assis; Kühl,João Balduíno; Silveira,Thaís Gomes Verzignassi; Teodoro,Ueslei.
The purpose of this work was to verify the occurrence of Leishmania in naturally infected sandflies. The insects were collected with Falcão, Shannon and HP light-traps, in Doutor Camargo and Maringá municipalities between November 2004 and October 2005. Of the 11,033 sandflies collected in Doutor Camargo, 2,133 surviving females were dissected, particularly those of the Nyssomyia neivai species (86.87%). In Maringá, 136 sandflies were collected, of which 79 N. whitmani females and 1 Migonemyia migonei female were dissected. The dissected insects were identified and stored in the pools of 10 specimens. The PCR was carried out on 1,190 females of N. neivai and 190 of N. whitmani from Doutor Camargo, and on 30 of N. whitmani from Maringá, using the primers...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutaneous leishmaniasis; Sandflies; Dissection; Polymerase chain reaction.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300007
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Nyssomyia intermedia (Lutz &amp; Neiva) and Nyssomyia neivai (Pinto) (Diptera, Psychodidae, Phlebotominae) in a sympatric area: seasonal and nocturnal hourly rhythm in black and white modified Shannon traps 92
Galati,Eunice A. B.; Marassá,Ana Maria; Gonçalves-Andrade,Rute Maria; Bueno,Edna Fátima Maria; Paiva,Byanca R.; Malafronte,Rosely S..
Captures with black and white Shannon traps were undertaken to identify aspects of the behavior of the two cryptic and sympatric species implicated as vectors of cutaneous leishmaniasis, Nyssomyia intermedia (Lutz &amp; Neiva, 1912) and Nyssomyia neivai (Pinto, 1926). The traps were installed side by side, monthly, from July 2001 to June 2002, from 18 to 07 hours, in a peridomicile of Iporanga municipality, state of São Paulo, Brazil. A total of 2,142 specimens were captured, Ny. intermedia (47.4%) and Ny. neivai (50.5%). The white trap was more attractive to both sexes of both species. Males of Ny. neivai predominated (70%) over those of Ny. intermedia on the two traps; on the black trap, the females of Ny. intermedia predominated (63.3%) over those...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ecology; Cutaneous leishmaniasis; Ribeira Valley; Vectors.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262010000400021
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Pharmacokinetics of neutron-irradiated meglumine antimoniate in Leishmania amazonensis-infected BALB/c mice 80
Borborema,Samanta Etel Treiger; Osso Junior,João Alberto; Andrade Junior,Heitor Franco de; Nascimento,Nanci do.
Abstract Background: Cutaneous leishmaniasis (CL) is a parasitic disease caused by the protozoan Leishmania spp. Pentavalent antimonial agents have been used as an effective therapy, despite their side effects and resistant cases. Their pharmacokinetics remain largely unexplored. This study aimed to investigate the pharmacokinetic profile of meglumine antimoniate in a murine model of cutaneous leishmaniasis using a radiotracer approach. Methods: Meglumine antimoniate was neutron-irradiated inside a nuclear reactor and was administered once intraperitoneally to uninfected and L. amazonensis-infected BALB/c mice. Different organs and tissues were collected and the total antimony was measured. Results: Higher antimony levels were found in infected than...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutaneous leishmaniasis; Meglumine antimoniate; Pharmacokinetics; Biodistribution; Antimony; Radioisotope.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100304
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PREVALENCE OF CUTANEOUS LEISHMANIASIS IN PARTS OF KADUNA STATE, NIGERIA 19
Okwori, E. N. A.; Nock, I. H.; Galadima, M.; Ibrahim, S..
A cross-sectional survey of 29 randomly selected schools, comprising 11 (37.9%) post-primary schools and 18 (62.1%) primary schools from eight Local Government Areas (LGAs) (approximately 35% of the 23 LGAs) of Kaduna State was undertaken to determine the prevalence status of cutaneous leishmaniasis between May and October, 1997. The disease occurrence was detected by on-sight diagnosis of active lesions and scars, and observed leishmanial cases were recorded for patients who gave description of symptoms that fitted into clinical symptoms of cutaneous leishmaniasis. The recorded cases were confirmed by parasitological examination of Giemsa-stained lesion smears for amastigotes in the laboratory. The Chi-square analysis of the result revealed that there was...
Palavras-chave: Cutaneous leishmaniasis; Leishmania tropica; L. major; Phlebotomus (Phlebotomus) duboscqui; Amastigote.
Ano: 2001 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/136
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Reorganization and cleanness of peridomiciliar area to control sand flies (Diptera, Psychodidae, Phlebotominae) in South Brazil 52
Teodoro,Ueslei; Thomaz-Soccol,Vanete; Kühl,João B.; Santos,Demilson Rodrigues dos; Santos,Elcio Silvestre dos; Santos,Ademar Rodrigues dos; Abbas,Milia; Dias,Alessandra de Cassia.
The objective of the present work was to evaluate the impact of the reorganization and cleaning of the peridomicily seeking the control of Phlebotominae vectors of Leishmania. Collections of insects were made in domestic animal shelters and in peridomiciliar area located in Jussara farm, Municipality of Jussara, State of Parana, Brazil where cutaneous leishmaniasis has been endemic. The collections were accomplished five nights for month of the 21:00pm for fie hours, in 1996 and 1997. The results were compared the those obtained in the same places in 1992 and 1994. After the collections of 1992 peridomiciliar area was cleaned and organic matter was removed and domestic animals were reallocated. In 1992, 1994 and 1996/97, 10, 7 and 12 Phlebotominae species...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phlebotominae control; Leishmaniasis control; Lutzomyia whitmani; Cutaneous leishmaniasis.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200007
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Sand Flies (Diptera: Psychodidae) in the urban area of the municipality of Cianorte, Paraná State, Brazil 82
Cerino,Daniela A; Teodoro,Ueslei; Silveira,Thaís G V.
The endemicity of American cutaneous leishmaniasis (ACL) in the Cianorte municipality, Paraná State the occurrence of human cases with the probable infection locus in the urban area, the presence of a permanently preserved modified native forest in the urban perimeter, and the lack of knowledge of the fauna of sand flies in the municipality provided the impetus for this study. The objective of this study was to assess the fauna, frequency and seasonality of the sand flies in the peridomicile, forest and urban area of this municipality. Sand flies were collected using Falcão light traps installed in the peridomicile and forest, from July 2005 to June 2006. A total of 755 sand flies were collected; Nyssomyia whitmani (Antunes &amp; Coutinho) (84.0%),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutaneous leishmaniasis; Urban area; Native forest.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2009000600021
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Sandflies (Diptera, Psychodidae) from forest areas in Botucatu municipality, central western São Paulo State, Brazil 80
Cutolo,André Antonio; Galati,Eunice Aparecida Bianchi; Von Zuben,Claudio José.
Background The study of the distribution and ecology of sandfly species is essential for epidemiological surveillance and estimation of the transmission risk of Leishmania spp. infection. Findings In the present study, sandflies were captured in native fragmented forest areas in Rubião Júnior district, Botucatu municipality, São Paulo state, Brazil, between September 2001 and January 2005. A minimum of two automatic light traps were installed per night from 6 pm to 8 am, in different months, resulting in approximately 900 collecting hours. During this period, 216 sandfly specimens of sixteen species were captured. Pintomyia monticola and Brumptomyia guimaraesi were the most abundant with 56 specimens (25.93%) captured per species, followed by Pintomyia...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cutaneous leishmaniasis; Vector; Sandfly; Brazilian savannah; Semideciduous forest; Cuesta.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100403
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The role of complement in the early phase of Leishmania (Leishmania) amazonensis infection in BALB/c mice 56
Laurenti,M.D.; Örn,A.; Sinhorini,I.L.; Corbett,C.E.P..
Complement-depleted and -non-depleted BALB/c mice were inoculated with Leishmania (Leishmania) amazonensis promastigotes into the hind footpad to study the role of the complement system in cutaneous leishmaniasis. Total serum complement activity was measured by hemolytic assay and C3 fragment deposit at the inoculation site was determined by direct immunofluorescence in the early period of infection, i.e., at 3, 24, 48 h and 7 days post-infection. The inflammatory reaction and the parasite burden were evaluated in the skin lesion at 7 and 30 days post-infection. Total serum complement activity decreased in the early phase of infection, from 3 to 24 h, in non-depleted mice compared to non-infected and non-depleted mice. C3 fragment deposit at the site of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutaneous leishmaniasis; Leishmania (Leishmania) amazonensis; Complement; Pathology.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300021
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The role of natural killer cells in the early period of infection in murine cutaneous leishmaniasis 56
Laurenti,M.D.; Gidlund,M.; Ura,D.M.; Sinhorini,I.L.; Corbett,C.E.P.; Goto,H..
In order to study the role of natural killer (NK) cells during the early period of Leishmania infection, BALB/c mice were selectively and permanently depleted of NK cells by injection with 90Sr and subsequently infected with Leishmania (Leishmania) amazonensis (HSJD-1 strain). 90Sr is known to selectively deplete NK cells, leaving an intact T- and B-cell compartment and preserving the ability to produce both interferon alpha and IL-2. This method of depletion has advantages when compared with depletion using anti-NK cell monoclonal antibodies because the effect is permanent and neither activates complement nor provokes massive cell death. In the present study, after one month of treatment with 90Sr, the depletion of NK cells was shown by a more than...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cutaneous leishmaniasis; NK cells; Strontium 90; Leishmania (Leishmania) amazonensis.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300012
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