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	Provedor de dados Rev. Bras. Zool. (1) Electron. J. Biotechnol. (1) Autor Chen,Jianhua (1) Gao,Huan (1) Jia,Rui (1) Nie,Liu-Wang (1) Wang,Cuihua (1) Wang,Jingjing (1) Wang,Ning (1) Yan,Binlun (1) Mais... Palavra-chave DEAD-box (2) Aquatic environments (1) BPA (1) Endocrine disrupting (1) Expression analysis (1) Expression patterns (1) Histological observation (1) Phylogenetic analysis (1) RT-PCR (1) Vasa (1) Vasa cDNA (1) Vasa gene (1) Vasa mRNA (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2016 (1) 2009 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Expression analysis of vasa in Asian paddle crab (Charybdis japónica) exposed to Bisphenol A Electron. J. Biotechnol. Chen,Jianhua; Wang,Cuihua; Gao,Huan; Yan,Binlun. Background: Bisphenol A (BPA) is an endocrine-disrupting chemical (EDC) with a weak estrogen-like activity in fish that is found ubiquitously in aquatic environments. However, there has been little study about BPA on the endocrine disrupting effects of crab. In the present study, cDNA of vasa was cloned and characterized in the Charybdis japonica. Histological structures of testis and expression patterns of vasa gene in the testis of C. japonica after treatment with BPA were investigated. Results: The cDNA of vasa is composed of 3051 bp with a 2166 bp open reading frame encoding 721 AA. The deduced amino acid sequence contained eight conserved domains of the DEAD-box protein family. The tissue distribution showed that vasa mRNA was specifically expressed... Tipo: Journal article Palavras-chave: Aquatic environments; BPA; DEAD-box; Endocrine disrupting; Expression analysis; Expression patterns; Histological observation; Vasa; Vasa cDNA; Vasa gene; Vasa mRNA. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600008 Molecular cloning and expression patterns of the Vasa gene from Rana nigromaculata (Amphibia: Anura) Rev. Bras. Zool. Jia,Rui; Nie,Liu-Wang; Wang,Ning; Wang,Jingjing. The Vasa protein is a member of the DEAD (Asp-Glu-Alu-Asp) box family of ATP-dependent RNA helicases. The Vasa gene is specifically expressed in germ-line cells of many metazoans and is known to play a critical role in gametogenesis and reproductive regulation. In this paper, we isolate the full length cDNA sequence of the Vasa gene from the frog Rana nigromaculata Hallowell, 1861. The open reading frame (ORF) encoding 398 amino acid residues has nine conserved motifs. According to the similarities at the amino acid sequenceythe phylogenetic analysis of Vasa gene was consistent with the evolution relationships from chordates to mammals. Furthermore, the expression pattern analysis of RnVasa mRNA, using the technique of Reverse Transcriptase-Polymerase... Tipo: Info:eu-repo/semantics/article Palavras-chave: DEAD-box; Phylogenetic analysis; RT-PCR. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702009000200014 Registros recuperados: 2 Primeira ... 1 ... Última

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