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| Barranger, Audrey; Langan, Laura M; Sharma, Vikram; Rance, Graham A; Aminot, Yann; Weston, Nicola J; Akcha, Farida; Moore, Michael N; Arlt, Volker M; Kholbystov, Andrei N; Readman, James W; Jha, Awadhesh N.. |
| This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by GC-MS, LC-HRMS and ICP-MS. Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the toxic effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1 mg/L of C60, the majority of which were down-regulated (~52%). No DEPs were identified at any of the concentrations of nC60 (p < 0.05, 1% FDR).... |
| Tipo: Text |
Palavras-chave: Trojan Horse effect; B[a]P; NC60; Co-exposure; Mussels; DNA damage; Proteomics. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00498/60946/64340.pdf |
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| Barranger, Audrey; Langan, Laura M.; Sharma, Vikram; Rance, Graham A.; Aminot, Yann; Weston, Nicola J.; Akcha, Farida; Moore, Michael N.; Arlt, Volker M.; Khlobystov, Andrei N.; Readman, James W.; Jha, Awadhesh N.. |
| This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by Gas Chromatography–Mass Spectrometry (GC–MS), Liquid Chromatography–High Resolution Mass Spectrometry (LC–HRMS) and Inductively Coupled Plasma Mass Spectrometer (ICP–MS). Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the interactive effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1... |
| Tipo: Text |
Palavras-chave: Trojan horse effect; B[a]P; NC(60); Co-exposure; Mussels; DNA damage; Proteomics. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00506/61780/65779.pdf |
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| Singh,Thangjam Davis; Meitei,Heikrujam Thoihen; Sharma,Adhikarimayum Lakhikumar; Robinson,Asem; Singh,Lisam Shanjukumar; Singh,Thiyam Ramsing. |
| BACKGROUND: Clinical use of chemotherapeutic drug, cisplatin is limited by its toxicity and drug resistance. Therefore, efforts continue for the discovery of novel combination therapies with cisplatin, to increase efficacy and reduce its toxicity. Here, we screened 16 medicinal plant extracts from Northeast part of India and found that leaf extract of Zanthoxylum armatum DC. (ZALE) induced cytotoxicity as well as an effect on the increasing of the efficiency of chemotherapeutic drugs (cisplatin, mitomycin C and camptothecin). This work shows detail molecular mechanism of anti-cancer activity of ZALE and its potential for combined treatment regimens to enhance the apoptotic response of chemotherapeutic drugs. RESULTS: ZALE induced cytotoxicity, nuclear... |
| Tipo: Journal article |
Palavras-chave: Zanthoxylum armatum; Apoptosis; ERK activation; Cisplatin sensitization; DNA damage. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100046 |
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| Desmarchelier,C.; Coussio,J.; Ciccia,G.. |
| Achyrocline satureioides (Lam.) DC. (Compositae) is a medicinal herb used in Argentina, Uruguay, Brazil and Paraguay for its choleretic, antispasmodic and hepatoprotective properties. The presence of the flavonoid quercetin and its derivatives, and of different phenolic acids such as caffeic, chlorogenic and isochlorogenic acids in the aerial parts of this plant has led us to study the antioxidant activity of its extracts using different bioassays. The inhibition of luminol-enhanced chemiluminescence by the aqueous and methanolic extracts was used to show that their total reactive antioxidant potential index (TRAP; in µM Trolox equivalents) was 91.0 ± 15.4 and 128.1 ± 20.1 µM, respectively, while the total antioxidant reactivity index (TAR) was calculated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Achyrocline satureioides; Antioxidant activity; Luminol-enhanced chemiluminescence; Thiobarbituric acid-reactive substances; Hydroperoxide-initiated chemiluminescence; Lipid peroxidation; DNA damage; Reactive oxygen species. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900010 |
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| Pandir,Dilek. |
| ABSTRACT Organophosphorous pesticides (OPs) posses a great potential of acute toxicity for exposed animals and men. To evaluate the toxic potential of the organophosphate diazinon on root meristematic cells of Allium cepa L., was created two groups: In group 1 (control group), was not given any chemical. In group 2 (diazinon-treatment group), different doses (10, 40, 80 and 160 ppm) and times periods (24, 48 and 72 h) were administered. After exposure, cell death, effective concentration (EC50), mitotic index, cellular /chromosome aberrations, DNA damage by comet assay and RAPD-PCR were assessed at exposure times. EC50 value of diazinon was detected approximately 80 ppm. Hyperchromasia, later segragation, micronucleus, pulverised nucleus, nuclear... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diazinon; Allium test; DNA damage; Genotoxicty; RAPD-PCR. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100406 |
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| Pandir,Dilek. |
| ABSTRACTThe aim of this work was to assess the damage of DNA in human blood cell and spermin vitro under the influence of furan. These cells were administered 0-600 μM of furan at 37 and 32oC for 30 and 60 min, respectively. A significant increase in tail DNA%, tail length and moment indicating DNA damage was observed at increasing doses when compared to the controls. The treatment with 300 and 600 μM of furan showed a maximum increase of 86.74 ± 2.43 and 93.29 ± 8.68 compared to the control tail DNA% in lymphocytes. However, only 600 μM of furan showed a maximum increase of 94.71 ± 6.24 compared to the control tail DNA% in sperm. The results suggested that furan caused DNA damage in lymphocytes at increasing doses, but appeared to have not the same effect... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Furan; Tail length; DNA damage; Human blood cells; Comet assay. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500773 |
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| Bean, Tp; Akcha, Farida. |
| Single cell gel electrophoresis (SCGE or comet) assay allows quantification of DNA damage in individual cells and is an ideal tool for use within biological monitoring programmes. Comet assay can be used on a range of cell types including somatic, reproductive (gametes) or circulatory cells in many different species including both marine bivalves and flatfish. The assay can be employed with simple equipment available in most laboratories, is sensitive to environmentally relevant levels of DNA damage (Frenzilli and Lyons, 2009), accurately demonstrates a linear dose response to exposure (Collins et al., 1996), and can be adapted for use on most nucleated cell types. This document concentrates on the simplest and most repeatable method of comet assay in... |
| Tipo: Text |
Palavras-chave: Comet assay; Single cell gel electrophoresis; DNA damage; Genotoxicity; Biological effects; Bivalves; Flatfish; Integrated monitoring. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00587/69902/67807.pdf |
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| Zepeda,Andrea B.; Figueroa,Carolina A.; Abdalla,Dulcineia S.P.; Maranhão,Andrea Q.; Ulloa,Patricio H.; Pessoa Jr,Adalberto; Farías,Jorge G.. |
| Pichia pastoris is methylotrophic yeast used as an efficient expression system for heterologous protein production. In order to evaluate the effects of temperature (10 and 30 °C) and methanol (1 and 3% (v/v)) on genetically-modified Pichia pastoris, different biomarkers were evaluated: Heat stress (HSF-1 and Hsp70), oxidative stress (OGG1 and TBARS) and antioxidant (GLR). Three yeast cultures were performed: 3X = 3% methanol-10 °C, 4X = 3% methanol-30 °C, and 5X = 1% methanol-10°C. The expression level of HIF-1α, HSF-1, HSP-70 and HSP-90 biomarkers were measured by Western blot and in situ detection was performed by immunocytochemistry. Ours results show that at 3% methanol -30 °C there is an increase of mitochondrial OGG1 (mtOGG1), Glutathione Reductase... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oxidative stress; Recombinant yeast; Heat stress protein; DNA damage. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200014 |
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| Faisal,Mohammad; Saquib,Quaiser; Alatar,Abdulrahman A.; Al-Khedhairy,Abdulaziz A.; Ahmed,Mukhtar; Ansari,Sabiha M.; Alwathnani,Hend A.; Dwivedi,Sourabh; Musarrat,Javed; Praveen,Shelly. |
| BACKGROUND: Despite manifold benefits of nanoparticles (NPs), less information on the risks of NPs to human health and environment has been studied. Cobalt oxide nanoparticles (Co3O4-NPs) have been reported to cause toxicity in several organisms. In this study, we have investigated the role of Co3O4-NPs in inducing phytotoxicity, cellular DNA damage and apoptosis in eggplant (Solanum melongena L. cv. Violetta lunga 2). To the best of our knowledge, this is the first report on Co3O4-NPs showing phytotoxicity in eggplant. RESULTS: The data revealed that eggplant seeds treated with Co3O4-NPs for 2 h at a concentration of 1.0 mg/ml retarded root length by 81.5 % upon 7 days incubation in a moist chamber. Ultrastructural analysis by transmission electron... |
| Tipo: Journal article |
Palavras-chave: Cobalt oxide nanoparticles; Nanotoxicity; DNA damage; Apoptosis; Oxidative stress. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100020 |
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| Mendonça,Leonardo Meneghin; Machado,Carla da Silva; Teixeira,Cristiane Cardoso Correia; Freitas,Luis Alexandre Pedro de; Bianchi,Maria Lourdes Pires; Antunes,Lusânia Maria Greggi. |
| Abstract Curcumin (CMN) is the principal active component derived from the rhizome of Curcuma longa (Curcuma longa L.). It is a liposoluble polyphenolic compound that possesses great therapeutic potential. Its clinical application is, however, limited by the low concentrations detected following oral administration. One key strategy for improving the solubility and bioavailability of poorly water-soluble drugs is solid dispersion, though it is not known whether this technique might influence the pharmacological effects of CMN. Thus, in this study, we aimed to evaluate the antioxidant and antigenotoxic effects of CMN formulated in a solid dispersion (CMN SD) compared to unmodified CMN delivered to Wistar rats. Cisplatin (cDDP) was used as the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Curcuma longa; Antigenotoxicity; Micronucleus test; DNA damage; Comet assay. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400490 |
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| Alvarez-Moya,Carlos; Reynoso Silva,Mónica; Valdez Ramírez,Carlos; Gómez Gallardo,David; León Sánchez,Rafael; Canales Aguirre,Alejandro; Feria Velasco,Alfredo. |
| There is considerable controversy with regard to the genotoxicity of glyphosate, with some reports stating that this compound is non-toxic for fish, birds and mammals. In this work, we used the comet assay to examine the genotoxicity of glyphosate isopropylamine (0.7, 7, 70 and 700 µM) in human lymphocytes, erythrocytes of Oreochromis niloticus and staminal nuclei of Tradescantia (4430) in vitro and in vivo. Cells, nuclei and fish that had and had not been exposed to 5 mM N-nitrosodiethylamine (NDEA) were used as positive and negative controls, respectively. Significant (p < 0.01) genetic damage was observed in vivo and in vitro in all cell types and organisms tested. Human lymphocytes and Tradescantia hairs showed lower genetic damage in vivo compared... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Comet assay; DNA damage; Genotoxicity; Glyphosate. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100016 |
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| Kumar,Namrata; Moreno,Natália C.; Feltes,Bruno C.; Menck,Carlos FM; Houten,Bennett Van. |
| Abstract Base and nucleotide excision repair (BER and NER) pathways are normally associated with removal of specific types of DNA damage: small base modifications (such as those induced by DNA oxidation) and bulky DNA lesions (such as those induced by ultraviolet or chemical carcinogens), respectively. However, growing evidence indicates that this scenario is much more complex and these pathways exchange proteins and cooperate with each other in the repair of specific lesions. In this review, we highlight studies discussing the involvement of NER in the repair of DNA damage induced by oxidative stress, and BER participating in the removal of bulky adducts on DNA. Adding to this complexity, UVA light experiments revealed that oxidative stress also causes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Base excision repair; Nucleotide excision repair; DNA damage; Protein oxidation; UVA light. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200309 |
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| Tousson,Ehab; El-Atrsh,Afaf; Mansour,Merfaat; Assem,Abdallah. |
| Ethephon (Ethrel®) is an ethylene-based plant growth regulator that used in agriculture and it has direct and indirect effects on human health, direct effect via its inhalation during usage in agriculture and indirect effect through the diet (Fruits and vegetables) that is sprayed with it. The current study aimed to examine the possible modifying effects of costus (Saussurea lappa) root aqueous extract against Ethephon induced liver toxicity, injury, DNA fragmentation and PCNA alterations in male rats. Fifty adult male rats were divided into 5 groups (1st, control; 2nd, Costus; 3rd, Ethephon; 4th, Post treated Ethephon with costus; 5th, self-healing Ethephon). Current results revealed that; a significant increase in aspartate transaminase (AST), alanine... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant growth regulator; Ethephon; Costus; Liver; DNA damage; PCNA expression; Rat. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100600 |
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| SCHNEIDER,MARCIA R.; SILVA,ANDRÉA L.G. DA; GOULART,CÁSSIA L.; SCHNEIDERS,PALOMA B.; WEBER,AUGUSTO F.; POSSUELO,LIA G.; VALIM,ANDREIA R.M.. |
| Abstract: This study evaluated 24 patients with lung cancer (CA) and 23 individuals with no smoking history or cancer in the family and without respiratory disease in childhood (CO). Peripheral blood lymphocytes was used to perform alkaline comet assay and to assess DNA damage as well as to evaluate methyl methane sulfonate (MMS) DNA repair after one hour and three hours at 37 ºC. The percentage of residual damage (RD) after three hours of MMS treatment, for each patient was assessed. The majority of patients were in the CA group, male patients, former smokers, with a history of smoking for 15 years and without associated comorbidities. Alkaline and residual damages were higher in the CA group when compared to controls (alkaline damage P = 0.015 and RD P =... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Comet assay; DNA damage; DNA repair; Lung cancer. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500806 |
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| STERPONE,SILVIA; CORNETTA,TOMMASO; ANGIONI,ADRIANO; FISCARELLI,ERSILIA; LUCIDI,VINCENZINA; TESTA,ANTONELLA; COZZI,RENATA. |
| Cystic Fibrosis (CF) is an autosomal recessive multisystemic disorder showing a highly heterogeneous phenotype, even among siblings carrying identical CFTR mutations. Moreover, oxidative stress is of central importance in the pathogenesis of cystic fibrosis. The present study seeks to value the presence of oxidative damage in CF patients and the possible modifier effect of repair and glutathione-S-transferase genes. We analysed the presence of DNA damage in leukocytes of 63 CF patients at an Italian CF centre and 63 controls, through the alkaline Comet assay to detect DNA strand breaks. Furthermore, controls and 93 CF subjects were genotyped for 5 genes by RFLP-PCR (XRCC1,0GG1,GSTP1) and PCR assay (GSTM1, GSTT1). No difference in Comet assay values was... |
| Tipo: Journal article |
Palavras-chave: Comet assay; Cystic fibrosis; DNA damage; Genetic polymorphisms; Modifier genes. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400009 |
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| Registros recuperados: 50 | |
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