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Registros recuperados: 35
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Absence of CpG observed/expected ratio depletion in Haemonchus contortus evidences a non-methylated genome. Repositório Alice
NICIURA, S. C. M.; THOLON, P..
Editores: Luiz Lehmann Coutinho, ESALQ/USP; Luciana Correia de Almeida Regitano, Embrapa Pecuária Sudeste; Gerson Barreto Mourão, ESALQ/USP; Aline Silva Mello Cesar, ESALQ/USP; Bárbara Silva Vignato, FZEA/USP; Mirele Daiana Poleti, ESALQ/USP; Wellison Jarles da Silva Diniz, UFSCar.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Epigenetics; DNA methylation; Gastrointestinal nematode; Barber?s pole worm.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1075655
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Age-related Changes in DNA Methylation Status of hTERT Gene Promoter of Oral Epithelial Cells BABT
Bezerra,Stephane Flaviane de Oliveira; Costa,Ludimila de Araújo; Freitas,Priscylla Alves Nascimento de; Oliveira,Naila Francis Paulo de.
The purpose of this study was to investigate the effect of aging on the DNA methylation status of two genes involved in tumorigenesis (telomerase gene hTERT and DNA repair gene- MLH1) and one in metabolism (methylenetetrahydrofolate reductase gene- MTHFR) in oral epithelial cells. DNA methylation analysis was performed by Methylation Sensitive Restriction Enzymes (MSRE) of healthy oral epithelial cells of child (6-10 years, n=21), young (20-25 years, n=19) and elderly (over 60 years, n=25). The results for the hTERT gene showed significant variation in the methylation frequency at CpG dinucleotides among the groups (p=0.0001), with the methylated condition more frequently in children and young people. In relation to MLH1 and MTHFR, no differences were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epigenetic; DNA methylation; Aging; DNA repair; Telomerase; MTHFR.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100082
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Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker Electron. J. Biotechnol.
Tiwari,Jagesh Kumar; Saurabh,Shaumaya; Chandel,Poonam; Singh,Bir Pal; Bhardwaj,Vinay.
Background: Genetic and epigenetic changes (DNA methylation) were examined in the tissue-culture propagated interspecific potato somatic hybrids between dihaploid Solanum tuberosum and S. pinnatisectum. Amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified polymorphism (MSAP) were applied to detect the genetic and epigenetic changes, respectively in the somatic hybrids mother plants (1st cycle) and their regenerants (30th cycles sub-cultured). Results: To detect genetic changes, eight AFLP primer combinations yielded a total of 329 scorable bands of which 49 bands were polymorphic in both mother plants and regenerants. None of the scorable bands were observed in term of loss of original band of mother plant or gain of novel band...
Tipo: Journal article Palavras-chave: AFLP; DNA methylation; In vitro propagation; MSAP; Potato; Somatic hybrids.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600005
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Biomarkers towards Ovarian Cancer Diagnostics: Present and Future Prospects BABT
Rastogi,Meenal; Gupta,Sameer; Sachan,Manisha.
ABSTRACT Ovarian carcinoma accounts for highest mortality of all gynecologic malignancies as the disease is asymptomatic until late stages. Biomarkers such as CA-125 and HE4 are being currently used for diagnosis of ovarian cancer, but they show contradicting diagnostic accuracy. Therefore other biomarkers have been investigated for early detection of this disease, but no success has been obtained and no test has yet been recommended for screening a general population. In this instance, aptamers can be effectively used to identify tumor-specific antigens for early diagnosis and targeted therapy of ovarian cancer. This article provides an overview of the biomarkers/panels being explored as well as the potential of aptamers to improve current long-term...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovarian cancer; Aptamers; DNA methylation; CA-125; HE4.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100346
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Characterisation of the methylation pattern in the intragenic CpG island of the IGF2 gene in Bos taurus indicus cumulus cells during in vitro maturation. Repositório Alice
FRANCO, M. M.; FAGUNDES, N. S.; MICHALCZECHEN-LACERDA, V. A.; CAIXETA, E. S.; RODRIGUES, F. de C.; MACHADO, G. M.; FERREIRA, A. R.; DODE, M. A. N..
bitstream/item/180017/1/Franco2014-Article-CharacterisationOfTheMethylati.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cumulus cells; IGF2; In vitromaturation; Bovine; DNA methylation; Epigenetics.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/995151
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Copper induces expression and methylation changes of early development genes in Crassostrea gigas embryos ArchiMer
Sussarellu, Rossana; Lebreton, Morgane; Rouxel, Julien; Akcha, Farida; Riviere, Guillaume.
Copper contamination is widespread along coastal areas and exerts adverse effects on marine organisms such as mollusks. In the Pacific oyster, copper induces severe developmental abnormalities during early life stages; however, the underlying molecular mechanisms are largely unknown. This study aims to better understand whether the embryotoxic effects of copper in Crassostrea gigas could be mediated by alterations in gene expression, and the putative role of DNA methylation, which is known to contribute to gene regulation in early embryo development. For that purpose, oyster embryos were exposed to 4 nominal copper concentrations (0.1, 1, 10 and 20 μg L−1 Cu2+) during early development assays. Embryotoxicity was monitored through the oyster embryo-larval...
Tipo: Text Palavras-chave: Oyster; Embryotoxicity; Copper; Gene expression; DNA methylation.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00417/52856/53756.pdf
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Correlations of recognition memory performance with expression and methylation of brain-derived neurotrophic factor in rats Biol. Res.
Muñoz,Pablo C; Aspé,Mauricio A; Contreras,Luis S; Palacios,Adrián G.
Object recognition memory allows discrimination between novel and familiar objects. This kind of memory consists of two components: recollection, which depends on the hippocampus, and familiarity, which depends on the perirhinal cortex (Pcx). The importance of brain-derived neurotrophic factor (BDNF) for recognition memory has already been recognized. Recent evidence suggests that DNA methylation regulates the expression of BDNF and memory. Behavioral and molecular approaches were used to understand the potential contribution of DNA methylation to recognition memory. To that end, rats were tested for their ability to distinguish novel from familiar objects by using a spontaneous object recognition task. Furthermore, the level of DNA methylation was...
Tipo: Journal article Palavras-chave: DNA methylation; Recognition memory; Synaptic plasticity; Hippocampal; Perirhinal cortex; BDNF.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200013
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Cryptic mosaicism involving a second chromosome X in patients with Turner syndrome BJMBR
Araújo,A.; Ramos,E.S..
The high abortion rate of 45,X embryos indicates that patients with Turner syndrome and 45,X karyotype could be mosaics, in at least one phase of embryo development or cellular lineage, due to the need for the other sex chromosome presence for conceptus to be compatible with life. In cases of structural chromosomal aberrations or hidden mosaicism, conventional cytogenetic techniques can be ineffective and molecular investigation is indicated. Two hundred and fifty patients with Turner syndrome stigmata were studied and 36 who had female genitalia and had been cytogenetically diagnosed as having "pure" 45,X karyotype were selected after 100 metaphases were analyzed in order to exclude mosaicism and the presence of genomic Y-specific sequences (SRY, TSPY,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Turner syndrome; X-inactivation; HUMARA assay; DNA methylation; Criptic mosaicism.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500004
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Cucumber breeding and genomics: Potential from research with Cucumis hystrix National Institute of Agronomic Research
Jin-Feng Chen; Long-Zheng Chen; Yong Zhuang; You-Gen Chen; Xiao-Hui Zhou.
Cucumis hystrix Chakr. (2n=2x=24) is a wild Cucumis species found in Yunnan (China) having cross compatibility with cultivated cucumber (C. sativus L., 2n=2x=14). Since the first interspecific hybridization successfully made in 1995 and development of the synthetic allotetraploid species (C. X hytivus Chen and Kirkbride, 2n=4x=38) in 2000, experiments with C. hystrix and the progenies derived from this interspecific cross were carried out for the potentials in cucumber genetics and cultivar improvements. Some important characters or traits found in C. hystrix were introduced, and the novel germplasm developed from this cross and some results from genetic and epigenetic studies were summarized for the first time in this review. Future objectives are...
Tipo: Conference Paper Palavras-chave: Cucumis; Cucumber; Interspecific hybridization; Introgression; Wild species; Germplasm; Disease resistance; Photosynthesis; Genome; DNA methylation; Molecular markers.
Ano: 2008 URL: http://hdl.handle.net/2174/200
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Cytogenetic evidences of genome rearrangement and differential epigenetic chromatin modification in the sea lamprey (Petromyzon marinus) ArchiMer
Covelo-soto, Lara; Moran, Paloma; Pasantes, Juan J.; Perez Garcia, Maria Concepcion.
This work explores both the chromatin loss and the differential genome methylation in the sea lamprey (Petromyzon marinus) from a molecular cytogenetic point of view. Fluorescent in situ hybridization experiments on meiotic bivalents and mitotic chromosomes corroborate the chromatin loss previously observed during the development of the sea lamprey and demonstrate that the elimination affects not only to Germ1 sequences but also to the rpt200 satellite DNA and most part of the major ribosomal DNA present on the germinal line. 5-Methylcytosine immunolocation revealed that the GC-rich heterochromatin is highly methylated in the germ line but significantly less in somatic chromosomes. These findings not only support previous observations about genome...
Tipo: Text Palavras-chave: Sea lamprey; Chromatin loss; Genome rearrangements; DNA methylation.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00247/35811/34327.pdf
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Developmental Methylome of the Medicinal Plant Catharanthus roseus Unravels the Tissue-Specific Control of the Monoterpene Indole Alkaloid Pathway by DNA Methylation ArchiMer
Dugé De Bernonville, Thomas; Maury, Stéphane; Delaunay, Alain; Daviaud, Christian; Chaparro, Cristian; Tost, Jörg; O’connor, Sarah Ellen; Courdavault, Vincent.
Catharanthus roseus produces a wide spectrum of monoterpene indole alkaloids (MIAs). MIA biosynthesis requires a tightly coordinated pathway involving more than 30 enzymatic steps that are spatio-temporally and environmentally regulated so that some MIAs specifically accumulate in restricted plant parts. The first regulatory layer involves a complex network of transcription factors from the basic Helix Loop Helix (bHLH) or AP2 families. In the present manuscript, we investigated whether an additional epigenetic layer could control the organ-, developmental- and environmental-specificity of MIA accumulation. We used Whole-Genome Bisulfite Sequencing (WGBS) together with RNA-seq to identify differentially methylated and expressed genes among nine samples...
Tipo: Text Palavras-chave: Alkaloids; DNA methylation; Epigenetics; Folivory; Organs; Plant specialized metabolism.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00652/76453/77543.pdf
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DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients Genet. Mol. Biol.
Lima,Patrícia Santos Pereira; Molffeta,Greice Andreoti; Araujo,Amélia Góes de; Zago,Marco Antônio; Silva Jr.,Wilson Araújo da.
The aim of this work was to evaluate the methylation profile of the p15 (CDKN2B) gene in Brazilian patients with leukemia and to correlate the CDKN2B gene expression with the percentage of methylated CpG dinucleotides in its promoter region. Thirty-one samples from six patients with acute lymphocytic leukemia (ALL), four with chronic myeloid leukemia (CML), and 21 with acute myeloid leukemia (AML) were evaluated by MSP (Methylation-Specific PCR). The CDKN2B gene was found to be methylated in four (67%) of the six ALL samples and in 16 (76%) of the 21 AML samples, but in none of the four CML samples analyzed. We observed a correlation between the CDKN2B mRNA expression (RT-PCR) and the percentage of methylated CpG dinucleotides. Therefore, this study in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CpG island; DNA methylation; CDKN2B.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400005
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DNA methylation and functional characterization of the XIST gene during in vitro early embryo development in cattle. Repositório Alice
MENDONÇA, A. dos S.; SILVEIRA, M. M.; RIOS, A. F. L.; MANGIAVACCHI, P. M.; CAETANO, A. R.; DODE, M. A. N.; FRANCO, M. M..
bitstream/item/201952/1/DNA-methylation-and-functional-characterization-of-the-XIST-gene-during-in-vitro-early-embryo-development-in-cattle.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: X chromosome inactivation; XIST; DNA methylation; Gene expression; Genomics.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112182
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DNA methylation modulates H19 and IGF2 expression in porcine female eye Genet. Mol. Biol.
Wang,Dongxu; Wang,Guodong; Yang,Hao; Liu,Haibo; Li,Cuie; Li,Xiaolan; Lin,Chao; Song,Yuning; Li,Zhanjun; Liu,Dianfeng.
Abstract The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively). We observed a normal monoallelic methylation pattern for H19 differentially methylated regions (DMRs). Compared with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: H19/IGF2; Gene expression; DNA methylation; Parthenogenetic; Pig.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100153
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DNA methylation of the insulin-like growth factor 2-Imprinted gene in trophoblast cells of elongated bovine embryo: effects of the in vitro culture. Repositório Alice
MENDONÇA, A. dos S.; FRANCO, M. M.; CARVALHO, J. de O.; MACHADO, G. M.; DODE, M. A. N..
bitstream/item/203963/1/cell.2019.0041.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Imprinting; Posthatching culture; Cattle; DNA methylation.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113678
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Dynamic epimarks in sex-related genes predict gonad phenotype in the European sea bass, a fish with mixed genetic and environmental sex determination ArchiMer
Anastasiadi, Dafni; Vandeputte, Marc; Sanchez-baizan, Nuria; Allal, Francois; Piferrer, Francesc.
The integration of genomic and environmental influences into methylation patterns to bring about a phenotype is of central interest in developmental epigenetics, but many details are still unclear. The sex ratios of the species used here, the European sea bass, are determined by genetic and temperature influences. We created four families from parents known to produce offspring with different sex ratios, exposed larvae to masculinizing temperatures and examined, in juvenile gonads, the DNA methylation of seven genes related to sexual development by a targeted sequencing approach. The genes most affected by both genetics and environment were cyp19a1a and dmrt1, with contrasting sex-specific methylation and temperature responses. The relationship between...
Tipo: Text Palavras-chave: Early development; Environmental temperature; DNA methylation; Sex determination; Aromatase; Cyp19a1a; Dmrt1; Epigenetic marks; DNA methylation threshold; Epigenetic inheritance.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00470/58123/61657.pdf
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Effect of nickel chloride on Arabidopsis genomic DNA and methylation of 18S rDNA Electron. J. Biotechnol.
Li,Zhongai; Chen,Xin; Li,Suoping; Wang,Zicheng.
Background In recent years, nickel (Ni) has been widely applied in industrial and agricultural production and has become a kind of environmental pollution. In this study, the effect of nickel chloride (NiCl2) with different concentrations on Arabidopsis genomic stability and DNA methylation has been demonstrated. The nucleolus variation and 18S rDNA methylation after NiCl2 treatment have been analyzed. Results The results are as follows: (1) The NiCl2 could result in heritable genomic methylation variations. The genomic DNA methylation variations have been detected by methylation-sensitive amplified polymorphism (MSAP) molecular markers, and the result showed that after NiCl2 treatment, there was methylation variation in T0 generation seedlings, and...
Tipo: Journal article Palavras-chave: DNA methylation; Heavy metal; Methylation sensitive amplified polymorphism (MSAP); Nickel.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100010
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Effects of a parental exposure to diuron on Pacific oyster spat methylome ArchiMer
Rondon Sallan, Rodolfo; Grunau, Christoph; Fallet, Manon; Charlemagne, Nicolas; Sussarellu, Rossana; Chaparro, Cristian; Montagnani, Caroline; Mitta, Guillaume; Bachere, Evelyne; Akcha, Farida; Cosseau, Celine.
Environmental epigenetic is an emerging field that studies the cause-effect relationship between environmental factors and heritable trait via an alteration in epigenetic marks. This field has received much attentions since the impact of environmental factors on different epigenetic marks have been shown to be associated with a broad range of phenotypic disorders in natural ecosystems. Chemical pollutants have been shown to affect immediate epigenetic information carriers of several aquatic species but the heritability of the chromatin marks and the consequences for long term adaptation remain open questions. In this work, we investigated the impact of the diuron herbicide on the DNA methylation pattern of spat from exposed Crassotrea gigas genitors. This...
Tipo: Text Palavras-chave: Crassostrea gigas; Mollusk; DNA methylation; Herbicide; Epigenetic.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00602/71413/69895.pdf
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Environmentally Driven Color Variation in the Pearl Oyster Pinctada margaritifera var. cumingii (Linnaeus, 1758) Is Associated With Differential Methylation of CpGs in Pigment- and Biomineralization-Related Genes ArchiMer
Stenger, Pierre-louis; Ky, Chin-long; Reisser, Celine; Cosseau, Céline; Grunau, Christoph; Mege, Mickael; Planes, Serge; Vidal-dupiol, Jeremie.
Today, it is common knowledge that environmental factors can change the color of many animals. Studies have shown that the molecular mechanisms underlying such modifications could involve epigenetic factors. Since 2013, the pearl oyster Pinctada margaritifera var. cumingii has become a biological model for questions on color expression and variation in Mollusca. A previous study reported color plasticity in response to water depth variation, specifically a general darkening of the nacre color at greater depth. However, the molecular mechanisms behind this plasticity are still unknown. In this paper, we investigate the possible implication of epigenetic factors controlling shell color variation through a depth variation experiment associated with a DNA...
Tipo: Text Palavras-chave: Pearl oyster; Environmental pressure; Depth; Color change; Pigmentation; DNA methylation; Methylome characterization.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00685/79667/82436.pdf
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Epigenetic modifications and their relation to caste and sex determination and adult division of labor in the stingless bee Melipona scutellaris Genet. Mol. Biol.
Cardoso-Júnior,Carlos A.M.; Fujimura,Patrícia Tieme; Santos-Júnior,Célio Dias; Borges,Naiara Araújo; Ueira-Vieira,Carlos; Hartfelder,Klaus; Goulart,Luiz Ricardo; Bonetti,Ana Maria.
Abstract Stingless bees of the genus Melipona, have long been considered an enigmatic case among social insects for their mode of caste determination, where in addition to larval food type and quantity, the genotype also has a saying, as proposed over 50 years ago by Warwick E. Kerr. Several attempts have since tried to test his Mendelian two-loci/two-alleles segregation hypothesis, but only recently a single gene crucial for sex determination in bees was evidenced to be sex-specifically spliced and also caste-specifically expressed in a Melipona species. Since alternative splicing is frequently associated with epigenetic marks, and the epigenetic status plays a major role in setting the caste phenotype in the honey bee, we investigated here epigenetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA methylation; Histone modification; Caste development; Social bees; Genetic caste determination.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100061
Registros recuperados: 35
Primeira ... 12 ... Última
 

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