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| Registros recuperados: 371 | |
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| Burgdorf,R.J.; Laing,M.D.; Morris,C.D.; Jamal-Ally,S.F.. |
| Extraneous DNA interferes with PCR studies of endophytic fungi. A procedure was developed with which to evaluate the removal of extraneous DNA. Wheat (Triticum aestivum) leaves were sprayed with Saccharomyces cerevisiae and then subjected to physical and chemical surface treatments. The fungal ITS1 products were amplified from whole tissue DNA extractions. ANOVA was performed on the DNA bands representing S. cerevisiae on the agarose gel. Band profile comparisons using permutational multivariate ANOVA (PERMANOVA) and non-metric multidimensional scaling (NMDS) were performed on DGGE gel data, and band numbers were compared between treatments. Leaf surfaces were viewed under variable pressure scanning electron microscopy (VPSEM). Yeast band analysis of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endophyte; Fungi; DNA; Surface sterilization. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300030 |
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| Strong, Ellen E.; Bouchet, Philippe. |
| A new genus, Limatium gen, n., and two new species, L. pagodula sp, n, and L. aureum sp, n. are described, found on outer slopes of barrier reefs and fringing reefs in the South Pacific. They are rare for cerithiids, which typically occur in large populations. The two new species arc represented by 108 specimens sampled over a period of 30 years, only 16 of which were collected alive. Three subadults from the Philippines and Vanuatu likely represent a third species. In addition to their rarity, Limatium species arc atypical for cerithiids in their smooth, polished, honey to golden brown shells with distinctive white fascioles extending suture to suture. The radula presents a unique morphology that does not readily suggest an affinity to any of the... |
| Tipo: Text |
Palavras-chave: Bittiinae; New genus; New species; Marine; DNA. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00494/60615/64101.pdf |
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| Bouga, M.; ALAUX, C.; Bienkowska, M.; Büchler, R.; Carreck, N.L.; Cauia, E.; Chlebo, R.; Dahle, B.; Dall'Olio, R.; De la Rúa, P.; Gregorc, A.; Ivanova, E.; Kence, A.; Kence, M.; Kezic, N.; Kiprijanovska, H.; Kozmus, P.; Kryge, P.; Le Conte, Y.; Lodesani, M.; Murilhas, A.M.; Siceanu, A.; Soland, G.; Uzunov, A.; Wilde, J.. |
| Here, scientists from 19 European countries, most of them collaborating in Working Group 4: “Diversity and Vitality” of COST Action FA 0803“Prevention of honey bee COlony LOSSes” (COLOSS), review the methodology applied in each country for discriminating between honey beepopulations. Morphometric analyses (classical and geometric) and different molecular markers have been applied. Even if the approach hasbeen similar, however, different methodologies regarding measurements, landmarks or molecular markers may have been used, as well asdifferent statistical procedures. There is therefore the necessity to establish common methods in all countries in order to have results that canbe directly compared. This is one of the goals of WG4 of the COLOSS project |
| Tipo: Journal Article |
Palavras-chave: ABEILLE DOMESTIQUE; INSECTE SOCIAL; APICULTURE; SOUS-ESPECE; MORPHOMETRIE; ADN; ISOENZYME; MARQUEUR MOLECULAIRE; METHODOLOGIE; SYSTEMATIQUE; DIAGNOSE MOLECULAR MARKER; METHODOLOGY; SYSTEMATICS; DIAGNOSIS; HONEYBEE; BEEKEEPING; SUB-SPECIES; MORPHOMETRICS; DNA; ISOENZYMES. |
| Ano: 2011 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011a0114f97&uri=/notices/prodinra1/2011/04/ |
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| Moraes-Barros,Nadia de; Morgante,João Stenghel. |
| Museum collections have been widely used as sources of biological samples for molecular biology studies and there are several methodologies and techniques to obtain and analyze DNA from tissues archived in museums, but most of these protocols have been developed for a specific tissue or are commercial kits. We present a simple protocol for extracting and amplifying DNA segments from sloth museum specimens. With this simple protocol we analyzed DNA fragments from 64% of 64 skin samples from three-toed sloths (Bradypus variegatus and Bradypus tridactylus) archived in three different museums: 43 samples from the University of São Paulo Museum of Zoology (Museu de Zoologia da Universidade de São Paulo, MUZUSP) São Paulo, São Paulo, Brazil; 18 samples from the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bradypus; DNA; Dried skins; Museum specimens. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600024 |
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| LIMA, R. S. N.; SANTOS, C. A. F.; RODRIGUES, M. A.; BATISTA, P. P.. |
| Diversas técnicas de biologia molecular estão disponíveis para a detecção da variabilidade genética ao nível de seqüência de DNA. O objetivo do presente trabalho foi ajustar a técnica de extração de DNA a partir de diferentes concentrações de -mercaptoetanol, utilizando o protocolo CTAB 2X. DNA genômico total foi isolado de folhas verdes e sadias em espécies distintas como umbuzeiro, mangueira, cebola e goiabeira, segundo protocolo do CTAB 2X com algumas adaptações (500 mM Tris pH 8,0; 1,4 M NaCl; CTAB 0,2% (p/v); 2% -mercaptoetanol; 20 mM de EDTA). A concentração do mercaptoetanol utilizado foi de 2%, um aumento de 10X em relação ao protocolo padrão. A concentração e a integridade do DNA genômico foram observadas em géis 0.8% de agarose comum, comparado a... |
| Tipo: Artigo em anais de congresso (ALICE) |
Palavras-chave: Extração; DNA; Espécie vegetal; Protocolo. |
| Ano: 2007 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/160442 |
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| NUNES, C. F.; FERREIRA, J. L.; FERNANDES, M. C. N.; BREVES, S. de S.; GENEROSO, A. L.; SOARES, B. D. F.; DIAS, M. S. C.; PASQUAL, M.; BOREM, A.; CANÇADO, G. M. de A.. |
| 2011 |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Fragaria ananassa; DNA; Liofilização; Morango; PCR. |
| Ano: 2011 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/955705 |
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| Registros recuperados: 371 | |
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