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Registros recuperados: 194
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A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel BJM
Qiu,Shanlian; Chen,Jichen; Lin,Si; Lin,Xinjian.
Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Backed gel; DNA; Silver staining.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200029
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A procedure to evaluate the efficiency of surface sterilization methods in culture-independent fungal endophyte studies BJM
Burgdorf,R.J.; Laing,M.D.; Morris,C.D.; Jamal-Ally,S.F..
Extraneous DNA interferes with PCR studies of endophytic fungi. A procedure was developed with which to evaluate the removal of extraneous DNA. Wheat (Triticum aestivum) leaves were sprayed with Saccharomyces cerevisiae and then subjected to physical and chemical surface treatments. The fungal ITS1 products were amplified from whole tissue DNA extractions. ANOVA was performed on the DNA bands representing S. cerevisiae on the agarose gel. Band profile comparisons using permutational multivariate ANOVA (PERMANOVA) and non-metric multidimensional scaling (NMDS) were performed on DGGE gel data, and band numbers were compared between treatments. Leaf surfaces were viewed under variable pressure scanning electron microscopy (VPSEM). Yeast band analysis of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophyte; Fungi; DNA; Surface sterilization.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300030
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A rare and unusual new bittiine genus with two new species from the South Pacific (Cerithiidae, Gastropoda) ArchiMer
Strong, Ellen E.; Bouchet, Philippe.
A new genus, Limatium gen, n., and two new species, L. pagodula sp, n, and L. aureum sp, n. are described, found on outer slopes of barrier reefs and fringing reefs in the South Pacific. They are rare for cerithiids, which typically occur in large populations. The two new species arc represented by 108 specimens sampled over a period of 30 years, only 16 of which were collected alive. Three subadults from the Philippines and Vanuatu likely represent a third species. In addition to their rarity, Limatium species arc atypical for cerithiids in their smooth, polished, honey to golden brown shells with distinctive white fascioles extending suture to suture. The radula presents a unique morphology that does not readily suggest an affinity to any of the...
Tipo: Text Palavras-chave: Bittiinae; New genus; New species; Marine; DNA.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00494/60615/64101.pdf
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A review of methods for discrimination of honey bee populations as applied to European beekeeping Inra
Bouga, M.; ALAUX, C.; Bienkowska, M.; Büchler, R.; Carreck, N.L.; Cauia, E.; Chlebo, R.; Dahle, B.; Dall'Olio, R.; De la Rúa, P.; Gregorc, A.; Ivanova, E.; Kence, A.; Kence, M.; Kezic, N.; Kiprijanovska, H.; Kozmus, P.; Kryge, P.; Le Conte, Y.; Lodesani, M.; Murilhas, A.M.; Siceanu, A.; Soland, G.; Uzunov, A.; Wilde, J..
Here, scientists from 19 European countries, most of them collaborating in Working Group 4: “Diversity and Vitality” of COST Action FA 0803“Prevention of honey bee COlony LOSSes” (COLOSS), review the methodology applied in each country for discriminating between honey beepopulations. Morphometric analyses (classical and geometric) and different molecular markers have been applied. Even if the approach hasbeen similar, however, different methodologies regarding measurements, landmarks or molecular markers may have been used, as well asdifferent statistical procedures. There is therefore the necessity to establish common methods in all countries in order to have results that canbe directly compared. This is one of the goals of WG4 of the COLOSS project
Tipo: Journal Article Palavras-chave: ABEILLE DOMESTIQUE; INSECTE SOCIAL; APICULTURE; SOUS-ESPECE; MORPHOMETRIE; ADN; ISOENZYME; MARQUEUR MOLECULAIRE; METHODOLOGIE; SYSTEMATIQUE; DIAGNOSE MOLECULAR MARKER; METHODOLOGY; SYSTEMATICS; DIAGNOSIS; HONEYBEE; BEEKEEPING; SUB-SPECIES; MORPHOMETRICS; DNA; ISOENZYMES.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011a0114f97&uri=/notices/prodinra1/2011/04/
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A simple method for DNA isolation from Xanthomonas spp. Scientia Agricola
Gomes,Luiz Humberto; Duarte,Keila Maria Roncato; Andrino,Felipe Gabriel; Tavares,Flavio Cesar Almeida.
A simple DNA isolation method was developed with routine chemicals that yields high quality and integrity preparations when compared to some of the most well known protocols. The method described does not require the use of lysing enzymes, water bath and the DNA was obtained within 40 minutes The amount of nucleic acid extracted (measured in terms of absorbancy at 260 nm) from strains of Xanthomonas spp., Pseudomonas spp. and Erwinia spp. was two to five times higher than that of the most commonly used method.
Tipo: Info:eu-repo/semantics/report Palavras-chave: DNA; Xanthomonas; RAPD.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000300028
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A simple protocol for the extraction and sequence analysis of DNA from study skin of museum collections Genet. Mol. Biol.
Moraes-Barros,Nadia de; Morgante,João Stenghel.
Museum collections have been widely used as sources of biological samples for molecular biology studies and there are several methodologies and techniques to obtain and analyze DNA from tissues archived in museums, but most of these protocols have been developed for a specific tissue or are commercial kits. We present a simple protocol for extracting and amplifying DNA segments from sloth museum specimens. With this simple protocol we analyzed DNA fragments from 64% of 64 skin samples from three-toed sloths (Bradypus variegatus and Bradypus tridactylus) archived in three different museums: 43 samples from the University of São Paulo Museum of Zoology (Museu de Zoologia da Universidade de São Paulo, MUZUSP) São Paulo, São Paulo, Brazil; 18 samples from the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bradypus; DNA; Dried skins; Museum specimens.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600024
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A simplified DNA extraction method for PCR analysis of Camarotella spp. BABT
Vitória,Nadja Santos; Bezerra,José Luiz; Gramacho,Karina Peres.
This work aimed to optimize an efficient and simple protocol for DNA extraction of Camarotella species, an obligate plant pathogen that cause verrucosis or "lixa" on coconut tree and other palms, facilitating the molecular studies of these biotrophic microorganisms. The method proposed enabled a fast, reproducible and reliable DNA extraction from Camarotella species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Camarotella torrendiella; C. acrocomiae; Verrucosis on coconut; DNA; PCR.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200001
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Advantages of the metagenomic approach for soil exploration: reply from Vogel et al. Inra
Vogel, T.M.; Hirsch, P.R.; Simonet, P.; Janson, J.K.; Tiedje, J.M.; van Elsas, J.D.; Nalin, R.; Philippot, L.; Bailey, M.J..
Tipo: Journal Article Palavras-chave: MICROBIOLOGY; SOIL; ADVANTAGE; METAGENOMIC; DNA; ENVIRONMENT.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20109aa7b47b&uri=/notices/prodinra1/2011/06/
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Alterações de cromatina em espermatozóides de ovinos e caprinos avaliadas por azul de toluidina e alaranjado de acridina Ciência e Agrotecnologia
Kamimura,Cristina de Figueiredo; Jacomini,José Octávio; Beletti,Marcelo Emílio.
Reprodutores com espermograma normal podem se comportar como subférteis ou passarem por períodos de subfertilidade. As alterações na cromatina dos espermatozóides são possíveis explicações encontradas para tais comportamentos. Conduziu-se este trabalho, com o objetivo de testar a eficiência de azul de toluidina (AT) e do alaranjado de acridina (AA) na identificação de alterações na compactação de cromatina em espermatozóides de ovinos e caprinos, além de avaliar a correlação entre essas alterações e as de morfologia espermática. Para tal, foram avaliadas amostras de sêmen de 15 ovinos e de 15 caprinos, com dez repetições para cada método por animal. Calcularam-se a média, o desvio padrão (DP) e o coeficiente de variação (CV) para cada técnica e animal....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azul de toluidina; Alaranjado de acridina; Espermatozóide; Cromatina; DNA.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000100027
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Alterations in Biomarkers Associated with Sterility in Pectinophora gossypiella (Saunders) Induced by Gamma Irradiation BABT
Ali,Hanaa Mahmoud; Moustafa,Hemat Zakaria; Sayed,Rehab Mahmoud.
ABSTRACT Nowadays, radiation technology is widely used to produce changes in Biosystems. The goal of this work is to determine the variation induced male Pectinophora gossypiella in gamma-irradiated as pupae using 50Gy and 150Gy. Comparing elements composition and DNA (using RAPD-PCR) between substerile 50Gy and the sterile dose 150Gy in P. gossypiella showed variation between them. Potassium (K) was the most abundant elements in unirradiated and irradiated males followed by magnesium (Mg). The percentage of heavy metals as copper, zinc, and cadmium concurrent with K was directly proportional to the radiation dose. While the percentage of Mg, Phosphorous and calcium decreased as the radiation dose increased. The results also revealed that some extra bands...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectinophora gossypiella; Gamma rays; RAPD-PCR; Elements; DNA.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100436
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Alterations in Biomarkers Associated with Sterility in Pectinophora gossypiella (Saunders) Induced by Gamma Irradiation BABT
Ali,Hanaa Mahmoud; Moustafa,Hemat Zakaria; Sayed,Rehab Mahmoud.
ABSTRACT Nowadays, radiation technology is widely used to produce changes in Biosystems. The goal of this work is to determine the variation induced male Pectinophora gossypiella in gamma-irradiated as pupae using 50Gy and 150Gy. Comparing elements composition and DNA (using RAPD-PCR) between substerile 50Gy and the sterile dose 150Gy in P. gossypiella showed variation between them. Potassium (K) was the most abundant elements in unirradiated and irradiated males followed by magnesium (Mg). The percentage of heavy metals as copper, zinc, and cadmium concurrent with K was directly proportional to the radiation dose. While the percentage of Mg, Phosphorous and calcium decreased as the radiation dose increased. The results also revealed that some extra bands...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectinophora gossypiella; Gamma rays; RAPD-PCR; Elements; DNA.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100401
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Alternative cytoplasmic male sterility systems in sorghum and their utilization Open Agri
A.A. ( A. Ashok Kumar ).
Palavras-chave: Cytoplasm; Genes; Sorghum; Hybrids; Heterosis; Transcription; DNA; Genetic structures; Segregation; Genomes.
Ano: 2008 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3149
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An effective method for cloning of partial MADS-box genes related to flower development in groundnut Open Agri
Yuan, M..
Palavras-chave: Genes; PCR; DNA; Cloning; Transcription factors; Genomes; Transcription; Genetic structures; Nucleotides; Molecular cloning.
Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3213
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Analise dos processsos de quebra, sintese e reparo do DNA durante a profase meiotica em Lilium spp. Infoteca-e
ALVES, R. M.; NASCIMENTO FILHO, F. J. do.
Celulas meioticas de lirio, microsporocitos, tem sido amplamente utilizadas para estudos bioquimicos combinados. O sincronismo natural das celulas meioticas, a duracao arelativamente longa da profase (2 a 5 dias) e a habilidade das celulas desenvolverem-se in vitro possibilitou analisar cada intervalo da sintese de DNA, separadamente.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Lillium; Brasil; Sao Paulo; Piracicaba; Species; Plants; Nucleotide sequence; DNA; Espécie; Meiose; Planta.
Ano: 1984 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/670465
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Antimicrobial histones and DNA traps in invertebrate immunity: evidences in Crassostrea gigas. ArchiMer
Poirier, Aurore C.; Schmitt, Paulina; Rosa, Rafael D.; Vanhove, Audrey S.; Kieffer-jaquinod, Sylvie; Rubio, Tristan P.; Charriere, Guillaume M.; Destoumieux-garzon, Delphine.
Although antimicrobial histones have been isolated from multiple metazoan species, their role in host defense has long remained unanswered. We found here that the hemocytes of the oyster Crassostrea gigas release antimicrobial H1-like and H5-like histones in response to tissue damage and infection. These antimicrobial histones were shown to be associated with extracellular DNA networks released by hemocytes, the circulating immune cells of invertebrates, in response to immune challenge. The hemocyte-released DNA was found to surround and entangle vibrios. This defense mechanism is reminiscent of the neutrophil extracellular traps (ETs) recently described in vertebrates. Importantly, oyster ETs were evidenced in vivo in hemocyte-infiltrated interstitial...
Tipo: Text Palavras-chave: Antimicrobial Peptide (AMP); DNA; Innate Immunity; Invertebrate; Reactive Oxygen Species (ROS); NET; Mollusk.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00217/32866/31784.pdf
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Aplicação e evolução dos métodos moleculares para o estudo da biodiversidade do rizóbio. Infoteca-e
STRALIOTTO, R.; RUMJANEK, N. G..
A extensão da biodiversidade microbiana. A diversidade do rizóbio. Aplicações da biologia molecular nos estudos de diversidade e taxonomia do rizóbio. Métodos fenotípicos clássicos. Ánalise numerica de dados fenotípicos. Ánalise de isoenzimas. Pirólise. Métodos sorológicos. SDS_PAGE. Métodos genotípicos. Determinação da relação de bases do DNA (RFLP). Homologia do DNA> Polimorfismo nos fragmentos de restrição do DNA (RFLP). Estudos baseados no DNA ribossomal. A reação de polimeras em cadeia. Técnicas de fingerprinting do DNA baseadas em PCR
Tipo: Documentos (INFOTECA-E) Palavras-chave: Ácido Nucléico; Biologia Molecular; Biodiversidade; DNA; Fenótipo; Genótipo; Isoenzima; Rhizobium; Taxonomia..
Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/624276
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Arachis hoehnei, the probable B genome donor of Arachis hypogaea based on crossability, cytogenetical and molecular studies Open Agri
Mallikarjuna, N..
Palavras-chave: Genomes; Hybrids; Polyploidy; Cytogenetics; DNA; Groundnuts; Mosses; Genetic polymorphism; Polymorphism; Genetic improvement.
Ano: 2006 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3177
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As expansoes do milho - Zea mays, L. - para a América do Sul, baseado no resgate e estudo de DNA anciao de amostras arqueológicas. Infoteca-e
FREITAS, F. de O..
bitstream/CENARGEN/23671/1/bp032.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Amostra arqueologica; America do Sul.; DNA; Genética; Milho; Zea Mays..
Ano: 2002 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/184682
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Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi Genet. Mol. Biol.
Torres-Silva,Carolina Furtado; Repolês,Bruno Marçal; Ornelas,Hugo Oliveira; Macedo,Andréa Mara; Franco,Glória Regina; Junho Pena,Sérgio Danilo; Tahara,Erich Birelli; Machado,Carlos Renato.
Abstract Trypanosoma cruzi is the etiological agent of Chagas disease, a public health challenge due to its morbidity and mortality rates, which affects around 6-7 million people worldwide. Symptoms, response to chemotherapy, and the course of Chagas disease are greatly influenced by T. cruzi‘s intra-specific variability. Thus, DNA mutations in this parasite possibly play a key role in the wide range of clinical manifestations and in drug sensitivity. Indeed, the environmental conditions of oxidative stress faced by T. cruzi during its life cycle can generate genetic mutations. However, the lack of an established experimental design to assess mutation rates in T. cruzi precludes the study of conditions and mechanisms that potentially produce genomic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: T. cruzi; DNA; Mutation frequency; H2O2.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300466
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Autophagy plays an important role in protecting Pacific oysters from OsHV-1 and Vibrio aestuarianus infections ArchiMer
Moreau, Pierrick; Moreau, Kevin; Segarra, Amelie; Tourbiez, Delphine; Travers, Marie-agnes; Rubinsztein, David C.; Renault, Tristan.
Recent mass mortality outbreaks around the world in Pacific oysters, Crassostrea gigas, have seriously affected the aquaculture economy. Although the causes for these mortality outbreaks appear complex, infectious agents are involved. Two pathogens are associated with mass mortality outbreaks, the virus ostreid herpesvirus 1 (OsHV-1) and the bacterium Vibrio aestuarianus. Here we describe the interactions between these 2 pathogens and autophagy, a conserved intracellular pathway playing a key role in innate immunity. We show for the first time that autophagy pathway is present and functional in Pacific oysters and plays an important role to protect animals from infections. This study contributes to better understand the innate immune system of Pacific...
Tipo: Text Palavras-chave: Autophagy; Crassostrea gigas; OsHV-1; Pacific oyster; Vibrio aestuarianus; ATG; Autophagy-related; Atg8-PE; Atg8-phosphatidylethenolamine; DNA; Deoxyribonucleic acid; Hpi; Hours postinfection; LC3-II; Cleaved; Lipidated and autophagosome-associated form of LC3; MAP1LC3A; B (LC3A; B); Microtubule-associated proteins 1 light chain 3 alpha.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00269/37989/36072.pdf
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