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18S-rDNA SEQUENCING, ENZYME PATTERNS AND MORPHOLOGICAL CHARACTERIZATION OF TRICHOPHYTON ISOLATES BJM
Nascimento,Adriana Mendes do; Martinez-Rossi,Nilce M..
Dermatophytes, capable to use keratin of the host for nutrition, belong to one of the major groups of pathogenic fungi. Since dermatophytes are a closely related group they share various common features, and the morphology of isolates of a given species can be atypical, making species identification and differentiation even more difficult. Many methods have been explored in attempts to distinguish dermatophytes, but the combined use of different approaches for the investigation of the intraspecific and interspecific variability of Trichophyton continues to be scarce. Some studies have shown that amplified fragments of the small ribosomal DNA subunit 18S contains variable regions which can be used to discriminate between medically relevant yeast species,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichophyton; 18S-rDNA sequencing; Extracellular enzymes; Dermatophyte.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300004
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Dermatophytes and saprobe fungi isolated from dogs and cats in the city of Fortaleza, Brazil Arq. Bras. Med. Vet. Zootec.
Paixão,G.C.; Sidrim,J.J.C.; Campos,G.M.M.; Brilhante,R.S.N.; Rocha,M.F.G..
The possible involvement of saprobe fungi in dermatomycoses, as well as the determination of the incidence of dermatophytes in dogs and cats were studied. During a period of one year, 74 dogs and 18 cats, with cutaneous lesions suggesting mycoses were included in this study. The mycological analyses were conducted by direct microscopy and by fungal culture on Sabouraud agar, chloramphenicol Sabouraud agar and mycosel agar. Of the 92 samples, 21 resulted in positive cultures for dermatophytes. Dermatophyte fungi pure cultures were obtained from 13 samples. A simultaneous growth of dermatophytes plus saprobe fungi was observed in 8 of the samples. Of the remaining 71 samples, no fungal growth was observed in 10 samples, and at minimum the growth of one...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Cat; Dermatophyte; Saprobe fungi; Mycosis.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000500010
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Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum Genet. Mol. Biol.
Cervelatti,Eliane P.; Ferreira-Nozawa,Monica S.; Aquino-Ferreira,Roseli; Fachin,Ana Lúcia; Martinez-Rossi,Nilce M..
The electrophoretic karyotype of the dermatophyte Trichophyton rubrum was established using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Five chromosomal bands of approximately 3.0 to 5.8 megabase pairs (Mbp) each were observed and together indicated that 22.05 Mbp of the total genome are organized as chromosomal macromolecules. In addition to establishing the number and size of T. rubrum chromosomes, these results open perspectives for the construction of chromosome-specific libraries and for the physical mapping of genes of interest, thus permitting future gene linkage studies in this pathogen. A detailed understanding of the karyotype and genomic organization of T. rubrum should contribute to further genetic, taxonomic and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichophyton rubrum; Fungal chromosomes; Electrophoretic karyotype; Dermatophyte; Contour-clamped homogeneous electric field.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100016
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In Vitro additive effect on griseofulvin and terbinafine combinations against multidrug-resistant dermatophytes BJPS
Lana,Aline Jacobi Dalla; Pippi,Bruna; Carvalho,Anderson Ramos; Moraes,Renata Cougo; Kaiser,Samuel; Ortega,George Gonzalez; Fuentefria,Alexandre Meneghello; Silveira,Gustavo Pozza.
ABSTRACT Griseofulvin (GF) and terbinafine (TF) are commonly used drugs to treat dermatophytosis, a fungal infection of the skin. Today there is an increase in drug resistance to these antifungals which highlight the need for alternative synergistic therapies. Minimum Inhibitory Concentration (MIC) of GF and TF were determined against fungi clinical isolates from local hospitals with values ranging 0.03-2.0 µg mL-1 and 0.24-4.0 µg mL-1, respectively. A checkboard test was used to determine the combination of GF:TF which could induce an additive effect against the fungi isolates Multidrug-resistant isolates showed susceptibility after treatment with 16:2 µg mL-1 GF:TF. An MTT assay further verified that GF and TF combinations have greater additive effect...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Griseofulvin; Terbinafine; Dermatophyte; Multidrug-resistant fungi; Drug combination..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200623
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