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Registros recuperados: 69
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16s-23S rDNA: polymorphisms and their use for detection and identification of Xylella fastidiosa strains BJM
Martinati,Juliana Camargo; Pacheco,Flávia Tereza Hansen; Miranda,Vitor Fernandes Oliveira de; Tsai,Siu Mui.
Strains of Xylella fastidiosa from several hosts (coffee, citrus, grape, almond, oleander, peach, plum, etc.) were characterized by analyzing the content of the nucleotide sequences of 16S-23S rDNA (coding for a small subunit ribosomal RNA) spacer region (ITS). Current methods for sequencing the ITS region yields partial sequences that do not contribute with significant information. According to this fact, new primers have been designed in order to obtain a complete sequence and facilitate the sequencing. The complete 16S-23S sequences from 08 strains were amplified through PCR using the primers designed in our lab. The 16S-23S sequences obtained were compared with 52 others sequences entries in GenBank database. The results revealed a higher level of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; 16S-23S rDNA; Intergenic spacer; Identification; Detection.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100033
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A long term study of bonamiosis in Quiberon bay, France ArchiMer
Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Berthe, Franck.
Bonamiosis was first reported in association with mass mortality of flat oysters, Ostrea edulis, in June 1979 in L'Ile Tudy, Brittany. The disease rapidly spread to all of the oyster farming areas in France but also in other European countries. The French flat oyster production, which already suffered from another protozoan disease, marteiliosis, decreased from 5500 mt in 1979 to less than 2000 mt after 1980. In 2001, about 350 French farms sell 1650 mt of flat oysters. This production mainly relies on natural spat collection which specially occurs in Quiberon bay. One third of this spat is transferred from South to North Brittany for further growth. Quiberon bay constitutes an interesting site regarding the surveillance of bonamiosis because of the...
Tipo: Text Palavras-chave: Detection; Bonamia ostreae; Protozoan; Diseases; Pathology; Ostrea edulis; Oysters.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3314.pdf
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An intercomparison study of the determination of sulfite in tropical shrimps by the West European Fish Technologists' Association (WEFTA) OMA
Luten, J.; Bouquet, W.; Oehlenschläger, J.; Meetschen, U.; Etienne, M.; Stroud, G.; Bykowski, P.; Batista, I.; Vyncke, W.; Stefansson, G..
Eight WEFTA laboratories participated in two series of intercomparison exercises on sulfite determination in tropical shrimps. Samples of tropical shrimps were spiked with sodium metabisulfite (Na<sub>2</sub>S<sub>2</sub>O<sub>5</sub>) and hydroxymethylsulfonate (HMS) at a level of 25-90 mg SO<sub>2</sub>/kg. Most of the laboratories determined the sulfite content with (modified) methods of Monier-Williams or De Vries et al. The overall mean recovery of sulfite was rather low (47-60%), which may be attributed to an irreversible reaction of sulfite with the tropical shrimps. The repeatability of the methods within the participating laboratories was good. However, reproducibility among the laboratories was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Detection; Fishery products; Food technology; International cooperation; Quality control; Recovery; Shrimp fisheries; Sulphites.
Ano: 1997 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=5742
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Analytical Methods for Virus Detection in Water and Food ArchiMer
Bosch, Albert; Sanchez, Gloria; Abbaszadegan, Morteza; Carducci, Annalaura; Guix, Susana; Le Guyader, Soizick; Netshikweta, Rembuluwani; Pinto, Rosa M.; Van Der Poel, Wim H. M.; Rutjes, Saskia; Sano, Daisuke; Taylor, Maureen B.; Van Zyl, Walda B.; Rodriguez-lazaro, David; Kovac, Katarina; Sellwood, Jane.
Potential ways to address the issues that relate to the techniques for analyzing food and environmental samples for the presence of enteric viruses are discussed. It is not the authors' remit to produce or recommend standard or reference methods but to address specific issues in the analytical procedures. Foods of primary importance are bivalve molluscs, particularly, oysters, clams, and mussels; salad crops such as lettuce, green onions and other greens; and soft fruits such as raspberries and strawberries. All types of water, not only drinking water but also recreational water (fresh, marine, and swimming pool), river water (irrigation water), raw and treated sewage are potential vehicles for virus transmission. Well over 100 different enteric viruses...
Tipo: Text Palavras-chave: Enteric viruses; Gastroenteritis; Hepatitis; Detection; Concentration.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00031/14191/11465.pdf
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Application du laser a la detection sous-marine: Experimentation d'un imageur ArchiMer
Kervern, G; Legall, A.
Nowadays, side-looking sonar is the only equipment able to carry out long-range underwater imaging. The use of optics is limited to short-range identification by means of conventional video-cameras. However, active optical systems (lidars) manufacturing, using a blue-green laser, will enable optics to hold its place in the field of long-range underwater imaging. After a short comparison between acoustics and optics in underwater detection and a description of the proper features of underwater optical propagation, we present the different conceivable imaging principles and propose a method to determine their performances. The experimentations described concern a lidar imager mock-up using one of the imaging principles proposed. The pictures obtained deal...
Tipo: Text Palavras-chave: Optics; Lasers; Detection; Acoustics; Underwater cameras; Sonar imagery; Imaging techniques; Underwater photography; Marine technology; Side scan sonar.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/acte-1139.pdf
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Assessment of biochemical methods to detect enzymatic depolymerization of polysaccharides ArchiMer
Rigouin, Coraline; Delbarre Ladrat, Christine; Sinquin, Corinne; Colliec-jouault, Sylvia; Dion, M.
To find biocatalyst allowing depolymerization of new polysaccharides, one needs to get a sensitive and well adapted method to a screening program. This led us to compare biochemical methods of detection of the depolymerization. Currently used methods such as reducing sugars assays, double bond monitoring or molecular weight determination were tested to follow the kinetic of depolymerization with different enzyme / polysaccharide couples. The range of concentrations of assorted enzymes allowed us to identify the most sensitive and appropriate method to detect polysaccharide degradation. Reducing sugars assays are quantitative, sensitive and almost usable with all kind of polysaccharide but some compounds may interfere with them. When polysaccharide is...
Tipo: Text Palavras-chave: Detection; Enzyme; Depolymerization; Carbohydrate.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2008/publication-4723.pdf
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Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment BJM
Oliveira,I.C.; Almeida,R.C.C.; Hofer,E.; Almeida,P.F..
A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 10(8) pfu/mL) without affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration. Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously tested. The protocol was faster...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria; Bacteriophage A511; Laser light; Detection; Skimmed milk.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300040
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Capture immunoassay for LT detection produced by enterotoxigenic Escherichia coli in bacterial isolates BJM
Menezes,Caroline Anunciação; Gonçalves,Danielle Silva; Amianti,Jackeline; Fernandes,Irene; Taddei,Carla Romano; Koga,Paula Célia Mariko; Trabulsi,Luiz Rachid; Martinez,Marina Baquerizo; Piazza,Roxane Maria Fontes.
A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has beendeveloped. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture immunoassayis a sensitive tool for detection of E. coli, which produces heat-labile enterotoxin, and is suitable for use in clinical laboratories and epidemiological surveys in developing world.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Detection; Polyclonal antibody; Monoclonal antibody; Thermo-labile toxin; E. coli.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500004
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Comparação de testes sorológicos e molecular para diagnóstico da Artrite Encefalite Caprina e avaliação clínica da glândula mamária de caprinos leiteiros infectados. Repositório Alice
AZEVEDO, D. A. A. de; PINHEIRO, R. R.; SANTOS, V. W. S. dos; DAMASCENO, E. M.; SOUSA, A. L. M. de; ARAÚJO, J. F.; ANDRIOLI, A.; SIDER, L. H.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S..
Background: Caprine Arthritis Encephalitis (CAE) is a disease that causes productive losses in dairy goat flocks due to the reduction in milk production, followed by lesions in joints and mammary glands. An early diagnosis is essential, considering that there is frequent occurrence of asymptomatic animals. Hence, this study aimed to perform a comparison of immunological and molecular based diagnostic tests, represented by Agar Gel Immunodiffusion (AGID), Western Blot (WB) and nested Polymerase Chain Reaction (nPCR). In addition, the mammary glands (MG) of dairy goats were clinically evaluated. Materials, Methods & Results: Blood collection and clinical examination were performed in 1191 dairy goats of 12 farms located in Northeastern and Southeastern...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Caprino leiteiro; Detecção; Exame clínico; Lentivírus caprino; CAE; Caprino; Cabra Leiteira; Doença Animal; Vírus; Goat diseases; Dairy goats; Clinical examination; Lentivirus; Detection; Caprine arthritis encephalitis virus.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112509
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Deteccao de virus em sementes. Infoteca-e
ALMEIDA, A. M. R..
bitstream/item/164456/1/deteccao-de-virus-em-sementes.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Semente; Virus; Deteccao; Quarentena; Seed; Detection; Quarantine.
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/460704
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Detecção de vírus por RT-PCR, hibridização "DOT-BLOT" e DOT-ELISA em milho com mosaico comum. Repositório Alice
ALMEIDA, A.C.L.; OLIVEIRA, E.; RESENDE, R..
O mosaico comum do milho e causado por um complexo viral constituido por quatro potyvirus distintos e pode reduzir significativamente a producao do milho (Zea mays). Frequentemente, a diagnose dessa virose e dificultada devido a recuperacao dos sintomas pelas plantas ou a ocorrencia de infeccoes simultaneas com outros virus e/ou com fitoplasmas e espiroplasmas. O objetivo deste trabalho foi desenvolver metodos eficazes para a deteccao do complexo viral utilizando-se RT-PCR com "primers" degenerados, hibridizacao "dot-blot" e dot-ELISA. O complexo viral foi multiplicado com plantas de milho por meio de inoculacao mecanica e posteriormente purificado. A preparacao purificada foi utilizada para a producao de antisoro policlonal e as IgGs purificadas foram...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Milho; Potyvirus; Diagnostico; Mosaico comum; Maize; Detection; Virus; Diagnostic.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484401
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Detection by Rt-Pcr and Molecular Characterization of Tremovirus A Obtained from Clinical Cases of Avian Encephalomyelitis (AE) Outbreaks in Brazil Rev. Bras. Ciênc. Avic.
Torre,D De la; Nuñez,LF; Parra,SHS; Astolfi-Ferreira,CS; Ferreira,AJP.
ABSTRACT This study determined the presence of Tremovirus A as the possible agent related to Avian Encephalomyelitis in broiler chicks from the states of São Paulo (SP) Paraná (PR), Goiás (GO), Santa Catarina (SC) and Rio Grande do Sul (RS), between the years 2006 and 2015. Samples of the nervous, digestive, respiratory, immune, and renal systems, plus muscular organs from broiler chicks with neurological problems such as ataxia and muscle tremors, and four (4) commercial vaccines as positive control, were tested by reverse-transcriptase (RT-PCR) amplification and DNA sequencing. A highly conserved region (P1) of the viral genome, was used to amplify a segment which encodes a structural protein VP4. Out of 112 samples, 46 were positive (42%) for Tremovirus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken; Tremovirus A; Avian Encephalomyelitis; Detection; Molecular characterization.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300527
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Detection de fissure sur structure en mer en presence de salissure marine projet "Crabe" ArchiMer
Daste, S.
The "CRABE" method is a crack detection technique based on the use of low electromagnetic frequencies. This (patented) method relies on a global injection of current within the structure under inspection. The presence of a crack shall cause a disruption in the current flow, and consequently of the magnetic field off the surface in the vicinity of a defect. It is this magnetic signature which is detected by the magnetic sensor used with "CRABE". The major advantage of this process, developed by TRAVOCEAN and the LETI Division of CEA with the help of IFREMER, appears clearly: No preliminary cleaning of the welds to be inspected is required, since no contact is necessary. Similarly, it shall be possible to inspect the whole of structures to a total cost all...
Tipo: Text Palavras-chave: Offshore structures; Fouling; Underwater exploration; Steel structures; Sensors; Cracks; Detection; Unmanned vehicles; Marine technology.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/acte-1161.pdf
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Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review ArchiMer
Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan.
Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1...
Tipo: Text Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf
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Detection of ostreid herpesvirus 1 (OsHV-1) DNA in seawater by PCR: influence of water parameters in bioassays ArchiMer
Vigneron, Vassilia; Solliec, Gaelle; Montanie, Hélène; Renault, Tristan.
Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses; in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus I (OsHV-1) detection by polymerase chain reaction (PCR). Viral DNA extracted from purified particles or virions present in infected oyster larvae were detected by PCR after storage in different media at different temperatures. The lowest detection threshold was found using distilled water or Tris EDTA buffer. In seawater, the threshold was higher. The use of sterile media permitted...
Tipo: Text Palavras-chave: Temperature; PCR; Detection; Seawater; Viral DNA; Oyster herpesvirus 1.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-2909.pdf
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Detection of ostreid herpesvirus-1 (OsHV-1) by PCR using a rapid and simple method of DNA extraction from oyster larvae ArchiMer
Batista, Frederico; Taris, Nicolas; Boudry, Pierre; Renault, Tristan.
A DNA extraction procedure was developed for the detection of ostreid herpesvirus-1 (OsHV-1) using the polymerase chain reaction (PCR) in oyster larvae. The DNA extraction procedure developed was tested on 8 larval samples. Abnormal nuclei with characteristic features associated with OsHV-1 infections were only observed in samples in which the viral DNA was detected by PCR. A previously described competitive PCR method was applied to detect inhibition during PCR reactions. The results show that the method can be used on small amounts of oyster larvae (3 mg) for the detection of OsHV-1 DNA by PCR.
Tipo: Text Palavras-chave: DNA extraction; Detection; Larvae; Oyster; OsHV 1; Herpesvirus.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-2916.pdf
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Detection of Salmonella sp. from porcine origin: a comparison between a PCR method and standard microbiological techniques BJM
Castagna,Sandra Maria Ferraz; Muller,Monika; Macagnan,Marisa; Rodenbusch,Carla Rosane; Canal,Cláudio Wageck; Cardoso,Marisa.
The aim of this study was to compare a polymerase chain reaction (PCR) method combined with selective enrichment in Rappaport-Vassiliadis broth (PCR-RVB) with standard microbiological techniques (SMT) for the generic detection of Salmonella in samples of porcine origin. Two hundred sixty eight field samples consisting of 42 sets of pooled porcine mandibular lymph nodes and tonsils, 44 samples of intestinal content, 38 pork sausage meat samples and 144 samples of feed collected from swine farms were submitted to the PCR-RVB and SMT protocols. Salmonella was detected in 54 samples using the PCR-RVB assay and in 42 samples by SMT, three of the SMT Salmonella-positive samples (one each of S. Derby, S. Panama and S. Typhimurium) being Salmonella-negative by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Detection; PCR; Pork; Salmonella; Swine.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400013
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Detection of Salmonella spp, Salmonella Enteritidis and Typhimurium in naturally infected broiler chickens by a multiplex PCR-based assay BJM
Paião,F.G.; Arisitides,L.G.A.; Murate,L.S.; Vilas-Bôas,G.T.; Vilas-Boas,L.A.; Shimokomaki,M..
The presence of Salmonella in the intestinal tract, on the chickens skin and among their feathers, may cause carcasses contamination during slaughtering and processing and possibly it is responsible by the introduction of this microorganism in the slaughterhouses. A rapid method to identify and monitor Salmonella and their sorovars in farm is becoming necessary. A pre-enriched multiplex polymerase chain reaction (m-PCR) assay employing specific primers was developed and used to detect Salmonella at the genus level and to identify the Salmonella enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterica serovar Typhimurium (S. Typhimurium) in broiler chicken swab samples. The method was validated by testing DNA extract from 90 fresh culture...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aviculture; Bacteria; Detection.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100005
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Detection of Xanthomonas oryzae pv. oryzae by NCM-ELLISA in naturally infected rice plants International Rice Research Institute
Suryadi, Y.; Kadir, T. S..
p.34-35
Palavras-chave: Xanthomonas oryzae pv. oryzae; Plant pathogenic bacteria; Detection; ELISA.
Ano: 2004 URL: http://hdl.handle.net/123456789/986
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Development of a new diagnostic tool (Mini-Array) for ostreid Herpesvrus 1 (OsHV-1) detection in the Pacific oyster Crassostre gigas ArchiMer
Varsamos, S.; Pepin, Jean-francois; Sauvage, Christopher; Renault, Tristan.
OsHV-1 was the first herpesvirus to be identified in an invertebrate host and is associated to mortalities in the Pacific oyster (Crassostrea gigas) and other economically important bivalve species. Infections due to OsHV-1 are frequently reported and cause high mortality rates in larvae and spat of C. gigas. Detection of this pathogen is usually performed by PCR and/or in situ hybridization. This presentation will focus on the development of a new molecular diagnostic tool for OsHV-1 (and OsHV-1 variant 1) detection: the mini-array. This detection system, which allows multiple pathogens detection, combines viral DNA amplification by PCR and a specific hybridisation on a nylon membrane, revealed by a colorimetric reaction (DNA chip). Multiplex PCR involves...
Tipo: Text Palavras-chave: Diagnostic; Crassotrea gigas; Oyster; Detection; Herpesviridae; OsHV 1.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3652.pdf
Registros recuperados: 69
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