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Registros recuperados: 22 | |
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Dubousquet, Vaimiti; Gros, Emmanuelle; Berteaux-lecellier, Veronique; Viguier, Bruno; Raharivelomanana, Phila; Bertrand, Cédric; Lecellier, Gael J.. |
Temperature can modify membrane fluidity and thus affects cellular functions and physiological activities. This study examines lipid remodelling in the marine symbiotic organism, Tridacna maxima, during a time series of induced thermal stress, with an emphasis on the morphology of their symbiont Symbiodinium. First, we show that the French Polynesian giant clams harbour an important proportion of saturated fatty acids (SFA), which reflects their tropical location. Second, in contrast to most marine organisms, the total lipid content in giant clams remained constant under stress, though some changes in their composition were shown. Third, the stress-induced changes in fatty acid (FA) diversity were accompanied by an upregulation of genes involved in lipids... |
Tipo: Text |
Palavras-chave: Thermal stress; Fatty acids; Differential expression; Antioxidant; Symbiodinium; Tridacna maxima. |
Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00361/47195/79514.pdf |
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OLIVEIRA, F. A. de; VIGNA, B. B. Z.; SILVA, C. C. da; FAVERO, A. P.; MATTA, F. de P.; AZEVEDO, A. L. S.; SOUZA, A. P. de. |
RNA sequencing (RNA-seq) is the most effective method for simultaneously predicting new transcripts and identifying differentially expressed genes among distinct tissues, genotypes, abiotic conditions and developmental stages [1]. Conversely, considering the large amount of data generated from RNA-seq, new approaches that efficiently extract meaningful associations from highly multivariate datasets are needed [2]. Transcriptome coexpression studies can show how complex phenotypes depend on the activity of coordinated batteries of genes [3]. Therefore, the construction of coexpression networks based on gene expression data using correlation metrics provides valuable information regarding alterations in biological systems in response to differential gene... |
Tipo: Artigo de periódico |
Palavras-chave: Differential expression; Gene coexpression network; RNA sequencing; Apomixis; Paspalum. |
Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121557 |
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VILLETH, G. R. C.; CARMO, L. S. T.; SILVA, L. P.; FONTES, W.; GRYNBERG, P.; SARAIVA, M.; BRASILEIRO, A. C. M.; CARNEIRO, R. M. D.; OLIVEIRA, J. T. A.; GROSSI-DE-SA, M. F.; MEHTA, A.. |
Tipo: Separatas |
Palavras-chave: 2-DE; Differential expression; MS; Nematode–plant interaction; Plant proteomics.. |
Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1023130 |
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OLIVEIRA, K. C. de; GUIMARÃES, P. de S.; BAZIOLI, J. M.; MARTINATI, J. C.; SANTOS, M. M. dos; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P.. |
Cloning of superior coffee plants by somatic embryogenesis can assist breeding programs on reducing the cost and time for launch of new cultivars. This study aimed to evaluate the efficiency of this methodology for cloning coffee trees with high heterozygosity, and to gather evidence that clonal progenies are faithful copies of mother plants. Selected plants IAC1 and IAC 2 from Coffea arabica breeding populations, resistant to leaf rust and leaf miner, respectively, were cloned via indirect somatic embryogenesis. Expression of selected genes involved in biological processes potentially affected by in vitro cultivation was evaluated by quantitative analysis. Genes encoding proteins associated with maintenance of DNA integrity and control of cell cycle... |
Tipo: Artigo de periódico |
Palavras-chave: In vitro cultivation; Differential expression; Coffea Arábica; Somaclonal variation. |
Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113261 |
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Teaniniuraitemoana, Vaihiti; Huvet, Arnaud; Levy, Peva; Klopp, Christophe; Lhuillier, Emeline; Gaertner-mazouni, Nabila; Gueguen, Yannick; Le Moullac, Gilles. |
Background Black pearl farming is based on culture of the blacklip pearl oyster Pinctada margaritifera (Mollusca, lophotrochozoa), a protandrous hermaphrodite species. At first maturation, all individuals are males. The female sex appears progressively from two years old, which represents a limitation for broodstock conditioning for aquaculture production. In marine mollusks displaying hermaphroditic features, data on sexual determinism and differentiation, including the molecular sex determining cascade, are scarce. To increase genomic resources and identify the molecular mechanisms whereby gene expression may act in the sexual dimorphism of P. margaritifera, we performed gonad transcriptome analysis. Results The gonad transcriptome of P. margaritifera... |
Tipo: Text |
Palavras-chave: Pinctada margaritifera; Gametogenesis; Transcriptome; Differential expression; Sex determinism. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00201/31194/29593.pdf |
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Wang,Z; Deng,X; Wang,AI; Liu,R. |
ABSTRACTHMOX1 is an important gene in biosynthesis of the eggshell pigment of blue eggs. Previous studies found that HMOX1 is highly expressed in the shell gland of hens laying blue eggs (BlueH) compared with hens laying brown eggs (BrownH); however, the reasons for the differential expression are unclear. In this study five single nucleotide polymorphism (SNP) in HMOX1 were genotyped in 111 BlueH and 115 BrownH. The association of haplotypes of these SNP with the blue egg phenotype was tested. Haplotype-specific expression of HMOX1 was detected in the shell gland. The interaction of sequence variants and transcription factors was analyzed using electrophoretic mobility shift assay (EMSA). A TG haplotype covering upstream 1.4 kb region of HMOX1 was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken; Blue egg; Biliverdin; HMOX1; Differential expression. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000300267 |
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Mehta,Angela; Rosato,Yoko B.. |
Xanthomonas axonopodis pv. citri is a phytopathogenic bacterium responsible for citrus canker, a serious disease which causes severe losses in citriculture around the world. In this study we report the differential expression of X. axonopodis pv. citri in response to specific treatments by using Representational Difference Analysis of cDNA (cDNA RDA). cDNAs from X. axonopodis pv. citri cultured in the presence of leaf extract of the host plant (Citrus sinensis), in vivo, as well as in the complex medium were hybridized against cDNA of the bacterium grown in the minimal medium. Sequencing of the difference products obtained after the second and third hybridizations revealed a total of 37 distinct genes identified by homology searches in the genome of X.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus canker; Differential expression; Leaf extract; In vivo. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100024 |
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Lemer, Sarah; Saulnier, Denis; Gueguen, Yannick; Planes, Serge. |
Background: Color polymorphism in the nacre of pteriomorphian bivalves is of great interest for the pearl culture industry. The nacreous layer of the Polynesian black-lipped pearl oyster Pinctada margaritifera exhibits a large array of color variation among individuals including reflections of blue, green, yellow and pink in all possible gradients. Although the heritability of nacre color variation patterns has been demonstrated by experimental crossing, little is known about the genes involved in these patterns. In this study, we identify a set of genes differentially expressed among extreme color phenotypes of P. margaritifera using a suppressive and subtractive hybridization (SSH) method comparing black phenotypes with full and half albino individuals.... |
Tipo: Text |
Palavras-chave: Differential expression; Biomineralization; Nacre; Pearl; Pigmentation; Albino. |
Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00275/38627/37167.pdf |
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TIZIOTO, P.; GASPARINI, G.; SOUZA, M. M.; MUDADU, M. de A.; COUTINHO, L. L.; MOURÃO, G. B.; THOLON, P.; MEIRELLES, S. L. C.; TULLIO, R. R.; ROSA, A. do N.; ALENCAR, M. M. de; MEDEIROS, S. R.; SIQUEIRA, F.; FEIJO, G. L. D.; NASSU, R. T.; REGITANO, L. C. de A.. |
The potassium inwardly rectifying channel, subfamily J, member 11 (KCNJ11) gene was investigated as a candidate for meat tenderness based on the effects reported on muscle for KCNJ11 gene knockout in rat models and its position in a quantitative trait locus (QTL) for meat tenderness in the bovine genome. Sequence variations in the KCNJ11 gene were described by sequencing six amplified fragments, covering almost the entire gene. We identified single nucleotide polymorphisms (SNP) and validated them by different approaches, taking advantage of simultaneous projects that are being developed with the same Nelore population. By sequencing the KCNJ11 in Nelore steers representing extreme phenotypes for Warner-Bratzler shear force (WBSF), it was possible to... |
Tipo: Artigo de periódico |
Palavras-chave: Candidate gene; Bovine; Differential expression; Molecular markers; Warner Bratzler; Shear force; Beef; Meat. |
Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/982035 |
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ARRAES, F. B. M.; VASQUEZ, D. D. N.; TAHIR, M.; PINHEIRO, D. H.; FAHEEM, M.; FREITAS-ALVES, N. S.; MOREIRA-PINTO, C. E.; MOREIRA, V. J. V.; PAES-DE-MELO, B.; LISEI-DE-SA, M. E.; MORGANTE, C. V.; MOTA, A. P. Z.; LOURENÇO-TESSUTTI, I. T.; TOGAWA, R. C.; GRYNBERG, P.; FRAGOSO, R. da R.; ALMEIDA-ENGLER, J. de; LARSEN, M. R.; GROSSI-DE-SA, M. F.. |
The root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) and highly tolerant (PI595099) Glycine max genotypes 4, 12, and 30 days after RKN infestation. After in silico analysis, we described major defense molecules and mechanisms considered constitutive responses to nematodeinfestation, such as mTOR, PI3K-Akt, relaxin, and thermogenesis. The integrated data allowed us to identify protein families and metabolic pathways exclusively regulated in tolerant soybean... |
Tipo: Artigo de periódico |
Palavras-chave: Root-knot nematode; Differential expression; Meloidogyne Incognita; Soja; Glycine Max; Transcriptome; Proteome; Phenylpropanoids. |
Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148619 |
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Engels,Cibelle; Fuganti-Pagliarini,Renata; Marin,Silvana Regina Rockenbach; Marcelino-Guimarães,Francismar Corrêa; Oliveira,Maria Cristina Neves; Kanamori,Norihito; Mizoi,Junya; Nakashima,Kazuo; Yamaguchi-Shinozaki,Kazuko; Nepomuceno,Alexandre Lima. |
The loss of soybean yield to Brazilian producers because of a water deficit in the 2011-2012 season was 12.9%. To reduce such losses, molecular biology techniques, including plant transformation, can be used to insert genes of interest into conventional soybean cultivars to produce lines that are more tolerant to drought. The abscisic acid (ABA)-independent Dehydration Responsive Element Binding (DREB) gene family has been used to obtain plants with increased tolerance to abiotic stresses. In the present study, the rd29A:AtDREB2A CA gene from Arabidopsis thaliana was inserted into soybean using biolistics. Seventy-eight genetically modified (GM) soybean lines containing 2-17 copies of the AtDREB2A CA gene were produced. Two GM soybean lines (P1397 and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arabidopsis; Differential expression; Genetically modified organism; Water deficit. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400015 |
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Registros recuperados: 22 | |
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