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Registros recuperados: 11
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An integrated analysis of mRNA and sRNA transcriptional profiles in Coffea arabica L. roots: insights on nitrogen starvation responses. Repositório Alice
SANTOS, T. B. dos; SOARES, J. D. M.; LIMA, J. E.; SILVA, J. C.; IVAMOTO, S. T.; BABA, V. Y.; SOUZA, S. G. H.; LORENZETTI, A. P. R.; PASCHOAL, A. R.; MEDA, A. R.; NISHIYAMA JÚNIOR, M. Y.; OLIVEIRA, U. C. de; MOKOCHINSKI, J. B.; GUYOT, R.; JUNQUEIRA-DE-AZEVEDO, I. L. M.; FIGUEIR, A. V. O.; MAZZAFERA, P.; R. JÚNIO, O.; VIEIRA, L. G. E.; PEREIRA, L. F. P.; DOMINGUES, D. S..
Coffea arabica L. is an important agricultural commodity, accounting for 60% of traded coffee worldwide. Nitrogen (N) is a macronutrient that is usually limiting to plant yield; however, molecular mechanisms of plant acclimation to N limitation remain largely unknown in tropical woody crops. In this study, we investigated the transcriptome of coffee roots under N starvation, analyzing poly-A+ libraries and small RNAs. We also evaluated the concentration of selected amino acids and N-source preferences in roots. Ammonium was preferentially taken up over nitrate, and asparagine and glutamate were the most abundant amino acids observed in coffee roots.We obtained 34,654 assembled contigs by mRNA sequencing, and validated the transcriptional profile of 12...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Nitrogen transport; Differential gene expression; RNA-seq; MicroRNA.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108713
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Differential morphological, physiological, and molecular responses to water deficit stress in sugarcane. Repositório Alice
PEDROZO, C. A.; JIFON, J.; BARBOSA, M. H. P.; SILVA, J. A. da; PARK, J.; GRACIA, N. S..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Differential gene expression; Water deficit stress; Saccharum spp.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1025986
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Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum. Repositório Alice
CORDEIRO, I. B.; CASTRO, D. P.; NOGUEIRA, P. P. O.; ANGELO, P. C. S.; NOGUEIRA, P. A.; GONÇALVES, J. F. C.; PEREIRA, A. M. R. F.; GARCIA, J. S.; SOUZA, G. H. M. F.; ARRUDA, M. A. Z.; EBERLIN, M. N.; ASTOLFI-FILHO, S.; ANDRADE, E. V.; LÓPEZ-LOZANO, J. L..
Chromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis and mass spectrometry.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: CIRCE; Chaperones; Heat shock response; Differential gene expression.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/974472
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Genome-wide gene expression patterns in dikaryon of the basidiomycete fungus Pleurotus ostreatus BJM
Liu,Tianxiang; Li,Huiru; Ding,Yatong; Qi,Yuancheng; Gao,Yuqian; Song,Andong; Shen,Jinwen; Qiu,Liyou.
Abstract Dikarya is a subkingdom of fungi that includes Ascomycota and Basidiomycota. The gene expression patterns of dikaryon are poorly understood. In this study, we bred a dikaryon DK13 × 3 by mating monokaryons MK13 and MK3, which were from the basidiospores of Pleurotus ostreatus TD300. Using RNA-Seq, we obtained the transcriptomes of the three strains. We found that the total transcript numbers in the transcriptomes of the three strains were all more than ten thousand, and the expression profile in DK13 × 3 was more similar to MK13 than MK3. However, the genes involved in macromolecule utilization, cellular material synthesis, stress-resistance and signal transduction were much more up-regulated in the dikaryon than its constituent monokaryons. All...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Differential gene expression; Monoallelic expression; Monokaryon; RNA editing; RNA-Seq.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200380
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Hunting for differentially expressed genes BJMBR
Vedoy,C.G.; Bengtson,M.H.; Sogayar,M.C..
Differentially expressed genes are usually identified by comparing steady-state mRNA concentrations. Several methods have been used for this purpose, including differential hybridization, cDNA subtraction, differential display and, more recently, DNA chips. Subtractive hybridization has significantly improved after the polymerase chain reaction was incorporated into the original method and many new protocols have been established. Recently, the availability of the well-known coding sequences for some organisms has greatly facilitated gene expression analysis using high-density microarrays. Here, we describe some of these modifications and discuss the benefits and drawbacks of the various methods corresponding to the main advances in this field.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Differential gene expression; Differential hybridization; Differential display/DDRT; Subtraction hybridization; Suppressive PCR; DNA microarrays/chips.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700012
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IDENTIFICATION AND QUANTIFICATION OF DIFFERENTIALLY EXPRESSED GENES ASSOCIATED WITH CITRUS BLIGHT (Citrus spp.) Ciência e Agrotecnologia
Abreu,José Renato de; Paiva,Luciano Vilela; Rodríguez,Miguel Angel Dita; Silva,Anderson Tadeu; Henriques,Ariadne Ribeiro; Chalfun-Junior,Antonio.
Brazil is the largest citrus producer in the world, being responsible for more than 20% of its production, which is, however still low due to phytosanitary issues such as citrus blight. Citrus blight is an anomaly whose causes still have not yet been determined, therefore there are no efficient control measures to minimize the production losses with the use of resistant varieties being considered the most appropriate method. However, little is known about the genes involved in the defense response of the plants to this anomaly. Considering that many physiological alterations associated with plant stress responses are controlled at a transcriptional level, in this study we sought the identification and characterization of the gene expression products...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Differential gene expression; Subtractive hybridization; RT-qPCR.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000100032
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Identification of candidate genes for drought tolerance in coffee by high-throughput sequencing in the shoot apex of different Coffea arabica cultivars. Repositório Alice
MOFATTO, L. S.; CARNEIRO, F. de A.; VIEIRA, N. G.; DUARTE, K. E.; VIDAL, R. O.; ALEKCEVETCH, J. C.; COTTA, M. G.; VERDEIL, J-L.; LAPEYRE-MONTES, F.; LARTAUD, M.; LEROY, T.; DE BELLIS, F.; POT, D; RODRIGUES, G. C.; ALSARELLA CARAZZOLLE, M.; PEREIRA, G. A. G.; ANDRADE, A. C.; MARRACCINI, P..
BACKGROUND: Drought is a widespread limiting factor in coffee plants. It affects plant development, fruit production, bean development and consequently beverage quality. Genetic diversity for drought tolerance exists within the coffee genus. However, the molecular mechanisms underlying the adaptation of coffee plants to drought are largely unknown. In this study, we compared the molecular responses to drought in two commercial cultivars (IAPAR59, drought-tolerant and Rubi, drought-susceptible) of Coffea arabica grown in the field under control (irrigation) and drought conditions using the pyrosequencing of RNA extracted from shoot apices and analysing the expression of 38 candidate genes. RESULTS: Pyrosequencing from shoot apices generated a total of 34.7...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Candidate gene; Coffee; Differential gene expression; RNA-Seq; Real-time PCR (RT-qPCR); Drought; Coffea arabica.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1043710
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Identification of General Patterns of Sex-Biased Expression in Daphnia, a Genus with Environmental Sex Determination ArchiMer
Molinier, Cecile; Reisser, Celine; Fields, Peter; Segard, Adeline; Galimov, Yan; Haag, Christoph R..
Daphnia reproduce by cyclic-parthenogenesis, where phases of asexual reproduction are intermitted by sexual production of diapause stages. This life cycle, together with environmental sex determination, allow the comparison of gene expression between genetically identical males and females. We investigated gene expression differences between males and females in four genotypes of Daphnia magna and compared the results with published data on sex-biased gene expression in two other Daphnia species, each representing one of the major phylogenetic clades within the genus. We found that 42% of all annotated genes showed sex-biased expression in D. magna. This proportion is similar both to estimates from other Daphnia species as well as from species with genetic...
Tipo: Text Palavras-chave: Differential gene expression; Males; Females; Daphnia; ESD; Genetics of Sex.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00442/55322/56832.pdf
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RNA fingerprinting using RAP-PCR identifies an EBAF homologue mRNA differentially expressed in rat oviduct Biocell
Valdecantos,Pablo A.; Argañaraz,Martín E.; Abate,Carlos M.; Miceli,Dora C..
As a step towards the identification of genes preferentially expressed in the oviduct during early rat embryo development, we isolated a cDNA fragment (Pr14) by using RNA arbitrarily primed PCR (RAP-PCR), being its expression restricted to oviduct and uterus; its mRNA is mainly expressed in oviduct during late luteal phase and early pregnancy. This fragment is 100% identical to a rat DNA sequence (Accession No. NW_047400) downstream the terminal exon of a Rattus norvegicus gene (Locus Link Accession No. LOC289316) similar to ebaf (endometrial bleeding-associated factor), a novel member of the Transforming Growth Factor superfamily. Northern analyses showed that this sequence hybridizes with 2.9 kb and 4.1 kb mRNAs in early pregnant rat oviducts. However,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Differential gene expression; Oviduct; RNA fingerprint; CDNA.
Ano: 2004 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300005
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Transcript levels of ten caste-related genes in adult diploid males of Melipona quadrifasciata (Hymenoptera, Apidae): a comparison with haploid males, queens and workers Genet. Mol. Biol.
Borges,Andreia A.; Humann,Fernanda C.; Campos,Lucio A. Oliveira; Tavares,Mara G.; Hartfelder,Klaus.
In Hymenoptera, homozygosity at the sex locus results in the production of diploid males. In social species, these pose a double burden by having low fitness and drawing resources normally spent for increasing the work force of a colony. Yet, diploid males are of academic interest as they can elucidate effects of ploidy (normal males are haploid, whereas the female castes, the queens and workers, are diploid) on morphology and life history. Herein we investigated expression levels of ten caste-related genes in the stingless bee Melipona quadrifasciata, comparing newly emerged and 5-day-old diploid males with haploid males, queens and workers. In diploid males, transcript levels for dunce and paramyosin were increased during the first five days of adult...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bee; Real time PCR; Caste; Diploid male; Differential gene expression.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400025
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Transcriptome profiling of wild Arachis from water-limited environments uncovers drought tolerance candidate genes. Repositório Alice
BRASILEIRO, A. C. M.; MORGANTE, C. V.; ARAUJO, A. C. G.; LEAL-BERTIOLI, S. C. M.; SILVA, A. K.; MARTINS, A. C. Q.; VINSON, C. C.; SANTOS, C. M. R.; BONFIM, O.; TOGAWA, R. C.; SARAIVA, M. A. P.; BERTIOLI, D. J.; GUIMARAES, P. M..
Peanut (Arachis hypogaeaL.) is an important le-gume cultivated mostly in drought-prone areas where its pro-ductivity can be limited by water scarcity. The development of more drought-tolerant varieties is, therefore, a priority for pea-nut breeding programs worldwide. In contrast to cultivated peanut, wild relatives have a broader genetic diversity and constitute a rich source of resistance/tolerance alleles to biotic and abiotic stresses. The present study takes advantage of this diversity to identify drought-responsive genes by analyzing the expression profile of two wild species, Arachis duranensis and Arachis magna (AA and BB genomes, respectively), in response to progressive water deficit in soil. Data analysi from leaves and roots of A. duranensis...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Amendoim; Resistência a seca; Gene tolerante; Cultura resistente a seca; Biologia molecular; SSHlibraries; 454 Sequencing; Differential gene expression; Dry-down; Peanut wild relatives; QRT-PCR.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035448
Registros recuperados: 11
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