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Registros recuperados: 22
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Acute administration of fenproporex increased acetylcholinesterase activity in brain of young rats Anais da ABC (AABC)
TEODORAK,BRENA P.; FERREIRA,GABRIELA K.; SCAINI,GISELLI; WESSLER,LETÍCIA B.; HEYLMANN,ALEXANDRA S.; DEROZA,PEDRO; VALVASSORI,SAMIRA S.; ZUGNO,ALEXANDRA I.; QUEVEDO,JOÃO; STRECK,EMILIO L..
Fenproporex is the second most commonly amphetamine-based anorectic consumed worldwide; this drug is rapidly converted into amphetamine, in vivo, and acts by increasing dopamine levels in the synaptic cleft. Considering that fenproporex effects on the central nervous system are still poorly known and that acetylcholinesterase is a regulatory enzyme which is involved in cholinergic synapses and may indirectly modulate the release of dopamine, the present study investigated the effects of acute administration of fenproporex on acetylcholinesterase activity in brain of young rats. Young male Wistar rats received a single injection of fenproporex (6.25, 12.5 or 25mg/kg i.p.) or vehicle (2% Tween 80). Two hours after the injection, the rats were killed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acetylcholinesterase; Amphetamine; Dopamine; Fenproporex.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301389
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APLICACIÓN DEL TEST DE LA BARRA TRANSVERSAL MODIFICADO PARA EVALUAR RATAS HEMIPARKINSONIZADAS. Acta biol.Colomb.
BLANCO LEZCANO,LISETTE; LORIGADOS PEDRE,LOURDES DEL C.; FERNÁNDEZ VERDECIA,CARIDAD I; SERRANO SÁNCHEZ,TERESA; PAVÓN FUENTES,NANCY; FRANCIS TURNER,LILIANA.
La degeneración nigroestriatal que caracteriza a la enfermedad de Parkinson (EP) es estudiada en modelos experimentales en roedores por inyección de 6-hidroxidopamina (6-OHDA). El presente estudio presenta una versión modificada del test de la barra transversal (TBT) que permite la cuantificación del déficit motor a través de: tiempo que demora la rata en alcanzar una de las plataformas (latencia de escape, LE); tiempo que demora en caer de la barra (latencia de caída, LC); número total de errores cometidos durante la ejecución en cada barra (número de errores, NE). La forma y el diámetro de la sección transversal de la barra se modificaron desde barras rectangulares y circulares de 2,5 cm de diámetro hasta barras con esta misma forma y 1 cm de diámetro...
Tipo: Journal article Palavras-chave: 6-hydroxydopamina; Test de la barra transversal; Dopamine; Enfermedad de Parkinson.
Ano: 2010 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2010000200013
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Changes in the biogenic amine content of the prefrontal cortex, amygdala, dorsal hippocampus, and nucleus accumbens of rats submitted to single and repeated sessions of the elevated plus-maze test BJMBR
Carvalho,M.C.; Albrechet-Souza,L.; Masson,S.; Brandão,M.L..
It has been demonstrated that exposure to a variety of stressful experiences enhances fearful reactions when behavior is tested in current animal models of anxiety. Until now, no study has examined the neurochemical changes during the test and retest sessions of rats submitted to the elevated plus maze (EPM). The present study uses a new approach (HPLC) by looking at the changes in dopamine and serotonin levels in the prefrontal cortex, amygdala, dorsal hippocampus, and nucleus accumbens in animals upon single or double exposure to the EPM (one-trial tolerance). The study involved two experiments: i) saline or midazolam (0.5 mg/kg) before the first trial, and ii) saline or midazolam before the second trial. For the biochemical analysis a control group...
Tipo: Info:eu-repo/semantics/article Palavras-chave: One-trial tolerance; Dopamine; Serotonin; Prefrontal cortex; Amygdala; Hippocampus.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200014
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Combined effects of diethylpropion and alcohol on locomotor activity of mice: participation of the dopaminergic and opioid systems BJMBR
Gevaerd,M.S.; Sultowski,E.T.; Takahashi,R.N..
The widespread consumption of anorectics and combined anorectic + alcohol misuse are problems in Brazil. In order to better understand the interactive effects of ethanol (EtOH) and diethylpropion (DEP) we examined the locomotion-activating effects of these drugs given alone or in combination in mice. We also determined whether this response was affected by dopamine (DA) or opioid receptor antagonists. A total of 160 male Swiss mice weighing approximately 30 g were divided into groups of 8 animals per group. The animals were treated daily for 7 consecutive days with combined EtOH + DEP (1.2 g/kg and 5.0 mg/kg, ip), EtOH (1.2 g/kg, ip), DEP (5.0 mg/kg, ip) or the control solution coadministered with the DA antagonist haloperidol (HAL, 0.075 mg/kg, ip), the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diethylpropion; Ethanol; Locomotor activity; Dopamine; Opioid; Mice.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200015
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Dopaminergic modulation of grooming behavior in virgin and pregnant rats BJMBR
Serafim,A.P.; Felicio,L.F..
Dopamine receptors are involved in the expression of grooming behavior. The pregnancy-induced increase in self-licking observed in rats is important for mammary gland development and lactation. This study focuses on the role of dopamine receptor subtypes in grooming behavior of virgin and pregnant female rats. General and mammary gland grooming were measured in virgin rats treated with 0.25 mg/kg of the D1-like agonist SKF-81297 and antagonist SKF-83566 and the D2-like agonist lisuride and antagonist sulpiride. The effects of 0.01 and 0.25 mg/kg doses of the same agonists and antagonists were evaluated in pregnant rats as well. In virgin animals both SKF-83566 and sulpiride treatments significantly reduced the time spent in general grooming, while none of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Self-licking; Lisuride; SKF-81297; SKF-83566; Sulpiride; Mammary gland; Prolactin; Dopamine.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100015
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Dopaminergic profile of new heterocyclic N-phenylpiperazine derivatives BJMBR
Neves,G.; Fenner,R.; Heckler,A.P.; Viana,A.F.; Tasso,L.; Menegatti,R.; Fraga,C.A.M.; Barreiro,E.J.; Dalla-Costa,T.; Rates,S.M.K..
Dopamine constitutes about 80% of the content of central catecholamines and has a crucial role in the etiology of several neuropsychiatric disorders, including Parkinson's disease, depression and schizophrenia. Several dopaminergic drugs are used to treat these pathologies, but many problems are attributed to these therapies. Within this context, the search for new more efficient dopaminergic agents with less adverse effects represents a vast research field. The aim of the present study was to report the structural design of two N-phenylpiperazine derivatives, compound 4: 1-[1-(4-chlorophenyl)-1H-4-pyrazolylmethyl]-4-phenylhexahydropyrazine and compound 5: 1-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4-ylmethyl]-4-phenylhexahydropyrazine, planned to be dopamine...
Tipo: Info:eu-repo/semantics/other Palavras-chave: N-phenylpiperazine derivatives; Dopamine; Apomorphine hypothermia; Amphetamine stereotypy; Catalepsy.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500010
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Effect of one-week ethanol treatment on monoamine levels and dopaminergic receptors in rat striatum BJMBR
Vasconcelos,S.M.M.; Macêdo,D.S.; Lima,L.O.; Sousa,F.C.F.; Fonteles,M.M.F.; Viana,G.S.B..
We studied the effects of ethanol on the levels of norepinephrine, dopamine, serotonin (5-HT) and their metabolites as well as on D1- and D2-like receptors in the rat striatum. Ethanol (2 or 4 g/kg, po) was administered daily by gavage to male Wistar rats and on the 7th day, 30 min or 48 h after drug administration, the striatum was dissected for biochemical assays. Monoamine and metabolite concentrations were measured by HPLC and D1- and D2-like receptor densities were determined by binding assays. Scatchard analyses showed decreases of 30 and 43%, respectively, in D1- and D2-like receptor densities and no change in dissociation constants (Kd) 48 h after the withdrawal of the dose of 4 g/kg. Ethanol, 2 g/kg, was effective only on the density of D2-like...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rat striatum; Dopamine; Serotonin; Alcohol ingestion; Noradrenaline; D1 and D2 receptor density; Kd.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400013
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Effect of Sapindus trifoliatus on hyperalgesic in vivo migraine models BJMBR
Arulmozhi,D.K.; Veeranjaneyulu,A.; Bodhankar,S.L.; Arora,S.K..
Phytotherapies have offered alternative sources of therapy for migraine and gained much importance in prophylactic treatment. Sapindus trifoliatus is a medium-sized deciduous tree growing wild in south India that belongs to the family Sapindaceae. The pericarp is reported for various medicinal properties. A thick aqueous solution of the pericarp is used for the treatment of hemicrania, hysteria or epilepsy in folklore medicine. We have investigated the antihyperalgesic effects of the lyophilized aqueous extract of S. trifoliatus in animal models predictive of experimental migraine models using morphine withdrawal-induced hyperalgesia on the hot-plate test and on 0.3% acetic acid-induced abdominal constrictions in adult male Swiss albino mice. The extract...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sapindus trifoliatus; Hyperalgesia; Migraine; Dopamine; D2 receptor; Morphine withdrawal.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300019
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Effect of sildenafil (Viagra®) on the genital reflexes of paradoxical sleep-deprived male rats BJMBR
Andersen,M.L.; Bignotto,M.; Tufik,S..
Since there is evidence that paradoxical sleep deprivation (PSD) elicits penile erection (PE) and ejaculation (EJ), and that the erectile response of rats is mediated by nitric oxide, the present study sought to extend the latter finding by assessing the effects of sildenafil on the genital reflexes of male Wistar rats subjected to PSD. We also determined the influence of sildenafil on hormone concentrations. In the first experiment, sildenafil at doses ranging from 0.08 to 0.32 mg/kg was administered intraperitoneally to rats that had been deprived of sleep for 4 days and to home cage controls (N = 8-10/group). The frequency of PE and EJ was measured for 60 min. PSD alone induced PE in 50% of the animals; however, a single injection of sildenafil did not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paradoxical sleep deprivation; Sildenafil; Genital reflexes; Dopamine; Testosterone; Progesterone.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100008
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Effects of chronic ethanol treatment on monoamine levels in rat hippocampus and striatum BJMBR
Vasconcelos,S.M.M.; Cavalcante,R.A.; Aguiar,L.M.V.; Sousa,F.C.F.; Fonteles,M.M.F.; Viana,G.S.B..
We studied the effects of ethanol on concentrations of noradrenaline (NE), dopamine (DA) and serotonin (5-HT) and their metabolites in rat hippocampus and striatum. Ethanol (2 or 4 g/kg, po, from a 20% aqueous solution) was administered daily to male Wistar rats (4-13 per group) for 30 days and animals were sacrificed 30 min or 48 h after the last administration. Monoamines were measured by HPLC and considered significant at P < 0.05. A 47% increase in 5-HT levels was observed in the hippocampus with 4 g/kg ethanol in the 30-min protocol. Ethanol (2 and 4 g/kg) decreased DA (2114.5 ± 126.4 and 1785.1 ± 234.2 ng/g wet tissue, respectively) and 3,4-dihydroxyphenylacetic acid (DOPAC, 1477.6 ± 132.1 and 1218.8 ± 271.7 ng/g wet tissue, respectively) levels,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hippocampus; Striatum; Ethanol; Noradrenaline; Dopamine; Serotonin.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200009
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Effects of cocaine, methamphetamine and modafinil challenge on sleep rebound after paradoxical sleep deprivation in rats BJMBR
Martins,R.C.S; Andersen,M.L; Shih,M.C; Tufik,S.
Sleep loss is both common and critically relevant to our society and might lead to the abuse of psychostimulants such as amphetamines, cocaine and modafinil. Since psychoactive substance abuse often occurs within a scenario of sleep deficit, the purpose of this investigation was to compare the sleep patterns of rats challenged with cocaine (7 mg/kg, ip), methamphetamine (7 mg/kg, ip), or modafinil (100 mg/kg, ip) subsequent to paradoxical sleep deprivation (PSD) for 96 h. Our results show that, immediately after 96 h of PSD, rats (10 per group) that were injected with a psychostimulant presented lower percentages of paradoxical sleep compared to those injected with saline (P < 0.01). Regarding slow wave sleep (SWS), rats injected with psychostimulants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sleep deprivation; Sleep; Cocaine; Methamphetamine; Modafinil; Rebound; Dopamine.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100011
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Genotoxic, neurotoxic and neuroprotective activities of apomorphine and its oxidized derivative 8-oxo-apomorphine BJMBR
Picada,J.N.; Roesler,R.; Henriques,J.A.P..
Apomorphine is a dopamine receptor agonist proposed to be a neuroprotective agent in the treatment of patients with Parkinson's disease. Both in vivo and in vitro studies have shown that apomorphine displays both antioxidant and pro-oxidant actions, and might have either neuroprotective or neurotoxic effects on the central nervous system. Some of the neurotoxic effects of apomorphine are mediated by its oxidation derivatives. In the present review, we discuss recent studies from our laboratory in which the molecular, cellular and neurobehavioral effects of apomorphine and its oxidized derivative, 8-oxo-apomorphine-semiquinone (8-OASQ), were evaluated in different experimental models, i.e., in vitro genotoxicity in Salmonella/microsome assay and WP2...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apomorphine; 8-Oxo-apomorphine- semiquinone; Dopamine; Genotoxicity; Neurotoxicity.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400001
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Gentle handling temporarily increases c-Fos in the substantia nigra pars compacta BJMBR
Santos,C.A.; Andersen,M.L.; Lima,M.M.S.; Tufik,S..
Dopaminergic neurotransmission is involved in the regulation of sleep. In particular, the nigrostriatal pathway is an important center of sleep regulation. We hypothesized that dopaminergic neurons located in substantia nigra pars compacta (SNpc) could be activated by gentle handling, a method to obtain sleep deprivation (SD). Adult male C57/BL6J mice (N = 5/group) were distributed into non-SD (NSD) or SD groups. SD animals were subjected to SD once for 1 or 3 h by gentle handling. Two experiments were performed. The first determined the activation of SNpc neurons after SD, and the second examined the same parameters after pharmacologically induced dopaminergic depletion using intraperitoneal reserpine (2 mg/kg). After 1 or 3 h, SD and NSD mice were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sleep deprivation; Dopamine; C-Fos; Substantia nigra pars compacta; Gentle handling of mice.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000015
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Memory-impairing effects of local anesthetics in an elevated plus-maze test in mice BJMBR
Blatt,S.L.; Takahashi,R.N..
Post-training intracerebroventricular administration of procaine (20 µg/µl) and dimethocaine (10 or 20 µg/µl), local anesthetics of the ester class, prolonged the latency (s) in the retention test of male and female 3-month-old Swiss albino mice (25-35 g body weight; N = 140) in the elevated plus-maze (mean ± SEM for 10 male mice: control = 41.2 ± 8.1; procaine = 78.5 ± 10.3; 10 µg/µl dimethocaine = 58.7 ± 12.3; 20 µg/µl dimethocaine = 109.6 ± 5.73; for 10 female mice: control = 34.8 ± 5.8; procaine = 55.3 ± 13.4; 10 µg/µl dimethocaine = 59.9 ± 12.3 and 20 µg/µl dimethocaine = 61.3 ± 11.1). However, lidocaine (10 or 20 µg/µl), an amide class type of local anesthetic, failed to influence this parameter. Local anesthetics at the dose range used did not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lidocaine; Procaine; Dimethocaine; Elevated plus-maze; Memory; Dopamine.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400013
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Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats BJMBR
Hermel,E.E.S.; Severino,G.S.; Cecconello,A.L.; Pereira,F.M.; Sanvitto,G.L.; Lucion,A.B..
Neonatal handling has long-lasting effects on behavior and stress reactivity. The purpose of the present study was to investigate the effect of neonatal handling on the number of dopaminergic neurons in the hypothalamic nuclei of adult male rats as part of a series of studies that could explain the long-lasting effects of neonatal stimulation. Two groups of Wistar rats were studied: nonhandled (pups were left undisturbed, control) and handled (pups were handled for 1 min once a day during the first 10 days of life). At 75-80 days, the males were anesthetized and the brains were processed for immunohistochemistry. An anti-tyrosine hydroxylase antibody and the avidin-biotin-peroxidase method were used. Tyrosine hydroxylase-immunoreactive (TH-IR) neurons were...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Stress; Neonatal stimulation; Dopamine; Immunohistochemistry; Hypothalamus; Tyrosine hydroxylase.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900013
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Nocardia otitidiscaviarum (GAM-5) induces parkinsonian-like alterations in mouse BJMBR
Díaz-Corrales,F.J.; Colasante,C.; Contreras,Q.; Puig,M.; Serrano,J.A.; Hernández,L.; Beaman,B.L..
Parkinson's disease, a major neurodegenerative disorder in humans whose etiology is unknown, may be associated with some environmental factors. Nocardia otitidiscaviarum (GAM-5) isolated from a patient with an actinomycetoma produced signs similar to Parkinson's disease following iv injection into NMRI mice. NMRI mice were infected intravenously with a non-lethal dose of 5 x 10(6) colony forming units of N. otitidiscaviarum (GAM-5). Fourteen days after bacterial infection, most of the 60 mice injected exhibited parkinsonian features characterized by vertical head tremor, akinesia/bradykinesia, flexed posture and postural instability. There was a peak of nocardial growth in the brain during the first 24 h followed by a decrease, so that by 14 days nocardiae...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nocardia otitidiscaviarum (GAM-5); Parkinson's disease; Substantia nigra; Bradykinesia; Dopamine; Tyrosine hydroxylase.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400011
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One-pot synthesis of graphene-chitosan nanocomposite modified carbon paste electrode for selective determination of dopamine Electron. J. Biotechnol.
Liu,Chang; Zhang,Jing; Yifeng,E; Yue,Jingli; Chen,Lianshan; Li,Donghui.
Background A simple, rapid, low-cost and environmentally friendly method was developed to determine dopamine (DA) in the presence of ascorbic (AA) and uric acid (UA) based on a novel technique to prepare a graphene-chitosan (GR-CS) nanocomposite and modify it on the surface of carbon paste electrode (CPE). For our design, CS acts as a media to disperse and stabilize GR, and then GR plays a key role to selective and sensitive determination of DA. Results Under physiological conditions, the linear range for dopamine was determined from 1 × 10- 4 to 2 × 10- 7 mol/L with a good correlation coefficient of 0.9961 in the presence of 1000-fold interference of AA and UA. The detection limit was estimated to be 9.82 × 10- 8 mol/L (S/N = 3). In order to study the...
Tipo: Journal article Palavras-chave: Dopamine; Selective determination; Surface modification of carbon paste electrode; Technique of one-pot synthesis.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400007
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Paradoxical sleep deprivation: neurochemical, hormonal and behavioral alterations. Evidence from 30 years of research Anais da ABC (AABC)
Tufik,Sergio; Andersen,Monica L.; Bittencourt,Lia R. A.; Mello,Marco T. de.
Sleep comprises approximately one-third of a person's lifetime, but its impact on health and medical conditions remains partially unrecognized. The prevalence of sleep disorders is increasing in modern societies, with significant repercussions on people's well-being. This article reviews past and current literature on the paradoxical sleep deprivation method as well as data on its consequences to animals, ranging from behavioral changes to alterations in the gene expression. More specifically, we highlight relevant experimental studies and our group's contribution over the last three decades.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sleep; Sleep deprivation; Rebound; Dopamine; Erection; Corticosterone; Memory.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300016
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Prolactin induces adrenal hypertrophy BJMBR
Silva,E.J.; Felicio,L.F.; Nasello,A.G.; Zaidan-Dagli,M.; Anselmo-Franci,J.A..
Although adrenocorticotropic hormone is generally considered to play a major role in the regulation of adrenal glucocorticoid secretion, several reports have suggested that other pituitary hormones (e.g., prolactin) also play a significant role in the regulation of adrenal function. The aim of the present study was to measure the adrenocortical cell area and to determine the effects of the transition from the prepubertal to the postpubertal period on the hyperprolactinemic state induced by domperidone (4.0 mg kg-1 day-1, sc). In hyperprolactinemic adult and young rats, the adrenals were heavier, as determined at necropsy, than in the respective controls: adults (30 days: 0.16 ± 0.008 and 0.11 ± 0.007; 46 days: 0.17 ± 0.006 and 0.12 ± 0.008, and 61 days:...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pituitary; Domperidone; Dopamine; Rats; Morphometry.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200005
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Reproductive experience influences grooming behavior during pregnancy in rats BJMBR
Serafim,A.P.; Felicio,L.F..
The pregnancy-induced increase in self-licking observed in rats is important for mammary gland development and lactation. Reproductive experience has epidemiologial implications such as a decrease in the incidence of mammary gland cancer in women and it also influences various behavioral, neurochemical and endocrine parameters. The aim of the present study was to investigate the influence of reproductive experience on grooming behavior patterns during pregnancy in rats. Self-grooming behavior was measured in age-matched virgin, primi- and multigravid (days 7, 8, 9, 19, and 20 of pregnancy) rats. General grooming (head, forelimbs and shoulders) was not significantly different among virgin, primi- and multigravid rats during pregnancy. Confirming previous...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Self-licking; Parity; Lactation; Mammary gland; Prolactin; Dopamine.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300016
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