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A selected set of est-derived microsatellites, polymorphic and transferable across 6 species of eucalyptus. Repositório Alice
FARIA, D. A. de; MAMANI, E. M. C.; PAPPAS, M. de C. R.; PAPPAS JUNIOR, G. J.; GRATTAPAGLIA, D..
Tipo: Separatas Palavras-chave: EST-SSR; Tropical trees; Eucalyptus.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/871624
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EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.) Electron. J. Biotechnol.
Song,Guo Qi; Li,Meng Jun; Xiao,Han; Wang,Xing Jun; Tang,Rong Hua; Xia,Han; Zhao,Chuan Zhi; Bi,Yu Ping.
Making use of the gene resources of wild type peanuts is a way to increase the genetic diversity of the cultivars. Marker assisted selection (MAS) could shorten the process of inter-specific hybridization and provide a possible way to remove the undesirable traits. However, the limited number of molecular markers available in peanut retarded its MAS process. We started a peanut ESTs (Expressed Sequence Tags) project aiming at cloning genes with agronomic importance and developing molecular markers. In this study we found 610 ESTs that contained one or more SSRs from 12,000 peanut ESTs. The most abundant SSRs in peanut are trinucleotides (66.3%) SSRs and followed by dinucleotide (28.8%) SSRs. AG/TC (10.7%) repeat was the most abundant and followed by CT/GA...
Tipo: Journal article Palavras-chave: Arachis hypogaea L.; Cultivars; EST-SSR; Polymorphism; Wild-type peanut.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300007
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Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map Electron. J. Biotechnol.
Talia,Paola; Nishinakamasu,Verónica; Hopp,Horacio Esteban; Heinz,Ruth Amelia; Paniego,Norma.
In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the identification and characterization of 1,134 simple sequence repeat (SSR) containing expressed sequence tags (EST) from unigenes available databases. Twelve of these functional markers as well as 41 public SSR markers were successfully localized in linkage groups, thus contributing to the saturation of specific regions on a reference genetic-linkage-map derived from recombinant inbred lines (RIL) mapping population from the cross between PAC2 x RHA266 lines. The...
Tipo: Journal article Palavras-chave: EST-SSR; InDels; Linkage map; SSR; Sunflower.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600007
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Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee PAB
Pereira,Gabriella Santos; Padilha,Lilian; Pinho,Edila Vilela Resende Von; Teixeira,Rita de Kássia Siqueira; Carvalho,Carlos Henrique Siqueira de; Maluf,Mirian Peres; Carvalho,Bruna Line de.
The objective of this work was to analyze coffee (Coffea arabica) genotypes resistant to the coffee leaf miner (Leucoptera coffeella) using microsatellite markers. Sixty-six loci were evaluated, of which 63 were obtained from the Brazilian Coffee Expressed Sequence Tag (EST) database. These loci were amplified in bulks of individuals from F5 progenies of 'Siriema' (C. arabica x C. racemosa) resistant and susceptible to the insect, in eight samples of C. racemosa, and in a F6 population of 'Siriema' with 91 individuals segregating for resistance to the leaf miner. Polymorphisms were verified for two simple sequence repeat (SSR) loci in bulks of the susceptible progenies. The two polymorphic alleles were present in around 70% of the susceptible genotypes in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea arabica; Leucoptera coffeella; EST-SSR; Genotypes.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011001200010
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