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Low level of pollen-mediated gene flow from cultivated to wild grapevine: consequences for the evolution of the endangered subspecies Vitis vinifera L. subsp. silvestris Inra
Di Vecchi-Staraz, M.; Laucou, V.; Bruno, G.; Lacombe, T.; Gerber, S.; Bourse, T.; Boselli, M.; This, P..
A parentage and a paternity-based approach were tested for estimation of pollen-mediated gene flow in wild grapevine (Vitis vinifera L. subsp. silvestris), a wind-pollinated species occurring in Mediterranean Europe and southwestern Asia. For this purpose, 305 seedlings collected in 2 years at 2 locations in France from 4 wild female individuals and 417 wild individuals prospected from France and Italy were analyzed using 20 highly polymorphic microsatellite loci. Their profiles were compared with a database consisting of 3203 accessions from the Institut National de la Recherche Agronomique Vassal collection including cultivars, rootstocks, interspecific hybrids, and other wild individuals. Paternity was assigned for 202 (66.2%) of the 305 seedlings,...
Tipo: Journal Article Palavras-chave: ANALYSE DE PATERNITÉ; DISPERSION DU POLLEN; PLANTE TRANSGÉNIQUE; VIGNE SAUVAGE; MICROSATELLITE; EXTRACTION D'ADN PATERNITY ANALYSIS; POLLEN DISPERSAL; ROOTSTOCK; SEEDLING; TRANSGENIC PLANT; TRUE-TO-TYPE.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20097040a34d&uri=/notices/prodinra1/2009/03/
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