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| Registros recuperados: 75 | |
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| TEIXEIRA, A. V. C.; ELOY, A. M. X.; FURTADO, J. R.; PINHEIRO, R. R.; PONTES, M. S.. |
| The lack of information about seasonal variation of seminal plasma proteins in goats from Northeast Brazil led us to investigate the protein band distribution throughout the year. The experiment lasted one year (from April 2006 to March 2007) in which 230 semen collections from five adult goats (Capra hircus) were carried out, at ages ranging from 18 to 22 months, weighing 42.0 ± 10.0 kg, belonging to Anglo Nubian breed. A pool of seminal plasma samples from all the animals in each month of the year was submitted to 1D electrophoresis SDS-PAGE, 12.5%. The gels were analyzed through Life Science Software from UVP, Inc®. The correlation between air humidity and total seminal plasma proteins was not significant (r = -0.24; P > 0.05), while a moderate and... |
| Tipo: Artigo de periódico |
Palavras-chave: Plasma seminal; Raça Anglo-Nubiana; Caprino; Sêmen; Eletroforese; Variação sazonal; Goats; Electrophoresis; Seasonal variation.. |
| Ano: 2009 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/853159 |
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| VASCONCELOS, F. C.. |
| Resumo: Estudos realizados atualmente buscam identificar nos animais domésticos marcadores moleculares proteicos de fertilidade, de modo a contribuir para os programas de melhoramento genético, embora poucos ainda sejam realizados em ovinos deslanados, como os da raça Morada Nova. Portanto, este trabalho teve como objetivo identificar possíveis proteínas seminais relacionadas à fertilidade de ovinos da raça Morada Nova, utilizando-se a técnica de Eletroforese Bidimensional (2DE). Seis reprodutores da raça Morada Nova, na faixa etária de quatro a seis anos e peso médio de 42 kg foram submetidos à coleta de sêmen, antes da estação de monta, para obtenção do plasma seminal e, posterior, análise eletroforética. As estações de monta tiveram duração de 45 dias e... |
| Tipo: Teses |
Palavras-chave: Raça Morada Nova; Eletroforese bidimensional; 2DE electrophoresis; Ovino; Melhoramento genético animal; Fertilidade animal; Sêmen; Sheep; Electrophoresis; Genetic markers; Reproductive performance; Proteomics; Animal fertility; Seminal plasma. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076082 |
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| SOUZA, R. S. C. de; TONON, R. V.; STEPHAN, M. P.; SILVA, C. M.; PENTEADO, A. L.; CABRAL, L. M. C.; KUROZAWA, L. E.. |
| Resumo: O objetivo do trabalho foi avaliar as atividades antioxidante e antimicrobiana de hidrolisados proteicos de soro de leite obtidos por diferentes proteases (Alcalase e Flavourzyme) e sua mistura (proporção 50:50). Previamente à hidrólise enzimática, o soro de leite foi concentrado por ultrafiltração com membranas cerâmicas tubulares. A reação enzimática foi realizada de acordo com o método pH-stat, sendo coletadas alíquotas com diferentes graus de hidrólise ( GH), ao longo de cada hidrólise proteica. Os hidrolisados foram analisados quanto a estimativa do teor de peptídeos, atividade antioxidante pelos métodos de captura do radical livre ABTS+ e do radical peroxila (Oxygen radical antioxidant capacity - ORAC), eletroforese e atividade... |
| Tipo: Artigo de periódico |
Palavras-chave: Hidrólise enzimática; Grau de hidrólise; Capacidade antioxidante; Degree of hydrolysis; Soro de Leite; Proteína; Antioxidante; Proteína Hidrolisada; Peptídeo; Eletroforese; Peptides; Whey; Enzymatic hydrolysis; Antioxidant activity; Electrophoresis. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115788 |
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| Bach,E.E.; Alcântara,V.B.G.; Alcântara,P.B.; Veasey,E.A.. |
| This study characterized seven Pennisetum purpureum varieties, namely v. Anão, Bajra, Cameroon, Guaçu, Roxo, Taiwan A-144 and Uruckwami, through biochemical analyses, including protein, glucose and fructose contents, and polyacrylamide gel electrophoresis using the esterase system, by sampling 30, 60, 90, 120 and 150 day-old leaves. The number of nodes per stem and the percentage of bud emergence were also recorded. Variety Taiwan A-144 presented the highest number of nodes per stem and percentage of emerging buds. Protein concentration decreased gradually after 60 days for all varieties, except for Anão. Variety Guaçu presented the highest level of glucose in 90 day-old plants, whereas Cameroon presented the highest levels at 120 and 150 days. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Electrophoresis; Reducing sugars; Proteins; Elephant grass; Pennisetum purpureum. |
| Ano: 1995 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161995000300019 |
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| Varenne, A; Gareil, P; Colliec-jouault, Sylvia; Daniel, R. |
| The interaction of proteins with polysaccharides represents a major and challenging topic in glycobiology, since such complexes mediate fundamental biological mechanisms. An affinity capillary electrophoresis method has been developed to evidence the complex formation and to determine the binding properties between an anticoagulant polysaccharide of marine origin, fucoidan, and a potential target protein, antithrombin. This method is a variant of zonal electrophoresis in the mobility shift format. A fixed amount of protein was injected into a capillary filled with a background electrolyte containing the polysaccharide in varying concentrations. The effective mobility data of the protein were processed according to classical linearization treatments to... |
| Tipo: Text |
Palavras-chave: Antithrombin; Fucoidan; Electrophoresis; Protein interaction; Polysaccharides. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-2114.pdf |
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| PINTO, T. M. F.. |
| Estabelecer o perfil proteômico de uma determinada raça utilizando células espermáticas é um importante mecanismo para identificar biomarcadores e esclarecer o potencial reprodutivo e/ou econômico. Com a utilização da eletroforese bidimensional, além de detectar o conjunto de proteínas expressas de uma raça, também é possível, quando associada a ferramentas de bioinformática, estabelecer um comparativo quantitativo e qualitativo das proteínas expressas dos grupos em estudos. O objetivo neste trabalho é caracterizar o perfil proteômico do espermatozóide de caprinos da raça Saanen e fazer uma análise comparativa com a raça Moxotó correlacionando biomarcadores de fertilidade que são exclusivas para cada raça. Foram utilizados cinco reprodutores para cada raça... |
| Tipo: Teses |
Palavras-chave: Eletroforese biodimensional 2-DE; Caprino; Reprodução animal; Goats; Reproduction; Electrophoresis; Spermatozoa; Proteomics. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1088706 |
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| SCHMIDT,Michele Mantelli; FONTOURA,Andrine Menna da; VIDAL,Alessandra Roseline; DORNELLES,Rosa Cristina Prestes; KUBOTA,Ernesto Hashime; MELLO,Renius de Oliveira; CANSIAN,Rogério Luis; DEMIATE,Ivo Mottin; OLIVEIRA,Cristina Soltovski de. |
| Abstract Obtaining collagen and hydrolysates from mechanically separated chicken meat residue is an excellent way to add value to this waste. The aims of this study were to obtain collagen hydrolysates from chicken MSM residue using Alcalase® and Flavourzyme® enzymes, as well characterizing the functional, structural and thermal stability properties. The highest degree of hydrolysis was obtained using Alcalase®, 36.11%, while using Flavourzyme® resulted in 12.02%. The DSC analysis of the collagen indicated a denaturation temperature of 46.47 °C. The FTIR spectra of the crude collagen showed absorption peaks that were characteristic of amide bands A, B, I, II and III. In the spectra of the hydrolysed the area of the amide bands was reduced, and some peaks... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alcalase®; Electrophoresis; Flavourzyme®; FTIR; Functional properties. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020005006203 |
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| Erdeş,Efe; Doğan,Tuğba Somay; Coşar,İlhan; Danışman,Tarık; Kunt,Kadir Boğaç; Şeker,Tamay; Yücel,Meral; Özen,Can. |
| Background Scorpion venoms are rich bioactive peptide libraries that offer promising molecules that may lead to the discovery and development of new drugs.Leiurus abdullahbayrami produces one of the most potent venoms among Turkish scorpions that provokes severe symptoms in envenomated victims.Methods In the present study, the peptide profile of the venom was investigated by electrophoretic methods, size-exclusion and reversed-phase chromatography and mass spectroscopy. Cytotoxic and antimicrobial effects were evaluated on a breast cancer cell line (MCF-7) and various bacterial and fungal species.Results Proteins make up approximately half of the dry weight of L. abdullahbayrami crude venom. Microfluidic capillary electrophoresis indicated the presence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Scorpion venom; Toxin; Peptide; Leiurus abdullahbayrami; Microfluidic capillary; Electrophoresis; Peptidomics; Venomics; Cytotoxicity; Antimicrobial activity; Turkey. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200338 |
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| Collares,Eliana Antonia Silveira; Choer,Eva; Pereira,Arione da Silva. |
| The objective of this work was to characterize 27 potato genotypes, using molecular markers. Polyacrylamide gel electrophoresis, RAPD techniques and isozymes of esterase, phosphoglucomutase and soluble proteins were analyzed in tubers, and isocitrate dehydrogenase, aspartate transaminase, phosphoglucomutase and peroxidase, in leaves. Eighteen primers were tested and four were chosen, kits OPX (01, 04 and 09) and OPY (07), to analyze RAPD markers in leaf extracts. Similarity and cluster analysis were conducted using Jaccard coefficient and the unweighted pair-group method using arithmetic average. Despite the differences detected in the analysis of proteins and isozymes in the tubers, as well as of isozymes in the leaves, the characterization of all... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum tuberosum; Electrophoresis; Isozymes; RAPD; Genetic similarity. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000900006 |
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| BORGES, M. V.; ALVES, M. F.; CHAVES, M. A.; EGITO, A. S. do; GROSS, E.; FERRÃO, S. P. B.. |
| Abstract: The objective of this work was to obtain buffalo milk powder through the process of drying in mini spray dryer, and evaluate its physical, chemical, structural and proteomic features during storage. The buffalo milk was subjected to drying by atomization, air temperature of 140°C inlet. The samples were evaluated at times 0, 30, 60, 90, 120 and 150 days of storage. The moisture content, fat, protein, ash, density, acidity, solubility index, burning particles were analysed, along with structural analysis by Scanning Electron Microscopy (SEM) and proteomic study (SDS-PAGE). The production of milk powder from the set standards produced a buffalo milk powder with satisfactory results of physical and chemical compositions. In the SEM analysis was... |
| Tipo: Artigo de periódico |
Palavras-chave: Leite de búfala; Búfalo; Tecnologia de alimento; Microscopia; Eletroforese; Composição química; Atomização; Armazenamento; Buffalo milk; Microscopy; Electrophoresis; Food composition; Chemical composition; Spray drying; Storage; Food technology. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1069628 |
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| Registros recuperados: 75 | |
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