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FSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes. Repositório Alice
BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA FABJAN, J. M. G. de; ALFRADIQUE, V. A. P.; CORTÊS, L. R.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z..
Ovarian stimulation is an important tool to increase the number of oocytes obtained by laparoscopy for the in vitro production of embryos (IVP). In sheep, different concentrations of FSH administered in single dose (SD) or multiple doses (MD) have been adopted. In parallel, the oocyte quality is fundamental for IVP success, so strategies to produce more competent oocytes have been evaluated. The aim of this study was to evaluate the gene expression profile of BCB+ COC from different hormonal protocols of ovarian stimulation in Santa Inês ewes. To achieve that, a cross-over design was used, where 12 pluriparous ewes had their follicular wave synchronized (Balaro et al., Domest Anim Endocrinol, 54: 10-14, 2016). At 80 h after progestogen implant removal, all...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Expressão gênica; Produção de embriões in vitro; Ovino; Reprodução animal; Transferência de embrião; Sheep; Reproduction; In vitro embryo production; Ova; FSH; Embryo Technology; Gene expression.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074359
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Goat incubator: the doe as a life incubator of bovine oocytes - first step. Repositório Alice
BATISTA, R. I. T. P.; CAMARGO, L. S. de A.; SOUZA FABJAN, J. M. G.; PRATES, J. F.; TREVIZAN, J. T.; BRANDÃO, F. Z.; FONSECA, J. F. da.
Despite significant improvements in the in vitro production of cattle embryos, the suboptimal in vitro culture environment still limits the embryo quality and production. Techniques that associate the advantages of in vivo and in vitro systems, such as intrafollicular transfer of immature oocytes, have been proposed mainly to increase the embryo quality. In this context, we tried to use a goat as live incubator and associated nonsurgical embryo transfer techniques in small ruminants to perform ex situ (in vivo) maturation of bovine oocytes. For this, immature bovine cumulus-oocyte complexes (COCs) of grade 1 and 2 were randomly distributed into two groups for in vitro (IVM; n = 38) and ex situ (ESM; n = 40) maturation. The IVM was performed for a period of...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Non-surgical collection; Ovino; Ovelha; Reprodução animal; Tecnologia do embrião; Cultura In Vitro; Maturação artificial; Sheep; Ewes; In vitro fertilization; In vitro culture; Reproduction; Embryo Technology; Ova; Maturation.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074430
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The influence of different methods of frozen-thawed ovine spermatozoa selection on sperm capacitation and viability after incubation. Repositório Alice
OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G.; PENEIRAS, A. B. V.; BALARO, M. F. A.; FREITAS, V. J. F.; FONSECA, J. F. da; BRANDÃO, F. Z..
Abstract: Sperm capacitation is an essential event for fertilization; however, it decreases the sperm lifespan and viability. The aim of this study was to evaluate the effects of four sperm selection techniques on sperm capacitation and viability after incubation. A pool of frozen-thawed sperm from 10 Santa Inês rams was used. The samples were submitted to one of the following sperm selection techniques: sperm washing, Percoll gradient, mini-Percoll gradient, Swim-up and control group. At mini-Percoll technique, was used 400 microliters of 90% and 45% gradients and a centrifugation at 500 xg for 5 minutes. In Percoll, was used 1 mL of each gradient and a centrifugation at 700 xg for 10 minutos. During Swim-up, the sperm was incubated in 1 ml of SPERM-TALP...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Longevidade espermática; Seleção espermática; Raça Santa Inês; Sperm longevity; Sperm selection; Ovino; Carneiro; Reprodução animal; Transferência de Embrião; Sêmen; Espermatozóide; Sheep; Reproduction; Embryo Technology; Rams.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074502
Registros recuperados: 3
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