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Comparative embryotoxicity and genotoxicity of the herbicide diuron and its metabolites in early life stages of Crassostrea gigas: implication of reactive oxygen species production ArchiMer
Behrens, Daphne; Rouxel, Julien; Burgeot, Thierry; Akcha, Farida.
Herbicides are one of the major classes of pollutants contaminating coastal waters over the world. Among them, diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a phenylurea herbicide frequently detected in oyster-producing area, known to be toxic for this important exploited non-target species. With the aim to investigate the mechanisms by which diuron displays its toxicity in oyster, the implication of both biotransformation and oxygen reactive species (ROS) production was studied considering embryotoxicity and genotoxicity as endpoints. Comparative embryotoxicity and genotoxicity of diuron and its main metabolites (DCPMU, DCPU and 3,4-DCA) were thus studied on oyster larvae by the embryo-larval bioassay on D larvae and the comet assay on trochophore...
Tipo: Text Palavras-chave: Oyster; Herbicide; Embryotoxicity; Genotoxicity; Oxidative stress; Biotransformation.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00324/43521/43053.pdf
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Copper induces expression and methylation changes of early development genes in Crassostrea gigas embryos ArchiMer
Sussarellu, Rossana; Lebreton, Morgane; Rouxel, Julien; Akcha, Farida; Riviere, Guillaume.
Copper contamination is widespread along coastal areas and exerts adverse effects on marine organisms such as mollusks. In the Pacific oyster, copper induces severe developmental abnormalities during early life stages; however, the underlying molecular mechanisms are largely unknown. This study aims to better understand whether the embryotoxic effects of copper in Crassostrea gigas could be mediated by alterations in gene expression, and the putative role of DNA methylation, which is known to contribute to gene regulation in early embryo development. For that purpose, oyster embryos were exposed to 4 nominal copper concentrations (0.1, 1, 10 and 20 μg L−1 Cu2+) during early development assays. Embryotoxicity was monitored through the oyster embryo-larval...
Tipo: Text Palavras-chave: Oyster; Embryotoxicity; Copper; Gene expression; DNA methylation.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00417/52856/53756.pdf
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Development of a reference artificial sediment for chemical testing adapted to the MELA sediment contact assay ArchiMer
Le Bihanic, Florane; Perrichon, Prescilla; Landi, Laure; Clerandeau, Christelle; Le Menach, Karyn; Budzinski, Helene; Cousin, Xavier; Cachot, Jerome.
Most persistent organic pollutants, due to their hydrophobic properties, accumulate in aquatic sediments and represent a high risk for sediment quality. To assess the toxicity of hydrophobic pollutants, a novel approach was recently proposed as an alternative to replace, refine and reduce animal experimentation: the medaka embryo–larval sediment contact assay (MELAc). This assay is performed with Japanese medaka embryos incubated on a natural sediment spiked with the compound being tested. With the aim of improving this assay, our study developed a reference exposure protocol with an artificial sediment specifically designed to limit natural sediment composition uncertainties and preparation variability. The optimum composition of the new artificial...
Tipo: Text Palavras-chave: Artificial sediment; Spiked sediment; PAHs; Japanese medaka embryos; Embryotoxicity; Teratogenicity.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00187/29872/28479.pdf
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Developmental toxicity of PAH mixtures in fish early life stages. Part I: adverse effects in rainbow trout ArchiMer
Le Bihanic, Florane; Morin, Benedicte; Cousin, Xavier; Le Menach, Karyn; Budzinski, Helene; Cachot, Jerome.
A new gravel-contact assay using rainbow trout, Oncorhynchus mykiss, embryos was developed to assess the toxicity of polycyclic aromatic hydrocarbons (PAHs) and other hydrophobic compounds. Environmentally realistic exposure conditions were mimicked with a direct exposure of eyed rainbow trout embryos incubated onto chemical-spiked gravels until hatching at 10 °C. Several endpoints were recorded including survival, hatching delay, hatching success, biometry, developmental abnormalities, and DNA damage (comet and micronucleus assays). This bioassay was firstly tested with two model PAHs, fluoranthene and benzo[a]pyrene. Then, the method was applied to compare the toxicity of three PAH complex mixtures characterized by different PAH compositions: a pyrolytic...
Tipo: Text Palavras-chave: PAH mixture; Oil extract; Pyrolytic extract; Embryotoxicity; Developmental defects; Genotoxicity.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00188/29919/28384.pdf
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Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos. Repositório Alice
CAMPELO, I. S.; PEREIRA, A. F.; ALCÂNTARA-NETO, A. S.; CANEL, N. G.; SOUZA-FABJAN, J. M. G.; TEIXEIRA, D. I. A.; CAMARGO, L. S. de A.; MELO, L. M.; RÁDIS-BAPTISTA, G.; SALAMONE, D. F.; FREITAS, V. J. F..
2014
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cattle; Developmental competence; Embryotoxicity; Preimplantation embryo; QPCR.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1010619
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Genotoxicity of diuron and glyphosate in oyster spermatozoa and embryos ArchiMer
Akcha, Farida; Spagnol, Charlene; Rouxel, Julien.
We investigated the effects of genotoxicant exposure in gametes and embryos to find a possible link between genotoxicity and reproduction/developmental impairment, and explore the impact of chemical genotoxicity on population dynamics. Our study focused on the genotoxic effects of two herbicides on oyster gametes and embryos: glyphosate (both as an active substance and in the Roundup formulation) and diuron. France is Europe's leading consumer of agrochemical substances and as such, contamination of France's coastal waters by pesticides is a major concern. Glyphosate and diuron are among the most frequently detected herbicides in oyster production areas; as oyster is a specie with external reproduction, its gametes and embryos are in direct contact with...
Tipo: Text Palavras-chave: Genotoxicity; Embryotoxicity; Oyster; Gametes; Pesticides; Diuron; Glyphosate.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00070/18128/15783.pdf
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Investigating the relationship between embryotoxic and genotoxic effects of benzo[alpha]pyrene, 17 alpha-ethinylestradiol and endosulfan on Crassostrea gigas embryos ArchiMer
Wessel, Nathalie; Rousseau, Sabrina; Caisey, Xavier; Quiniou, Francoise; Akcha, Farida.
Genotoxicity biomarkers are widely measured in ecotoxicology as molecular toxic endpoints of major environmental pollutants. However, the long-term consequences of such damage still have to be elucidated. Some authors have suggested that the accumulation of unrepaired DNA lesions could explain the embryotoxicity of certain chemical pollutants. As embryotoxicity exerts a direct impact on the recruitment rate, genotoxicity could be closely related to disturbances of ecological concern and produce a possible impact upon population dynamics. The aim of the present work was to study the genotoxicity and the embryotoxicity of three relevant pollutants for oyster embryos: the polycyclic aromatic hydrocarbon, benzo[a]pyrene (BaP), the synthetic estrogenic...
Tipo: Text Palavras-chave: 17 alpha ethinylestradiol; BaP; Endosulfan; DNA damage; Embryotoxicity; Pacific oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4058.pdf
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Ketoconazole- and fluconazole-induced embryotoxicity and skeletal anomalies in wistar rats: a comparative study BABT
Amaral,Vanessa Cristiane de Santana; Nunes Junior,Guilhermino Pereira.
Ketoconazole and fluconazole are two broad-spectrum azole antifungals used for the treatment of superficial and systemic mycoses. Embryotoxicity and teratogenicity have been reported in some studies when those drugs are administered at high doses to pregnant rats. The aim of this study was to present a comparative study of embryotoxic effects as well as the skeletal anomalies in fetuses of Wistar rats which received ketoconazole and fluconazole at teratogenic doses on gestational days (GD) 6 through 15 (organogenesis period). On gestational day (GD) 21, the dams were euthanized and examined for standard parameters of reproductive outcome. Fetuses were stained with alizarin red and the bones of the head, trunk, forelimb and hindlimb were examined for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ketoconazole; Fluconazole; Maternal toxicity; Embryotoxicity; Skeletal malformations.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600010
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Relationships between fetal body weight of Wistar rats at term and the extent of skeletal ossification BJMBR
Chahoud,I.; Paumgartten,F.J.R..
We investigated the relationship between fetal body weight at term (pregnancy day 21) and the extent of ossification of sternum, metacarpus, metatarsus, phalanges (proximal, medial and distal) of fore- and hindlimbs and cervical and coccygeal vertebrae in Wistar rats. The relationships between fetal body weight and sex, intrauterine position, uterine horn, horn size, and litter size were determined using historical control data (7594 fetuses; 769 litters) of untreated rats. Relationships between body weight and degree of ossification were examined in a subset of 1484 historical control fetuses (154 litters) which were subsequently cleared and stained with alizarin red S. Fetal weight was independent of horn size, uterine horn side (left or right) or...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ossification centers; Fetal skeleton; Delayed fetal growth; Prenatal growth retardation; Malformation; Embryotoxicity.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400010
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Toxic Effects of Ochratoxin A on Calcium Metabolism during Chick Embryo Developmentand in Hatched Chicks Rev. Bras. Ciênc. Avic.
Elsayed,MAE; Hatab,MH; Elaroussi,MA.
ABSTRACT The present study was carried out to study the effect of in-ovo ochratoxin A (OTA) injection in Highline layer eggs on calcium metabolism and blood biochemical parameters of embryos and after the hatch. At day 10 of embryonic development, one hundred and sixty-two fertile eggs were individually weighed and divided into two equal treatments. The first treatment (control) consisted of the individual injection of fertile eggs with 50 µL sodium carbonate. In the second treatment (OTA), fertile eggs were individually injected with 12.5 ng OTA dissolved in 50 µL sodium carbonate. On days 12, 14, and 16 of incubation and at the hatch, serum calcium and inorganic phosphorus concentrations were lower (p<0.05), while sodium, alkaline phosphatase and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ochratoxicosis; Embryotoxicity; Blood electrolytes; Layer chickens.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300371
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Toxicity assessment of peptaibols and contaminated sediments on Crassostrea gigas embryos ArchiMer
Poirier, Laurence; Quiniou, Francoise; Ruiz, Nicolas; Montagu, Monique; Amiard, Jean-claude; Pouchus, Yves François.
Peptaibols are known membrane-modifying peptides that were recently detected in marine sediments and mussels collected from a shellfish farming area (Fier d'Ars, Atlantic coast, France). In this investigation, embryotoxicity bioassays with oysters (Crassostrea gigas) were performed to assess acute toxicity of alamethicin and different groups of peptaibols produced by a Trichoderma longibrachiatum strain isolated from marine environment. C gigas embryos appeared very sensitive to all the metabolites examined with higher toxic effects for long-sequence peptides (EC50 ranging from 10 to 64nM). D-shaped larvae with mantle abnormality were particularly noticed when peptaibol concentrations increased. Disturbances of embryogenesis were also observed following...
Tipo: Text Palavras-chave: Risk assessment; Embryotoxicity; Bivalve bioassay; Mycotoxins; Marine fungi.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2680.pdf
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Toxicology of Lapachol in rats: embryolethality BJB
GUERRA,M. de O.; MAZONI,A. S. B.; BRANDÃO,M. A. F.; PETERS,V. M..
Lapachol is a naphtoquinone with therapeutic potential against enterovirus, Chagas disease and is also used as an antimalarial and antiinflamatory agent. In order to study teratogenic potential of Lapachol, pregnant Wistar rats were treated with 0.5 ml of distilled water (control group); 0.5 ml of hydroalcoholic solution (vehicle group) and 10 mg of Lapachol in 0.5 ml of hydroalcoholic solution (treated group) by oral gavage from the 8th to the 12th day of pregnancy. The following variables were observed: maternal body weight on days 1, 6, l5 and 21 and food intake on days 2, 6, 15 and 21 of pregnancy. The number of live and dead fetuses and the sites of resorptions were counted. The ovaries were weighed and the corpora lutea were counted. Data were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lapachol; Embryotoxicity; Rat.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082001000100021
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Zebrafish exposure to graphene oxide is related to behavior changes. Repositório Alice
CLEMENTE, Z.; MARTINEZ, D. S. T.; OLIVEIRA, C. R. M.; MAURER-MORELLI, C. V.; NUNES, M. C. S.; CASTRO, V. L. S. S. de.
Graphene oxide (GO) has been explored to application in areas such as pharmaceutical and environmental sciences. However, GO safety is still unclear. Beyond that, environmental factors, as the presence of humic substances in aquatic environment, can affect nanomaterials behavior and its toxicological effects. The motor development of zebrafish embryo has been strongly characterized and established to neurotoxicity studies. A previous study of our group revealed that the exposure of zebrafish embryos to GO is related to a reduction in total body length and in acetylcholinesterase activity. Morphological and biochemical changes can be related to behavior abnormalities and affect organism?s survival, leading to serious environmental consequences. Thus, the...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Nanotoxicology; Embryotoxicity; Nanomaterials; Ecotoxicology.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1068076
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