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16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting BJM
Navarro-Noya,Yendi; Hernández-Rodríguez,César; Zenteno,Juan C; Buentello-Volante,Beatriz; Cancino-Díaz,Mario E; Jan-Roblero,Janet; Cancino-Díaz,Juan C.
Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophthalmitis; Staphylococcus epidermidis; PCR; DGGE.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100033
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Bilateral endogenous endophthalmitis associated with infective endocarditis: case report BJID
Arcieri,Enyr Saran; Jorge,Eduardo Ferreira; Ferreira,Lizane de Abreu; Fonseca,Maria B. da; Ferreira,Magno Antônio; Arcieri,Rafael Saran; Rocha,Flávio Jaime.
Bilateral endogenous endophthalmitis is a rare condition initiated by infection by microbes in the bloodstream, such as those arising from a foci of infective endocarditis. We report a case and discuss the diagnostic aspects and the clinical outcome of a patient with characteristic findings of the disease. The patient was a 49 year old white male who had a metallic aortic valve implanted 7 months previously, and who presented to the hospital with 10 days of fever, cough and dyspnea, then diarrhea and mental confusion. On the second day of hospitalization, he experienced sudden loss of vision in both eyes. A Gram-positive coccobacillus was isolated from the bloodstream, he was treated with fluoroquinolone with disappearance of fever, decreased ocular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial endocarditis; Endophthalmitis.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000600011
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