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Registros recuperados: 15
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Active anti-acetylcholinesterase component of secondary metabolites produced by the endophytic fungi of Huperzia serrata Electron. J. Biotechnol.
Wang,Zhejian; Ma,Zhao; Wang,Lili; Tang,Chengchen; Hu,Zhibi; Chou,GuiXin; Li,Wankui.
Background An endophytic fungus lives within a healthy plant during certain stages of, or throughout, its life cycle. Endophytic fungi do not always cause plant disease, and they include fungi that yield different effects, including mutual benefit, and neutral and pathogenic effects. Endophytic fungi promote plant growth, improve the host plant's resistance to biotic and abiotic stresses, and can produce the same or similar biologically active substances as the host. Thus, endophytic fungal products have important implications in drug development. Result Among the numerous endophytic fungi, we identified two strains, L10Q37 and LQ2F02, that have anti-acetylcholinesterase activity, but the active compound was not huperzine A. The aim of this study was to...
Tipo: Journal article Palavras-chave: Anti-acetylcholinesterase activity; Acetylcholinesterase inhibitor; Biological characteristics; Endophytic fungi.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600003
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Amylase production by endophytic fungi Cylindrocephalum sp. isolated from medicinal plant Alpinia calcarata (Haw.) Roscoe BJM
Sunitha.,V. H.; Ramesha.,A.; Savitha.,J.; Srinivas.,C.
Amylases are among the most important enzymes used in modern biotechnology particularly in the process involving starch hydrolysis. Fungal amylase has large applications in food and pharmaceutical industries. Considering these facts, endophytic fungi isolated from the plant Alpinia calcarata (Haw.) Roscoe were screened for amylolytic activity on glucose yeast extract peptone agar (GYP) medium. Among thirty isolates of endophytic fungi, isolate number seven identified as Cylindrocephalum sp. (Ac-7) showed highest amylolytic activity and was taken for further study. Influence of various physical and chemical factors such as pH, temperature, carbon and nitrogen sources on amylase production in liquid media were studied. The maximal amylase production was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpinia calcarata Roscoe; Endophytic fungi; Cylindrocephalum sp.; Amylase.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300049
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Biological activities of the fermentation extract of the endophytic fungus Alternaria alternata isolated from Coffea arabica L. BJPS
Fernandes,Maurette dos Reis Vieira; Silva,Tales Alexandre Costa e; Pfenning,Ludwig Heinrich; Costa-Neto,Cláudio Miguel da; Heinrich,Tassiela Andréa; Alencar,Severino Matias de; Lima,Marisa Aparecida de; Ikegaki,Masaharu.
A total of 22 endophytic fungi isolated from coffee (Coffea arabica L.) were cultivated in vitro and their crude extracts tested. The screening was carried out using the agar diffusion method against Staphylococcus aureus, Escherichia coli and Candida albicans. The most effective isolate was Alternaria alternata, and subsequently, its extract was assayed. The total phenolic content was 3.44 μg GAE/mg of the crude extract. For the antibacterial and antifungal activity assays, minimum inhibitory concentrations (MIC) and minimum bactericidal and fungicidal concentrations (MBC and MFC) were determined. The ranges of MIC values were 50-100 μg/mL for S. aureus and 400-800 μg/mL for E. coli. The extract did not show activity in the tested concentrations for C....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic fungi; Bioactive compounds; Alternaria alternata/extract/antioxidant activity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400010
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Biological properties of Endophytic Fungi BABT
Sudha,Venkatesan; Govindaraj,Ramar; Baskar,Kathirvelu; Al-Dhabi,Naif Abdullah; Duraipandiyan,Veeramuthu.
The endophytic fungus is have rich source of secondary metabolites which act as biological active agent in the higher plants. The fungal derivatives play vital part in human life and their compounds are the source of drug for cancer, microbial and viral diseases. The natural compounds from the endophytes acts as growth inhibitor of plant pathogenic organism. Endophytes are rich sources of natural products which are used in agriculture (plant growth and insecticidal), pharmaceutical industries and also used for phytoremediation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic fungi; Secondary metabolites; Anticancer; Phytoremediation; Pest management.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100413
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Chemical characterization of bioactive compounds from the endophytic fungus Diaporthe helianthi isolated from Luehea divaricata BJM
Specian,Vânia; Sarragiotto,Maria Helena; Pamphile,João Alencar; Clemente,Edmar.
Endophytic microorganisms, defined as fungi or bacteria that colonize the interior of plants without causing any immediate negative effects or damages, have reciprocal relationships with host plants. In some cases their presence is beneficial to the host due to the synthesis of bioactive compounds, among which several alcohols, esters, ketones and others that may react with other compounds and may be lethal to pathogenic microorganisms. Diaporthe helianthi (Phomopsis helianthi in its anamorphic phase) is available worldwide, especially in Europe, Asia and America. Isolated in Europe as an agent of the sunflower stem cancer, it has also been endophytically isolated from tropical and temperate plants. A D. helianthi strain isolated from Luehea divaricata has...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic fungi; Diaporthe helianthi; Secondary metabolite; Phenolic compound; Tyrosol.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300045
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Diversity and communities of culturable endophytic fungi from different tree peonies (geoherbs and non-geoherbs), and their biosynthetic potential analysis BJM
Yang,Guodong; Li,Peng; Meng,Lifen; Xv,Keyi; Dong,Faming; Qiu,Yan; He,Lei; Lin,Lin.
Abstract To know more about the potential roles of endophytic fungi in the formation mechanism of Daodi medicinal material, diversity and communities of culturable endophytic fungi in three types of tree peonies were investigated. Endophytic fungi of three types of tree peonies were isolated and identified. The diversity was analyzed. Bayesian trees constructed by MrBayes 3.2.6 after phylogenetic analysis of the ITS sequences. The endophytic fungi potential for synthesis of natural products was assessed by means of detecting NRPS and PKS gene sequences. In total, 364 endophytic fungi isolates representing 26 genera were recovered from Paeonia ostii ‘Feng Dan’, Paeonia ostii ‘Luoyang Feng Dan’, and Paeonia suffruticosa ‘Luoyang Hong’. More culturable...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic fungi; PKS; NRPS; Cortex Moutan; Daodi medicinal material.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500047
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Endophytic Fungi as Novel Resources of natural Therapeutics BABT
Rajamanikyam,Maheshwari; Vadlapudi,Varahalarao; amanchy,Ramars; Upadhyayula,Suryanarayana Murty.
ABSTRACT Fungal endophytes constitute a major part of the unexplored fungal diversity. Endophytic fungi (EF) are an important source for novel, potential and active metabolites. Plant-endophyte interaction and endophyte -endophyte interactions study provide insights into mutualism and metabolite production by fungi. Bioactive compounds produced by endophytes main function are helping the host plants to resist external biotic and abiotic stress, which benefit the host survival in return. These organisms mainly consist of members of the Ascomycota, Basidiomycota, Zygomycota and Oomycota. Recently, the genome sequencing technology has emerged as one of the most efficient tools that can provide whole information of a genome in a small period of time....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic fungi; Ascomycota; Natural products; Antidiabetic.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100430
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Endophytic fungi occurring in Ipomoea carnea tissues and their antimicrobial potentials BABT
Tayung,Kumanand; Sarkar,Meenakshi; Baruah,Paran.
The aim of this work was to study the endophytic fungi associated with the tissues of Ipomoea carnea, a common invasive plant of India. A total of 69 isolates belonging to ten taxa comprising 1.45% Zygomycetes, 10.14% Coelomycetes, 62.32% Hypomycetes, 18.84% sterile mycelia and 7.25% unidentified species were obtained. Species of Curvularia, Aspergillus, Fusarium, Colletotrichum and sterile fungus were isolated as dominant endophytes. Colonization frequency of Curvularia (7.25%) was highest which was isolated from all the tissues. The samples collected during the monsoon harbored more endophytes and showed higher species richness than the samples obtained in summer season. Of the total isolates, 15 isolates (21.74%) displayed antimicrobial activity,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ipomoea carnea; Endophytic fungi; Curvularia; Antimicrobial activity.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500003
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Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator BJM
Lisboa,Helen Cristina Fávero; Biasetto,Carolina Rabal; Medeiros,João Batista de; Araújo,Ângela Regina; Silva,Dulce Helena Siqueira; Teles,Helder Lopes; Trevisan,Henrique Celso.
A sensitive and efficient colorimetric method was optimized for detection of esterase enzymes produced by endophytic fungi for development of High-Throughput Screening (HTS). The fungi were isolated and obtained previously from plant species of Cerrado and Atlantic Forest located in areas of environmental preservation in the State of Sao Paulo / Brazil, as part of the project "Chemical and biological prospecting endophytic fungi associated to plant species of Cerrado and Atlantic Forest". The compounds ethyl butyrate, ethyl acetate and methyl propionate were used as standards esters which were hydrolyzed by extracellular enzyme from endophytic fungi (EC. 3.1.1.1 -carboxylesterases) for production of carboxylic acids. Thus, the reduction of the pH increases...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Esterase; PH indicator; Enzyme activity; Endophytic fungi.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300042
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Endophytic fungus Purpureocillium sp. A5 protect mangrove plant Kandelia candel under copper stress BJM
Gong,Bin; Liu,Guixiang; Liao,Rquan; Song,Jingjing; Zhang,Hong.
Abstract Mangrove is an important ecosystem in the world. Mangrove ecosystems have a large capacity in retaining heavy metals, and now they are usually considered as sinks for heavy metals. However, the mechanism of why the soil of mangrove ecosystems can retain heavy metal is not certain. In this research, endophytic fungus Purpureocillium sp. A5 was isolated and identified from the roots of Kandelia candel. When this fungus was added, it protected the growth of K. candel under Cu stress. This can be illustrated by analyzing chlorophyll A and B, RWC and WSD to leaves of K. candel. Purpureocillium sp. A5 reduces uptake of Cu in K. candel and changes the pH characterization of soil. Furthermore, A5 increase the concentration of Cu complexes in soil, and it...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic fungi; Purpureocillium lilacinum; Kandelia candel; Copper stress; Mangrove.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300530
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Endophytic fungus strain 28 isolated from Houttuynia cordata possesses wide-spectrum antifungal activity BJM
Pan,Feng; Liu,Zheng-Qiong; Chen,Que; Xu,Ying-Wen; Hou,Kai; Wu,Wei.
Abstract The aim of this paper is to identify and investigate an endophytic fungus (strain 28) that was isolated from Houttuynia cordata Thunb, a famous and widely-used Traditional Chinese Medicine. Based on morphological methods and a phylogenetic analysis of ITS sequences, this strain was identified as Chaetomium globosum. An antifungal activity bioassay demonstrated that the crude ethyl acetate (EtOAc) extracts of strain 28 had a wide antifungal spectrum and strong antimicrobial activity, particularly against Exserohilum turcicum (Pass.) Leonard et Suggs, Botrytis cinerea persoon and Botrytis cinerea Pers. ex Fr. Furthermore, the fermentation conditions, extraction method and the heat stability of antifungal substances from strain 28 were also studied....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic fungi; Houttuynia cordata Thunb.; Chaetomium globosum; Antifungal activity; Process parameter.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200480
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Fungi associated with Copaifera oblongifolia (Fabaceae) seeds: occurrence and possible effects on seed germination Acta Botanica
Fernandes,Elio Gomes; Valério,Henrique Maia; Duarte,Karen Luiza Rodrigues; Capuchinho,Lauany Matos de Novais; Fagundes,Marcílio.
ABSTRACT Copaifera oblongifolia is a recurrent plant species in degraded areas yet there is little information about its seed biology and interactions with fungi. The aims of this study were to identify the fungal community (endophytic and non-endophytic) associated with C. oblongifolia seeds and to test the effects of these different fungal species on germination. In general, germination of C. oblongifolia seeds was high (about 85 %), initiating four days after sowing and continuing for a total of 20 days. External asepsis with sodium hypochlorite did not influence germinability of C. oblongifolia. Seed infestation with Aspergillus niger, A. flavus, Mucor sp. and Rhizopus stolonifer did not affect the time required for germination, however, infection with...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Endophytic classification; Endophytic fungi; Saprophytic fungi; Seed germination; Seed-fungus interactions.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000100179
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Identification and colonization of endophytic fungi from soybean (Glycine max (L.) Merril) under different environmental conditions BABT
Pimentel,Ida Chapaval; Glienke-Blanco,Chirlei; Gabardo,Juarez; Stuart,Rodrigo Makowiecky; Azevedo,João Lúcio.
A total of 297 endophytic fungi were isolated from 1728 leaf and stem fragments collected about twenty and forty days after germination from soybean (Glycine max (L.) Merril) plants grown in the field and a greenhouse. The fungi belonged to eight groups, six dematiaceous genera (Alternaria, Cladosporium, Chaetomium, Curvularia, Drechslera and Scopulariopsis) and the non-dematiaceous genera Acremonium, Aspergillus, Colletotrichum, Fusarium, Paecilomyces and Penicillium along with some Mycelia sterilia.. There were qualitative and quantitative differences in the type and number of isolates obtained from greenhouse and field-grown plants, with more isolates being obtained from the latter. No difference was found in the number of fungi isolated from leaves and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic fungi; Soybean; Biological control.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600003
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Leishmanicidal, trypanocidal, and cytotoxic activities of endophytic fungi associated with bioactive plants in Brazil BJM
Rosa,Luiz H.; Gonçalves,Vívian N.; Caligiorne,Rachel B.; Alves,Tânia M. A.; Rabello,Ana; Sales,Policarbo A.; Romanha,Alvaro J.; Sobral,Marcos E. G.; Rosa,Carlos A.; Zani,Carlos L..
One hundred and twenty-one isolates of endophytic fungi were recovered from leaves of the bioactive Brazilian plant species Ageratum myriadenia, Palicourea tetraphylla, Piptadenia adiantoides, and Trixis vauthieri. All fungal isolates were cultivated in liquid media and crude extracts were obtained with ethyl acetate. The crude extracts were tested in bioassay panels using Leishmania amazonensis, Trypanosoma cruzi, the enzyme trypanothione reductase (TryR) from Trypanosoma cruzi, and three human cancer cell lines. Thirty-three extracts (27.2%) exhibited at least one biological activity. Seventeen extracts (14%) were cytotoxic against one or more human cancer cell line with the IC50 values ranged of >0.2 to 25 µg/mL. Twenty-four extracts (19.8%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic fungi; Human tumoral cell; Leishmania; Secondary metabolites; Trypanosoma cruzi.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200024
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The improvement of bioactive secondary metabolites accumulation in Rumex gmelini Turcz through co-culture with endophytic fungi BJM
Ding,Chang-hong; Wang,Qian-Bo; Guo,Shenglei; Wang,Zhen-yue.
Abstract Aspergillus sp., Fusarium sp., and Ramularia sp. were endophytic fungi isolated from Rumex gmelini Turcz (RGT), all of these three strains could produce some similar bioactive secondary metabolites of their host. However the ability to produce active components degraded significantly after cultured these fungi alone for a long time, and were difficult to recover. In order to obtain more bioactive secondary metabolites, the co-culture of tissue culture seedlings of RGT and its endophytic fungi were established respectively, and RGT seedling was selected as producer. Among these fungi, Aspergillus sp. showed the most significant enhancement on bioactive components accumulation in RGT seedlings. When inoculated Aspergillus sp. spores into media of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rumex gmelini Turcz (RGT); Endophytic fungi; Aspergillus sp.; Seedlings; Bioactive secondary metabolites.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200362
Registros recuperados: 15
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