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Amazonian isolates of Metarhizium are effective for killing Bactrocera carambolae (Diptera: Tephritidae) Repositório Alice
BRITO, B. D.; LIMA, A. L.; CRUZ, K. R.; BARIANI, A.; JESUS-BARROS, C. R. de; PEREIRA, J. F.; ADAIME, R..
Bactrocera carambolae Drew & Hancock is a quarantine pest present in Brazil and is the main phytosanitary barrier for the export of fresh fruits from that country. In this work, we evaluated the effect of Amazonian isolates of Metarhizium on kill larvae, pupae, and adults of B. carambolae in soil applications. The mortality of larvae and pupae in sterile soil treated with Metarhizium anisopliae was 70 %. In addition, 100 % of the adults that emerged from this substrate died up to five days later. This isolate caused the mortality of larvae and pupae in non-sterile soil, but its effect was more evident after adult emergence (70 % mortality up to ten days after emergence). Metarhizium robertsii was effective in killing larvae and pupae of the pest,...
Tipo: Artigo de periódico Palavras-chave: Controle Biológico; Praga de Planta; Mosca das Frutas; Fruit flies; Entomopathogenic fungi.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1109863
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AMAZONIAN ISOLATES OF Metarhizium ARE EFFECTIVE FOR KILLING Bactrocera carambolae (DIPTERA: TEPHRITIDAE) Acta biol.Colomb.
BRITO,Bruna David; LIMA,Adilson Lopes; CRUZ,Kennedy Rodrigues; BARIANI,Adriana; JESUS-BARROS,Cristiane Ramos; PEREIRA,José Francisco; ADAIME,Ricardo.
ABSTRACT Bactrocera carambolae Drew & Hancock is a quarantine pest present in Brazil and is the main phytosanitary barrier for the export of fresh fruits from that country. In this work, we evaluated the effect of Amazonian isolates of Metarhizium on kill larvae, pupae, and adults of B. carambolae in soil applications. The mortality of larvae and pupae in sterile soil treated with Metarhizium anisopliae was 70 %. In addition, 100 % of the adults that emerged from this substrate died up to five days later. This isolate caused the mortality of larvae and pupae in non-sterile soil, but its effect was more evident after adult emergence (70 % mortality up to ten days after emergence). Metarhizium robertsii was effective in killing larvae and pupae of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Carambola fruit fly; Entomopathogenic fungi; Fruit flies.
Ano: 2019 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000100118
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Beauveria Bassiana Applied to Broiler Chicken Houses as Biocontrol of Alphitobius Diaperinus Panzer (Coleoptera: Tenebrionidae), an Avian Pathogens Vector Rev. Bras. Ciênc. Avic.
Alves,LFA; Oliveira,DGP; Lambkin,T; Bonini,AK; Alves,V; Pinto,FGS; Scur,MC.
ABSTRACT Beauveria bassiana is naturally found in broiler chicken houses, and has been shown to be active against the lesser mealworm, Alphitobius diaperinus. The effectiveness of soil application of B. bassiana in emulsifiable oil-type formulation (Unioeste 4 isolate) to control the lesser mealworm in commercial poultry house was evaluated. The fungus was applied to the dirt floor of poultry house at 4.2 × 109 conidia/m2 (treated aviary) and the insect population was assessed before and 96, 146 and 216 days after application (DAA). In the control aviary, no treatment was performed to control those insects. Molecular techniques were used to confirm the presence of the fungus in dead A. diaperinus. Significant treatment effects were observed, with 56% and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal production; Biological control; Entomopathogenic fungi.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400459
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Cebos para el econtrol de la cucaracha alemana Blatella germanica (Dictyoptera: Blattellidae) formulados con hongos entomopatógenos y ácido bórico. Colegio de Postgraduados
Hernández Ramírez, Gabriela.
Blattella germanica (Dictyoptera: Blattellidae) es la especie más importante de las cucarachas sinantrópicas en el mundo. En este trabajo se evaluó la patogenicidad de aislamientos de Metarhizium anisopliae, M. pingshaense y Beauveria bassiana en ninfas y adultos de B. germanica. Para ofrecer una alternativa al uso de insecticidas en el manejo de B germanica, se desarrolló una formulación de cebo en polvo y pellet. Se determinó la preferencia de adultos de esta cucaracha, a diferentes dosis de azúcar glas (0.125, 0.25, 0.5, 0.75M) como fagoestimulante y se evaluó la compatibilidad del ácido bórico con los hongos M anisopliae y B bassiana en estos cebos. Finalmente, se evaluó vía oral, la virulencia de cebos formulados con azúcar glas 0.5 M, ácido bórico...
Palavras-chave: Cucaracha alemana; Hongo entomopatógeno; Plagas urbanas; Virulencia; German cockroach; Entomopathogenic fungi; Pathogenicity; Urban pest; Entomología y Acarología; Doctorado.
Ano: 2013 URL: http://hdl.handle.net/10521/1867
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Combination of the fungus Beauveria bassiana and pheromone in an attract-and-kill strategy against the banana weevil, Cosmopolites sordidus. Repositório Alice
LOPES, R. B.; LAUMANN, R. A.; MOORE, D.; OLIVEIRA, M. W. M.; FARIA, M..
Tipo: Separatas Palavras-chave: Cosmolure; Delivery system; Fat pellet; Hydrogenated oil; Formulation; Microbial control; Coleoptera; Curculionidae; Entomopathogenic fungi; Mycoinsecticides; Shelf life.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1006081
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Como o uso do controle biológico tem crescido na cultura do algodoeiro, que fatores limitam sua adoção e como a pesquisa tem trabalhado para aumentar a gama de ferramentas e tecnologias disponíveis ao produtor. Repositório Alice
MIRANDA, J. E.; SILVA, C. S. B. da.
Na cultura do algodoeiro, embora diversas estratégias que compõem o manejo integrado para conter a população de pragas o controle mais adotado ainda é o químico. Mas a utilização constante deste método de controle tem levado à seleção de organismos-praga resistentes, além de onerar o custo de produção. O controle biológico é definido como o uso de agentes predadores parasitoides e/ou entomopatógenos com o objetivo de reduzir as populações de pragas. Já se observa uma tendência de aumento do uso de agentes de controle biológico ou bioinsumos no Brasil.
Tipo: Artigo de periódico Palavras-chave: Bicudo do algodoeiro; Lagartas; Produtos biológicos; Algodão; Spodoptera Frugiperda; Praga; Controle Biológico; Bacillus Thuringiensis; Pulgão; Fungo Para Controle Biológico; Baculovirus; Bactéria; Percevejo; Ácaro; Biological control; Cotton; Entomopathogenic fungi.
Ano: 2022 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1146310
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Compatibility of conventional agrochemicals used in rice crops with the entomopathogenic fungus Metarhizium anisopliae Scientia Agricola
Silva,Rodrigo Alves da; Quintela,Eliane Dias; Mascarin,Gabriel Moura; Barrigossi,José Alexandre Freitas; Lião,Luciano Moraes.
The toxicological impact of chemical pesticides on fungal entomopathogens and their use in tank-mixing can be directly measured through in vitro compatibility tests. This study reports the in vitro toxicity of eight insecticides, four fungicides and five herbicides in the conidial germination, vegetative growth and conidiation of Metarhizium anisopliae (strain CG 168). A conidial suspension containing the pesticide at recommended field dosage was subjected to constant agitation in a rotary shaker for 3h to simulate a tank mixing. Then, aliquots of each suspension were used to determine conidial germination, vegetative growth and conidiation on potato dextrose agar (PDA). The fungicides difenoconazole (69 mL ha-1), propiconazole (75 mL ha-1),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pesticide compatibility; Conidiation; Entomopathogenic fungi; Germination; Vegetative growth.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000300003
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Consortium of five fungal isolates conditioning root growth and arbuscular mycorrhiza in soybean, corn, and sugarcane Anais da ABC (AABC)
FARIAS,CHRISTYAN P.; CARVALHO,RAFAEL C. DE; RESENDE,FELIPE M.L.; AZEVEDO,LUCAS C.B..
Abstract Plant growth and arbuscular mycorrhizal colonization were studied in sugarcane, corn and soybean by applying five plant growth promoting fungi: Beauveria bassiana, Metarhizium anisopliae, Pochonia chlamydosporia, Purpureocillium lilacinum, and Trichoderma asperella. Sugarcane, corn and soybean were grown in pots under two treatments: (1) inoculation with the fungal consortium and (2) control without inoculation. In the inoculated treatment, fungal spore suspension were applied to the seeds and shoots were sprayed every 28 days. Means were analyzed by analysis of variance and Tukey’s test at 5% probability level. The experiment was arranged in a completely randomized design, with six replications. Fungi consortium mediate root growth in soybean and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant growth promoting fungi; Entomopathogenic fungi; Arbuscular mycorrhizal; Phytohormones.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703649
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Controle biológico de plantas daninhas com fungos fitopatogênicos. Repositório Alice
VIEIRA, B. S.; BARRETO, R. W.; NECHET, K. de L..
Tipo: Separatas Palavras-chave: Erva Daninha; Controle Biológico; Fungo Para Controle Biológico; Weeds; Biological control; Entomopathogenic fungi.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1104834
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Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa BABT
Mascarin,Gabriel Moura; Alves,Sérgio Batista; Lopes,Rogério Biaggioni.
This work investigated the production of the fungi Isaria fumosorosea and Isaria farinosa in biphasic fermentation using agro-industrial products and residues. Combinations of natural liquid substrates, alternative to the complete medium and potato dextrose medium, were evaluated. The best liquid media were sugarcane molasses + rice broth, rice broth + yeast and sugarcane molasses + yeast + rice broth, which resulted in the highest viable propagule concentration. The molasses + rice broth medium was selected for the next phase of the study in which the production of both fungal isolates was evaluated in solid grain substrates. In solid-state fermentation, the best conidia production was achieved with the soybean meal and broken corn for I. farinosa, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biphasic fermentation; Entomopathogenic fungi; Isaria fumosorosea; Isaria farinosa; Agro-industrial products.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400002
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Detection of Metarhizium anisopliae var. anisopliae within infected sugarcane borer Diatraea saccharalis (Lepidoptera, Pyralidae) using specific primers Genet. Mol. Biol.
Destéfano,Ricardo Henri Rodrigues; Destéfano,Suzete A. Lanza; Messias,Claudio Luiz.
In order to construct specific primers for the detection and identification of the entomopathogenic fungus Metarhizium within infected sugarcane borer (Diatraea saccharalis) larvae we analyzed the ITS1 -5.8S- ITS2 rDNA regions of strains and varieties of M. anisopliae, M. album and M. flavoviride. The PCR amplification of these regions yielded a unique fragment of approximately 540 bp for M. anisopliae variety anisopliae strains E9, B/Vi and C (isolated in Brazil), 600 pb for M. a. anisopliae strain 14 (isolated in Australia), 650 bp for the M. album and 600 bp for M. flavoviride strains. The PCR products were digested with different restriction endonucleases (Afa I, Alu I, Dde I, Hae III, Hpa II and Sau 3A) and the PCR-RFLP profiles showed clear...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metarhizium anisopliae; Entomopathogenic fungi; PCR-RFLP; ITS region.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200020
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Differential susceptibility of adults and nymphs of Blattella germanica (L.) (Blattodea: Blattellidae) to infection by Metarhizium anisopliae and assessment of delivery strategies Neotropical Entomology
Lopes,RB; Alves,SB.
Microbial insecticides for cockroach control, such as those containing entomopathogenic fungi, may be an alternative to reduce contamination by chemicals in housing and food storage environments. Virulence of isolate ESALQ1037 belonging to the Metarhizium anisopliae complex against nymphs and adults of Blattella germanica (L.), and its infectivity following exposure of insects to a contaminated surface or to M. anisopliae-bait were determined under laboratory conditions. Estimated LD50 15 d following topical inoculation was 2.69 x 10(5) conidia per adult, whereas for nymphs the maximum mortality was lower than 50%. Baits amended with M. anisopliae conidia had no repellent effect on targets; adult mortality was inferior to 25%, and nymphs were not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: German cockroach; Microbial control; Entomopathogenic fungi.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000300010
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Effect of entomopathogens on Africanized Apis mellifera L. (Hymenoptera: Apidae) Rev. Bras. entomol.
Potrich,Michele; Silva,Rita T.L. da; Maia,Fabiana M.C.; Lozano,Everton R.; Rossi,Robson M.; Colombo,Fernanda C.; Tedesco,Flavia G.; Gouvea,Alfredo de.
ABSTRACT This study aimed to evaluate the effect of commercially used entomopathogens on Africanized Apis mellifera L. (Hymenoptera: Apidae). Four bioassays were performed: 1) pulverized entomopathogens on A. mellifera; 2) entomopathogens sprayed on a smooth surface; 3) entomopathogens sprayed on soy leaves; and 4) entomopathogens mixed with candy paste (sugar syrup). Five treatments were prepared: sterile distilled water (control), distilled water sterilized with Tween® 80 (0.01%), and the commercial entomopathogens Metarhizium anisopliae E9 (1.0 × 109 conidia mL−1), Beauveria bassiana PL63 (1.0 × 108 conidia mL−1) and Bacillus thuringiensis var. kurstaki HD-1 (3.0 × 108 spores mL−1). Each treatment consisted of five repetitions, with 20 workers per...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bayesian statistics; Entomopathogenic fungi; Entomopathogenic bacteria; Honeybee; Selectivity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262018000100023
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Effects of the temperature and storage on formulations with Mycelia of Beauveria bassiana (Bals.) Vuill and Metarhizium anisopliae (Metschn.) Sorok BABT
Marques,Edmilson J.; Alves,Sérgio B.; Marques,Irene M. R..
This research deals with preparation of experimental formulations with mycelia of Beauveria bassiana and Metarhizium anisopliae. The formulations were prepared with sodium alginate and pre-gelatinized corn-starch. They were kept at 25, 30 and 35±1ºC. Pre-gelatinized corn starch was more suitable that the sodium alginate for the preparations of formulations with mycelia of B. bassiana and M. anisopliae.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Formulation; Entomopathogenic fungi; Microbial Control.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000200004
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Eficiência de Beauveria bassiana Vuill. e Isaria sp. para o controle de Thaumastocoris peregrinus Carpintero & Dellapé (Hemiptera: Thaumastocoridae). Repositório Alice
LORENCETTI, G. A. T.; POTRICH, M.; MAZARO, S. M.; LOZANO, E. R.; BARBOSA, L. R.; MENEZES, M. J. S.; GONÇALVES, T. E..
A busca por informações para o controle de Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae) em Eucalyptus concentra-se no uso de técnicas de controle biológico, entre elas, o uso de fungos entomopatogênicos. Assim, o objetivo deste trabalho, foi avaliar a patogenicidade de isolados de Beauveria bassiana e Isaria sp. sobre Thaumastocoris peregrinus em condições de laboratório. Para isto, foram testados quatro isolados de Beauveria bassiana e um isolado de Isaria sp. na concentração de 1,0 × 108 conídios.mL-1. A solução foi aplicada sobre folhas de Eucalyptus camaldulensis, fornecidas como substrato de alimentação aos insetos, e mantidas em frascos de vidro vedados com filme plástico em câmara climatizada (26 ± 2°C, 14 h de fotofase e U.R. de 70 ±...
Tipo: Artigo de periódico Palavras-chave: Thaumastocoris peregrinus; Percevejo bronzeado; Fungo entomopatogênico; Bronze bug; Eucalipto; Controle biológico; Fungo para controle biológico; Eucalyptus; Biological control; Entomopathogenic fungi.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1090404
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Entomopathogenic fungi as the microbial frontline against the alien Eucalyptus pest Gonipterus platensis in Brazil. Repositório Alice
JORDAN, C.; SANTOS, P. L. dos; OLIVEIRA, L. R. dos S.; DOMINGUES, M. M.; GÊA, B. C. C.; RIBEIRO, M. F.; MASCARIN, G. M.; WILCKEN, C. F..
Abstract: The eucalyptus snout beetle (ESB), Gonipterus platensis, is endemic to Australia but has become a major invasive, destructive pest of Brazilian eucalyptus plantations. Efforts to develop insecticides based on entomopathogenic fungi against ESB are limited by the lack of known virulent strains. We therefore explored the virulence of indigenous Brazilian strains of major entomopathogenic fungi?Beauveria spp. and Metarhizium anisopliae?against ESB adults. We found widely varying virulence and later capacities for conidial production on infected adult cadavers. Two strains stood out, B. bassiana IBCB-240 and M. anisopliae IBCB-364, as especially lethal for ESB adults under laboratory conditions, sporulated abundantly on infected insects, and also...
Tipo: Artigo de periódico Palavras-chave: Besouro; Praga de Planta; Eucalipto; Fungo Para Controle Biológico; Metarhizium Anisopliae; Beauveria Bassiana; Fungo Entomógeno; Curculionidae; Plant pests; Biological control agents; Entomopathogenic fungi; Eucalyptus; Pathogens.; Entomology; Fungi.
Ano: 2021 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133908
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Enzymatic comparison and mortality of Beauveria bassiana against cabbage caterpillar Pieris brassicae LINN BJM
Dhawan,Manish; Joshi,Neelam.
Abstract Beauveria bassiana, an entomopathogenic fungus, is the alternative biocontrol agent exploited against major economic crop pests. Pieris brassicae L. is an emerging pest of the Brassicaceae family. Therefore, in the present study, fungal isolates of Beauveria bassiana, viz. MTCC 2028, MTCC 4495, MTCC 6291, and NBAII-11, were evaluated for their virulence against third instar larvae of P. brassicae. Among all these fungal isolates, maximum mortality (86.66%) was recorded in B. bassiana MTCC 4495 at higher concentration of spores (109 conidia/ml), and the minimum mortality (30.00%) was recorded in B. bassiana MTCC 6291 at a lower concentration (107 conidia/ml) after ten days of treatment. The extracellular cuticle-degrading enzyme activities of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Entomopathogenic fungi; Lipase; Mortality; Protease.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300522
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Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field Trials Chilean J. Agric. Res.
Rodríguez,Marta; Gerding,Marcos; France,Andrés; Ceballos,Ricardo.
The effectiveness of the Metarhizium anisopliae (Metschinkoff) Qu-M845 isolate was evaluated in laboratory and field trials. It was previously selected for thermal resistance (at 30 and 35 ºC) and pathogenicity on Varroa destructor (Anderson and Trueman). In the laboratory, the first evaluations were carried out by spraying and increasing the concentration from zero to 10(8) conidia mL-1 on varroa adults. Lethal concentrations required for 50 and 90% mite mortality (LC50 and LC90) were 3.8 x 10(5) and 8 x 10(7) conidia mL-1, respectively (χ2 = 2.03). In the autumn field trials, three application methods (doses of 5 x 10(10) conidia per hive) were evaluated. The treatments were: a) conidia stamped on filter paper, located on every second frame...
Tipo: Journal article Palavras-chave: Biological control; Entomopathogenic fungi; Varroasis; Insect pathology.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400009
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Exploración de enemigos naturales nativos de Rhagoletis pomonella Walsh, (Díptera: Tephritidae) en tejocote Crataegus spp. en comunidades del centro de México. Colegio de Postgraduados
Muñiz Reyes, Erica.
El manejo del tefrítido Rhagoletis pomonella en zonas productoras de tejocote Crataegus spp. en México, se realiza bajo un esquema convencional basado en el uso de insecticidas químicos. Es importante considerar una transición agroecológica, que comience por la unidad productiva, para lo cual es esencial la participación de los productores agrícolas involucrados. La inclusión de la investigación social en este estudio permitió conocer la manera en la que los productores enfrentan el problema fitosanitario en este cultivo y las expectativas que tienen al respecto. Con base en lo anterior, se propuso buscar los enemigos naturales nativos con potencial de uso en el control biológico de R. pomonella que apoye la estrategia de conservación en los huertos. Se...
Palavras-chave: Moscas de la fruta; Organización campesina; Asociación de cultivos; Control biológico; Parasitoides; Hongos entomopatógenos; Patogenicidad; Fruit fly; Farmer organization; Crop association; Biological control; Parasitoids; Entomopathogenic fungi; Pathogenicity; Doctorado; Entomología y Acarología.
Ano: 2011 URL: http://hdl.handle.net/10521/530
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Exploración de enemigos naturales nativos de Rhagoletis pomonella Walsh, (Díptera: Tephritidae) en tejocote Crataegus spp. en comunidades del centro de México. Colegio de Postgraduados
Muñiz Reyes, Erica.
El manejo del tefrítido Rhagoletis pomonella en zonas productoras de tejocote Crataegus spp. en México, se realiza bajo un esquema convencional basado en el uso de insecticidas químicos. Es importante considerar una transición agroecológica, que comience por la unidad productiva, para lo cual es esencial la participación de los productores agrícolas involucrados. La inclusión de la investigación social en este estudio permitió conocer la manera en la que los productores enfrentan el problema fitosanitario en este cultivo y las expectativas que tienen al respecto. Con base en lo anterior, se propuso buscar los enemigos naturales nativos con potencial de uso en el control biológico de R. pomonella que apoye la estrategia de conservación en los huertos. Se...
Palavras-chave: Moscas de la fruta; Organización campesina; Asociación de cultivos; Control biológico; Parasitoides; Hongos entomopatógenos; Patogenicidad; Fruit fly; Farmer organization; Crop association; Biological control; Parasitoids; Entomopathogenic fungi; Pathogenicity; Doctorado; Entomología y Acarología.
Ano: 2011 URL: http://hdl.handle.net/10521/530
Registros recuperados: 54
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