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Biochemical characteristics of typical and atypical Staphylococcus aureus in mastitic milk and environmental samples of Brazilian dairy farms BJM
Silva,Wladimir Padilha da; Destro,Maria Teresa; Landgraf,Mariza; Franco,Bernadette D.G.M..
This study reports results on the biochemical characteristics of 274 Staphylococcus aureus strains isolated from mastitic milk, hands of milkmen and milking machines. Features included colony morphology on Baird Parker agar (BPA), catalase, coagulase and thermonuclease tests. API-Staph (bioMérieux, France) was used as reference identification system. 39.4% of the colonies on BPA identified as S.aureus were atypical. They were more frequent in milk samples (94.4%). All S.aureus strains were coagulase positive but intensity of the test varied according to the source: environmental strains were 3+ or 4+, while 17.1% of strains isolated from milk presented only 1+ or 2+ results. The majority of atypical S.aureus strains presented a 4+ coagulase result. 2.2% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Atypical S.aureus; Coagulase; Thermonuclease; API-Staph; Baird-Parker agar; Milk; Mastitis; Environmental samples.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200008
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Biotechnology of polyketides: new breath of life for the novel antibiotic genetic pathways discovery through metagenomics BJM
Gomes,Elisângela Soares; Schuch,Viviane; Lemos,Eliana Gertrudes de Macedo.
The discovery of secondary metabolites produced by microorganisms (e.g., penicillin in 1928) and the beginning of their industrial application (1940) opened new doors to what has been the main medication source for the treatment of infectious diseases and tumors. In fact, approximately 80 years after the discovery of the first antibiotic compound, and despite all of the warnings about the failure of the "goose that laid the golden egg," the potential of this wealth is still inexorable: simply adjust the focus from "micro" to "nano", that means changing the look from microorganisms to nanograms of DNA. Then, the search for new drugs, driven by genetic engineering combined with metagenomic strategies, shows us a way to bypass the barriers imposed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Environmental samples; Pharmacology; PKSs; New drugs.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400002
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