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Acid phosphatase activity and leaf phosphorus content in soybean cultivars Scientia Agricola
Raposo,Roberto Wagner Cavalcanti; Muraoka,Takashi; Basso,Luiz Carlos; Lavres Jr.,José; Franzini,Vinicius Ide.
The phosphate fertilization represents the most costly fraction of soybean crop production. Efficient soybean cultivars for P absorption and utilization in soils of medium available P are highly desirable and might contribute for increasing crop production potential. Thirty two soybean [Glycine max (L.) Merr.] cultivars recommended for 'Cerrado' and differing in growth cycle (early, semi-early, semi-late, and late) were grown in a dystrophic Typic Haplustox Cerrado soil to evaluate the acid phosphatase activity, P content in the diagnostic leaf, and shoot biomass. There were differences among the soybean cultivars within all maturation groups in acid phosphatase activity and shoot biomass. The diagnostic-leaf P-content showed significant differences on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzymatic activity; Diagnostic-leaf; Maturation group.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000400014
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Adaptive features of a bacteriolytic enzyme from the style of the mussel Choromytilus meridionalis in response to environmental fluctuations. ArchiMer
Seiderer, L; Robb, F.
The extracellular digestive apparatus of bivalve molluscs consists of a crystalline style containing diverse degradative enzymes which are released during rotation and dissolution of the style. This paper reviews the occurrence of a temperature-regulated bacteriolytic enzyme in the black mussel Choromytilus meridionalis , which is an inhabitant of coastal reefs on the south and south west coast of South Africa. This agent is unable to lyse Micrococcus luteus or Escherichia coli , both of which are susceptible to lysis by a true lysozyme. It appears to have a restricted spectrum of activity which includes both free-living and kelp-associated gram-negative bacteria. In addition, the level of activity varies dramatically according to the environmental water...
Tipo: Text Palavras-chave: Choromytilus meridionalis; Bivalvia; Digestive system; Bacteria; Upwelling; Environmental effects; Adaptations; Bacteriocides; Marine molluscs; Enzymatic activity.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-991.pdf
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Antioxidant enzymes and antioxidant activity in two soursop selections (Annona muricata L.) from Nayarit,Mexico stored at 15 °C Rev. Bras. Frutic.
Balois-Morales,Rosendo; Jiménez-Zurita,José Orlando; Alia-Tejacal,Irán; López-Guzmán,Graciela Guadalupe; Palomino-Hermosillo,Yolotzin Apatzingán; Sánchez-Herrera,Leticia Mónica.
Abstract The changes in concentration of vitamin C, enzymatic and antioxidant activity during the ripening of two soursop selections (G1 and G2) at room temperature (22 ºC) and refrigeration (15 ºC) with an HR 85% were evaluated. The content of soluble protein, the activity of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), the concentration of vitamin C, as well as the antioxidant activity was evaluated by DPPH, ABTS and FRAP methods. The initial soluble protein concentration of the G1 and G2 selections diminished at 22 and 15 °C during ripening. Fruits stored at 22 °C showed the highest CAT activity. The maximum activity of SOD was recorded on the sixth and fourth day in fruits stored at 22 and 15 ºC, respectively. Fruits stored at 22 °C...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Annona muricata; Ripening; Enzymatic activity; Antioxidant activity; Refrigeration.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000100303
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Biodegradability kraft paper coated with films emulsified chitosan and palmitic acid. Repositório Alice
REIS, A. B.; YOSHIDA, C. M. P.; VILELA, E. S. D.; NASCIMENTO, R. dos S.; MELO, I. S. de; FRANCO, T. T..
Abstract: The use of natural polymers is an alternative to polymers derived from petroleum that may reduce industrial waste and contribute to the environmental protection. Following the same reasoning, the biodegradability of paper packaging is already known for it being a product originated from cellulosic materials (cellulosic fibers). The aim of this work was to assess the process of degradation from the junction of two different materials; biopolymer and sheets of paper. This paper proposes the study of the biodegradability of chitosan film-coated Kraft paper sheets and emulsified chitosan film-coated Kraft paper sheets added with palmitic acid, both in comparison with uncoated Kraft paper sheets. The biodegradability study was conducted based on...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Biopolymer; Kraft paper; Microbial biomass carbon; Soil respiration; Biofilm formation; Enzymatic activity; Biodegradação; Papel; Biofilme; Biodegradation; Chitosan.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/967553
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Biological Activity in a Degraded Alfisol Amended with Sewage Sludge and Cropped with Yellow Serradela (Ornithopus compressus L.) Chilean J. Agric. Res.
Celis H,José; Machuca H,Angela; Sandoval E,Marco; Morales C,Patricia.
There are few studies about the impact of sewage sludge on the biological properties in Alfisols of the Chilean Coastal Range drylands. Hence, the objective of this study was to evaluate its effect on the microbial respiration and enzymatic activities of a degraded Alfisol located in the Bío Bío Region (Chile) that was cropped with yellow serradela (Ornithopus compressus L.). Sludge was added to the soil at rates of 15, 30, and 60 t ha-1; he following treatments were defined: L15-P = 15 t ha-1 sludge + O. compressus; L30-P = 30 t ha-1 sludge + O. compressus; L60-P = 60 t ha-1 sludge + O. compressus; L15 = 15 t ha-1 sludge; L30 = 30 t ha-1 sludge; L60 = 60 t ha-1 sludge; CP = non-amended soil, cropped; and C = non-amended soil, no crop. Soil microorganism...
Tipo: Journal article Palavras-chave: Biosolids; Respiration; Enzymatic activity; Degraded soils; Remediation.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100020
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Changes in enzymes, phenolic compounds, tannins, and vitamin C in various stages of jambolan (Syzygium cumini Lamark) development Ciênc. Tecnol. Aliment.
Brandão,Taís Silva de Oliveira; Sena,Amanda Reges de; Teshima,Elisa; David,Jorge Maurício; Assis,Sandra Aparecida.
The physiological state of a fruit is closely related to ripening and climatic conditions during the growing period when the fruit undergo changes in color, texture, and flavor. The ripening of the fruit can involve a complex series of biochemical reactions with alteration in enzymes activities, phenols, tannins, and ascorbic acid. The activity of enzymes (carboximethylcellulase, polygalacturonase, and pectinlyase), the total concentration of phenolic compounds, condensed tannins, and vitamin C in five stages of maturation were studied. Significant changes were observed between the maturity stages. The phenolic compounds were higher at green stage (705.01 ± 7.41); tannins were higher at green/purple stage (699.45 ± 0.22). The results showed that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Syzygium cumini Lam.; Maturity; Total phenolic compounds; Tannins; Vitamin C; Enzymatic activity.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000400004
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Characterization of a leucine aminopeptidase from Toxoplasma gondii OAK
Jia, Honglin; Nishikawa, Yoshifumi; Luo, Yuzi; Yamagishi, Junya; Sugimoto, Chihiro; Xuan, Xuenan.
The M17 family leucine aminopeptidase (LAP) hydrolyze amino acids from the N-terminus of peptides. Many LAPs from parasitic protozoa including Plasmmodium, Trypanosoma and Leishmania, have been intensely investigated because of their crucial roles in parasite biology. In this study, a functional recombinant Toxoplasma gondii LAP (rTgLAP) was expressed in Escherichia coli and its enzymatic activity against synthetic substrates for aminopeptidase, as well as the cellular localization was determined. Our results indicated that TgLAP is a functional aminopeptidase in the cytoplasma of T. gondii.
Palavras-chave: Toxoplasma gondii; Leucine aminopeptidase; Enzymatic activity.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2822
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Chemical and Biochemical Properties of Oxisols after Sewage Sludge Application for 16 Years Rev. Bras. Ciênc. Solo
Yada,Marcela Midori; Melo,Wanderley José de; Mingotte,Fábio Luiz Checchio; Melo,Valéria Peruca de; Melo,Gabriel Maurício Peruca de.
ABSTRACT The large production of sewage sludge (SS), especially in large urban centers, has led to the suggestion of using this waste as fertilizer in agriculture. The economic viability of this action is great and contributes to improve the environment by cycling the nutrients present in this waste, including high contents of organic matter and plant nutrients. This study evaluated the chemical and biochemical properties of Dystrophic and EutroferricLatossolos Vermelhos (Oxisols) under corn and after SS application at different rates for 16 years. The field experiment was carried out in Jaboticabal, São Paulo State, Brazil, using a randomized block design with four treatments and five replications. Treatments consisted of control - T1 (mineral...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soil fertility; Enzymatic activity; Soil basal respiration; Microbial biomass; Urban waste.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000501303
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Cloning and initial characterization of cellulolytic enzymes selected in a metagenomic approach. Repositório Alice
FAHEEM, M.; VIDAL, J. F. D.; FERNANDES, J. P. C.; OLIVEIRA. G. M.; CAROLINE, C. F.; SOUTO, B. de M.; PARACHIN, N. S.; QUIRINO, B. F.; BARBOSA, J. A. R. G..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Biofuel; Metagenome; Cellulose degradation; Enzymatic activity; Protein structure.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/961702
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Cloning, expression and characterization of alcohol dehydrogenases in the silkworm Bombyx mori Genet. Mol. Biol.
Wang,Nan; Shi,Haifeng; Yao,Qin; Zhou,Yang; Kang,Lequn; Chen,Huiqin; Chen,Keping.
Alcohol dehydrogenases (ADH) are a class of enzymes that catalyze the reversible oxidation of alcohols to corresponding aldehydes or ketones, by using either nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP), as coenzymes. In this study, a short-chain ADH gene was identified in Bombyx mori by 5'-RACE PCR. This is the first time the coding region of BmADH has been cloned, expressed, purified and then characterized. The cDNA fragment encoding the BmADH protein was amplified from a pool of silkworm cDNAs by PCR, and then cloned into E. coli expression vector pET-30a(+). The recombinant His-tagged BmADH protein was expressed in E. coli BL21 (DE3), and then purified by metal chelating affinity chromatography. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5'-RACE PCRADH; Enzymatic activity; Recombinant protein.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200013
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Comparative analysis of enzymatic activity of tannase in non-conventional yeasts to produce ellagic acid Ciênc. Tecnol. Aliment.
Márquez-López,Anahí; Ramírez-Conejo,Juan David; Chávez-Parga,Ma. Del Carmen; Flores,Dora Cecilia Valencia; Jaramillo,Miguel Angel Zamudio; Rodríguez,Horacio González; González-Hernández,Juan Carlos.
Abstract Non-conventional yeasts represent an alternative for microorganisms that are capable to produce the enzyme Tannase (EC 3.1.1.20), which has many applications is the food and pharmaceutical industry as it stands out for its ability to produce ellagic acid out of the enzymatic hydrolysis of ellagitannins. Ellagic acid has been studied in the medical area for its action against various diseases, as is the case of cancer or heart problems. The investigation was conducted to evaluate seven non-conventional yeasts - Debaryomyces hansenii PYC ISA 1510, Debaryomyces hansenii PYC 2968, Candida parapsilosis, Candida utilis, Pichia pastoris, Pichia kluyveri, Issatchenkia terricola - as producers of the enzyme tannase. This was done in mediums of fermentation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Non-conventional yeasts; Enzymatic activity; Tannase; Ellagic acid; Hydrolysis.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005031111
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Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation BABT
Leite,Nadjma Souza; Lima,Aloísio André Bomfim de; Santana,José Carlos Curvelo; Lopes,Francisco Luiz Gumes; Lédo,Ana da Silva; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de.
This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzymatic activity; Bromelain; Pérola pineapple; Imperial pineapple; Production in vitro.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500002
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Different feeding habits influence the activity of digestive enzymes in freshwater fish Ciência Rural
Gioda,Carolina Rosa; Pretto,Alexandra; Freitas,Carine de Souza; Leitemperger,Jossiele; Loro,Vania Lucia; Lazzari,Rafael; Lissner,Leandro Ademar; Baldisserotto,Bernardo; Salbego,Joseânia.
ABSTRACT: The aim of this study was to verify the activity of some digestive enzymes in four fish species with different feeding habits. Knowledge of these enzymatic activities can help us to understand the species' digestive processes. The species chosen for this study were Ctenopharyngodon idella (herbivore), Rhamdia quelen (omnivore), Leporinus obtusidens (omnivore) and Hoplias malabaricus (carnivore). The digestive tract of these species was divided into four portions to estimate enzymatic activity: stomach, anterior, mid and posterior intestine. Ctenopharyngodon idella had the highest amylase and maltase activities in all portions of the gut, followed by L. obtusidens . The highest trypsin activity was observed in all gut portions of H. malabaricus,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Freshwater fishes; Feeding habits; Enzymatic activity; Amylase; Alkaline protease; Maltase; Trypsin.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300406
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Dynamique et enzymologie de la digestion chez l'huitre Crassostrea gigas ArchiMer
Boucaud, C; Lebesnerais, C; Lubet, P; Lihrmann, I.
Feeding experiments carried out on Crassostrea gigas allowed the authors to set up the stages and the timing of the movement of food throughout the digestive tract. Various activities involved in digestion (glycosidases, esterases, and peptidases) were investigated histochemically in the digestive tract in order to localize the sites of absorption and the sites of protein and carbohydrate digestion. An explicative model of the different stages of digestion is proposed.
Tipo: Text Palavras-chave: Crassostrea gigas; Bivalvia; Cytology; Histochemistry; Histology; Digestive system; Enzymatic activity; Digestive glands; Digestion; Enzymes; Transit digestif; Digestion; Huitre; Crassostrea gigas.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1179.pdf
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Effect of broiler breeder age on pancreas enzymes activity and digestive tract weight of embryos and chicks Rev. Bras. Ciênc. Avic.
Maiorka,A; Santin,E; Silva,AVF; Routman,KS; Pizauro Jr.,JM; Macari,M.
A study was carried out to evaluate the effect of broiler breeder age on the development of the digestive tract of embryos and chicks. Fertilized eggs Cobb from 30 and 60 week-old broiler breeder was utilized in this experiment. The results showed that eggs from older (60 weeks of age) broiler breeders were heavier (p = 0.001) than those from younger (30 weeks of age) broiler breeder. In addition, older broiler breeder had larger (p = 0.001) embryos showing a higher yolk sac (p = 0.001) and higher gastrointestinal tract relative weight (p = 0.007) than those from younger broiler breeder. The activities of pancreatic lipase and trypsin enzymes were also higher in embryos from older broiler breeder than those from younger broiler breeder (p = 0.001 and p =...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler breeder age; Embryo; Enzymatic activity; Gastrointestinal tract.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000100003
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Effect of ph and temperature on the activity of phytase products used in broiler nutrition Rev. Bras. Ciênc. Avic.
Naves,L de P; Corrêa,AD; Bertechini,AG; Gomide,EM; Santos,CD dos.
The activity of three commercial microbial phytase (Aspergillus oryzae, A. niger, and Saccharomyces cerevisae) products used in broiler nutrition was determined at different pH (2.0 to 9.0) and temperature (20 to 90°C) values. Enzymatic activity was determined according to the reaction of the phytase with its substrate (sodium phytate), in four replicates, and was expressed in units of phytase activity (FTU). A. oryzae phytase exhibited optimal activity at pH 4.0 and 40°C, but its absolute activity was the lowest of the three phytases evaluated. A. niger phytase exhibited maximal activity close to pH 5.0 and 45ºC, whereas S. cerevisae phytase presented its highest activity at pH close to 4.5 and temperatures ranging between 50 and 60°C. It was concluded...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzymatic activity; Enzyme; Phytate; Phytic acid.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000300004
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Enzymatic polymorphism study as help for constitution of initial broodstock for a new cultivated finfish species. ArchiMer
Renno, J; Luquet, P.
Leporinus friderici is a South American promising species for fish culture. In French Guiana the "morphological species", L. friderici, L. granti, L. lebaili and L. affinis steyermarki are found. Their identification is sometimes difficult on usual criterion. To constitute an initial broodstock of L. friderici from local wild populations, the genetic structure of these species was studied. Fifteen enzyme systems, representing twenty loci, were screened.
Tipo: Text Palavras-chave: French Guiana; Leporinus; Leporinus granti; Leporinus friderici; Anostomidae; Pisces; Embryos; Mortality; Genetics; Freshwater fish; Rearing; Breeding; Biopolymorphism; Brood stocks; Enzymatic activity.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1441.pdf
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Enzymatic regulation of photosynthetic and light-independent carbon fixation in Laminaria setchellii (Phaeophyta), Ulva lactuca (Chlorophyta) and Iridaea cordata (Rhodophyta) RChHN
CABELLO-PASINI,ALEJANDRO; ALBERTE,RANDALL S..
Carbon is acquired through photosynthetic and non-photosynthetic processes in marine algae. However, little is known about the biochemical regulation of these metabolic pathways along the thallus of seaweeds. Consequently, the objective of this study was to assess the distribution of in vivo carboxylation pathways and to relate them to the in vitro activity of ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO), phosphoenolpyruvate carboxykinase (PEPCK), and phosphoenolpyruvate carboxylase (PEPC) in the Phaeophyte Laminaria setchellii, the Chlorophyte Ulva lactuca, and the Rhodophyte Iridaea cordata. Chlorophyll-a levels did not vary in U. lactuca and I. cordata. However, pigment levels were significantly lower in the meristematic region of L....
Tipo: Journal article Palavras-chave: Enzymatic activity; Chlorophyll; Light-independent carbon fixation; Photosynthesis.
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000200002
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Etude de la fraction azotee soluble de l'anchois sale en cours de maturation ArchiMer
Durand, Patrick.
Different parameters have been followed regularly at various times of anchovy (Engraulis encrasicholus ) ripening. Concentration in NaCl and water content as well as values of water activity and pH are constant and remain quite stable during the entire study period. The relatively acid pH value and the quite low measured water activity level created good conditions for preventing microbial putrefaction at room temperature. The free amino acids and non proteinaceous nitrogen increase indicated the modifications occurring in muscular proteins. These changes resulted in an important proteolytic activity modulated by the medium conditions, especially; pH, temperature and ionic strength. The enzymatic activity slowed down regularly with time but was not...
Tipo: Text Palavras-chave: Enzymatic activity; Nitrogen compounds; PH; Biochemical composition; Cured products.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/1981/publication-1889.pdf
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Etude histoensymologique de la digestion chez Ruditapes philippinarum ArchiMer
Hily, A.
Examinations of alimentary tract and digestive tubules after feeding give evidence of continuous digestive process in juveniles of Ruditapes philippinarum . Histoenzymologic study of digestive tract suggests important intracellular enzymatic activities in stomacal epithelium, digestive gland and intestinal cells. Main part of intracellular digestion occurred in digestive cells by lysosyme associated with vacuolar system.
Tipo: Text Palavras-chave: Ruditapes philippinarum; Bivalvia; Digestive system; Enzymatic activity; Histology; Digestive glands; Digestion; Enzymes digestives; Digestion; Bivalve.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1180.pdf
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