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Assessment of sugarcane trash for agronomic and energy purposes in Brazil Scientia Agricola
Franco,Henrique Coutinho Junqueira; Pimenta,Maria Teresa Borges; Carvalho,João Luís Nunes; Magalhães,Paulo Sérgio Graziano; Rossell,Carlos Eduardo Vaz; Braunbeck,Oscar Antonio; Vitti,André Cesar; Kölln,Oriel Tiago; Rossi Neto,João.
Due to new possibilities for using sugarcane (Saccharum spp.) trash for electricity generation, and the production of 2nd generation ethanol and others chemicals, the interest for its recovery has increased. However, the question of how much trash can be removed from sugarcane field still needs to be clarified. This study evaluated the amount of dry matter, nutrients content, structural compounds and efficiency of the enzymatic hydrolysis of the hydrothermal pretreated materials for tops and dry leaves in samples from sugarcane varieties. Tops and dry leaves present differences in nutrients content and moisture. Therefore, the amount of trash to be collected should not be simply based on percentages, but also should take into account the different...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharum spp.; Enzymatic hydrolysis; Sugarcane crop residues.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000500004
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Bioethanol production from rice straw residues BJM
Belal,Elsayed B..
A rice straw -cellulose utilizing mold was isolated from rotted rice straw residues. The efficient rice straw degrading microorganism was identified as Trichoderma reesei. The results showed that different carbon sources in liquid culture such as rice straw, carboxymethyl cellulose, filter paper, sugar cane bagasse, cotton stalk and banana stalk induced T. reesei cellulase production whereas glucose or Potato Dextrose repressed the synthesis of cellulase. T. reesei cellulase was produced by the solid state culture on rice straw medium. The optimal pH and temperature for T. reesei cellulase production were 6 and 25 ºC, respectively. Rice straw exhibited different susceptibilities towards cellulase to their conversion to reducing sugars. The present study...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase; Trichoderma reesei; Rice straw residues; Enzymatic hydrolysis; Bioethanol.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100033
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Cassava Pulp Enzymatic Hydrolysate as a Promising Feedstock for Ethanol Production BABT
Valeriano,Ixthá Hasselmann; Marques,Gabriel Lessa Lisboa; Freitas,Suely Pereira; Couri,Sonia; Penha,Edmar das Mercês; Gonçalves,Márcia Monteiro Machado.
ABSTRACT The aim of this study was to produce bioethanol from enzymatic hydrolysates of cassava pulp, a by-product of cassava flour manufacturing, using an alcohol-tolerant Saccharomyces cerevisiae strain. First, the best operational condition of the starch hydrolysis process was determined through a complete factorial design (24), with triplicates at the central point. The independent variables evaluated were: the concentrations of α-amylase (Termamyl 2X) and glucoamylase (AMG 300L) and both liquefaction and saccharification times. The most favorable hydrolysis condition in the assay was achieved using 0.517 mL AMG.g starch-1 and 0.270 mL Termamyl.g starch-1, with liquefaction and saccharification times of 1 and 2 h, respectively. The broth obtained at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Starch waste; Enzymatic hydrolysis; Alcoholic fermentation.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100207
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Clarification of pineapple juice by microfiltration Ciênc. Tecnol. Aliment.
Carvalho,Lucia Maria Jaeger de; Silva,Carlos Alberto Bento da.
In the present work, pineapple juice was first hydrolyzed with a commercial pectinase (Ultrazym 100 G) and then clarified by microfiltration. A tubular polyethersulfone membrane with an average cut-off of 0.3 µm and a total effective filtration area of 0.05 m² was applied. The transmembrane pressures were 1.5 and 3.0 bar, respectively, and the processes was conducted at room temperature. The results showed that the pineapple juice permeate fluxes were of 57.77 L/m²/hours (1.5 bar) and 46.85 L/m²/hours (3.0 bar). Concentration polarization and possibly fouling occurred during the processes. The best clarified juice fluxes were obtained when low transmembrane pressures (1.5 bar) were applied.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fruit juice; Enzymatic hydrolysis; Membrane processes.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000300040
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Effects of hydrolysis and digestion in vitro on the activity of bovine plasma hydrolysates as inhibitors of the angiotensin I converting enzyme BABT
Gómez Sampedro,Leidy Johanna; Zapata Montoya,José Edgar.
The angiotensin I-converting enzyme (ACE) inhibiting activity of bovine plasma hydrolyzates obtained by Alcalase 2.4 L at different degrees of hydrolysis (DH) was evaluated. For the evaluation of ACE inhibition (ACEI), Hippuryl-His-Leu was used as substrate and the amount of hippuric acid liberated by non-inhibiting ACE was determined by spectrophotometry at 228 nm. The results showed that the enzymatic hydrolysis increased the ACEI activity as compared with the un-hydrolyzed plasma. The highest activity was onbtained with a DH of 6.7%. The peptide fractions with the maximum activity were isolated using ultrafiltration membranes, ion exchange chromatography and high performance liquid chromatography on reverse phase (RP-HPLC). The fraction with highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzymatic hydrolysis; Bioactive peptides; Angiotensin I-converting enzyme inhibitors; Peptide sequence.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300012
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Effects of surface proteins and lipids on molecular structure, thermal properties, and enzymatic hydrolysis of rice starch Ciênc. Tecnol. Aliment.
HU,Pan; FAN,Xiaoxu; LIN,Lingshang; WANG,Juan; ZHANG,Long; WEI,Cunxu.
Abstract Rice starches with different amylose contents were treated with sodium dodecyl sulfate (SDS) to deplete surface proteins and lipids, and the changes in molecular structure, thermal properties, and enzymatic hydrolysis were evaluated. SDS treatment did not significantly change the molecular weight distribution, crystalline structure, short-range ordered degree, and gelatinization properties of starch, but significantly altered the pasting properties and increased the swelling power of starch. The removal of surface proteins and lipids increased the enzymatic hydrolysis and in vitro digestion of starch. The influences of removing surface proteins and lipids from starch on swelling power, pasting properties, and enzymatic hydrolysis were different...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzymatic hydrolysis; Molecular structure; Rice starch; Surface proteins and lipids; Thermal properties.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000100084
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Fed batch enzymatic saccharification of food waste improves the sugar concentration in the hydrolysates and eventually the ethanol fermentation by Saccharomyces cerevisiae H058 BABT
Yan,Shoubao; Yao,Jianming; Yao,Liming; Zhi,Zhijun; Chen,Xiangsong; Wu,Jingyong.
The enzymatic hydrolysis of food waste by commercially available enzymes and the subsequent ethanol fermentation of the hydrolysates by Saccharomyces cerecisiae H058 were studied in this work. The optimum batch enzymatic conditions were found to be saccharification pH of 4.5, temperature of 55!, glucoamylase concentration of 120 u/g, α-amylase concentration of 10 u/g, solid-liquid ratio of 1: 0.75 (w/w). Fed batch hydrolysis process was started with a solid-liquid ratio of 1: 1 (w/w), with solid food waste added at time lapse of 2 h to get a final solid-liquid ratio of 1: 0.5 (w/w). After 4 h of reaction, the reducing sugar concentration reached 194.43 g/L with a enzymatic digestibility of 93.12%. Further fermentation of the batch and fed batch enzymatic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Food waste; Enzymatic hydrolysis; Hydrolysates; Fermentation; Ethanol production.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200002
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Hydrolysis of various thai agricultural biomasses using the crude enzyme from Aspergillus aculeatus iizuka FR60 isolated from soil BJM
Boonmee,Atcha.
In this study, forty-two fungi from soil were isolated and tested for their carboxymethyl cellulase (CMCase) and xylanase activities. From all isolates, the fungal isolate FR60, which was identified as Aspergillus aculeatus Iizuka, showed high activities in both CMCase and xylanase with 517 mU/mg protein and 550 mU/mg protein, respectively. The crude enzyme from A. aculeatus Iizuka FR60 could hydrolyze several agricultural residues such as corncob, and sweet sorghum leaf and stalk at comparable rates with respect to the tested commercial enzymes and with a maximum rate in rice hull hydrolysis (29 μg sugar g-1 dry weight substrate mg-1 enzyme hr-1). The highest amount of glucose was obtained from corncob by using the crude enzyme from A. aculeatus Iizuka...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agricultural wastes; Aspergillus aculeatus Iizuka; Enzymatic hydrolysis; Mean specific rate; Reducing sugars.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200005
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In vitro protein digestibility of enzymatically pre-treated bean (Phaseolus vulgaris L.) flour using commercial protease and Bacillus sp. protease Ciênc. Tecnol. Aliment.
Dias,Disney Ribeiro; Abreu,Celeste Maria Patto de; Silvestre,Marialice Pinto Coelho; Schwan,Rosane Freitas.
The common bean (Phaseolus vulgaris L.) is a staple food in the Brazilian diet and represents the major source of dietary protein and other micronutrients and minerals. Despite the considerable protein concentration in beans, the food is considered of low biological value when compared to animal proteins and other plant protein sources. To improve the availability of protein in beans, enzymatic treatments were performed in four cultivars (ON, OPNS, TAL and VC3). The approach was a completely randomized design with four replicates. We used a 4 × 3 factorial arrangement (four cultivars and three treatments: treatment 1-addition of commercial protease (Trypsin 250, Difco), treatment 2-addition of protease from Bacillus sp., and treatment 3:-control without...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bean flour; Enzymatic hydrolysis; Alkaline protease.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000100014
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Influence of hydrolysis time on protein recovery and amino acid composition of hydrolysates from Sepia officinalis viscera ArchiMer
Soufi-kechaou, Emna; Jaouen, Pascal; Ben Amar, Raja; Berge, Jean-pascal.
Enzymatic hydrolysis of cuttlefish viscera by Protamex, Pepsin, Delvolase and Multifect Neutral was investigated in a batch reactor during 6 hours with an hourly sampling. The influence of the enzyme used and the hydrolysis conditions (operating pH, temperature) was studied with regards to the extent of proteolytic degradation and to molecular weight distribution of the peptides. After 360 min of hydrolysis, cuttlefish viscera treated with Pepsin yielded significantly (p < 0.05) higher protein recoveries compared to the substrate autolysed or treated with other external proteases. However, the amino acid composition showed that this was Protamex hydrolysis which gave the highest percentage of essential amino acids. At least, according to the FPLC...
Tipo: Text Palavras-chave: Enzymatic hydrolysis; Cuttlefish Protein Hydrolysates (CPH); Cuttlefish By-Products (CBP); Essential Amino Acids (EAA); Protein recovery.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00114/22508/20191.pdf
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Potential of giant reed (Arundo donax L.) for second generation ethanol production Electron. J. Biotechnol.
Lemons e Silva,Claudia Fernanda; Artigas Schirmer,Manoel; Nobuyuki Maeda,Roberto; Araújo Barcelos,Carolina; Pereira,Nei.
Background The production of second generation ethanol from lignocellulosic biomasses that have not had their potential fully explored as feedstock is of great importance. Arundo donax is one these biomasses. It is a promising grassy plant to be used as a renewable resource for the production of fuels and chemicals, because of its fast growth rate, ability to grow in different soil types and climatic conditions. The present study evaluated its use as feedstock for the production of second generation ethanol. Results Initially its chemical characterization was carried out, and a protocol for fractioning the biomass through diluted acid pretreatment followed by alkaline pretreatment was developed, providing a solid fraction which was undergone to enzymatic...
Tipo: Journal article Palavras-chave: Acid and alkaline pretreatments; Alcohol fermentation; Cellulase; Enzymatic hydrolysis; Lignocellulose.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100003
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Production et caractérisation de fractions protéiques à partir des coproduits de la sole tropicale (Cynoglossus senegalensis) ArchiMer
Sylla, K. S. B.; Musabyemariya, B.; Berge, Jean-pascal; Seydi, Mg.
The by-products (viscera, heads, backbone and skins) of tongue sole coming from a Senegal frozen fisherie plant, were hydrolized with the Protamex® enzyme.Degrees of hydrolysis of 30 and 19% were obtained respectively with 40°C and 50°C.The results also showed that the proteins contained in these by-products are completely solubilized by the action of this protease at the end of 180 minutes of hydrolysis. The biochemical characterization showed that these hydrolysates are rich in proteins (61%) and minerals (10%). However, the hydrolysates contain peptides of lower molecular sizes 1760Da.Lastly, these hydrolysates have high nutritional value in order to set up table fowls because they have in big quantity 9 essential amino acids for the poultry feed.
Tipo: Text Palavras-chave: Co-produits; Hydrolyse enzymatique; Protéines; By-products; Enzymatic hydrolysis; Proteins.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/00015/12621/9506.pdf
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Production of plant cell wall degrading enzymes by monoculture and co-culture of Aspergillus niger and Aspergillus terreus under SSF of banana peels BJM
Rehman,Shazia; Aslam,Hina; Ahmad,Aqeel; Khan,Shakeel Ahmed; Sohail,Muhammad.
Filamentous fungi are considered to be the most important group of microorganisms for the production of plant cell wall degrading enzymes (CWDE), in solid state fermentations. In this study, two fungal strains Aspergillus niger MS23 and Aspergillus terreus MS105 were screened for plant CWDE such as amylase, pectinase, xylanase and cellulases (β-glucosidase, endoglucanase and filterpaperase) using a novel substrate, Banana Peels (BP) for SSF process. This is the first study, to the best of our knowledge, to use BP as SSF substrate for plant CWDE production by co-culture of fungal strains. The titers of pectinase were significantly improved in co-culture compared to mono-culture. Furthermore, the enzyme preparations obtained from monoculture and co-culture...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid state fermentation; Plant cell wall degrading enzymes (CWDE); Banana peels; Aspergillus strains; Enzymatic hydrolysis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400045
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Purification de la chitine par hydrolyse enzymatique à partir de coproduits de crevette Penaeus vannamei. Caractérisations des produits et optimisation du procédé ArchiMer
Le Roux, Karine.
The objective of this study was to optimize the extraction of chitin by acid proteolysis. The novelty of the method is based on the stabilization of pH by the balance between substrate composition and the acid solvent. This principle allows a simultaneous demineralization and deproteinization, the two main reactions associated with the purification of chitin. To evaluate the performance of this purification, the composition of the substrate and products was characterized. Different methods of quantification of chitin and proteins have been compared. As traditional assays were not satisfaying, a direct method of amino acids determination by gaz chromatography was selected to estimate the amount of protein. The estimate of chitin amount was based on indirect...
Tipo: Text Palavras-chave: Coproduits de crustacés; Hydrolyse enzymatique; Protéolyse; Chitine; Extraction; Cinétique; Déminéralisation; Déprotéinisation; Crustacean by-products; Enzymatic hydrolysis; Proteolysis; Chitin; Extraction; Kinetic; Demineralization; Deproteinization.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00087/19828/17470.pdf
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Recovery of valuable soluble compounds from washing waters generated during small fatty pelagic surimi processing by membrane processes ArchiMer
Dumay, Justine; Radier, Severine; Barnathan, Gilles; Berge, Jean-pascal; Jaouen, Pascal.
This work focuses on the treatment of washing waters coming from surimi manufacturing using ultrafiltration technology at a laboratory scale. Four membrane materials (poly-ether sulfone, polyacrilonytrile, poly vinylidene fluoride and regenerated cellulose) and 5 Molecular Weight Cut-Off (from 3 to 100 kDa) have been studied at bench laboratory scale using the pilot Rayflow (R) 100, commercialised by Rhodia Orelis. The investigation deals with the ability for membranes to offer a high retention of biochemical compounds (proteins and lipids). Results obtained during adsorption tests showed that the regenerated cellulose material seems to be the most appropriate with regards to pore size reduction due to the protein-adsorption. During the ultrafiltration of...
Tipo: Text Palavras-chave: Fish oil; Regenerated cellulose; Surimi wastewater; Enzymatic hydrolysis; Ultrafiltration.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4359.pdf
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Sensory and aromatic characteristics of tongue sole by-products hydrolysates (Cynoglossus senegalensis) ArchiMer
Sylla, K. S. B.; Berge, Jean-pascal; Prost, Carole; Musabyemariya, B.; Seydi, Mg.
Tongue sole by-products coming from fish-filleting plant were hydrolyzed by Protamex® protease. To identify the future application of hydrolysates, a sensory analysis was carried out.The sensory profile was performed with a jury of 14 specialized judges.11 profiles were found by this panel of tasting. In addition, the aromatic characterization revealed that 57 molecules are responsible for these odours described in sensory analysis.The description of these aromatic compounds opens potential way of valorization of these hydrolysates in human and animal consumption.
Tipo: Text Palavras-chave: Hydrolyse enzymatique; Co-produits; Arôme; Enzymatic hydrolysis; By-products; Aroma.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00003/11428/7976.pdf
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Soft liquefaction of the red seaweed Grateloupia turuturu Yamada by ultrasound-assisted enzymatic hydrolysis process ArchiMer
Le Guillard, Cecile; Berge, Jean-pascal; Donnay-moreno, Claire; Bruzac, Sandrine; Ragon, Jean-yves; Baron, Regis; Fleurence, Joel; Dumay, Justine.
Ultrasound-assisted enzymatic hydrolysis is a recent process, increasingly employed for plant biomass liquefaction and the recovery of soluble biomolecules. However, to our knowledge, it has never been used on seaweeds, particularly wet ones. The aim of this study was to compare the efficiency of three processes on the liquefaction of the red seaweed Grateloupia turuturu Yamada: enzyme-assisted extraction (EAE), ultrasound-assisted extraction (UAE), and their combination, ultrasound-assisted enzymatic hydrolysis (UAEH). These comparisons will allow the identification as to which process achieves the highest extraction yield of water-soluble compounds. For this purpose, experiments were conducted at 40 °C for 6 h using an enzymatic cocktail of four...
Tipo: Text Palavras-chave: Ultrasound-assisted enzymatic hydrolysis; Enzymatic hydrolysis; Ultrasound; Liquefaction; Algae; Grateloupia turuturu.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00308/41877/44316.pdf
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Sugarcane bagasse as feedstock for cellulase production by Trichoderma harzianum in optimized culture medium Electron. J. Biotechnol.
Rocha,Vanessa Alves Lima; Maeda,Roberto Nobuyuki; Santa Anna,Lidia Maria Melo; Pereira,Nei.
This work aimed at the production of cellulases from pretreated sugarcane bagasse by the filamentous fungus Trichoderma harzianum IOC 3844 and their application in the hydrolysis of this same substrate, for a future use in second-generation ethanol production. The production of cellulases was optimized, which resulted in high enzymatic activities after 42 hrs of process in an instrumented bioreactor (CMCase 27,017 U x L-1; FPase 1,225 U x L-1; and &#946;-glucosidase 609 U x L-1). The enzymatic extract was concentrated by using a hollow fiber membrane filtration system. The concentrated extract was applied in the hydrolysis of pretreated sugarcane bagasse, after 28 hrs of enzymatic reaction, displaying a similar catalytic performance of that attained...
Tipo: Journal article Palavras-chave: Electrophoresis; Enzymatic hydrolysis; Second generation ethanol; Trichoderma harzianum.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500012
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Valorization of the Macroalgae Sargassum Muticum by Enzymatic Hydrolysis, Interest of Surfactants to Improve the Extraction of Phlorotannins and Polysaccharides ArchiMer
Vandanjon, Laurent; Vallet, Laurent; Le Glatin, Titouan; Deleris, Paul; Baron, Regis; Bourseau, Patrick; Dumay, Justine.
The use of surfactants to improve enzymatic hydrolysis of the macroalgae Sargassum muticum has been investigated. Visible absorption spectroscopy has been used to quantify the solubilization of both polysaccharides and phlorotannins in the hydrolysates.   After total extraction, results showed that Sargassum muticum contained 2.74% (expressed in percent of the dry weight of the algae) of phlorotannins whose 32 % were in the cell wall. This result shows that it is important to access to the parietal phlorotannins. To reach this objective, we chose the enzymatic approach for destructurating the cell wall of the algae. The use of 5% dry weight (DW - 5% by weight of hydrolyzed algae) of an enzymatic mix containing a commercial beta-glucanase, a commercial...
Tipo: Text Palavras-chave: Brown macroalgae; Sargassum muticum; Enzymatic hydrolysis; Surfactants; Phlorotannins.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00350/46105/46073.pdf
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Water ratio effect on the proteins hydrolysis tongue sole by-products (Cynoglossus senegalensis) ArchiMer
Sylla, K. S. B.; Musabyemariya, B.; Berge, Jean-pascal; Seydi, Mg.
In this study the effectiveness of four different water ratio to hydrolyze sole by-products by using Protamex® were compared. Average proteins yields and degree of hydrolysis (DH %) of different hydrolysates were measured, and their peptide profiles were analysed by using gel filtration chromatography. It was found that by changing the hydrolysis conditions it was possible to produce hydrolysates with the same proportion of proteins (60%) and very different in peptide composition (from 294 to 13700 Da).
Tipo: Text Palavras-chave: Coproduits; Eau; Hydrolyse enzymatique; Protéines; By-products; Water; Enzymatic hydrolysis; Proteins.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/00015/12620/9505.pdf
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