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Registros recuperados: 11
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Age-related Changes in DNA Methylation Status of hTERT Gene Promoter of Oral Epithelial Cells 52
Bezerra,Stephane Flaviane de Oliveira; Costa,Ludimila de Araújo; Freitas,Priscylla Alves Nascimento de; Oliveira,Naila Francis Paulo de.
The purpose of this study was to investigate the effect of aging on the DNA methylation status of two genes involved in tumorigenesis (telomerase gene hTERT and DNA repair gene- MLH1) and one in metabolism (methylenetetrahydrofolate reductase gene- MTHFR) in oral epithelial cells. DNA methylation analysis was performed by Methylation Sensitive Restriction Enzymes (MSRE) of healthy oral epithelial cells of child (6-10 years, n=21), young (20-25 years, n=19) and elderly (over 60 years, n=25). The results for the hTERT gene showed significant variation in the methylation frequency at CpG dinucleotides among the groups (p=0.0001), with the methylated condition more frequently in children and young people. In relation to MLH1 and MTHFR, no differences were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epigenetic; DNA methylation; Aging; DNA repair; Telomerase; MTHFR.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100082
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Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil 48
Almeida,Fabiana Greyce Oliveira; Aquino,Priscila Ferreira de; Souza,Afonso Duarte Leäo de; Souza,Antonia Queiroz Lima de; Vinhote,Sonia do Carmo; Mac-Cormick,Thais Messias; Silva,Marcelo Soares da Mota; Chalub,Sidney Raimundo Silva; Fischer,Juliana de Saldanha da Gama; Carvalho,Paulo Costa; Carvalho,Maria da Gloria da Costa.
BACKGROUND: DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylation pattern from five colorectal cancer patients from the Amazon state in Brazil for four tumor suppressor genes, viz.: DAPK, CDH1, CDKN2A, and TIMP2 by employing a polymerase chain reaction (PCR) specific to methylation. Efforts in the study of colorectal cancer are fundamental as it is the third most of highest incidence in the world. RESULTS: Tumor biopsies were methylated in 1/5 (20 %), 2/5 (40 %), 4/5 (80 %), and 4/5 (80 %) for CDH1, CDKN2A, DAPK, and TIMP2...
Tipo: Journal article Palavras-chave: Methylation; Colorectal cancer; Amazon; Epigenetic.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100050
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Constitutional and somatic methylation status of DMRH19 and KvDMR in Wilms tumor patients 74
Cardoso,Leila C.A.; Castaño,Jair A. Tenorio; Pereira,Hanna S.; Lima,Maria Angélica de F.D.; Santos,Anna Cláudia E. dos; Faria,Paulo S. de; Ferman,Sima; Seuánez,Héctor N.; Nevado,Julián B.; Almeida,José Carlos Cabral de; Lapunzina,Pablo; Vargas,Fernando R..
The most frequent epigenetic alterations in Wilms tumor (WT) occur at WT2, assigned to 11p15. WT2 consists of two domains: telomeric domain 1 (DMRH19) that contains the IGF2 gene and an imprinted maternally expressed transcript (H19) and centromeric domain 2 (KvDMR) that contains the genes KCNQ1, KCNQ1OT1 and CDKN1C. In this work, we used pyrosequencing and MS-MLPA to compare the methylation patterns of DMRH19/KvDMR in blood and tumor samples from 40 WT patients. Normal constitutional KvDMR methylation indicated that most of the epigenetic alterations in WT occur at DMRH19. Constitutional DMRH19 hypermethylation (HM DMRH19) was observed in two patients with Beckwith-Wiedemann syndrome. Pyrosequencing and MS-MLPA showed HM DMRH19 in 28/34 tumor samples:...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epigenetic; Histopathology; Methylation; MS-MLPA; Pyrosequencing.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500002
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Effect of Thermal Embryonic Manipulation on the Quality of Male and Female Broiler Meat Submitted to Thermal Stress Pre-Slaughter 89
Fernandes,JIM; Santos,TC; Kaneko,IN; Horn,D; Leyter,JR; Pasa,CLB.
ABSTRACT Adverse environmental conditions during rearing may negatively affect productivity and meat quality of the modern fast-growing broiler strains. Temperature manipulation during sensitive embryonic development periods may affect broilers' physiological responses to environmental conditions during rearing on commercial farms. The objective of this trial was to evaluate the effect of temperature manipulation during incubation and breeder age on the meat quality of male and female broilers submitted to heat stress during the pre-slaughter period. In this experiment, 1280 broiler chicks were distributed according to a completely randomized experimental design in a 2x2x2 factorial arrangement. Treatments consisted of two breeder ages (30 and 60 weeks),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adaptation; Epigenetic; Incubation; Thermoregulation.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200343
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Effects of a parental exposure to diuron on Pacific oyster spat methylome 5
Rondon Sallan, Rodolfo; Grunau, Christoph; Fallet, Manon; Charlemagne, Nicolas; Sussarellu, Rossana; Chaparro, Cristian; Montagnani, Caroline; Mitta, Guillaume; Bachere, Evelyne; Akcha, Farida; Cosseau, Celine.
Environmental epigenetic is an emerging field that studies the cause-effect relationship between environmental factors and heritable trait via an alteration in epigenetic marks. This field has received much attentions since the impact of environmental factors on different epigenetic marks have been shown to be associated with a broad range of phenotypic disorders in natural ecosystems. Chemical pollutants have been shown to affect immediate epigenetic information carriers of several aquatic species but the heritability of the chromatin marks and the consequences for long term adaptation remain open questions. In this work, we investigated the impact of the diuron herbicide on the DNA methylation pattern of spat from exposed Crassotrea gigas genitors. This...
Tipo: Text Palavras-chave: Crassostrea gigas; Mollusk; DNA methylation; Herbicide; Epigenetic.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00602/71413/69895.pdf
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Epigenetic inheritance and intergenerational effects in mollusks 5
Fallet, Manon; Luquet, Emilien; David, Patrice; Cosseau, Céline.
Recent insights in evolutionary biology have shed light on epigenetic variation that interacts with genetic variation to convey heritable information. An important characteristic of epigenetic changes is that they can be produced in response to environmental cues and passed on to later generations, potentially facilitating later genetic adaptation. While our understanding of epigenetic mechanisms in vertebrates is rapidly growing, our knowledge about invertebrates remains lower, or is restricted to model organisms. Mollusks in particular, are a large group of invertebrates, with several species important for ecosystem function, human economy and health. In this review, we attempt to summarize the literature on epigenetic and intergenerational studies in...
Tipo: Text Palavras-chave: Mollusk; Epigenetic; Intergenerational effect; Phenotypic plasticity; Adaptation.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00590/70163/68170.pdf
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Evaluation of Placental Epigenetic Changes due to Morphine Consumption 77
Kazemi,Masoomeh; Tekieh,Elaheh; Golabi,Sahar; Sahraei,Hedayat.
Based on previous studies, a variety of bioenvironmental elements including inappropriate nutrition, diseases, infections, stressors, and medications are involved in epigenetic changes. Drug abuse is one of the most important causes of epigenetic changes and a concern in today's world. Studies have shown that morphine use by pregnant mothers causes several disorders in mothers in addition to transferring abnormalities to the next generation (placenta and embryo). Epigenetic factors such as morphine cause changes in gene expression in placenta as the first embryonic defense barrier. Because placenta does all the nutritional exchanges between mother's and embryo's blood, placental health guarantees normal embryonic development. Many studies have been...
Tipo: Journal article Palavras-chave: Development; Morphine; Embryo; Epigenetic; Pregnancy; Placenta.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100036
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Evolution of DNMT2 in drosophilids: Evidence for positive and purifying selection and insights into new protein (pathways) interactions 74
Vieira,Gilberto Cavalheiro; D’Ávila,Marícia Fantinel; Zanini,Rebeca; Deprá,Maríndia; da Silva Valente,Vera Lúcia.
Abstract The DNA methyltransferase 2 (DNMT2) protein is the most conserved member of the DNA methyltransferase family. Nevertheless, its substrate specificity is still controversial and elusive. The genomic role and determinants of DNA methylation are poorly understood in invertebrates, and several mechanisms and associations are suggested. In Drosophila, the only known DNMT gene is Dnmt2. Here we present our findings from a wide search for Dnmt2 homologs in 68 species of Drosophilidae. We investigated its molecular evolution, and in our phylogenetic analyses the main clades of Drosophilidae species were recovered. We tested whether the Dnmt2 has evolved neutrally or under positive selection along the subgenera Drosophila and Sophophora and investigated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila; Dnmt2; Positive selection; Epigenetic; Positive-destabilizing selection.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200215
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Insights on the epigenetic mechanisms underlying pulmonary arterial hypertension 56
Luna,R.C.P.; de Oliveira,Y.; Lisboa,J.V.C.; Chaves,T.R.; de Araújo,T.A.M.; de Sousa,E.E.; Miranda Neto,M.; Pirola,L.; Braga,V.A.; de Brito Alves,J.L..
Pulmonary arterial hypertension (PAH), characterized by localized increased arterial blood pressure in the lungs, is a slow developing long-term disease that can be fatal. PAH is characterized by inflammation, vascular tone imbalance, pathological pulmonary vascular remodeling, and right-sided heart failure. Current treatments for PAH are palliative and development of new therapies is necessary. Recent and relevant studies have demonstrated that epigenetic processes may exert key influences on the pathogenesis of PAH and may be promising therapeutic targets in the prevention and/or cure of this condition. The aim of the present mini-review is to summarize the occurrence of epigenetic-based mechanisms in the context of PAH physiopathology, focusing on the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epigenetic; Pulmonary arterial hypertension; DNA Methylation; Histone acetylation; MiRNAs.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200301
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Multi- and transgenerational effects following early-life exposure of zebrafish to permethrin and coumarin 47: Impact on growth, fertility, behavior and lipid metabolism 5
Blanc, Mélanie; Cormier, Bettie; Hyötyläinen, Tuulia; Krauss, Martin; Scherbak, Nikolai; Cousin, Xavier; Keiter, Steffen H..
Transgenerational effects induced by environmental stressors are a threat to ecosystems and human health. However, there is still limited observation and understanding of the potential of chemicals to influence life outcomes over several generations. In the present study, we investigated the effects of two environmental contaminants, coumarin 47 and permethrin, on exposed zebrafish (F0) and their progeny (F1–F3). Coumarin 47 is commonly found in personal care products and dyes, whereas permethrin is used as a domestic and agricultural pyrethroid insecticide/insect repellent. Zebrafish (F0) were exposed during early development until 28 days post-fertilization and their progeny (F1–F3) were bred unexposed. On one hand, the effects induced by coumarin 47...
Tipo: Text Palavras-chave: Pyrethroid; Endocrine disrupter; Epigenetic; Transgenerational; Neurotoxic.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00652/76393/77411.pdf
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The MTHFR C677T polymorphism and global DNA methylation in oral epithelial cells 74
Arruda,Isabela Tatiana Sales de; Persuhn,Darlene Camati; Oliveira,Naila Francis Paulo de.
DNA methylation is mediated by DNA methyltransferases (DNMTs) that add a methyl group to the 5'-carbon of cytosine. The enzyme methylenetetrahydrofolate reductase (MTHFR) catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the rate-limiting step of the cycle involving the methyl donor S-adenosyl-L-methionine (SAM). The MTHFR C677T polymorphism results in a thermolabile enzyme with reduced activity that is predicted to influence the DNA methylation status. In this study, we investigated the impact of the MTHFR C677T polymorphism on the global DNA methylation of oral epithelial cells obtained from 54 healthy subjects. There were no significant differences in global DNA methylation among the MTHFR CC, CT and TT genotypes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA methylation; Epigenetic; MTHFR C677T; Oral epithelial cells; Polymorphism.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400004
Registros recuperados: 11
Primeira ... 1 ... Última
 

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