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Registros recuperados: 41
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Absence of CpG observed/expected ratio depletion in Haemonchus contortus evidences a non-methylated genome. Repositório Alice
NICIURA, S. C. M.; THOLON, P..
Editores: Luiz Lehmann Coutinho, ESALQ/USP; Luciana Correia de Almeida Regitano, Embrapa Pecuária Sudeste; Gerson Barreto Mourão, ESALQ/USP; Aline Silva Mello Cesar, ESALQ/USP; Bárbara Silva Vignato, FZEA/USP; Mirele Daiana Poleti, ESALQ/USP; Wellison Jarles da Silva Diniz, UFSCar.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Epigenetics; DNA methylation; Gastrointestinal nematode; Barber?s pole worm.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1075655
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Age- and quality-dependent DNA methylation correlate with melanin-based coloration in a wild bird ArchiMer
Soulsbury, Carl D.; Lipponen, Anssi; Wood, Kristie; Mein, Charles A.; Hoffman, Joseph I.; Lebigre, Christophe.
Secondary sexual trait expression can be influenced by fixed individual factors (such as genetic quality) as well as by dynamic factors (such as age and environmentally induced gene expression) that may be associated with variation in condition or quality. In particular, melanin-based traits are known to relate to condition and there is a well-characterized genetic pathway underpinning their expression. However, the mechanisms linking variable trait expression to genetic quality remain unclear. One plausible mechanism is that genetic quality could influence trait expression via differential methylation and differential gene expression. We therefore conducted a pilot study examining DNA methylation at a candidate gene (agouti-related neuropeptide: AgRP) in...
Tipo: Text Palavras-chave: AgRP; Coloration; Epigenetics; Heterozygosity; Melanin; Sexual ornament.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00452/56391/58029.pdf
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Artificially induced autopolyploidy increases genome-wide methylation in grapevine. Repositório Alice
BOSCO, D. D.; SINSKI, I.; MAIA, J. D. G.; RITSCHEL, P. S.; QUECINI, V..
Epigenetic processes are reversible chemical modifications acting on two genetic information-containing targets: DNA, and histones.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Antimitotic agent; Epigenetics; Oryzalin; Tetraploids; Vitis.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1083008
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Assessment of changes in the brca2 and p53 genes in breast invasive ductal carcinoma in northeast Brazil Biol. Res.
Ramalho,Eduardo AVF; Silva-Filho,João LQ; Cartaxo,Marina FS; Cavalcanti,Carmelita BL; Rêgo,Moacyr JBM; Oliveira,Maria BM; Beltrão,Eduardo IC.
BACKGROUND: BRCA protein interacts with at least 13 different proteins that have been implicated with cancer susceptibility and loss of BRCA function is correlated to sensitivity to DNA crosslinking agents in preclinical models. RESULTS: BRCA2 methylation frequency was 44%, p53 Pro22 allele frequency was 32% and heterozygous frequency of Arg/Pro72 genotype was 60% which could be associated as risk factor for metastasis (p = 0.046 OR = 4.190). Regarding to polymorphism of codon 249 the frequency of Arg249 allele presented 82% which was considered not statistically significant. CONCLUSIONS: There was not statistical significance to BRCA2 promoter methylation with any parameters chosen. However, our findings suggest that patients who present heterozygous...
Tipo: Journal article Palavras-chave: Breast cancer; Epigenetics; Metastasis; Methylation; Polymorphism.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100003
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BASiNET - Biological Sequences NETwork: a case study on coding and non-coding RNAs identification. Repositório Alice
ITO, E. A.; KATAHIRA, I.; VICENTE, F. F. da R.; PEREIRA, L. F. P.; LOPES, F. M..
With the emergence of Next Generation Sequencing (NGS) technologies, a large volume of sequence data in particular de novo sequencing was rapidly produced at relatively low costs. In this context, computational tools are increasingly important to assist in the identification of relevant information to understand the functioning of organisms. This work introduces BASiNET, an alignment-free tool for classifying biological sequences based on the feature extraction from complex network measurements. The method initially transform the sequences and represents them as complex networks. Then it extracts topological measures and constructs a feature vector that is used to classify the sequences. The method was evaluated in the classification of coding and...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: RNA-seq; Neurodegenerative diseases; Cardiovascular diseases; Epigenetics; Nucleotides.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108754
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BjussuLAAO-II induces cytotoxicity and alters DNA methylation of cell-cycle genes in monocultured/co-cultured HepG2 cells J. Venom. Anim. Toxins incl. Trop. Dis.
Machado,Ana Rita Thomazela; Aissa,Alexandre Ferro; Ribeiro,Diego Luis; Ferreira Jr.,Rui Seabra; Sampaio,Suely Vilela; Antunes,Lusânia Maria Greggi.
Abstract Background: The use of animal venoms and their toxins as material sources for biotechnological applications has received much attention from the pharmaceutical industry. L-amino acid oxidases from snake venoms (SV-LAAOs) have demonstrated innumerous biological effects and pharmacological potential against different cancer types. Hepatocellular carcinoma has increased worldwide, and the aberrant DNA methylation of liver cells is a common mechanism to promote hepatic tumorigenesis. Moreover, tumor microenvironment plays a major role in neoplastic transformation. To elucidate the molecular mechanisms responsible for the cytotoxic effects of SV-LAAO in human cancer cells, this study aimed to evaluate the cytotoxicity and the alterations in DNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Snake venom; Epigenetics; GADD45A; CCND1; CDKN1A.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100305
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Cardio-respiratory development in bird embryos: new insights from a venerable animal model R. Bras. Zootec.
Burggren,Warren W.; Santin,Josele Flores; Antich,Maria Rojas.
ABSTRACT The avian embryo is a time-honored animal model for understanding vertebrate development. A key area of extensive study using bird embryos centers on developmental phenotypic plasticity of the cardio-respiratory system and how its normal development can be affected by abiotic factors such as temperature and oxygen availability. Through the investigation of the plasticity of development, we gain a better understanding of both the regulation of the developmental process and the embryo's capacity for self-repair. Additionally, experiments with abiotic and biotic stressors during development have helped delineate not just critical windows for avian cardio-respiratory development, but the general characteristics (e.g., timing and dose-dependence) of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bird embryos; Cardiovascular; Epigenetics; Fetal programing; Respiratory plasticity; Toxicants.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016001100709
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Challenges and perspectives to enhance cattle production via in vitro techniques: focus on epigenetics and cell-secreted vesicles Ciência Rural
Bressan,Fabiana Fernandes; Fantinato-Neto,Paulo; Andrade,Gabriella Mamede; Sangalli,Juliano Rodrigues; Sampaio,Rafael Vilar; Silveira,Juliano Coelho da; Perecin,Felipe; Meirelles,Flávio Vieira.
This review aim to present some clinical problems found in IVP-derived animals focusing on NT procedures and to discuss the possible role of epigenetics in such process. Also, as cell-secreted vesicles have been reported as possible regulators of important physiological reproductive processes such as folliculogenesis and fertilization, it is also presented herein a new perspective of manipulating the pre-implantation period trough effector molecules contained in such vesicles.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryo development; Nuclear transfer; Epigenetics; Extra-cellular vesicles..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001001879
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Characterisation of the methylation pattern in the intragenic CpG island of the IGF2 gene in Bos taurus indicus cumulus cells during in vitro maturation. Repositório Alice
FRANCO, M. M.; FAGUNDES, N. S.; MICHALCZECHEN-LACERDA, V. A.; CAIXETA, E. S.; RODRIGUES, F. de C.; MACHADO, G. M.; FERREIRA, A. R.; DODE, M. A. N..
bitstream/item/180017/1/Franco2014-Article-CharacterisationOfTheMethylati.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cumulus cells; IGF2; In vitromaturation; Bovine; DNA methylation; Epigenetics.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/995151
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ChIP-seq analysis of histone H3K9 trimethylation in peripheral blood mononuclear cells of membranous nephropathy patients BJMBR
Sui,W.G.; He,H.Y.; Yan,Q.; Chen,J.J.; Zhang,R.H.; Dai,Y..
Membranous nephropathy (MN), characterized by the presence of diffuse thickening of the glomerular basement membrane and subepithelial in situimmune complex disposition, is the most common cause of idiopathic nephrotic syndrome in adults, with an incidence of 5-10 per million per year. A number of studies have confirmed the relevance of several experimental insights to the pathogenesis of human MN, but the specific biomarkers of MN have not been fully elucidated. As a result, our knowledge of the alterations in histone methylation in MN is unclear. We used chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) to analyze the variations in a methylated histone (H3K9me3) in peripheral blood mononuclear cells from 10 MN patients and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ChIP-seq; Epigenetics; H3K9me3; Membranous nephropathy.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100042
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Cytosine methylation of mature microRNAs inhibits their functions and is associated with poor prognosis in glioblastoma multiforme ArchiMer
Cheray, Mathilde; Etcheverry, Amandine; Jacques, Camille; Pacaud, Romain; Bougras-cartron, Gwenola; Aubry, Marc; Denoual, Florent; Peterlongo, Pierre; Nadaradjane, Arulraj; Briand, Josephine; Akcha, Farida; Heymann, Dominique; Vallette, François M.; Mosser, Jean; Ory, Benjamin; Cartron, Pierre-françois.
Background Literature reports that mature microRNA (miRNA) can be methylated at adenosine, guanosine and cytosine. However, the molecular mechanisms involved in cytosine methylation of miRNAs have not yet been fully elucidated. Here we investigated the biological role and underlying mechanism of cytosine methylation in miRNAs in glioblastoma multiforme (GBM). Methods RNA immunoprecipitation with the anti-5methylcytosine (5mC) antibody followed by Array, ELISA, dot blot, incorporation of a radio-labelled methyl group in miRNA, and miRNA bisulfite sequencing were perfomred to detect the cytosine methylation in mature miRNA. Cross-Linking immunoprecipiation qPCR, transfection with methylation/unmethylated mimic miRNA, luciferase promoter reporter plasmid,...
Tipo: Text Palavras-chave: MiRNA; Cytosine-methylation; Epigenetics; DNMT3A; AGO4; Glioblastoma; Epitranscriptomics.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00610/72196/70980.pdf
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Detection of differentially methylated regions of irradiated fig tree selections Scientia Agricola
Rodrigues,Maria Gabriela Fontanetti; Soares,Murilo Racy; Gimenez,Daniele Fernanda Jovino; Fonseca,Larissa Fernanda Simielli; Torrieri,Erico; Ramos,Ester Silveira; Giuliatti,Silvana.
ABSTRACT Fig tree (Ficus carica L.) breeding programs using conventional methods, such as directed crosses, to obtain new cultivars, are unworkable in many countries, including Brazil. Consequently, genetic breeding through mutagenesis has emerged as an important line of research that can improve this crop, and be a significant source of information about this species and assist in the implementation of propagation projects and appropriate management. The aim of this study was to verify the existence of epigenetic variability attributable to DNA methylation in irradiated fig selections when compared both to each other and to the main commercial cultivar, “Roxo-de-Valinhos”, which had previously used methylation-sensitive amplified polymorphism (MSAP) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ficus carica; DMRs; Methylation; Plant breeding; Epigenetics.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000400285
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Differential expression of the MHM region and of sex-determining-related genes during gonadal development in chicken embryos. Repositório Alice
CAETANO, L. C.; GENNARO, F. G. O.; COELHO, K.; ARAÚJO, F. M.; VILA, R. A.; ARAÚJO, A.; BERNARDO, A. de M.; MARCONDES, C. R.; LOPES, S. M. C. de S.; RAMOS, E. S..
The chicken (Gallus gallus) embryo has been used as a classic model system for developmental studies because of its easy accessibility for surgical manipulation during embryonic development. Sex determination in birds is chromosomally based (ZZ for males and ZW for females); however, the basic mechanism of sex determination is still unknown. Here, the dynamics of expression of candidate genes implicated in vertebrate sex determination and differentiation were studied during embryonic chicken gonadal development. Gene expression profiles were obtained before, during, and after gonadal sex differentiation in females and males for DMRT1, SOX3, SOX9, DAX MHM and sex-determining gene expression in chicken embryos SCII, HINTZ, HINTW, and the male hypermethylated...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Gonad; Development; Chicken; Epigenetics; Dosage compensation.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/985036
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DNA methylation of the endogenous retrovirus Fematrin-1 in fetal placenta is associated with survival rate of cloned calves. Repositório Alice
SILVEIRA, M. M.; VARGAS, L. N.; BAYÃO, H. X. S.; SCHUMANN, N. A. B.; CAETANO, A. R.; RUMPF, R.; FRANCO, M. M..
bitstream/item/203972/1/1-s2.0-S0143400419306708-main.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Transposable elements; Retrotransposon; Envelope protein; Placental morphogenesis; Cloning; Epigenetics.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113684
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Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of Cattleya tigrina A. Richard Biological Sciences
Almeida, Vivian; Fraga, Hugo Pacheco de Freitas; Navarro, Bruno Bachiega; Guerra, Miguel Pedro; Pescador, Rosete.
Cattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA methylation levels and free polyamines during protocorm-like bodies (PLBs) induction of C. tigrina. Along with that, an efficient protocol for in vitro propagation of this species is proposed. The first evidences of PLBs induction in C. tigrina occurred at seven days in culture, starting from the basal portion of the leaf abaxial surface. A hypomethylation marked the beginning of cell differentiation, followed by an increased global DNA methylation at 35 days in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.03.00.00-0; 2.03.03.00-9; 2.03.06.00-8 orchids; Micropropagation; In vitro culture; Epigenetics; PLBs. Botânica.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36656
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Effects of trichostatina, a chromatin-modifying agent, on meiotic progression of bovine oocytes and embryonic development. Repositório Alice
SARAIVA, N. Z.; OLIVEIRA, C. L.; DEL COLLADO, C. L.; LIMA, M. R. de; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Epigenetics; Trichostatin A; Oocyte competence.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/975148
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Efficacy of the dietary histone deacetylase inhibitor butyrate alone or in combination with vitamin A against proliferation of MCF-7 human breast cancer cells BJMBR
Andrade,F.O.; Nagamine,M.K.; Conti,A. De; Chaible,L.M.; Fontelles,C.C.; Jordão Junior,A.A.; Vannucchi,H.; Dagli,M.L.Z.; Bassoli,B.K.; Moreno,F.S.; Ong,T.P..
The combined treatment with histone deacetylase inhibitors (HDACi) and retinoids has been suggested as a potential epigenetic strategy for the control of cancer. In the present study, we investigated the effects of treatment with butyrate, a dietary HDACi, combined with vitamin A on MCF-7 human breast cancer cells. Cell proliferation was evaluated by the crystal violet staining method. MCF-7 cells were plated at 5 x 10(4) cells/mL and treated with butyrate (1 mM) alone or combined with vitamin A (10 µM) for 24 to 120 h. Cell proliferation inhibition was 34, 10 and 46% following treatment with butyrate, vitamin A and their combination, respectively, suggesting that vitamin A potentiated the inhibitory activities of butyrate. Furthermore, exposure to this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Breast cancer; MCF-7 cells; Butyric acid; Vitamin A; Epigenetics.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900008
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Epigenetic alterations in human brain tumors in a Brazilian population Genet. Mol. Biol.
Anselmo,Nilson Praia; Bello,Maria Josefa; Gonzalez-Gomez,Pilar; Dias,Luis Antonio Araújo; Almeida,José Reinaldo Walter de; Santos,Marcelo José dos; Rey,Juan A.; Casartelli,Cacilda.
Aberrant methylation of CpG islands located in promoter regions represents one of the major mechanisms for silencing cancer-related genes in tumor cells. We determined the frequency of aberrant CpG island methylation for several tumor-associated genes: DAPK, MGMT, p14ARF, p16INK4a, TP73, RB1 and TIMP-3 in 55 brain tumors, consisting of 26 neuroepithelial tumors, 6 peripheral nerve tumors, 13 meningeal tumors and 10 metastatic brain tumors. Aberrant methylation of at least one of the seven genes studied was detected in 83.6% of the cases. The frequencies of aberrant methylation were: 40% for p14ARF, 38.2% for MGMT, 30.9% for, p16INK4a, 14.6% for TP73 and for TIMP-3, 12.7% for DAPK and 1.8% for RB1. These data suggest that the hypermethylation observed in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brain tumors; Epigenetics; Methylation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300001
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Epigenetic aspects of sexual and asexual seed development. Repositório Alice
RODRIGUES, J. C. M.; KOLTUNOW, A. M. G..
bitstream/item/178121/1/ID-27889-1.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Sementes; Desenvolvimento; Reprodução Assexuada; Apomixis; Epigenetics; Seed development.
Ano: 2005 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/188345
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Epigenetic origin of adaptive phenotypic variants in the human blood fluke Schistosoma mansoni ArchiMer
Fneich, Sara; Theron, Andre; Cosseau, Celine; Rognon, Anne; Aliaga, Benoit; Buard, Jerome; Duval, David; Arancibia, Nathalie; Boissier, Jerome; Roquis, David; Mitta, Guillaume; Grunau, Christoph.
Background: Adaptive evolution is not possible without the generation of phenotypic variants. The origin of these variations has been a central topic in evolutionary biology. Up to now, it was commonly accepted that standing genetic variation is the only cause of phenotypic variants. However, epigenetic information is emerging as a complementary source of heritable phenotypic variation that contributes to evolution. The relative importance of genetics and epigenetics in generating heritable phenotypic variation is nevertheless a matter of debate. Results: We used a host-parasite system to address this question. The human blood fluke Schistosoma mansoni can adapt rapidly to new intermediate snail hosts. The interaction between parasite and mollusk is...
Tipo: Text Palavras-chave: Epigenetics; Adaptive evolution; Compatibility polymorphism; Schistosoma mansoni.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00615/72708/71730.pdf
Registros recuperados: 41
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