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	Provedor de dados Ciênc. Tecnol. Aliment. (1) BJMBR (1) Autor Borojevic,R. (1) El-Cheikh,M.C. (1) FIGUEIREDO,Vitória Ribeiro Garcia de (1) GEORGETTI,Sandra Regina (1) Gomes,L. (1) KUROZAWA,Louise Emy (1) MONTILHA,Marina Silveira (1) Pereira,D.A. (1) SBROGGIO,Melissa Ferreira (1) Mais... Palavra-chave Exopeptidase (2) Antioxidant capacity (1) Bioactive peptides (1) Ectoenzyme (1) Endopeptidase (1) Hematopoiesis (1) Membrane rafts (1) Soy pulp (1) Stroma (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2016 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Dipeptidyl peptidase IV (CD26) activity in the hematopoietic system: differences between the membrane-anchored and the released enzyme activity BJMBR Pereira,D.A.; Gomes,L.; El-Cheikh,M.C.; Borojevic,R.. Dipeptidyl peptidase IV (DPP-IV; CD26) (EC 3.4.14.5) is a membrane-anchored ectoenzyme with N-terminal exopeptidase activity that preferentially cleaves X-Pro-dipeptides. It can also be spontaneously released to act in the extracellular environment or associated with the extracellular matrix. Many hematopoietic cytokines and chemokines contain DPP-IV-susceptible N-terminal sequences. We monitored DPP-IV expression and activity in murine bone marrow and liver stroma cells which sustain hematopoiesis, myeloid precursors, skin fibroblasts, and myoblasts. RT-PCR analysis showed that all these cells produced mRNA for DPP-IV. Partially purified protein reacted with a commercial antibody to CD26. The K M values for Gly-Pro-p-nitroanilide ranged from 0.43 to 0.98... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hematopoiesis; Stroma; Exopeptidase; Ectoenzyme; Membrane rafts. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500003 Influence of the degree of hydrolysis and type of enzyme on antioxidant activity of okara protein hydrolysates Ciênc. Tecnol. Aliment. SBROGGIO,Melissa Ferreira; MONTILHA,Marina Silveira; FIGUEIREDO,Vitória Ribeiro Garcia de; GEORGETTI,Sandra Regina; KUROZAWA,Louise Emy. Abstract The objective of this work was to evaluate the antioxidant activity of protein hydrolysates obtained by the enzymatic hydrolysis of okara using an endopeptidase (Alcalase) and exopeptidase (Flavourzyme). The reaction was monitored by the pH-stat procedure in which five aliquots were collected during the hydrolysis by each enzyme, corresponding to different degrees of hydrolysis (DH). The antioxidant activities of the aliquots were evaluated by the ABTS, DPPH and FRAP methods. For the hydrolysates obtained using Alcalase, the antioxidant activities increased from: 68.6 to 99.5% (ABTS), 14.5 to 17.7% (DPPH) and 222.6 to 684.9 µM Trolox (FRAP), when the DH varied from 0 to 33.6%. With respect to Flavourzyme, the results were: 67.2 to 88.2% (ABTS),... Tipo: Info:eu-repo/semantics/article Palavras-chave: Soy pulp; Endopeptidase; Exopeptidase; Antioxidant capacity; Bioactive peptides. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000200375 Registros recuperados: 2 Primeira ... 1 ... Última

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