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Registros recuperados: 40
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Activation of MMPs during experimental infection of goats by CAEV. Repositório Alice
ELOY, A. M. X.; GALIZA, I. S. de; ANDRIOLI, A.; PINHEIRO, R. R..
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: CAEV; Infecção experimental; Brasil; Metaloproteinase; Matrix metalloproteínases; MMPs; Artrite Encefalite Caprina a Virus; Caprino; Doença animal; Caprine arthritis encephalit virus; Goats; Animal diseases; Experimental infection; Brazil.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1073180
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Antifungal activity of the naphthoquinone beta-lapachone against disseminated infection with Cryptococcus neoformans var. neoformans in dexamethasone-immunosuppressed Swiss mice BJMBR
Medeiros,C.S.; Pontes-Filho,N.T.; Camara,C.A.; Lima-Filho,J.V.; Oliveira,P.C.; Lemos,S.A.; Leal,A.F.G.; Brandão,J.O.C.; Neves,R.P..
The in vivo antifungal activity of the naphthoquinone beta-lapachone against disseminated infection by Cryptococcus neoformans was investigated. Swiss mice were immunosuppressed daily with dexamethasone (0.5 mg per mouse) intraperitoneally for 3 days, the procedure was repeated 4 days later, and the animals were then challenged intravenously with C. neoformans (10(6) CFU/mL) 1 week later. Seven days after infection, the mice were divided into groups and treated daily with beta-lapachone (10 mg/kg, iv) for 7 (N = 6) and 14 days (N = 10). Amphotericin B (0.5 mg/kg) was used as comparator drug and an additional group received PBS. Treatment with beta-lapachone cleared the yeast from the spleen and liver, and the fungal burden decreased approximately 10(4)...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Beta-lapachone; Cryptococcus neoformans; Cryptococcosis; Immunesuppression; Experimental infection; Amphotericin B.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400004
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Association between polyclonal B cell activation and the presence of autoantibodies in mice infected with Yersinia enterocolitica O:3 BJMBR
Eight-week old conventional female Swiss mice were inoculated intravenously with Yersinia enterocolitica O:3. A second group of normal mice was used as control. Five mice from each group were bled by heart puncture and their spleens were removed for spleen cell collection on the 3rd, 5th, 7th, 10th, 14th and 21st day after infection. Immunoglobulin-secreting spleen cells were detected by the isotype-specific protein A plaque assay. Total immunoglobulin levels were determined in mouse serum by single radial immunodiffusion and the presence of autoantibodies was determined by ELISA. We observed a marked increase in the total number of cells secreting immunoglobulins of all isotypes as early as on the 3rd day post-infection and the peak of secretion occurred...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Yersinia enterocolitica; Polyclonal activation; Experimental infection; Autoantibodies.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300015
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Bovine respiratory syncytial virus: immunohistochemichal detection in mouse and bovine tissues using a Mab against human respiratory syncytial virus Arq. Bras. Med. Vet. Zootec.
Almeida,R.S.; Spilki,F.R.; Roehe,P.M.; Verinaud,L.M.C.; Arns,C.W..
An immunoistochemical (IHC) test was developed to detect bovine respiratory syncytial virus (BRSV) in cell cultures and tissues of experimentally infected mice and calves, using a commercial monoclonal antibody (Mab) against human respiratory syncytial virus (HRSV), as a less expensive alternative, instead of producing specific monoclonal antibodies to BRSV. Clinical samples from calves suffering respiratory disease were also submitted to this test. IHC detected BRSV antigens in mouse tracheas (3, 5 and 7 days post-infection) and lungs (5 and 7 days post-infection), and in one of three lungs from experimentally infected calves. Lungs samples from two naturally infected calves were tested and resulted positive for BRSV by the IHC test. These results suggest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine respiratory syncytial virus; Immunohistochemistry; Calf; Mice; Experimental infection; Monoclonal antibody.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600001
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Clinical and virological characteristics of calves experimentally infected with a Brazilian isolate of bovine viral diarrhea virus type 1a Ciência Rural
Quadros,Luana Marchi; Jardim,José Conrado dos Santos; Merchioratto,Ingryd; Brum,Mário Celso Sperotto.
ABSTRACT: To study the pathogenicity of the Brazilian bovine viral diarrhea virus (BVDV) type 1a 241.10 isolate, four calves were intranasally inoculated with a viral suspension containing 107.2 TCID50 mL-1. One calf was left uninoculated and kept in contact with the other calves to investigate viral transmissibility. After inoculation, the animals were monitored daily for clinical signs of infection. The presence of the virus in the blood and nasal secretions was confirmed by virus isolation in cell culture. White blood cells were quantified prior to and every 3 days after infection, and the presence of antibodies was checked every 7 days, starting at day 0 until day 42 post-inoculation (pi). After infection, nasal and ocular serous secretions were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calf; Experimental infection; Pestivirus; Bovine; BVDV.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001101986
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Coccidiose em cabritos associada a infecção experimental com Eimeria ninakohlyakimovae. Repositório Alice
VIEIRA, L. da S.; LIMA, J. D.; SILVA, M. B. O..
Este trabalho descreve a sintomatologia clinica e as alteracoes causadas por E. ninakohlyakimovae em caprinos jovens experimentalmente infectados.
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Infecção experimental; Eimeriosis; Caprino; Cabrito; Eimeriose; Doença animal; Goats; Eimeria ninakohlyakimovae; Artificial infection; Experimental infection.
Ano: 1996 URL: http://www.alice.cnptia.embrapa.br/handle/doc/514755
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Coccidiosis in goats experimentally infected with Eimeria ninakohlyakimovae Yakimoff and Rastegaieff, 1930 emend. Levine, 1961. Repositório Alice
VIEIRA, L. da S.; LIMA, J. D.; SILVA, M. B. O.; TOLENTINO, A. C. V.; BOTELHO, A. C. C..
Forty-three goats aged one to three weeks experimentally infected with Eimeria ninakohlyakimovae were observed from the first to the 24th day after infection (DAI) Animals inoculated with 2.0 x 1e5 oocysts/kg body weight presented diarrheal feces with a fetid odor, dark brown coloration and presence of blood and pieces of intestinal mucosa, loss of appetite, dehydration, mussed fur and general organic weakness. The macroscopic lesions observed were thickening of the cecum and colon mucosa, edema, hemorrhage and hyperemia. Histological examination showed desquamation of the crypt epithelium, with severe hemorrhagic enteritis, edema and necrosis.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Infecção experimental; Experimental infection; Caprino; Coccidiose; Goats; Eimeria ninakohlyakimovae; Coccidia; Experimental infection.
Ano: 1996 URL: http://www.alice.cnptia.embrapa.br/handle/doc/514871
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Contribution of in Vivo Experimental Challenges to Understanding Flat Oyster Ostrea edulis Resistance to Bonamia ostreae ArchiMer
Morga, Benjamin; Renault, Tristan; Faury, Nicole; Lerond, Sophie; Garcia, Celine; Chollet, Bruno; Joly, Jean-pierre; Lapegue, Sylvie; Harrang, Estelle; Arzul, Isabelle.
Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and...
Tipo: Text Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Resistance; Experimental infection; Haemocytes; Gene expression; Apoptosis.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00405/51665/52213.pdf
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Crassostrea gigas mortality in France: the usual suspect, a herpes virus, may not be the killer in this polymicrobial opportunistic disease ArchiMer
Petton, Bruno; Bruto, Maxime; James, Adele; Labreuche, Yannick; Alunno-bruscia, Marianne; Le Roux, Frederique.
Successive disease outbreaks in oyster (Crassostrea gigas) beds in France have resulted in dramatic losses in production, and subsequent decline in the oyster-farming industry. Deaths of juvenile oysters have been associated with the presence of a herpes virus (OsHV-1 µvar) and bacterial populations of the genus Vibrio. Although the pathogenicity of OsHV-1 µvar, as well as several strains of Vibrio has been demonstrated by experimental infections, our understanding of the complexity of infections occurring in the natural environment remains limited. In the present study, we use specific-pathogen-free (SPF) oysters infected in an estuarine environment to study the diversity and dynamics of cultured microbial populations during disease expression. We observe...
Tipo: Text Palavras-chave: Pacific oysters; Summer mortality; Herpes virus; Vibrio pathogenicity; Experimental infection.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00271/38220/36377.pdf
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Cytokine gene expression and molecular detection of Mycobacterium avium subspecies paratuberculosis in organs of experimentally infected mice. Repositório Alice
SCHWARZ, D. G. G.; PIETRALONGA, P. A. G.; SOUZA, M. C. C.; CARVALHO, I. A.; CRUZEIRO, R. S.; MALAQUIAS, J. V.; BENJAMIM, L. A.; SILVA JÚNIOR, A.; MOREIRA, M. A. S..
Resumo - Mycobacterium avium subespécie paratuberculosis (MAP) pode infectar ruminantes e permanecer subclínica por longos períodos nos rebanhos. A identificação de órgãos mais susceptíveis à infecção e a avaliação da expressão das citocinas no local da infecção são importantes para compreender a patogênese de MAP. Neste estudo foi avaliada a probabilidade de detecção de DNA de MAP e a expressão de citocinas em órgãos de camundongos C57BL/6 infectados por via intraperitoneal durante 120 dias. Dentre os órgãos avaliados, o baço (85%), cólon (75%) e fígado (60%) tiveram as maiores frequências de positividade. Quando comparadas essas frequências entre os órgãos, verificou-se que o baço teve 1,54 vezes mais probabilidade de ser positivo em relação ao íleo, e...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Mycobacterium avium; Mice; Johne?s disease; Experimental infection; Doença de Johne; Murinos; Infecção experimental.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1036032
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Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected J. Venom. Anim. Toxins incl. Trop. Dis.
Langoni,H.; Das Dores,C. B.; Silva,R. C.; Pezerico,S. B.; Castro,A. P. B.; Da Silva,A. V..
Detection of Toxoplasma gondii (T. gondii) DNA in blood can help to diagnose the disease in its acute phase; however, it must be considered that hemoglobin, present in blood, can inhibit polymerase activity, making impracticable the detection of DNA in samples. Mice were experimentally infected via oral route with ME49 and BTU2 strains cysts and RH strain tachyzoites; polymerase chain reaction was used to detect T. gondii DNA in mice sera 18, 24, 48, 96, and 192 hours post infection (PI). Toxoplama gondii DNA was detected in only one animal infected with BTU2 strain, genotype III (isolated from a dog with neurological signs) 18 hours PI. The agent's DNA was not detected in any sample of the other experimental groups. New studies must be carried out to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxoplasmosis; PCR; Serum; Experimental infection; Mice.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200004
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Development of Eimeria ninakohlyakimovae Yakimoff & Rastegaieff, 1930 emend. Levine, 1961 in experimentally infected goats. Repositório Alice
VIEIRA, L. da S.; LIMA, J. D.; SANTA ROSA, J..
1996
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Infecção experimental; Eimeria spp; Crescimento; Mucosa intestinal; Caprino; Coccidiose; Goats; Experimental infection; Artificial infection; Eimeria ninakohlyakimovae; Parasitology; Growth; Intestinal mucosa; Gametes.
Ano: 1996 URL: http://www.alice.cnptia.embrapa.br/handle/doc/514548
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Effects of experimental infections with larvae of Eustrongylides ignotus Jäegerskiold, 1909 and Contracaecum multipapillatum (Drasche, 1882) Baylis, 1920 in rabbits Arq. Bras. Med. Vet. Zootec.
Barros,L.A.; Tortelly,R.; Pinto,R.M.; Gomes,D.C..
Rabbits were infected per os with 10 Eustrongylides ignotus L4 and with 50 Contracaecum multipapillatum L3 per rabbit, recovered from naturally infected freshwater fishes (Hoplias malabaricus) in order to evaluate the patogenicity of these two nematode species in mammalian host. Two rabbits (20%) infected with E. ignotus died before the fourth day post-inoculation (one after 51 and the other after 78 hours). Six rabbits (60%) were inappetent until the fifth day following experimental inoculation. No clinical signs in rabbits inoculated with C. multipapillatum were observed; nevertheless, eight (80%) animals were positive for this nematode species. Rabbits inoculated with E. ignotus, had gastric congestion with hematoma of the gastric wall in 60% of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eustrongilydes ignotus; Contracaecum multipapillatum; Experimental infection; Rabbits; Pathology.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000300007
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Experimental herpes-like viral infections in marine bivalves: demonstration of interspecies transmission ArchiMer
Arzul, Isabelle; Renault, Tristan; Lipart, Cecile.
Since 1972, herpes-like virus infections have been reported in several marine bivalve species around the world. Viral detection was often associated with high mortality rates in larvae and spat. To determine whether a single virus is able to infect different bivalve host species, we carried out experimental transmission assays. As a first step, 8 assays were performed to infect axenic Crassostrea gigas larvae with virus from infected C. gigas larvae using a previously described protocol. The protocol appeared reliable and PCR was confirmed as a powerful technique for detecting viral DNA in experimentally infected oysters. The defined protocol was then applied to infect different bivalve species. Interspecies viral transmission was demonstrated under...
Tipo: Text Palavras-chave: Experimental infection; Host specificty; Interspecies transmission; Marine bivalves; Herpes like virus.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-819.pdf
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Experimental infection in Notodiaptomus sp. (Crustacea: Calanoida) with larvae of Camallanus sp. (Nematoda: Camallanidae) Arq. Bras. Med. Vet. Zootec.
Martins,M.L.; Ghiraldelli,L.; Garcia,F.; Onaka,E.M.; Fujimoto,R.Y..
This trial registered the experimental infection viability with nematode larvae Camallanus sp. in Notodiaptomus sp., a crustacean, which can be an intermediate host. Adult females of nematode were dissected from the intestines of Xiphophorus maculatus (Osteichthyes: Poeciliidae), at a fish farm in the State of São Paulo. Females were slightly compressed for larvae release, collected with Pasteur pipette and separated on Petri dishes with 9ml filtered water at 28.1ºC, from zooplankton culture. Treatments consisted of Petri dishes with 60 and 105 copepods, in which 120, 150 and 210 larvae of nematode were added in four replications. Twenty-four and 36h after exposition to the larvae, the copepods were fixed in 70% alcohol to record the amount of fixed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Experimental infection; Nematoda; Camallanus; Crustacea; Notodiaptomus.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200018
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Experimental infection of adult Scapharca broughtonii with Ostreid herpesvirus SB strain ArchiMer
Bai, Chang-ming; Wang, Qing-chen; Morga, Benjamin; Shi, Jie; Wang, Chong-ming.
We investigated the susceptibility of ark shell, Scapharca broughtonii, adults to Ostreid herpesvirus SB strain (OsHV-1-SB) through experimental infection by intramuscular injection assays. Results showed the onset of mortality occurred at 3 days post injection, one day after the water turbidity became evident in rearing tanks. The mortality curves for the challenged group were similar to those observed at affected hatcheries. Histological lesions, herpesvirus-like particles and high OsHV-1-SB quantities were detected in challenged ark shells. This is the first study to successfully reproduce OsHV-1 disease in Arcoida species, and very few studies in adult bivalves (over 24 months old).
Tipo: Text Palavras-chave: Scapharca broughtonii; Ostreid herpesvirus SB strain; OsHV-1-SB; Experimental infection; Viral quantity.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00360/47119/47081.pdf
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Experimental infection of dogs with Babesia microti OAK
Ohmori, T.; Uetsuka, K.; Nunoya, T.
Palavras-chave: Babesia microti; Dog; Experimental infection; Polymerase chain reaction; Indirect immunofluorescence test.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3132
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Experimental infection of horses with Vaccinia virus Ciência Rural
Barbosa,Claudio Henrique Gonçalves; Sant'Ana,Fabiano José Ferreira de; Cargnelutti,Juliana Felipetto; Flores,Eduardo Furtado; Teixeira Neto,Antônio Raphael; Santana,Rosália Bezerra de; Reis Junior,Janildo Ludolf.
ABSTRACT: Vaccinia virus (VACV) is the etiologic agent of bovine vaccinia, an emerging zoonotic disease with potential health issues for dairy herds and humans. VACV may occasionally infect other species, including horses. In this sense, an outbreak of VACV disease in horses was described in Pelotas, RS, in 2008, where a co-infection with two VACV strains (named Pelotas Virus 1 [P1V] and Pelotas Virus 2 [P2V]) was detected. Considering the rare occurrence of VACV infection in horses, the objective of this study was to investigate the susceptibility and pathogenesis of VACV infection in this species. Six adult horses were inoculated with VACV P1V or P2V (106.3TCID50/ml) through scarification of the nasolabial surface and monitored for virological and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: VACV; Experimental infection; Horses; Bovine vaccinia.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000300519
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Experimental infection with Brazilian Newcastle disease virus strain in pigeons and chickens BJM
Carrasco,Adriano de Oliveira Torres; Seki,Meire Christina; Benevenute,Jyan Lucas; Ikeda,Priscila; Pinto,Aramis Augusto.
Abstract This study was designed with the goal of adding as much information as possible about the role of pigeons (Columba livia) and chickens (Gallus gallus) in Newcastle disease virus epidemiology. These species were submitted to direct experimental infection with Newcastle disease virus to evaluate interspecies transmission and virus-host relationships. The results obtained in four experimental models were analyzed by hemagglutination inhibition and reverse transcriptase polymerase chain reaction for detection of virus shedding. These techniques revealed that both avian species, when previously immunized with a low pathogenic Newcastle disease virus strain (LaSota), developed high antibody titers that significantly reduced virus shedding after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gallus gallus; Columba livia; Experimental infection; RT-PCR; Serology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100231
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Experimental infection with different bacterial strains in larvae and juvenile Litopenaeus vannamei reared in Santa Catarina State, Brazil - doi: 10.4025/actascibiolsci.v32i3.5471 Biological Sciences
Buglione, Celso Carlos; UFSC; Vieira, Felipe do Nascimento; UFSC; Mouriño, José Luiz Pedreira; UFSC; Pedrotti, Fabiola Santiago; UFSC; Jatoba, Adolfo; UFSC; Martins, Maurício Laterça; UFSC.
This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7%...
Tipo: Resistencia a infecção Palavras-chave: 2.12.01.02-1 Shrimp; Experimental infection; Vibrio; Virulence; Immunology bacteriologia Shrimp; Experimental infection; Vibrio; Virulence; Immunology.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5471
Registros recuperados: 40
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