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| Registros recuperados: 37 | |
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| Medeiros,C.S.; Pontes-Filho,N.T.; Camara,C.A.; Lima-Filho,J.V.; Oliveira,P.C.; Lemos,S.A.; Leal,A.F.G.; Brandão,J.O.C.; Neves,R.P.. |
| The in vivo antifungal activity of the naphthoquinone beta-lapachone against disseminated infection by Cryptococcus neoformans was investigated. Swiss mice were immunosuppressed daily with dexamethasone (0.5 mg per mouse) intraperitoneally for 3 days, the procedure was repeated 4 days later, and the animals were then challenged intravenously with C. neoformans (10(6) CFU/mL) 1 week later. Seven days after infection, the mice were divided into groups and treated daily with beta-lapachone (10 mg/kg, iv) for 7 (N = 6) and 14 days (N = 10). Amphotericin B (0.5 mg/kg) was used as comparator drug and an additional group received PBS. Treatment with beta-lapachone cleared the yeast from the spleen and liver, and the fungal burden decreased approximately 10(4)... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Beta-lapachone; Cryptococcus neoformans; Cryptococcosis; Immunesuppression; Experimental infection; Amphotericin B. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400004 |
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| Eight-week old conventional female Swiss mice were inoculated intravenously with Yersinia enterocolitica O:3. A second group of normal mice was used as control. Five mice from each group were bled by heart puncture and their spleens were removed for spleen cell collection on the 3rd, 5th, 7th, 10th, 14th and 21st day after infection. Immunoglobulin-secreting spleen cells were detected by the isotype-specific protein A plaque assay. Total immunoglobulin levels were determined in mouse serum by single radial immunodiffusion and the presence of autoantibodies was determined by ELISA. We observed a marked increase in the total number of cells secreting immunoglobulins of all isotypes as early as on the 3rd day post-infection and the peak of secretion occurred... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Yersinia enterocolitica; Polyclonal activation; Experimental infection; Autoantibodies. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300015 |
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| Almeida,R.S.; Spilki,F.R.; Roehe,P.M.; Verinaud,L.M.C.; Arns,C.W.. |
| An immunoistochemical (IHC) test was developed to detect bovine respiratory syncytial virus (BRSV) in cell cultures and tissues of experimentally infected mice and calves, using a commercial monoclonal antibody (Mab) against human respiratory syncytial virus (HRSV), as a less expensive alternative, instead of producing specific monoclonal antibodies to BRSV. Clinical samples from calves suffering respiratory disease were also submitted to this test. IHC detected BRSV antigens in mouse tracheas (3, 5 and 7 days post-infection) and lungs (5 and 7 days post-infection), and in one of three lungs from experimentally infected calves. Lungs samples from two naturally infected calves were tested and resulted positive for BRSV by the IHC test. These results suggest... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine respiratory syncytial virus; Immunohistochemistry; Calf; Mice; Experimental infection; Monoclonal antibody. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600001 |
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| Morga, Benjamin; Renault, Tristan; Faury, Nicole; Lerond, Sophie; Garcia, Celine; Chollet, Bruno; Joly, Jean-pierre; Lapegue, Sylvie; Harrang, Estelle; Arzul, Isabelle. |
| Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Resistance; Experimental infection; Haemocytes; Gene expression; Apoptosis. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00405/51665/52213.pdf |
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| Petton, Bruno; Bruto, Maxime; James, Adele; Labreuche, Yannick; Alunno-bruscia, Marianne; Le Roux, Frederique. |
| Successive disease outbreaks in oyster (Crassostrea gigas) beds in France have resulted in dramatic losses in production, and subsequent decline in the oyster-farming industry. Deaths of juvenile oysters have been associated with the presence of a herpes virus (OsHV-1 µvar) and bacterial populations of the genus Vibrio. Although the pathogenicity of OsHV-1 µvar, as well as several strains of Vibrio has been demonstrated by experimental infections, our understanding of the complexity of infections occurring in the natural environment remains limited. In the present study, we use specific-pathogen-free (SPF) oysters infected in an estuarine environment to study the diversity and dynamics of cultured microbial populations during disease expression. We observe... |
| Tipo: Text |
Palavras-chave: Pacific oysters; Summer mortality; Herpes virus; Vibrio pathogenicity; Experimental infection. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00271/38220/36377.pdf |
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| SCHWARZ, D. G. G.; PIETRALONGA, P. A. G.; SOUZA, M. C. C.; CARVALHO, I. A.; CRUZEIRO, R. S.; MALAQUIAS, J. V.; BENJAMIM, L. A.; SILVA JÚNIOR, A.; MOREIRA, M. A. S.. |
| Resumo - Mycobacterium avium subespécie paratuberculosis (MAP) pode infectar ruminantes e permanecer subclínica por longos períodos nos rebanhos. A identificação de órgãos mais susceptíveis à infecção e a avaliação da expressão das citocinas no local da infecção são importantes para compreender a patogênese de MAP. Neste estudo foi avaliada a probabilidade de detecção de DNA de MAP e a expressão de citocinas em órgãos de camundongos C57BL/6 infectados por via intraperitoneal durante 120 dias. Dentre os órgãos avaliados, o baço (85%), cólon (75%) e fígado (60%) tiveram as maiores frequências de positividade. Quando comparadas essas frequências entre os órgãos, verificou-se que o baço teve 1,54 vezes mais probabilidade de ser positivo em relação ao íleo, e... |
| Tipo: Separatas |
Palavras-chave: Johne’s disease; Experimental infection; Doença de Johne; Murinos; Infecção experimental.; Mycobacterium avium; Mice.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036032 |
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| Langoni,H.; Das Dores,C. B.; Silva,R. C.; Pezerico,S. B.; Castro,A. P. B.; Da Silva,A. V.. |
| Detection of Toxoplasma gondii (T. gondii) DNA in blood can help to diagnose the disease in its acute phase; however, it must be considered that hemoglobin, present in blood, can inhibit polymerase activity, making impracticable the detection of DNA in samples. Mice were experimentally infected via oral route with ME49 and BTU2 strains cysts and RH strain tachyzoites; polymerase chain reaction was used to detect T. gondii DNA in mice sera 18, 24, 48, 96, and 192 hours post infection (PI). Toxoplama gondii DNA was detected in only one animal infected with BTU2 strain, genotype III (isolated from a dog with neurological signs) 18 hours PI. The agent's DNA was not detected in any sample of the other experimental groups. New studies must be carried out to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasmosis; PCR; Serum; Experimental infection; Mice. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200004 |
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| Arzul, Isabelle; Renault, Tristan; Lipart, Cecile. |
| Since 1972, herpes-like virus infections have been reported in several marine bivalve species around the world. Viral detection was often associated with high mortality rates in larvae and spat. To determine whether a single virus is able to infect different bivalve host species, we carried out experimental transmission assays. As a first step, 8 assays were performed to infect axenic Crassostrea gigas larvae with virus from infected C. gigas larvae using a previously described protocol. The protocol appeared reliable and PCR was confirmed as a powerful technique for detecting viral DNA in experimentally infected oysters. The defined protocol was then applied to infect different bivalve species. Interspecies viral transmission was demonstrated under... |
| Tipo: Text |
Palavras-chave: Experimental infection; Host specificty; Interspecies transmission; Marine bivalves; Herpes like virus. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-819.pdf |
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| Martins,M.L.; Ghiraldelli,L.; Garcia,F.; Onaka,E.M.; Fujimoto,R.Y.. |
| This trial registered the experimental infection viability with nematode larvae Camallanus sp. in Notodiaptomus sp., a crustacean, which can be an intermediate host. Adult females of nematode were dissected from the intestines of Xiphophorus maculatus (Osteichthyes: Poeciliidae), at a fish farm in the State of São Paulo. Females were slightly compressed for larvae release, collected with Pasteur pipette and separated on Petri dishes with 9ml filtered water at 28.1ºC, from zooplankton culture. Treatments consisted of Petri dishes with 60 and 105 copepods, in which 120, 150 and 210 larvae of nematode were added in four replications. Twenty-four and 36h after exposition to the larvae, the copepods were fixed in 70% alcohol to record the amount of fixed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Experimental infection; Nematoda; Camallanus; Crustacea; Notodiaptomus. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200018 |
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| Bai, Chang-ming; Wang, Qing-chen; Morga, Benjamin; Shi, Jie; Wang, Chong-ming. |
| We investigated the susceptibility of ark shell, Scapharca broughtonii, adults to Ostreid herpesvirus SB strain (OsHV-1-SB) through experimental infection by intramuscular injection assays. Results showed the onset of mortality occurred at 3 days post injection, one day after the water turbidity became evident in rearing tanks. The mortality curves for the challenged group were similar to those observed at affected hatcheries. Histological lesions, herpesvirus-like particles and high OsHV-1-SB quantities were detected in challenged ark shells. This is the first study to successfully reproduce OsHV-1 disease in Arcoida species, and very few studies in adult bivalves (over 24 months old). |
| Tipo: Text |
Palavras-chave: Scapharca broughtonii; Ostreid herpesvirus SB strain; OsHV-1-SB; Experimental infection; Viral quantity. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00360/47119/47081.pdf |
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| Barbosa,Claudio Henrique Gonçalves; Sant'Ana,Fabiano José Ferreira de; Cargnelutti,Juliana Felipetto; Flores,Eduardo Furtado; Teixeira Neto,Antônio Raphael; Santana,Rosália Bezerra de; Reis Junior,Janildo Ludolf. |
| ABSTRACT: Vaccinia virus (VACV) is the etiologic agent of bovine vaccinia, an emerging zoonotic disease with potential health issues for dairy herds and humans. VACV may occasionally infect other species, including horses. In this sense, an outbreak of VACV disease in horses was described in Pelotas, RS, in 2008, where a co-infection with two VACV strains (named Pelotas Virus 1 [P1V] and Pelotas Virus 2 [P2V]) was detected. Considering the rare occurrence of VACV infection in horses, the objective of this study was to investigate the susceptibility and pathogenesis of VACV infection in this species. Six adult horses were inoculated with VACV P1V or P2V (106.3TCID50/ml) through scarification of the nasolabial surface and monitored for virological and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: VACV; Experimental infection; Horses; Bovine vaccinia. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000300519 |
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| Buglione, Celso Carlos; UFSC; Vieira, Felipe do Nascimento; UFSC; Mouriño, José Luiz Pedreira; UFSC; Pedrotti, Fabiola Santiago; UFSC; Jatoba, Adolfo; UFSC; Martins, Maurício Laterça; UFSC. |
| This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7%... |
| Tipo: Resistencia a infecção |
Palavras-chave: 2.12.01.02-1 Shrimp; Experimental infection; Vibrio; Virulence; Immunology bacteriologia Shrimp; Experimental infection; Vibrio; Virulence; Immunology. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5471 |
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| Hoffmann,JL; Machado,JG; Gaio,FC; Dias-Melicio,LA; Langoni,H. |
| The immune response in leishmaniasis may result in a polarization of the T lymphocyte subpopulation, altering cell phenotype and resulting in immune protection or disease exacerbation. Leishmania may persist in the body either during asymptomatic infections or after treatment, which represents high risk under immunosuppression. The objective of this study was to evaluate the effect of infection with immunosuppression by dexamethasone associated with pentoxifylline on animal weight, spleen weight, spleen and hepatic parasitic load and immunopathology, as well as the IFN-γ and IL-10 production in spleen cell culture of Balb/c mice infected with Leishmania chagasi. The infection did not cause body weight gain in animals, but both the weight and size of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania chagasI; Immunosuppression; Cell-mediated response; Parasite burden; Experimental infection. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300004 |
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| Registros recuperados: 37 | |
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