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Antagonic effect of the inhibition of inducible nitric oxide on the mortality of mice acutely infected with Escherichia coli and Bacteroides fragilis BJMBR
Astolfi,R.S.; Khouri,D.G.; Brandizzi,L.I.V.; Ávila-Campos,M.J.; Andrade Jr.,H.F. de.
Sepsis, the leading cause of death in intensive care units, is associated with overproduction of nitric oxide (NO) due to inducible NO synthase (iNOS), responsible for some of the pathologic changes. Aminoguanidine (AG) is a selective iNOS inhibitor with reported inconsistent actions in sepsis. To investigate the influence of iNOS, we studied models of acute bacterial sepsis using acute challenges with aerobic (Escherichia coli) and anaerobic (Bacteroides fragilis) bacteria in the presence of AG. Six-week-old, 23 g, male and female BALB/c and C57Bl/6j mice, in equal proportions, were inoculated (ip) with bacteria in groups of 4 animals for each dose and each experiment in the absence or presence of AG (50 mg/kg, ip, starting 24 h before challenge and daily...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Nitric oxide; Sepsis; Experimental model; Bacterial infections; Escherichia coli; Bacteroides fragilis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300006
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Characterization of the virulence, growth temperature and antibiotic resistance of the Campylobacter jejuni IAL 2383 strain isolated from humans BJM
Fonseca,B.B.; Rossi,D.A.; Maia,C.A.; Nalevaiko,P.C.; Melo,R.T.; Cuccato,L.P.; Beletti,M.E..
The objective of this study was to characterize the C. jejuni IAL2383 strain isolated from humans in Brazil. Transcripts for the racR, dnaJ and ciaB genes were found and flaA, plda and cadF genes were present in the genome and bacteria was sensitive to most of the important antimicrobials used to treat humans. C. jejuni IAL2383 is a good experimental model to analyze the interactions with cells.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Virulence; Experimental model; Thermotolerance; Campylobacter jejuni.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100039
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Development of a BALB/c mouse model of Helicobacter pylori infection with fresh and frozen bacteria Biol. Res.
Rabelo-Gonçalves,Elizabeth M A; Nishimura,Nancy F; Murilo R Zeitune,José.
An experimental model for H. pylori infection was established by intragastrically challenging BALB/c mice with 1 ml (10(8) CFU/ml) of suspension for two consecutive days. Animals were divided into three groups. GA: mice inoculated with fresh bacteria; GB: mice inoculated with frozen bacteria, and GC: mice inoculated with brucella broth (control group). Animals were killed at 7, 14, 21, 28, 35 and 60 days pi and fragments of stomach and duodenum were collected, paraffin embedded and stained by hematoxylin-eosin and Giemsa. The results showed that challenged mice exhibited mild duodenitis and gastritis. In group GA, infiltration in the duodenum was lymphoplasmacytic until day 35; in group GB, it was lymphomonocytic for 60 days pi. In the stomach, H. pylori...
Tipo: Journal article Palavras-chave: BALB/c mouse; Experimental model; Helicobacter pylori.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100012
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Development of Adrenal Glands from the Embryonic Phase to Puberty in Spix’s Yellow-toothed Cavies (Galea spixii, Wagler, 1831) BABT
Lobo,Luis Miguel; Santos,Amílton Cesar dos; Oliveira,Moacir Franco de; Assis-Neto,Antônio Chaves.
ABSTRACT Several authors have underscored the importance of establishing parameters in morphological development by gender comparison to establish clinical and pre-clinical assays through the use of experimental models. Current research compares the morphometry of right and left adrenal glands of males and females and describes differentiation of the cortex and medulla tissue during the embryonic, pre-puberty and puberty phases in Spix´s yellow-toothed cavies. Embryos aged 22 (22D), 25 (25D), 30 (30D), 40 (44D) and >50 (50D) days of gestation and neonates aged 15 (15DPN) (DPN= Days postnatal), 30 (30DPN) and 90 (90DPN) days after birth were analyzed. Comparisons included morphometric and histological analysis in all periods described. When compared the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adrenal córtex; Cavidae; Experimental model; Rodentia; Steroidogenesis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100444
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Experimental evidence of dietary ciguatoxin accumulation in an herbivorous coral reef fish ArchiMer
Clausing, Rachel J.; Losen, Barbara; Oberhaensli, Francois R.; Darius, H. Taiana; Sibat, Manoella; Hess, Philipp; Swarzenski, Peter W.; Chinain, Mireille; Bottein, Marie-yasmine Dechraoui.
Ciguatoxins (CTXs) are potent algal toxins that cause widespread ciguatera poisoning and are found ubiquitously in coral reef food webs. Here we developed an environmentally-relevant, experimental model of CTX trophic transfer involving dietary exposure of herbivorous fish to the CTX-producing microalgae Gambierdiscus polynesiensis. Juvenile Naso brevirostris were fed a gel-food embedded with microalgae for 16 weeks (89 cells g.1 fish daily, 0.4 ¦Ìg CTX3C equiv kg.1 fish). CTXs in muscle tissue were detectable after 2 weeks at levels above the threshold for human intoxication (1.2 ¡À 0.2 ¦Ìg CTX3C equiv kg.1). Although tissue CTX concentrations stabilized after 8 weeks (¡«3 ¡À 0.5 ¦Ìg CTX3C equiv kg.1), muscle toxin burden (total ¦Ìg CTX in muscle tissue)...
Tipo: Text Palavras-chave: Ciguatoxin; Bioaccumulation; Growth dilution; Trophic transfer; Herbivorous fish; Experimental model.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00442/55339/60476.pdf
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Experimental myocardial hypertrophy induced by a minimally invasive ascending aorta coarctation BJMBR
Martins,A.S.; Aguilera,N.W.; Matsubara,B.B.; Bregagnollo,E.A..
Ascending aorta coarctation was produced by a minimally invasive technique in rabbits. Animal mortality was 5%. Morphometric and hemodynamic parameters were evaluated. A parabiotically isolated heart model was used to assess the hemodynamic parameters. Left ventricular weight/body weight ratio and muscle area showed clear evidence of hypertrophy when compared to control. The hemodynamic changes in the isolated heart model suggested decreased diastolic and systolic function in the coarcted group. The present model produced hypertrophy with low mortality rates as a result of its less invasive nature.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Ventricular hypertrophy; Ventricular function; Experimental model.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300017
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From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studies. Repositório Alice
JORJÃO, A. L.; OLIVEIRA, L. D.; SCORZONI, L.; FIGUEIREDO-GODOI, L. M. A.; PRATA, M. C. de A.; JORGE, A. O. C.; JUNQUEIRA, J. C..
ABSTRACT Galleria mellonella is a well-accepted insect model for the study of pathogen-host interactions and antimicrobial compounds. The main advantages of this model include the low cost of maintenance, the fast life cycle, the possibility of using a large number of caterpillars and the innate immune system, which is evolutionarily conserved relative to mammals. Because of these advantages, different research groups have been working to implement the rearing of G. mellonella in laboratory conditions. This protocol describes our experience in the rearing of G. mellonella caterpillars for experimental infection models and the influence of different artificial diets on developmental and physiological parameters. Here, we suggest a diet composition that...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Experimental model; In vivo study; Galleria mellonella; Rearing.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094672
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Infection dynamics of a V. splendidus strain pathogenic to Mytilus edulis : In vivo and in vitro interactions with hemocytes ArchiMer
Ben Cheikh, Yosra; Travers, Marie-agnes; Le Foll, Frank.
The pathogenic strain V. splendidus 10/068 1T1 has previously been reported for its virulence to the blue mussel and for its capacity to alter immune responses. In this study, we expanded the knowledge on hemocyte-pathogen interactions by using in vitro and in vivo assays. V. splendidus 10/068 1T1 severely inhibited cell adhesion and acidic vacuole formation unlike the innocuous phylogenetically related V. splendidus 12/056 M24T1 which had no effect on these cell functions. Furthermore, the virulent bacteria decreased hemocyte viability (59% of viability after 24 h). Infection dynamics were explored by using a model based on water tank cohabitation with septic mussels infected by GFP-tagged V. splendidus 10/068 1T1. Experimental infections were...
Tipo: Text Palavras-chave: Innate immunity; Bivalves; Vibrio; Infection; Experimental model; Hemocyte.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00400/51136/53772.pdf
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Influence of transcutaneous electrical stimulation on heterotopic ossification: an experimental study in Wistar rats BJMBR
Zotz,T.G.G.; Paula,J.B. de.
Heterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues, resulting in joint mobility deficit and pain. Different treatment modalities have been tried to prevent HO development, but there is no consensus on a therapeutic approach. Since electrical stimulation is a widely used resource in physiotherapy practice to stimulate joint mobility, with analgesic and anti-inflammatory effects, its usefulness for HO treatment was investigated. We aimed to identify the influence of electrical stimulation on induced HO in Wistar rats. Thirty-six male rats (350-390 g) were used, and all animals were anesthetized for blood sampling before HO induction, to quantify the serum alkaline phosphatase. HO induction...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heterotopic ossification; Experimental model; Electrical stimulation; Functional electrical stimulation; Transcutaneous electrical nerve stimulation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101055
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Maternal administration of cannabidiol promotes an anti-inflammatory effect on the intestinal wall in a gastroschisis rat model BJMBR
Callejas,G.H.; Figueira,R.L.; Gonçalves,F.L.L.; Volpe,F.A.P.; Zuardi,A.W.; Crippa,J.A.; Hallak,J.E.; Sbragia,L..
Gastroschisis (GS) is an abdominal wall defect that results in histological and morphological changes leading to intestinal motility perturbation and impaired absorption of nutrients. Due to its anti-inflammatory, antioxidant, and neuroprotective effects, cannabidiol (CBD) has been used as a therapeutic agent in many diseases. Our aim was to test the effect of maternal CBD in the intestine of an experimental model of GS. Pregnant rats were treated over 3 days with CBD (30 mg/kg) after the surgical induction of GS (day 18.5 of gestation) and compared to controls. Fetuses were divided into 4 groups: 1) control (C); 2) C+CBD (CCBD); 3) gastroschisis (G), and 4) G+CBD (GCBD). On day 21.5 of gestation, the fetuses were harvested and evaluated for: a) body...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gastroschisis; Experimental model; Prenatal treatment; Cannabidiol.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500607
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Oral infection of neonate gerbils by Neospora caninum tachyzoites Ciência Rural
Ferreira,Maiara Sanitá Tafner; Vogel,Fernanda Silveira Flores; Sangioni,Luis Antonio; Weber,Augusto; Bräunig,Patricia; Vaz,Marcos André Braz; Cezar,Alfredo Skrebsky.
ABSTRACT: Neosporosis is a parasitic disease caused by the protozoan Neospora caninum which results in major economic losses for cattle breeding due to abortion and other reproductive disorders. Gerbils (Meriones unguiculatus) are commonly used as experimental models for neosporosis due to their high susceptibility to N. caninum infection, both by oocysts ingestion as by tachyzoites/bradyzoites parenteral inoculation. However, the risk of transmission by tachyzoites ingestion is not fully elucidated. In this study, infection of neonate gerbils by N. caninum (NC-1 strain) tachyzoites inoculated by the oral route and the parasite distribution in gerbils' tissues were evaluated by protozoan DNA detection. Seventeen neonate gerbils, aged 4-5 days, were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular diagnosis; Experimental model; Bovine neosporosis; Rodents.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400654
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Paraquat poisoning: an experimental model of dose-dependent acute lung injury due to surfactant dysfunction BJMBR
Silva,M.F.R.; Saldiva,P.H.N..
Since the most characteristic feature of paraquat poisoning is lung damage, a prospective controlled study was performed on excised rat lungs in order to estimate the intensity of lesion after different doses. Twenty-five male, 2-3-month-old non-SPF Wistar rats, divided into 5 groups, received paraquat dichloride in a single intraperitoneal injection (0, 1, 5, 25, or 50 mg/kg body weight) 24 h before the experiment. Static pressure-volume (PV) curves were performed in air- and saline-filled lungs; an estimator of surface tension and tissue works was computed by integrating the area of both curves and reported as work/ml of volume displacement. Paraquat induced a dose-dependent increase of inspiratory surface tension work that reached a significant two-fold...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paraquat; Experimental model; Acute lung injury; Pressure-volume curve; Respiratory work.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300018
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Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet International Journal of Morphology
Neves,Renata Heisler; Barros Alencar,Alba Cristina Miranda de; Aguila,Marcia Barbosa; Mandarim-de-Lacerda,Carlos Alberto; Machado-Silva,José Roberto; Gomes,Delir Corrêa.
This study evaluated whether a high fat diet (HFC group) induces overweight, hepatic steatosis and plasma lipoproteins level alteration compared to standard chow diet (SC group). Female mice were submitted to each diet over 6 months. Body mass and food intake were evaluated weekly throughout the experiment. Total cholesterol, TG, LDL-c, HDL-c and VLDL-c were analyzed. Mice were sacrificed to remove liver, spleen, heart and intestine. The volume of the organs was determined according to the submersion method. Fixed livers were embedded in paraffin and stained with hematoxylin and eosin and Masson's trichrome. The analysis used a video microscope system and a test-system with 42 test-points. The volume density was estimated for hepatocytes, steatosis and...
Tipo: Journal article Palavras-chave: Hypercholesterolemia; Experimental model; Mice; Cholesterol; Diet; Liver.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500018
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Walker-256 Tumor: Experimental Model, Implantation Sites and Number of Cells for Ascitic and Solid Tumor Development BABT
Amaral,Luane Aparecida do; Souza,Gabriel Henrique Oliveira de; Santos,Mirelly Romeiro; Said,Yasmin Lany Ventura; Souza,Bruna Brandão de; Oliveira,Rodrigo Juliano; Santos,Elisvania Freitas dos.
Abstract The Walker-256 tumor is an important experimental model that allow the development of therapies as the biological behavior of this tumor is similar that occur in humans. In front of the above considerations, the aim of this study was to describe the experimental model of Walker-256 tumor, identify the implantations sites as well as define a usual quantity of tumoral cells to induce the ascitic and solid tumor, according to the specialized literature. Were selected 45 articles using the keyword “Walker-256 tumor”, free available. Were possible to observe that 58% (n=26) of the studies inoculate the tumor cells in the animals flank 33% (n = 15) in the tibia bone, 7% (n = 3) in the femur and 2% (n = 1) in the paw. The major quantitates of cells used...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Walker-256 tumor; Cancer; Experimental model; Rats.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100900
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