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Registros recuperados: 78
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A karyotypic study of three southern Brazilian Asteraceae species using fluorescence in situ hybridization with a 45S rDNA probe and C-CMA3 banding Genet. Mol. Biol.
Fregonezi,Jéferson N.; Torezan,José M.D.; Vanzela,André L.L..
The Asteraceae, one of the largest families of flowering plants, contains about 1,100 genera and 20,000 species, and is well known for its extensive karyotypic variation. In this study, conventional Feulgen staining, C-CMA3 banding, and fluorescence in situ hybridization with a 45S rDNA probe were used to determine the chromosome number and the number and physical position of GC-rich heterochromatin and 45S rDNA sites in three Asteraceae weed species (Crepis japonica, Galinsoga parviflora and Chaptalia nutans). The three species exhibited karyotype differences in the chromosome number and shape, as a commom feature of Asteraceae. However, the 45S rDNA sites always occurred on the short chromosomal arms, associated with GC-heterochromatin. Althought of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CMA3; FISH; Heterochromatin; Polyploidy; 45S rDNA.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200016
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A pericentric inversion of chromosome 4 in pigs Inra
Ducos, A.; Pinton, A.; Séguéla, A.; Berland, H.M.; Blanc, M.F.; Darré, A.; Pinto, P.; Yerle, M.; Darré, R..
Le principal centre français d’insémination artificielle porcine a récemment décidé de procéder à un contrôle chromosomique systématique de tous les verrats de races pures. Cette pratique a permis de mettre en évidence, chez quatre verrats Large White issus de quatre élevages de sélection différents, une nouvelle inversion péricentrique impliquant le chromosome 4. L’examen des chromosomes colorés en bandes GTG a révélé une double cassure, la première au niveau de la bande claire 4q2.3 du bras long, la seconde en limite supérieure de la bande sombre 4p1.4 sur le bras court, suivie d’une inversion de la partie péricentrique du chromosome. Cette anomalie peut être décrite, conformément à la nomenclature en vigueur, de la façon suivante :...
Tipo: Journal Article Palavras-chave: ANOMALIE CHROMOSOMIQUE; INVERSION PERICENTRIQUE; PERFORMANCES DE REPRODUCTION; FISH.
Ano: 1997 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20076a4b47f9&uri=/notices/prodinra1/2007/09/
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A study of the constitutive heterochromatin and nucleolus organizer regions of Isocopris inhiata and Diabroctis mimas (Coleoptera: Scarabaeidae, Scarabaeinae) using C-banding, AgNO3 staining and FISH techniques Genet. Mol. Biol.
Bione,Edgar; Camparoto,Marjori Leiva; Simões,Zilá Luz Paulino.
Meiotic and mitotic chromosomes of Isocopris inhiata and Diabroctis mimas were studied by standard staining procedures, C-banding, silver nitrate staining and FISH using Apis mellifera 28S rDNA as probe. Isocopris inhiata presented a 2n = 18 (8II+ Xy p) karyotype, composed of meta-submetacentric chromosomes with gradual reduction in size. The karyotype of D. mimas was 2n = 20 (9II+ Xy p), composed of meta-submetacentric (pairs 1, 2, 3, 4 and 7) and acrocentric (pairs 5, 6, 8 and 9) chromosomes, with gradual reduction in size. Analysis of constitutive heterochromatin revealed similar C-banding patterns for both species, showing pericentromeric and telomeric bands and diphasic chromosomes. In addition, the X chromosomes of these species were found to be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coleoptera; Heterochromatin; NORs; FISH.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100019
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Abundance and diversity of Planctomycetes in a Tyrrhenian coastal system of central Italy ArchiMer
Pizzetti, Ilaria; Gobet, Angelique; Fuchs, Bernhard M.; Amann, Rudolf; Fazi, Stefano.
The phylum Planctomycetes is involved in important processes, such as the mineralization of algal biomass and the removal of nitrogen. Using a combination of 16S rRNA sequence analysis and in situ hybridization, we analyzed the diversity and dynamics of Planctomycetes in a shallow meso-eutrophic lake, Lago di Paola, Italy. Planctomycetes detected by the probe PLA46 accounted for 1 to 5% of prokaryotic picoplankton. Abundances were higher in the coastal lake than in the adjacent marine waters. In the surface waters of the lake, the numbers of Planctomycetes fluctuated greatly, reaching a maximum of 1.5 x 10(6) cells ml(-1) in July. The hypoxic bottom waters had less variable cell abundances. The Planctomycetes counts were positively correlated with...
Tipo: Text Palavras-chave: Coastal lake ecology; Planctomycetes; Bacterial diversity; FISH; CARD-FISH; 16S rRNA gene sequencing.
Ano: 2011 URL: https://archimer.ifremer.fr/doc/00488/59948/63196.pdf
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Acute promyelocytic leukemia: the study of t(15;17) translocation by fluorescent in situ hybridization, reverse transcriptase-polymerase chain reaction and cytogenetic techniques BJMBR
Chauffaille,M.L.L.F.; Figueiredo,M.S.; Beltrani,R.; Antunes,S.V.; Yamamoto,M.; Kerbauy,J..
Acute promyelocytic leukemia (AML M3) is a well-defined subtype of leukemia with specific and peculiar characteristics. Immediate identification of t(15;17) or the PML/RARA gene rearrangement is fundamental for treatment. The objective of the present study was to compare fluorescent in situ hybridization (FISH), reverse transcriptase-polymerase chain reaction (RT-PCR) and karyotyping in 18 samples (12 at diagnosis and 6 after treatment) from 13 AML M3 patients. Bone marrow samples were submitted to karyotype G-banding, FISH and RT-PCR. At diagnosis, cytogenetics was successful in 10 of 12 samples, 8 with t(15;17) and 2 without. FISH was positive in 11/12 cases (one had no cells for analysis) and positivity varied from 25 to 93% (mean: 56%). RT-PCR was done...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute promyelocytic leukemia; Karyotype; FISH; RT-PCR; PML/RARA genem rearrangement.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600006
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Adaptação da metodologia de 'BAC-FISH' de alta resolução para estudos genômicos comparativos em Musa. Repositório Alice
FALCÃO, R..
Bananeiras são plantas cultivadas principalmente em países em desenvolvimento das regiões tropicais e subtropicais, onde a produção de bananas tem grande impacto econômico e social, pois envolve um número muito grande de pequenos produtores. A maioria dos genótipos de banana utilizados na alimentação é de plantas diplóides, triplóides e tetraplóides, oriundos de cruzamentos entre Musa acuminata Colla e Musa balbisiana Colla. O caráter semiperene e a propagação assexuada de bananeiras facilitam a transmissão e/ou disseminação de doenças, além de impedir a chance de recornbinação gênica, processo fundamental para o melhoramento genético, o qual possui, dentre muitos objetivos, a obtenção de plantas resistentes a estresses bióticos e abióticos. A utilização...
Tipo: Tese/dissertação (ALICE) Palavras-chave: Musa acuminata; Musa balbisiana; Paquíteno; Microsporogênese; BAC; FISH.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/662241
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Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences Neotropical Ichthyology
Barreto,Silvia B.; Cioffi,Marcelo B.; Medrado,Aline S.; Silva,André T.; Affonso,Paulo R. A. M.; Diniz,Débora.
ABSTRACT Characiformes is the most cytogenetically studied group of freshwater Actinopterygii, but karyotypical data of several taxa remain unknown. This is the case of Nematocharax , regarded as a monotypic genus and characterized by marked sexual dimorphism. Therefore, we provide the first cytogenetic report of allopatric populations of Nematocharax venustus based on distinct methods of chromosomal banding and fluorescence in situ hybridization (FISH) with repetitive DNA probes (18S and 5S rDNA). The karyotype macrostructure was conserved in all specimens and populations, independently on sex, since they shared a diploid number (2n) of 50 chromosomes divided into 8m+26sm+14st+2a. The heterochromatin was mainly distributed at pericentromeric regions and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 18S rDNA; 5S rDNA; Coastal drainages; FISH; Population cytogenetics.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200205
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An in vivo and in vitro assessment of TOR signaling cascade in rainbow trout (Oncorhynchus mykiss) Inra
Seiliez, I.; Gabillard, J.C.; Skiba-Cassy, S.; Garcia-Serrana, D.; Gutierrez, J.; Kaushik, S.; Panserat, S.; Tesseraud, S..
Tipo: Journal Article Palavras-chave: FEEDING; SIGNALING; PROTEIN SYNTHESIS; FISH.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008626f838b&uri=/notices/prodinra1/2010/09/
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B chromosome and NORs polymorphism in Callichthys callichthys (Linnaeus, 1758) (Siluriformes: Callichthyidae) from upper Paraná River, Brazil Neotropical Ichthyology
Konerat,Jocicléia Thums; Bueno,Vanessa; Baumgartner,Lucas; Martins-Santos,Isabel Cristina; Margarido,Vladimir Pavan.
B chromosomes are extra chromosomes from the normal chromosomal set, found in different organisms, highlighting their presence on the group of fishes. Callichthys callichthys from the upper Paraná River has a diploid number of 56 chromosomes (26 m-sm + 30 st-a) for both sexes, with the presence of a sporadically acrocentric B chromosome. Moreover, one individual presented a diploid number of 57 chromosomes, with the presence of a morphologically ill-defined acrocentric B chromosome in all analyzed cells. The physical mapping of 5S and 18S rDNA shows multiple 5S rDNA sites and only one pair of chromosomes with 18S sites in C. callichthys, except for two individuals. These two individuals presented a third chromosome bearing NORs (Ag-staining and 18S rDNA)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 18S rDNA; 5S rDNA; Chromosomal rearrangements; FISH; Supernumerary chromosome.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000300603
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Biodiversity and quantification of functional bacteria in deammonification process. Repositório Alice
PRÁ, M. C. de; KUNZ, A.; BORTOLI, M.; VIANCELLI, A.; SCUSSIATO, L. A.; SOARES, H. M..
2015
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: ANAMMOX; Ammonia oxidizing bacteria; Partial nitritation; FISH; QPCR.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1045554
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Chromogenic in situ hybridization compared with other approaches to evaluate HER2/neu status in breast carcinomas BJMBR
Rosa,F.E.; Santos,R.M.; Rogatto,S.R.; Domingues,M.A.C..
Human epidermal growth factor receptor 2 (HER2) has been evaluated in breast cancer patients to identify those most likely to benefit from herceptin-targeted therapy. HER2 amplification, detected in 20-30% of invasive breast tumors, is associated with reduced survival and metastasis. The most frequently used technique for evaluating HER2 protein status as a routine procedure is immunohistochemistry (IHC). HER2 copy number alterations have also been evaluated by fluorescence in situ hybridization (FISH) in moderate immunoexpression (IHC 2+) cases. An alternative procedure to evaluate gene amplification is chromogenic in situhybridization (CISH), which has some advantages over FISH, including the correlation between HER2 status and morphological features....
Tipo: Info:eu-repo/semantics/article Palavras-chave: CISH; Breast cancer; HER2; Immunohistochemistry; FISH.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300207
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Chromosomal analyses in Megalonema platanum (Siluriformes: Pimelodidae), an endangered species from South American rivers Neotropical Ichthyology
Carvalho,Rafael Augusto de; Sanchez,Sebástian; Swarça,Ana Claudia; Fenocchio,Alberto Sergio; Martins-Santos,Isabel C.; Dias,Ana Lúcia.
This study presents chromosomal data of Megalonema platanum from rio Tibagi, Paraná, Brazil and from rio Paraná, Argentina. The diploid number was equal 54 with karyotype composition of 24m+16sm+2st+12a in both populations. The AgNOR sites were detected in the terminal position of a submetacentric pair of the two analyzed populations, coinciding with secondary constrictions on the short arm of pair 15. CMA3 and FISH with 18S rDNA probe displayed fluorescent signals that correspond to the AgNOR sites and secondary constriction. The presence of a small acrocentric supernumerary chromosome can be observed in M. platanum from rio Tibagi, with centromeric heterochromatin. Others heterochromatic blocks were evidenced in the terminal position of some chromosome...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; C-banding; CMA3; FISH; NORs; Pimelodidae.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000100017
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Chromosomal distribution and evolution of abundant retrotransposons in plants: gypsy elements in diploid and polyploid Brachiaria forage grasses. Repositório Alice
SANTOS, F. C.; VALLE, C. B. do; CHIARI, L..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Centromeres; Retrotransposons; FISH; In situ hybridization; Metaviridae; Grasses; Genomics; Genome organization; Transposons; Transposable elements; Genetics; Repetitive DNA; Chromosomes.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1040145
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Chromosomal diversification of diploid number, heterochromatin and rDNAs in two species of Phanaeus beetles (Scarabaeidae, Scarabaeinae) Genet. Mol. Biol.
Arcanjo,Amanda; Cabral-de-Mello,Diogo Cavalcanti; Martins,Cesar; Moura,Rita de Cássia de; Souza,Maria José de.
The genus Phanaeus is included in the tribe Phanaeini, one of the most diverse tribes within the subfamily Scarabaeinae in terms of chromosomal characteristics. However, so far the species of this genus were not studied with differential cytogenetic techniques, limiting any inference of the probable mechanisms responsible for this diversity. In this work, several techniques were applied with the aim of cytogenetically characterizing two Phanaeus species. The karyotype found for Phanaeus (Notiophanaeus) chalcomelas was 2n = 12, neo-XY, and that of P. (N.) splendidulus was 2n = 20, Xy p, considered primitive for the family Scarabaeidae. The chromosomes of both species showed a high amount of constitutive heterochromatin (CH), with blocks rich in base pairs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotype; Chromosome evolution; Repetitive DNA; FISH.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300007
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Chromosomal diversity in three species of electric fish (Apteronotidae, Gymnotiformes) from the Amazon Basin Genet. Mol. Biol.
Silva,Fernando Henrique Ramos; Pieczarka,Julio Cesar; Cardoso,Adauto Lima; Silva,Patrícia Corrêa da; Oliveira,Jonas Alves de; Nagamachi,Cleusa Yoshiko.
Cytogenetic studies were carried out on samples of Parapteronotus hasemani, Sternarchogiton preto and Sternarchorhamphus muelleri (Apteronotidae, Gymnotiformes) from the Amazon basin. The first two species exhibited both a 2n = 52 karyotype, but differed in their karyotypic formulae, distribution of constitutive heterochromatin, and chromosomal location of the NOR. The third species, Sternarchorhamphus muelleri, was found to have a 2n = 32 karyotype. In all three species the DAPI and chromomycin A3 staining results were consistent with the C-banding results and nucleolar organizer region (NOR) localization. The 18S rDNA probe confirmed that there was only one pair of ribosomal DNA cistron bearers per species. The telomeric probe did not reveal interstitial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fluorochromes; FISH; Chromosomal rearrangements; Biodiversity.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500006
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Chromosomal localization of the 18S-28S and 5S rRNA genes and (TTAGGG)n sequences of butterfly lizards (Leiolepis belliana belliana and Leiolepis boehmei, Agamidae, Squamata) Genet. Mol. Biol.
Srikulnath,Kornsorn; Uno,Yoshinobu; Matsubara,Kazumi; Thongpan,Amara; Suputtitada,Saowanee; Apisitwanich,Somsak; Nishida,Chizuko; Matsuda,Yoichi.
Chromosomal mapping of the butterfly lizards Leiolepis belliana belliana and L. boehmei was done using the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences. The karyotype of L. b. belliana was 2n = 36, whereas that of L. boehmei was 2n = 34. The 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, while the 5S rRNA genes were found in the pericentromeric region of chromosome 6 in both species. Hybridization signals for the (TTAGGG)n sequence were observed at the telomeric ends of all chromosomes, as well as interstitially at the same position as the 18S-28S rRNA genes in L. boehmei. This finding suggests that in L. boehmei telomere-to-telomere fusion probably occurred between chromosome 1 and a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosomal mapping; FISH; Interstitial telomeric site; Leiolepidinae; Ribosomal RNA gene; Telomeric sequence.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400009
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Chromosomal organization and phylogenetic relationships in Hypochaeris species (Asteraceae) from Brazil Genet. Mol. Biol.
Ruas,Claudete de Fátima; Vanzela,André L.L.; Santos,Melissa O.; Fregonezi,Jeferson N.; Ruas,Paulo Maurício; Matzenbacher,Nelson I.; Aguiar-Perecin,Margarida L.R. de.
The association of cytogenetic and molecular techniques has contributed to the analysis of chromosome organization and phylogeny in plants. The fluorochrome GC-specific CMA3, fluorescent in situ hybridization (FISH) and RAPD (Random Amplified Polymorphic DNA) markers were used to investigate chromosome structure and genetic relationships in Hypochaeris (Asteraceae). Seven species native to South America, and two species introduced from Europe (H. glabra and Hypochaeris sp) were studied. FISH with rDNA probes identified one or two loci of 18S-5.8S-25S rDNA in the South American Hypochaeris species and one locus in the European species. Only one 5S rDNA locus was seen in all species studied. Blocks of GC-rich heterochromatin (CMA-positive bands) associated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome banding; FISH; Hypochaeris; Phylogenetic relationship; RAPD.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100023
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Chromosomal polymorphism and comparative chromosome painting in the rufous-collared sparrow (Zonotrichia capensis) Genet. Mol. Biol.
Bülau,Sandra Eloisa; Kretschmer,Rafael; Gunski,Ricardo José; Garnero,Analía del Valle; O’Brien,Patricia C. M.; Ferguson-Smith,Malcolm A.; Oliveira,Edivaldo Herculano Correa de; Freitas,Thales Renato Ochotorena de.
Abstract Zonotrichia capensis is widely distributed in the Neotropics. Previous cytogenetic studies demonstrated the presence of polymorphisms in two chromosome pairs (ZCA2 and ZCA4). Here, we report results based on comparative chromosome painting, using probes derived from Gallus gallus and Leucopternis albicollis, focused on characterizing the chromosome organization of Z. capensis. Our results demonstrate the conservation of ancestral syntenies as observed previously in other species of passerine. Syntenies were rearranged by a series of inversions in the second chromosome as described in other Passeriformes, but in this species, by using probes derived from L. albicollis we observed an extra inversion in the second chromosome that had not previously...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Birds; Chromosomal rearrangements; Molecular cytogenetics; FISH.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500799
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Chromosome mapping of ribosomal genes and histone H4 in the genus Radacridium (Romaleidae) Genet. Mol. Biol.
Anjos,Allison; Loreto,Vilma; Souza,Maria José de.
In this study, two species of Romaleidae grasshoppers, Radacridium mariajoseae and R.nordestinum, were analyzed after CMA3/DA/DAPI sequential staining and fluorescence in situ hybridization (FISH) to determine the location of the 18S and 5S rDNA and histone H4 genes. Both species presented karyotypes composed of 2n = 23, X0 with exclusively acrocentric chromosomes. CMA3+ blocks were detected after CMA3/DA/DAPI staining in only one medium size autosome bivalent and in the X chromosome in R. mariajoseae. On the other hand, all chromosomes, except the L1 bivalent, of R. nordestinum presented CMA3+ blocks. FISH analysis showed that the 18S genes are restricted to the X chromosome in R. mariajoseae, whereas these genes were located in the L2,S9 and S10...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; FISH; Fluorochromes; Grasshoppers; RDNA.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300006
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Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas Genet. Mol. Biol.
Veiga,Luciana CS; Bérgamo,Nádia A; Kowalski,Luiz Paulo; Rogatto,Silvia R.
Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The most frequent structural alteration was del(22)(q13.1). As compared to G-banding, fluorescence in situ hybridization (FISH) proved to be an effective technique for detecting aneuploidy. Interphase FISH with a chromosome 17 centromere probe disclosed a high frequency of monosomy for chromosome 17, in contrast with G-banding, by which clonal monosomy 17 was detected in only three of the tumors. Painting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: FISH; Chromosomal aberrations; Head and neck cancer; Chromosome 17.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200003
Registros recuperados: 78
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