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Registros recuperados: 23
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A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.) Electron. J. Biotechnol.
Barkley,Noelle A; Wang,Ming Li; Pittman,Roy N.
The high oleic (C18:1) phenotype in peanuts has been previously demonstrated to result from a homozygous recessive genotype (ol1ol1ol2ol2) in two homeologous fatty acid desaturase genes (FAD2A and FAD2B) with two key SNPs. These mutant SNPs, specifically G448A in FAD2A and 442insA in FAD2B, significantly limit the normal function of the desaturase enzyme activity which converts oleic acid into linoleic acid by the addition of a second double bond in the hydrocarbon chain. Previously, a genotyping assay was developed to detect wild type and mutant alleles in FAD2B. A real-time PCR assay has now been developed to detect wild type and mutant alleles (G448A) in FAD2A using either seed or leaf tissue. This assay was demonstrated to be applicable for the...
Tipo: Journal article Palavras-chave: Fatty acid composition; Gas chromatography; Peanut (Arachis hypogaea L.); Real-time PCR; SNP genotyping.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100009
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Body composition and sensory quality of wild and farmed brown-trout (Salmo trutta) and of farmed rainbow-trout (Oncorhynchus mykiss) Ciência Rural
Antão-Geraldes,Ana Maria; Hungulo,Sílvia Rafael; Pereira,Etelvina; Teixeira,Amílcar; Teixeira,Alfredo; Rodrigues,Sandra.
ABSTRACT: Body composition (total crude protein, lipid, ash, dry matter and moisture) and fatty acid profiles were compared between wild and farmed brown trout and between farmed rainbow trout. Farmed brown trout contained the highest amount of crude protein (18.39%), whereas farmed rainbow trout contained higher levels of crude lipid (2.35%). Thirty six fatty acids were found, including sixteen saturated fatty acids (SFA), nine monounsaturated fatty acids (MUFA) and eleven polyunsaturated fatty acids (PUFA). The most abundant SFA in all fish were palmitic acid and stearic acid. However, the most abundant fatty acids in all trout samples were MUFA and PUFA. MUFA were the most abundant fatty acid in farmed brown trout because of high abundance of oleic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmo trutta; Oncorhynchus mykiss; Body composition; Fatty acid composition; Sensory quality.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900752
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Cholesterol changes the fatty acid composition of rat enterocytes BJMBR
Gazzola,J.; Martins,E.F.; Miyasaka,C.K.; Palanch,A.C.; Vecchia,M.G.; Curi,R..
The effect of free cholesterol on the fatty acid composition and growth of rat fetal enterocytes was investigated in the absence and presence of 10% (v/v) fetal calf serum. Cholesterol caused a significant reduction of cell number after 6 and 12 h in culture. The fatty acid composition of enterocytes cultured in the presence of serum was also changed by the presence of 20 µM cholesterol. The fatty acid profile was determined by HPLC using fluorescence detection (325 nm excitation and 395 nm emission). Cholesterol (20 µM) increased the proportion (given in percentage of the total fatty acids) of the following fatty acids in cultured cells: lauric (by 42%), oleic (by 34%), linoleic (by 44%) and gamma-linolenic (by 20%) acids and reduced the proportion of...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Enterocytes; Cholesterol; Fatty acid composition; Cell proliferation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100019
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Determination of sterol and fatty acid compositions, oxidative stability and nutritional value of six walnut (Juglans regia L.) cultivars grown in Portugal IPB - Escola Superior Agrária
Amaral, J.S.; Casal, S.; Pereira, J.A.; Seabra, R.M.; Oliveira, M.B.P.P..
Six cultivars (Franquette, Marbot, Mayette, Mellanaise, Lara, and Parisienne) of walnuts (Juglans regia L.) were collected during the 2001 crop, from Bragança, Portugal. Chemical composition, including moisture, total oil content, crude protein, ash, carbohydrates, and nutritional value, was evaluated. Fat was the predominant component, ranging from 62.3 to 66.5%. Total oil was extracted and analyzed for fatty acids, sterols, oxidative stability, and peroxide value. Fatty acids and sterols were determined by gas-liquid chromatography coupled to a flame ionization detector. Eighteen fatty acids were quantified. Polyunsaturated fatty acids and, in particular, linoleic acid were predominant. â-Sitosterol, ¢5-avenasterol, and campesterol were the major sterols...
Tipo: Article Palavras-chave: Juglans regia L.; Walnut; Chemical composition; Fatty acid composition; Sterol composition.
Ano: 2003 URL: http://hdl.handle.net/10198/628
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Dietary Brazilian red pepper essential oil on pork meat quality and lipid oxidation Ciência Rural
Gois,Franz Dias; Sbardella,Maicon; Lima,Cristiane Bovi de; Migotto,Dannielle Leonardi; Cairo,Pedro Leon Gomes; Garbossa,Cesar Augusto Pospissil; Racanicci,Aline Mondini Calil; Costa,Leandro Batista.
ABSTRACT: The purpose of this study was to evaluate the effects of feeding pigs with diets containing increasing levels of Brazilian red pepper essential oil ( Schinus terebinthifolius Raddi) on the physical attributes, fatty acid profile and oxidative stability of precooked meat. Seventy-two weanling pigs (5.7±0.8kg) were allotted in a completely randomized block design experiment with four treatments, six replicates per treatment, and three animals per experimental unit (pen). Animals were fed with a basal diet supplemented with 0, 500, 1,000, or 1,500mg kg-1 Brazilian red pepper essential oil during the 35-d experimental period. At the end of the experiment, one animal per experimental unit (16.4±2.2kg) was slaughtered to sample Longissimus dorsi muscle...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidative effect; Fatty acid composition; Muscle; Natural feed additive.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000200751
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Dietary neutral lipid level and source in marine fish larvae: Effects on digestive physiology and food intake ArchiMer
Morais, Sofia; Conceicao, L; Ronnestad, I; Koven, W; Cahu, Chantal; Zambonino, Jose-luis; Dinis, M.
A growth depressing effect of high dietary neutral lipid levels in marine fish larvae has been reported. This may be a result of a decrease in the efficiency or activity of digestive enzymes, a reduction in absorption efficiency and/or a decrease in food intake. The present work reviews recent studies carried out on commercially valuable species (Atlantic herring, Senegalese sole, European seabass and gilthead seabream) that investigated the effects of neutral lipid level and lipid source (fatty acid composition) on some of these key factors influencing larval growth. The results seem to collectively indicate that lipid transport from the enterocytes into the body may be more problematic in larval stages dealing with high neutral lipid diets than lipolytic...
Tipo: Text Palavras-chave: Ingestion; Lipid droplets; Digestion; Absorption; Fatty acid composition; Lipid quantity.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3029.pdf
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Differences on the Yolk Lipid, Cholesterol and Fatty Acid Composition of Some Commercial Strains OAK
三好, 俊三; 光本, 孝次.
Palavras-chave: 卵黄脂質; コレステロール; 脂肪酸組成; 市販鶏種; Yolk lipid; Cholesterol; Fatty acid composition; Commercial strain.
Ano: 1994 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/446
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Does dietary n-3 highly unsaturated fatty acids level influence the European sea bass (Dicentrachus labrax) capacity to adapt to a high temperature? ArchiMer
Person, Jeannine; Skalli, Ali; Dulau, Barbara; Le Bayon, Nicolas; Le Delliou, Herve; Robin, Jean.
Triplicate groups of European sea bass juveniles (60 g) were fed for 84 days two experimental diets containing either vegetable or marine oil (0.4 and 2.2% n-3 highly unsaturated fatty acid [n-3 HUFA] dry matter in LD and HD, respectively) and held at 22 or 29 degreesC (LD-22, LD-29, HD-22 and HD-29 groups). Significant differences in mass gain between the two diets and temperatures were, observed from day 28 onwards, and on day 84, weight was 18% higher in the HD-29 group than in the LD-22 group (the highest and lowest values. respectively). Over the 84-day period-SGR was significantly affected both by temperatures and diets (P=6x10(-4) and 2x10(-4) respectively), averaging 1.01 and 1.07 in the HD-22 and HD-29 groups compared to 0.92 and 1.0 in the LD-22...
Tipo: Text Palavras-chave: Oxygen consumption; Fatty acid composition; Growth; Temperature; N 3 HUFA; European sea bass.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-502.pdf
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Fatty acid and sugar compositions, and nutritional value of five wild edible mushrooms from Northeast Portugal IPB - Escola Superior Agrária
Barros, Lillian; Baptista, Paula; Correia, Daniela M.; Casal, S.; Oliveira, M.B.P.P.; Ferreira, Isabel C.F.R..
The chemical composition and nutritional value of five wild edible mushroom species (Agaricus arvensis, Lactarius deliciosus, Leucopaxillus giganteus, Sarcodon imbricatus, Tricholoma portentosum) commonly consumed in the Tra´s-os-Montes region of Northeast Portugal was determined. Chemical composition evaluation included moisture, total oil content, crude protein, ash, carbohydrates, and nutritional value determination. The macronutrient profile in general revealed that the wild mushrooms were rich sources of protein and carbohydrates, and had low amounts of fat. On the basis of the proximate analysis, it can be calculated that an edible portion of 100 g of these mushrooms provides, on average, 28 kcal (118 kJ). The analysis of fatty acid composition,...
Tipo: Article Palavras-chave: Wild edible mushrooms; Chemical composition; Fatty acid composition; Sugar composition; Nutritional value.
Ano: 2007 URL: http://hdl.handle.net/10198/832
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Fatty acid composition, antioxidant and antibacterial properties of the microwave aqueous extract of three varieties of Labisia pumila Benth Biol. Res.
Karimi,Ehsan; Jaafar,Hawa ZE; Ghasemzadeh,Ali; Ebrahimi,Mahdi.
BACKGROUND: The present study was conducted in order to evaluate the fatty acid profile, anti-oxidant and anti-bacterial activities from the microwave aqueous extract of the leaves of three different varieties of Labisia pumila Benth. RESULTS: The chemical analysis of the extract showed that fatty acids (palmitic, palmitoleic, stearic, oleic, linoleic and α-linolenic) acid as the main components in three varieties of L. pumila leaves. Furthermore, the obtained results of the anti-oxidant revealed that L. pumila var. alata contained higher anti-oxidative activities compared to var. pumila and var. lanceolata. However, these values were lower than the tested anti-oxidant standards. On the other hand, the aqueous leaf extracts in all three varieties...
Tipo: Journal article Palavras-chave: Fatty acid composition; Microwave aqueous extraction; Anti-oxidant activities; Anti-bacterial activities.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100008
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Gene transfer of Chlorella vulgaris n-3 fatty acid desaturase optimizes the fatty acid composition of human breast cancer cells BJMBR
Xue,Meilan; Ge,Yinlin; Zhang,Jinyu; Wang,Qing; Hou,Lin.
Chlorella vulgaris has the gene of n-3 fatty acid desaturase (CvFad3), which can synthesize the precursor of n-3 polyunsaturated fatty acids (PUFAs) or convert n-6 to n-3 PUFAs. The objective of the present study was to examine whether the CvFad3 gene from C. vulgaris can be functionally and efficiently expressed in human breast cancer cells and whether its expression can exert a significant effect on cell fatty acid composition. We inserted the CvFad3 gene into the plasmid pEGFP-C3 to construct the eukaryotic expression vector pEGFP-C3-n-3 and to express the n-3 Fad gene in human breast cancer cells (MCF-7 cells). Transfection of MCF-7 cells with the recombinant vector resulted in a high expression of n-3 fatty acid desaturase. Lipid analysis indicated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorella vulgaris; CvFad3 gene; Fatty acid desaturase; Recombinant expression vector; Fatty acid composition; Breast cancer cells.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200006
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Identification of QTL with effects on intramuscular fat content and fatty acid composition in a Duroc × Large White cross Inra
Sanchez, M.P.; Iannuccelli, N.; Basso, B.; Bidanel, J.P.; Billon, Y.; Gandemer, G.; Gilbert, H.; Larzul, C.; Legault, C.; Riquet, J.; Milan, D.; Le Roy, P..
Tipo: Journal Article Palavras-chave: QTL; Intramuscular fat content; Fatty acid composition.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20075242859a&uri=/notices/prodinra1/2009/12/
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Incorporation of dietary trans monounsaturated fatty acids into tissues of Walker 256 tumor-bearing rats BJMBR
Moreira,N.X.; Curi,R.; Padovese,R.; Mancini-Filho,J..
The correlation between dietary trans fatty acids and neoplasia was examined in the present study. Walker 256 tumor-bearing and control rats were fed a trans monounsaturated fatty acid (MUFA)-rich diet for 8 weeks and the incorporation of trans fatty acids by tumor tissue was examined. Also, the effect of tumor growth on trans fatty acid composition of plasma and liver, and the content of thiobarbituric acid-reactive substances (TBARS) was determined. Walker 256 tumor cells presented both trans and cis MUFAs given in the diet. The equivalent diet proportions were 0.66 for trans and 1.14 for cis. Taking into consideration the proportion of trans MUFAs in plasma (11.47%), the tumor incorporated these fatty acids in a more efficient manner (18.27%) than the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trans fatty acids; Walker 256 tumor; Fatty acid composition; Lipid peroxidation.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400010
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Pavlova lutheri: Production, preservation and use as food for Crassostrea gigas larvae ArchiMer
Ponis, Emanuele; Parisi, G; Chini Zittelli, G; Lavista, F; Robert, Rene; Tredici, M. R..
The haptophyte Pavlova lutheri was cultivated in a 4-l flat alveolar photobioreactor (FAP) in semi-continuous mode during 35 days, achieving a mean volumetric productivity of 0.4 g l-1 day-1. The microalgal culture was harvested from the photobioreactor at an average concentration of 3.5 g l-1 and subjected to different storage conditions in which the effects of temperature (1 °C, 4 °C and 25 °C), air supply and preservative (ascorbic or propionic acid) addition on the viability of the stored suspensions were investigated. Low temperatures (1 and 4 °C) and air-bubbling extended the shelf-life of the stored suspension, while no beneficial effects of ascorbic or propionic acid were found. The influence of temperature and air-bubbling on fatty acid content...
Tipo: Text Palavras-chave: Crassostrea gigas; Fatty acid composition; Mollusc nutrition; Photobioreactor; Pavlova lutheri; Preservation; Algal suspensions.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4194.pdf
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Perfil de ácidos gordos no músculo longissimus thoracis et lumborum de suínos da raça Bísara IPB - Escola Superior Agrária
Carvalho, Marieta; Oliveira, M.B.P.P.; Azevedo, Jorge.
A carne de porco é um dos alimentos mais consumidos em todo o mundo, representando em Portugal cerca de 42,5% da carne total consumida. O porco Bísaro tem um teor médio de gordura para os músculos longissimus thoracis et lumborum, semimembranosus e bíceps femoris de 4,89±1,66 g/100g e um perfil de ácidos gordos especialmente rico em monoinsaturados. O perfil dos ácidos gordos é um indicador da qualidade da carne, podendo ser utilizado para avaliar a autenticidade dos seus produtos [1]. Este trabalho tem como objectivo apresentar os resultados obtidos na determinação da gordura total e perfil dos ácidos gordos do músculo longissimus thoracis et lumborum em suínos da raça Bísara. Utilizaram-se 45 porcos (28 fêmeas e 17 machos), pertencentes à Unidade...
Tipo: ConferenceObject Palavras-chave: Fatty acid composition; Bísaro pigs; Pork quality; Intramuscular fats; Longissimus thoracis et lumborum.
Ano: 2011 URL: http://hdl.handle.net/10198/5938
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Physicochemical characteristics and fatty acid composition of tomato seed oils from processing wastes Scientia Agricola
Cantarelli,P.R.; Regitano-d'Arce,M.A.B.; Palma,E.R..
The major component of tomato processing industry wastes is seed. Samples of tomato (Petomech var.) pomace from industries of São Paulo state submitted to Hot and Cold Break treatments, were spontaneously fermented and washed to separate seeds. The oils were analysed for specific gravity, iodine and saponifícation numbers, refractive index, viscosity and fatty acid composition. Except for saponifícation number, Hot and Cold Break seed oils were very similar. In both treatments palmitic acid was the major saturated fatty acid, followed by stearic acid. Linoleic acid was the major unsaturated fatty acid followed by oleic acid. Both oleic and linoleic acids added up to over 60% of total fatty acids, being higher in Cold Break seed oils. Both treatments...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tomato seed oil; Fatty acid composition; Physicochemical characteristics; Industrial wastes.
Ano: 1993 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161993000100016
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Requirement of n-3 long chain polyunsaturated fatty acids for European sea bass (Dicentrarchus labrax) juveniles: growth and fatty acid composition ArchiMer
Skalli, Ali; Robin, Jean.
European sea bass juveniles (14.4+/-0.1 g mean weight) were fed diets containing different levels of fish oil then of n-3 highly unsaturated fatty acids (n-3 HUFA) for 12 weeks. The fish performance as well as fatty acid (FA) composition of neutral and polar lipids from whole body after 7 and 12 weeks feeding were studied. The requirements of juvenile sea bass for n-3 highly unsaturated fatty acids (n-3 HUFA) were studied by feeding fish diets containing six different levels of n-3 HUFA ranging from 0.2% to 1.9% of the diet, with approximately the same DHA/EPA ratio (1.5:1). The growth rate at the end of the trial showed significant differences. Fish fed low dietary n-3 HUFA (0.2% DM of the diet) showed significantly lower growth than the diet 3 (0.7%),...
Tipo: Text Palavras-chave: N 3 HUFA; Growth; Fatty acid composition; European sea bass Dicentrarchus labrax.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-501.pdf
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Some physico-chemical properties, fatty acid compositions, macro-micro minerals and sterol contents of two variety tigernut tubers and oils harvested from East Mediterranean region Ciênc. Tecnol. Aliment.
DUMAN,Erman.
Abstract This research on Yellow sugar and Honey tuber varieties tigernut tubers’ oils physico-chemical properties were investigated to collected from Adana province in Turkey. The 1000 seed weight, width, length, moisture, ash and ascorbic acid values of yellow sugar and honey wax tubers were established as 343,496 and 327,524 g, 7.61 and 8.76 mm, 17.07 and 15.23 mm, 6.41 and 6.45%, 1.19 and 1.38% and 67.34 and 58.81 mg/kg, respectively. Free fatty acidity, peroxide values, refractive index, density, iodine number, total sterol, saponifiable matter, unsaponifiable matter, mineral values of yellow sugar and honey tuber tigernut oils were determined. The palmitic, stearic, oleic and linoleic acid contents of yellow sugar and honey tuber oils were determined...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tigernut; Variety; Minerals; Fatty acid composition; Sterols; Food.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000600610
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Ultrasound-assisted aqueous enzymatic extraction of oil from perilla seeds and determination of its physicochemical properties, fatty acid composition and antioxidant activity Ciênc. Tecnol. Aliment.
LI,Huizhen; ZHANG,Zhijun; HE,Dongliang; XIA,Yaoyao; LIU,Qingye; LI,Xiaojun.
Abstract Response surface methodology (RSM) was used to optimize ultrasound-assisted aqueous enzymatic extraction (UAAEE) conditions for perilla seed oil. Under the optimum conditions—which were a liquid-to-solid ratio of 4.4:1, a hydrolysis time of 2.66 h, a hydrolysis temperature of 50.87 °C and an ultrasound treatment time of 24.74 min—an oil yield of 31.34% was obtained. Comparisons of the physicochemical characteristics of oil obtained using UAAEE with those of oil obtained by solvent extraction (SE oil) and cold pressing extraction (CPE oil) revealed similar refractive indices and saponification values, but UAAEE oil had a higher iodine value and better stability against oxidation, with a low peroxide value. UAAEE oil contained higher levels of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Perilla seed oil; Ultrasound-assisted aqueous enzymatic extraction; Physical and chemical characteristics; Fatty acid composition; Antioxidant activities.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000500071
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องค์ประกอบกรดไขมันและสารแกมมาโอรีซานอลของไขมันน้ำมันรำข้าวและไขมันชนิดอื่นและคุณภาพของบัตเตอร์ครีมที่ผลิตได้ Thai Agricultural
Tida Sirisukpornchai; Pravit Santivatana.
The objectives of this study were to analyze and compare fatty acid composition, slip melting point and gamma-oryzanol content among rice bran oi l shortening, palm shortening, and butter. Three formulas of butter cream were prepared, by varying type of fat at 25%. Then the sensory evaluation were assessed and the percentage difference in volume among the three formulas were measured by measuring the specific volume of butter cream made from rice bran oil shortening, palm shortening and butter blend. Saturated fatty acid, monounsaturated fatty acid, and polyunsaturated fatty acid contents were 35.43±0.03%, and 36.73±0.10%, 27.84±0.13% in rice bran oil shortening, 66.36±0.12%, 28.28±0.04%, 5.36±0.04% in palm shorte ning and 61.49±0.04%, 32.98±0.06%,...
Tipo: PhysicalObject Palavras-chave: Rice bran oil shortening; Fatty acid composition; Butter cream; Gramma-oryzanol; Quality; ไขมันน้ำมันรำข้าว; กรดไขมัน; บัตเตอร์ครีม; แกมมาโอรีซานอล.
Ano: 2012 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5397
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