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| Registros recuperados: 36 | |
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| VANEGAS K. G.; RENDSVIG, J. K. H.; JARCZYSKA, Z. D.; CORTES, M. V. de C. B.; VAN ESCH, A. P.; MORERA-GÓMEZ, M.; CONTESINI, F. J.; MORTENSEN, U. H.. |
| The introduction of CRISPR technologies has revolutionized strain engineering in filamentous fungi. However, its use in commercial applications has been hampered by concerns over intellectual property (IP) ownership, and there is a need for implementing Cas nucleases that are not limited by complex IP constraints. One promising candidate in this context is the Mad7 enzyme, and we here present a versatile Mad7-CRISPR vector-set that can be efficiently used for the genetic engineering of four different Aspergillus species: Aspergillus nidulans, A. niger, A. oryzae and A. campestris, the latter being a species that has never previously been genetically engineered. We successfully used Mad7 to introduce unspecific as well as specific template-directed... |
| Tipo: Artigo de periódico |
Palavras-chave: Filamentous fungi; CRISPR; Mad7; Fungal strain engineering; Fungo; Aspergillus; Genetic engineering; Fungi. |
| Ano: 2023 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1151460 |
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| Baracho,Marta S.; Baracho,Ivanhoé R.. |
| Mutations related to gene methG1 of Aspergillus nidulans were analyzed, in order to study a mathematical model for the determination of the mutation rate per nucleus per generation, in filamentous fungi. A replica plating technique was used to inoculate, in a single operation, 26 colonies of the strain, into Petri dishes containing culture medium, and the nine central colonies were analyzed for size and number of conidia in each colony. Using this technique, several central colonies were analyzed with regard to the appearance of mutation, and the number and type of reversions were determined for each colony. The frequencies obtained for each reversion were analyzed, in order to verify if their distribution was in accordance with that of Greenwood and Yule.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus nidulans; Mutation rate; Spontaneous mutation; Fungi; Filamentous fungi. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100014 |
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| Soares,Luciana Arantes; Sardi,Janaína de Cássia Orlandi; Gullo,Fernanda Patrícia; Pitangui,Nayla de Souza; Scorzoni,Liliana; Leite,Fernanda Sangalli; Giannini,Maria José Soares Mendes; Almeida,Ana Marisa Fusco. |
| Millions of people and animals suffer from superficial infections caused by a group of highly specialized filamentous fungi, the dermatophytes, which only infect keratinized structures. With the appearance of AIDS, the incidence of dermatophytosis has increased. Current drug therapy used for these infections is often toxic, long-term, and expensive and has limited effectiveness; therefore, the discovery of new anti dermatophytic compounds is a necessity. Natural products have been the most productive source for new drug development. This paper provides a brief review of the current literature regarding the presence of dermatophytes in immunocompromised patients, drug resistance to conventional treatments and new anti dermatophytic treatments. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dermatophytes; Filamentous fungi; Natural products; Synthetic product. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400003 |
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| SOBRAL,LAUREANA V.; MELO,KELLY N.; SOUZA,CLECIANA M.; SILVA,SÍLVIO F.; SILVA,GILVANIA L.R.; SILVA,ANDRESSA L.F.; WANDERLEY,KATHARINE A.A.; OLIVEIRA,IDJANE S.; CRUZ,ROBERTA. |
| ABSTRACT To the fungal microbiota the UFPE and biotechnological potential enzymatic and antimicrobial production. Air conditioned environments were sampled using a passive sedimentation technique, the air I ratio and the presence of aflatoxigenic strains evaluated for ANVISA. Icelles were to determine the enzymatic activity of lipase, amylase and protease metabolic liquids to determine antimicrobial activity. Diversity was observed in all CAV environments, CFU/m3 ranged from 14 to 290 and I/E ratio from 0.1 to 1.5. The of the fungal genera were: Aspergillus (50%), Penicillium (21%), Talaromyces (14%), Curvularia and Paecilomyces (7% each). Aspergillus sydowii (Bainier & Sartory) Thom & Church presented enzymatic activity and the Talaromyces... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Air quality; Bacteria; Enzymes; Filamentous fungi; Refrigerated environments. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502327 |
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| Almeida,Paula Zaghetto de; Pereira,Marita Gimenez; Carvalho,Caio Cesar de; Heinen,Paulo Ricardo; Ziotti,Luciana Sobrani; Messias,Josana Maria; Jorge,João Atilio; Polizeli,Maria de Lourdes Teixeira de Moraes. |
| Abstract Filamentous fungi are widely diverse and ubiquitous organisms. Such biodiversity is barely known, making room for a great potential still to be discovered, especially in tropical environments - which are favorable to growth and species variety. Filamentous fungi are extensively applied to the production of industrial enzymes, such as the amylases. This class of enzymes acts in the hydrolysis of starch to glucose or maltooligosaccharides. In this work twenty-five filamentous fungi were isolated from samples of decomposing material collected in the Brazilian Atlantic Forest. The two best amylase producers were identified as Aspergillus brasiliensis and Rhizopus oryzae. Both are mesophilic, they grow well in organic nitrogen-rich media produce great... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amylase; Filamentous fungi; Aspergillus brasiliensis; Rhizopus oryzae; Glucoamylase; Bioprospection. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032017000300204 |
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| Tagliari,Cristiane Vanessa; Sanson,Raquel K.; Zanette,André; Franco,Telma Teixeira; Soccol,Carlos Ricardo. |
| Various microorganisms including bacteria, yeast and fungi can degrade caffeine. There are few publications about caffeine degradation pathway in filamentous fungi, mainly by solid-state fermentation (SSF). Studies were carried out on degradation of caffeine and their metabolites by filamentous fungi in SSF using coffee husk as substrate. The purpose of this work was to investigate the caffeine degradation pathway by Rhizopus delemar in packed bed column fermenter and to compare this degradation metabolism with glass flasks fermentation. The methylxanthines were quantified by HPLC analysis. The experiments were realized with the optimized conditions in previous experiments: pH 6.5, 28ºC, inoculation rate 10(6) spores/g substrate, aeration rate 60 mL/min... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Decaffeination; Fermentation; Caffeine; Theophylline; Bioreactor; Filamentous fungi. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500035 |
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| Souza,Leiliane Cristine de; Zambom,Maximiliane Alavarse; Pozza,Magali Soares dos Santos; Neres,Marcela Abbado; Radis,Ana Cláudia; Borsatti,Liliane; Castagnara,Deise Dalazen; Gundt,Simoni. |
| This research study was conducted to evaluate, by means of microbiological analyses, the preservation of wet brewery waste stored under aerobic and anaerobic conditions, regarding the development of filamentous fungi, yeasts and lactic-acid bacteria. The following treatments were used: untreated brewery waste, pre-dried brewery waste silage, brewery waste silage with effluent drainage and BW silage without effluent drainage. Silos made of PVC and equipped with Bunsen valves to allow gases to escape were used. Dry matter (DM), crude protein (CP), temperature (°C) and pH in the untreated BW, in the stored brewery waste and in the brewery waste silage upon silo opening, after 60 days of ensilage were analyzed. A completely randomized design was used. The data... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Filamentous fungi; Lactic acid bacteria; Silage; Yeasts. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000100027 |
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| PALOMINO García,Lady Rossana; BIASETTO,Carolina Rabal; ARAUJO,Angela Regina; BIANCHI,Vanildo Luiz Del. |
| Abstract The use of agroindustrial residues is an economical solution to industrial biotechnology. Coffee husk and pulp are abounding residues from coffee industry which can be used as substrates in solid state fermentation process, thus allowing a liberation and increase in the phenolic compound content with high added value. By employing statistical design, initial moisture content, pH value in the medium, and the incubation temperature were evaluated, in order to increase the polyphenol content in a process of solid state fermentation by Penicillium purpurogenum. The main phenolic compounds identified through HPLC in fermented coffee residue were chlorogenic acid, caffeic acid, and rutin. Data obtained through HPLC with the radical absorbance capacity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffee husk; Coffee pulp; Filamentous fungi; Bioprocess design; Antioxidant activity; Chlorogenic acid. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000400704 |
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| Guimarães, Cíntia Gonçalves; Bonfá, Caroline Salezzi; Evangelista, Antônio Ricardo; Santos, Alexandre Soares dos; Pantoja, Lílian de Araújo; Castro, Gustavo Henrique de Frias. |
| The elephant grass presents problems during the fermentation of the ensiled material, being necessary use of additives. This study aimed to evaluate the population of yeast and filamentous fungi, enterobacterias, contents of acetic and butyric acids and ethanol production, in elephant grass silages added with different levels of macaúba cake at opening times. This was a 3 x 6 factorial completely randomized experimental design, with three inclusion levels of the macaúba cake (0, 10 and 20%) and six opening times (1, 5, 10, 20, 40 and 60 days after ensiling), with four replications. For all the studied variables, there was a difference in the interaction among levels and times, and an increasing linear behavior only for the contents of acetic acid, for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterobacterias; Filamentous fungi; Opening times; Organic acids; Yeast fungi.. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/42523 |
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| Silva Júnior,Flavio Manoel Rodrigues da; Pereira,Sônia Valéria. |
| The purpose of this study was to assess the impact of metallurgical industry wastes on the semiarid soil microbiota using physico-chemical and microbiological parameters, highlighting the filamentous fungi assembly. Soil samples were collected in an area of industrial waste deposit contaminated with lead and mixed with natural soil (control soil) in seven different concentrations (0, 7.5, 15, 30, 45, 60 and 100%). The results showed alterations on the physico-chemical properties of the soil treated with industrial wastes, with a gradate increase of the soil's pH (5.6-10.4) and electrical conductivity (0.3-14.7 dS m-1) and also reduction of organic matter (7.0-1.8%). The use of microbiological parameters (fungal richness and diversity, CO2emission, and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chaetomium; Microbial activity; Contaminated soils; Filamentous fungi; Lead. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500723 |
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| AMAZONAS, D. R.; SOUTO, A. L.; VIEIRA, F. S.; CARNEIRO, A. A. J.; VIANNA, R. B.; MENDES, T. D.; LIMA, K. S.; RIBEIRO, J. A. A.; DAMASO, M. C. T.; RODRIGUES, C. M.; ABDELNUR, P. V.. |
| Tipo: Separatas |
Palavras-chave: DIMS; Filamentous fungi; Metabolomics; Mass spectrometry; Biotechnology. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113564 |
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| Santos-Ebinuma,Valéria Carvalho; Roberto,Inês Conceição; Teixeira,Maria Francisca Simas; Pessoa Jr.,Adalberto. |
| Safety issues related to the employment of synthetic colorants in different industrial segments have increased the interest in the production of colorants from natural sources, such as microorganisms. Improved cultivation technologies have allowed the use of microorganisms as an alternative source of natural colorants. The objective of this work was to evaluate the influence of some factors on natural colorants production by a recently isolated from Amazon Forest, Penicillium purpurogenum DPUA 1275 employing statistical tools. To this purpose the following variables: orbital stirring speed, pH, temperature, sucrose and yeast extract concentrations and incubation time were studied through two fractional factorial, one full factorial and a central composite... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Natural colorants; Filamentous fungi; Optimization; Submerged culture. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200049 |
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| Ramos, Darlê Martins Barros; Faculdades Unidas do Norte de Minas- FUNORTE- Montes Claros- MG; Silva, Cristina Ferreira; Departamento de Biologia- Universidade Federal de Lavras- CP 3037 , Lavras- MG; Batista, Luís Roberto; Departamento de Ciência dos Alimentos- Universidade Federal de Lavras- CP 3037 Lavras- MG; Schwan, Rosane Freitas; Departamento de Biologia- Universidade Federal de Lavras- CP 3037 , Lavras- MG. |
| O café é um produto nacional com grande expressão para a economia brasileira. O uso excessivo de fungicidas tem levado a pesquisas sobre formas alternativas como o controle biológico. Objetivou-se avaliar o potencial antagônico de leveduras em co-cultivo com fungos filamentosos. Isolados das espécies Debaryomyces hansenii (UFLACF 889 e UFLACF 847) e Pichia anomala (UFLACF 710 e UFLACF 951) foram inoculados (103 a 106 células mL-1) com três espécies de fungos filamentosos, Aspergillus ochraceus, A. parasiticus e Penicillium roqueforti (103 a 106 esporos mL-1). A avaliação do crescimento micelial e a contagem de esporos foram realizadas durante 21 dias. Observou-se que o isolado UFLACF 889 apresentou, em média, maior efeito inibitório na produção de... |
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Palavras-chave: 2.12.00.00-9 Leveduras; Fungos filamentosos; Biocontrole; Café; Debaryomyces; Pichia 2.12.00.00-9 Yeasts; Filamentous fungi; Biocontrol; Coffee; Debaryomyces; Pichia. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3361 |
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| Registros recuperados: 36 | |
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