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| Villas-Bôas,C.S.A.; Conceição,T.M.; Ramírez,J.; Santoro,A.B.M.; Da Poian,A.T.; Montero-Lomelí,M.. |
| Dengue virus (DV)-induced changes in the host cell protein synthesis machinery are not well understood. We investigated the transcriptional changes related to initiation of protein synthesis. The human hepatoma cell line, HepG2, was infected with DV serotype 2 for 1 h at a multiplicity of infection of one. RNA was extracted after 6, 24 and 48 h. Microarray results showed that 36.5% of the translation factors related to initiation of protein synthesis had significant differential expression (Z-score ≥ ±2.0). Confirmation was obtained by quantitative real-time reverse transcription-PCR. Of the genes involved in the activation of mRNA for cap-dependent translation (eIF4 factors), eIF4A, eIF4G1 and eIF4B were up-regulated while the negative regulator of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dengue virus; Flavivirus; Protein synthesis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100004 |
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| BUENO ANGARITA,Claudia Liliana; MORALES,Liliana; VELANDIA-ROMERO,Myriam Lucia; CALDERÓN-PELÁEZ,María Angélica; CHAPARRO-OLAYA,Jacqueline. |
| RESUMEN Algunos virus envueltos usurpan la maquinaria celular ESCRT (complejo de clasificación endosomal requerido para el transporte) para llevar a cabo funciones como la transcripción, la traducción, el ensamblaje y la liberación de partículas virales desde las células huésped. Aunque esta estrategia ha sido estudiada principalmente en retrovirus, son varios los virus envueltos que la usan. El objetivo del trabajo fue explorar la participación de una proteína accesoria de ESCRT, la proteína Alix, en la transcripción, traducción, ensamblaje y liberación del virus dengue (DENV), así como su interacción con la proteína viral NS3. Células A549 infectadas con DENV2 fueron tratadas con pequeños ARN de interferencia (siRNA) para disminuir la expresión... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Flavivirus; Maquinaria ESCRT; Procesos fisiológicos virales; Serina endopeptidasa. |
| Ano: 2019 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000300509 |
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| CASTAÑEDA,NADIA Y; CASTELLANOS,JAIME E; ZAPATA,ANGELA C; BELLO,FELIO. |
| Los cultivos celulares de mosquitos son frecuentemente utilizados para el aislamiento, identificación y caracterización de arbovirus. Para el estudio de los virus dengue (VDEN) y virus de fiebre amarilla (VFA) se emplean, principalmente, la línea celular C6/36 de Aedes albopictus y la línea celular AP61, obtenida de Aedes pseudoscutellaris. La línea celular de A. aegypti AEGY28, previamente obtenida a partir de tejidos embrionarios del vector, se utilizó en el presente trabajo para evaluar la susceptibilidad a la infección por VDEN y VFA. Para ello, los cultivos celulares se ensayaron a diferente multiplicidad de infección con los aislados clínicos de virus dengue tipo 2 (COL789, MOI: 1 y 5) y VFA (V341, MOI 0,02). Posteriormente se realizó la detección de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Flavivirus; Línea celular; Virus dengue; Fiebre amarilla; Arbovirus; Infección. |
| Ano: 2007 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2007000200004 |
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| de Moraes,F.M.; Espósito,D.L.A.; Klein,T.M.; da Fonseca,B.A.L.. |
| Clinical manifestations of Zika, dengue, and chikungunya virus infections are very similar, making it difficult to reach a diagnosis based only on clinical grounds. In addition, there is an intense cross-reactivity between antibodies directed to Zika virus and other flaviviruses, and an accurate Zika diagnosis is best achieved by real-time RT-PCR. However, some real-time RT-PCR show better performance than others. To reach the best possible Zika diagnosis, the analytic sensitivity of some probe-based real-time RT-PCR amplifying Zika virus RNA was evaluated in spiked and clinical samples. We evaluated primers and probes to detect Zika virus, which had been published before, and tested sensitivity using serum spiked and patient samples by real-time RT-PCR.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zika virus; Flavivirus; Real time RT-PCR; Diagnosis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600618 |
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| Casseb,Alexandre R; Cruz,Andrea V; Jesus,Iroleide S; Chiang,Jannifer O; Martins,Lívia C; Silva,Sandro P; Henriques,Daniele F; Casseb,Livia MN; Vasconcelos,Pedro Fernando C. |
| BackgroundThe state of Pará encompasses 26% of Brazilian Amazon where an enormous diversity of arboviruses has been found. This study aimed to assess the prevalence and distribution of hemagglutination-inhibition antibodies against antigens of six Flavivirus(yellow fever virus, Ilheus virus, Saint Louis encephalitis virus, Cacipacore virus, Bussuquara virus and Rocio virus) in water buffaloes in Pará state, Brazil. The prevalence of antibodies in these farm animals is important to determine the circulating arboviruses.FindingsAll investigated arboviruses were detected in the species studied and our results indicate that water buffaloes are susceptible to Flavivirusinfection. Furthermore, there is solid evidence of active circulation of these viruses in the... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Flavivirus; Water buffaloes; Hemagglutination inhibition test. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200600 |
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| The yellow fever (YF) virus is the prototype flavivirus. The use of molecular techniques has unraveled the basic mechanisms of viral genome structure and expression. Recent trends in flavivirus research include the use of infectious clone technology with which it is possible to recover virus from cloned cDNA. Using this technique, mutations can be introduced at any point of the viral genome and their resulting effect on virus phenotype can be assessed. This approach has opened new possibilities to study several biological viral features with special emphasis on the issue of virulence/attenuation of the YF virus. The feasibility of using YF virus 17D vaccine strain, for which infectious cDNA is available, as a vector for the expression of heterologous... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Flavivirus; Yellow fever virus; Attenuation; Vaccine; Gene expression. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200002 |
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