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| Registros recuperados: 22 | |
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| Dias,L.M.K.; Sales,J.N.S.; Viau,P.; Barros,M.B.P.; Nicolau,S.S.; Simões,L.M.S.; Alves,N.G.; Alonso,M.A.; Valentim,R.; Oliveira,C.A.. |
| ABSTRACT The aim of this study was to evaluate hCG treatment on ovarian response and on pregnancy rate using a 9-day oestrus synchronization protocol in Santa Ines ewes. On a random oestrus cycle day, ewes received an intravaginal progesterone device (Primer-PR®, Tecnopec, Brazil). Nine days later (Day 9), 30µg of d-cloprostenol (Prolise®, Syntex, Argentina) and 250IU of eCG (Folligon®, Intervet, Brazil) were administered and the progesterone device was removed. This moment, the ewes were randomly assigned on two groups: Control Group and hCG Group. In the hCG Group, the ewes received 500IU of hCG (Vetecor®, Hertape-Calier, Spain) 24h after device removal. In the Control Group, the ewes did not receive any ovulation inductor. Control and hCG Groups ewes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Progesterone; Follicle; Ovulation; Synchronization. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100122 |
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| MAGATA, Fumie; ISHIDA, Yoshikazu; MIYAMOTO, Akio; FURUOKA, Hidefumi; INOKUMA, Hisashi; SHIMIZU, Takashi; 宮本, 明夫; 古岡, 秀文; 猪熊, 壽; 清水, 隆. |
| We investigated the concentration of the bacterial endotoxin lipopolysaccharide (LPS) in the blood, ovarian follicular fluid and uterine fluid of a clinical case of bovine metritis. A 2-year-old lactating Holstein cow exhibited continuous fever >39.5°C for more than 2 weeks after normal calving. The cow produced a fetid, watery, red-brown uterine discharge from the vagina and was diagnosed with metritis. The LPS concentrations in plasma and uterine fluid were 0.94 and 6.34 endotoxin units (EU)/ml, respectively. One of seven follicles showed an extremely high level of LPS (12.40 EU/ml) compared to the other follicles (0.62–0.97 EU/ml). These results might suggest the presence of high concentration of LPS in follicles in cows with postpartum metritis. |
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Palavras-chave: Dairy cow; Follicle; Lipopolysaccharide; Metritis. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4043 |
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| Carvalho,Nelcio Antonio Tonizza de; Carvalho,Júlia Gleyci Soares de; Sales,José Nélio de Sousa; Macari,Rodrigo Caron; Baruselli,Pietro Sampaio. |
| ABSTRACT: The aim of this study was to evaluate different times for timed artificial insemination (TAI) in buffalo submitted to a P4/E2/eCG-based protocol. In this study, 204 buffaloes were distributed into one of two groups (TAI56, n=103 and TAI64, n=101). At a random stage of the oestrous cycle (Day 0 = D0), in the morning (TAI56, a.m.) or afternoon (TAI64, p.m.), buffaloes received an intravaginal progesterone device (P4; 1.0 g) plus EB (2.0 mg i.m.). On D9 a.m. (TAI56) or p.m. (TAI64), the P4 was removed and buffaloes received PGF2a (0.53 mg i.m. sodium cloprostenol) and eCG (400 IU i.m.). On D10 a.m. (TAI56) or p.m. (TAI64), 24 h after P4 removal, buffaloes were treated with EB (1.0 mg i.m.). Buffaloes from TAI56 and TAI64 were inseminated 56 and 64 h... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anoestrous; Artificial insemination; Oestradiol benzoate; Follicle; Induction of ovulation. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000400701 |
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| Domínguez Islas, Manuel. |
| Se evaluó la incorporación del “efecto macho” a un protocolo de superovulación. Se utilizaron 20 ovejas de lana de la raza Suffolk, Hampshire y Dorset, de condición corporal (cc) 3.1 ± 0.4, con edad promedio de 3.9 ± 0.8 años, durante época de transición de anestro a temporada reproductiva en los meses de junio-julio del 2011. Se asignaron 10 ovejas de manera aleatoria en dos lotes. Los tratamientos fueron los siguientes: Tratamiento 1 (T1): se introdujo un CIDR (considerado día 0) a cada ovejas durante 9 días, 48 horas previas al retiro, se administro 500 μg de Cloprostenol, además de 300 UI de Gonadotropina coriónica equina (eCG) y 250 UI de gonadotropinas hipofisarias de cerdo cada 12 horas (48, 36, 24, 12, 0 previas al retiro y 12 h después del... |
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Palavras-chave: Efecto macho; Ovejas; Anestro; Gonadotropinas; Folículo; Ganadería; Maestría; Male effect; Ewes; Anestrus; Gonadotropins; Follicle. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/1779 |
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| Yin,L; Ran,J; Lian,T; Yang,C; Li,S; Liu,Y. |
| ABSTRACT The objective of this study was to analyse the impact of dietary vitamin E supplementation on laying performance, serum reproductive hormones concentration and gene expression in ovary and follicles of anti-season breeding goose. A total of 210 anti-season breeding geese were divided into seven treatments with six replications. Each group was supplied with diets containing different vitamin E (DL-α-tocopherol acetate) contents (0, 10, 20, 40, 80, 160, 320mg/kg). We observed that the egg production and laying rate improved significantly at doses of 10 and 80 mg/kg, while the highest egg weight appeared in the 320 mg/kg group. Meantime, 80 mg/kg of vitamin E supplementation significantly improved the concentration of follicle-stimulating hormone... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vitamin E; Reproductive hormones; Ovary; Follicle; Dicer. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000400310 |
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| Amorim,Lincoln da Silva; Torres,Ciro Alexandre Alves; Siqueira,Luiz Gustavo Bruno; Fonseca,Jeferson Ferreira da; Guimarães,José Domingos; Carvalho,Giovanni Ribeiro de; Alves,Nadja Gomes; Oliveira,Margarida Maria Nascimento Figueiredo de. |
| The effects of feeding urea on embryo production, quality and developmental stages and follicular status at the beginning of a superovulatory treatment of Toggenburg does fed urea diet were investigated in this study. Eighteen females were randomly allocated to receive diets with: no urea (control, n=8), and 2.4% urea dietary dry matter (n=10) UDM. The embryo recovery was performed at day 7 or 8 of estrus cycle by transcervical technique and classified according to quality and developmental stage. The follicular status was determined by transrectal ultrasonography at the first day of FSH injection. The number and quality of embryos were not affected by dietary urea concentration. Embryos from does treated with 2.4% UDM were recovered at advanced stage.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo; Follicle; Goat; Superovulation; Urea. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000200007 |
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| Smok S,Carolina; Rojas R,Mariana. |
| Ovogenesis and foliculogenesis in the mare differ from other farm animals and therefore, gamete manipulation, in vitro fertilization and embryo transfer have been very difficult. The histologic traits of ovaries from 12 mares, in estrus and diestrus were studied. Number and size of follicules and corpora lutea were recorded. The ovarian stroma was evaluated using the pricosirius technique for collagen. A simple morphometric analysis was done using computerized scanner programs. During late estrus, one dominant follicle (46 ± 4mm) is seen. The surrounding stroma contains collagen I and III. By the end of the estrus, one hemorrhagic follicle is seen, plus one or two small follicle (2 mm in size). In early diestrus there is a corpus luteum (43-60 mm) and some... |
| Tipo: Journal article |
Palavras-chave: Follicle; Stroma; Mare ovary; Seasonality. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300007 |
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| TETSUKA, Masafumi; NISHIMOTO, Hiromi; MIYAMOTO, Akio; OKUDA, Kiyoshi; HAMANO, Seizo; 手塚, 雅文; 宮本, 明夫. |
| Glucocorticoids modulate ovarian function in cattle. However, their regulatory mechanisms have not been fully elucidated. In the present study, we examined gene expression of two glucocorticoid-metabolizing enzymes, a bidirectional 11β-HSD type 1 (11HSD1) and a dehydrogenase 11β-HSD type 2 (11HSD2), and glucocorticoid receptor (GR) in bovine follicles during follicular maturation and atresia. Granulosa cells (GCs) and theca interna layers (TIs) were harvested from follicles classified as small growing, dominant, preovulatory, early atretic and late atretic follicles. The expression levels of 11HSD1, 11HSD2 and GR mRNA were quantified by real-time PCR. In the healthy follicles, expression of 11HSD1 mRNA increased as follicles matured, both in GCs and TIs. A... |
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Palavras-chave: Bovine; Cortisol; 11β-HSD; Follicle; Glucocorticoid receptor; MRNA. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2810 |
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| Ozawa, Takahiro; Acosta, Tomas J.; Nakamura, Tadashi; Miyamoto, Akio; 中村, 正; 宮本, 明夫. |
| The presence of gonadotropin-releasing hormone (GnRH)-like protein has been previously demonstrated in the bovine follicle. This GnRH-like protein was purified and concluded to be histone H2A. However, neither GnRH peptide nor specific GnRH-immunoreactivity (GnRH-IR) has been demonstrated in the bovine follicle so far. Thus, this study focused on the detection of specific GnRH-IR using the second-antibody enzyme immunoassay in isolated bovine mature follicles, and on an examination of the direct effect of luteinizing hormone (LH), endothelin-1 (ET-1), and cytokines on the GnRH-IR using an in vitro microdialysis system (MDS). We further examined a cross-reactivity of the GnRH antibody with bovine histone H2A. GnRH-IR was detected in microdialyzed perfusate... |
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Palavras-chave: GnRH; Immunoreactivity; Microdialysis; Follicle; Cow. |
| Ano: 2000 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2970 |
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| ACOSTA, Tomas J.; TETSUKA, Masa; MATSUI, Motozumi; SHIMIZU, Takashi; BERISHA, Bajram; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 清水, 隆; 宮本, 明夫. |
| The aim of the present in vivo study was to monitor real-time fluctuations of cortisol (Cr) in the wall of preovulatory follicles using a microdialysis system (MDS) implanted in the theca layer as well as changes in ovarian venous plasma (OVP) and jugular venous plasma (JVP). Seven cows were superovulated using FSH and prostaglandin F2α injections. Dialysis capillary membranes were surgically implanted into the theca layer of mature follicles and connected to a microdialysis system. Fractions of the perfusates were collected from Day -1 (Day 0=LH surge) to Day 3. No difference in the concentrations of Cr between JVP and OVP was detected throughout the experiment. Circulating concentrations of Cr ranged from 20 to 35 ng/ml 8 h after surgery in ovulatory and... |
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Palavras-chave: Bovine; Cortisol; Follicle; Ovary; Ovulation. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2980 |
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| Hariya, Madoka; Suzuki, Hiroshi. |
| Although vitrification and follicular survival subsequent to xenotransplantation of canine ovarian tissues have been reported, the degree of cryoinjury after the vitrification has not yet been fully examined. Since cryoinjury after vitrification of ovaries has been reported in bovine and humans, this study evaluated canine ovarian tissues after vitrification by immunohistochemistry and TUNEL assay. Ovaries were collected from immature bitches. The ovarian tissue cubes were pretreated with 1 M DMSO. Subsequently, DAP 213 solution (2 M DMSO, 1 M acetamide, 3 M propylene glycol) was added. The cryotubes containing the ovarian tissues were placed on ice for 5 min, then plunged directly into liquid nitrogen. After warming with 0.25 M sucrose, cryopreserved... |
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Palavras-chave: Cryopreservation; Dog; Follicle; Ovary. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4535 |
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| Sheikh Ahmadi,A; Zaghari,M; Shivazad,M; Hassanpour,H; Towhidi,A. |
| To clarify the effects of high (20 and 40% more than normal) and low (20% less than normal) daily feed allowance on egg and body parameters, ovarian morphology and plasma glucose, cholesterol, triacylglycerol, leptin-like hormone, nitrite/nitrate and gene expression of inducible nitric oxide synthase (iNOS) in the granulosa layer of F1 follicle, broiler breeder hens (30-week-old) were fed for 30 days. Egg and body parameters significantly changed between treatments (p<0.05). Effect of different level of feed intake on ovarian morphology parameters was significant (p<0.05), except for white follicles. After 30 days of experiment, plasma nitrite/nitrate (as a index of plasma nitric oxide) and leptin-like hormone increased in FI+20% and FI+40% groups as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler breeder; Follicle; Leptin-like hormone; Nitric oxide; Over/under-fed. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000400004 |
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| Britt,Jack H.. |
| Oocytes in cattle are formed during embryogenesis and develop within individual follicles in the cortex of the ovary. Dormant primordial follicles become active and undergo progressive development at regular intervals commencing during the late fetal stage and continuing throughout adulthood. Once activated, follicles and oocytes in a cohort either grow to maturation and ovulation or undergo atresia, ultimately depleting the ovaries of germ cells. It takes an estimated 100 days for a follicle and its oocyte to reach the mature ovulatory stage. Within an individual, number of follicles in one ovary is similar to number in the other ovary; however, there are large differences among individuals in total number of follicles present in both ovaries. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Follicle; Ovary; Oocyte; Embryo; Fecundity. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982008001300013 |
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| Registros recuperados: 22 | |
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