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| GUIMARAES, D. P.. |
| Geographical information systems are becoming a common information management and analysis tool used in a wide and growing range of application fields. The popularization of GIS techniques request the easy-to-use softwares. From among 12 freeware softwares evaluated, Hypercube (a freeware program produced by the US Army Corps of Engineers Topographic Engineering Center) was considered the most efficient to perform functions related to filter, warp, mosaic, reformat, calibrate, and combine multi and hyper-spectral imagery. FGIS, according with the same criteria, was considered the most efficient to perform shapefile editing programs, digitizer and GIS data query tool. |
| Tipo: Parte de livro |
Palavras-chave: Image processing; Free software; Processamento de imagens; Softwares gratuitos; CBERS; FGIS; Hypercube.; Sensoriamento Remoto.; Remote sensing.. |
| Ano: 2007 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/488002 |
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| Carli,Gabriel José de; Rotela,Abdon Troche; Lubini,Greice; Contiliani,Danyel Fernandes; Candia,Nidia Benítez; Depintor,Thiago S.; Abreu,Fabiano Carlos Pinto de; Simões,Zilá Luz Paulino; Ríos,Danilo Fernández; Pereira,Tiago Campos. |
| Abstract RNA interference (RNAi) is a powerful gene silencing technology, widely used in analyses of reverse genetics, development of therapeutic strategies and generation of biotechnological products. Here we present a free software tool for the rational design of RNAi effectors, named siRNA and shRNA designer (SSD). SSD incorporates our previously developed software Strand Analysis to construct template DNAs amenable for the large scale production of mono-, bi- and trivalent multimeric shRNAs, via in vitro rolling circle transcription. We tested SSD by creating a trivalent multimeric shRNA against the vitellogenin gene of Apis mellifera. RT-qPCR analysis revealed that our molecule promoted a decrease in more than 50% of the target mRNA, in a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene silencing; SiRNA; Multimeric; ShRNA; Free software. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100802 |
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