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Comparison of different methodologies for DNA extraction from Aegla longirostri BABT
Bitencourt,João Vitor Trindade; Roratto,Paula Angélica; Bartholomei-Santos,Marlise Ladvocat; Santos,Sandro.
The aim of this study was to compare some DNA extraction methodologies for Aegla longirostri. The protocols were based on the traditional phenol-chloroform DNA extraction methodology and using a commercial kit for DNA extraction. They differed in tissues used, the addition - or not - of beta-mercaptoethanol to the lysis buffer, times and methods for the animal's conservation (frozen, in ethanol or fresh). Individuals stored at -20°C for a long time supplied lower molecular weight DNA than those stored for a short time. The best yield for the specimens preserved in ethanol was obtained for 15 days storage in 95% ethanol. The kit resulted in a low quantity of high molecular weight DNA. The best protocol for DNA extraction from Aeglidae, and probably for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anomura; Aeglidae; Freshwater crab; DNA extraction.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700010
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Parathelphusa reticulata spec. nov., a new species of freshwater crab from blackwater swamps in Singapore (Crustacea: Decapoda: Brachyura: Gecarcinucoidea) Naturalis
Ng, P.K.L..
A new species of gecarcinucoid crab, Parathelphusa reticulata spec. nov. is described from blackwater swamps in Singapore. Parathelphusa reticulata is characterized by its relatively small adult size, diagnostic colour pattern, swollen carapace physiognomy, anterolateral armature, male abdominal structure and sharply tapering male first pleopod. This species appears to be closely allied to the poorly known P. maindroni from Sumatra.
Tipo: Article / Letter to the editor Palavras-chave: Decapoda; Crustacea; Brachyura; Gecarcinucoidea; Freshwater crab; Parathelphusa; Blackwater swamps; Singapore; 42.74.
Ano: 1990 URL: http://www.repository.naturalis.nl/record/317928
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