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Registros recuperados: 13
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Capillary electrophoresis determination of the binding affinity of bioactive sulfated polysaccharides to proteins: study of the binding properties of fucoidan to antithrombin ArchiMer
Varenne, A; Gareil, P; Colliec-jouault, Sylvia; Daniel, R.
The interaction of proteins with polysaccharides represents a major and challenging topic in glycobiology, since such complexes mediate fundamental biological mechanisms. An affinity capillary electrophoresis method has been developed to evidence the complex formation and to determine the binding properties between an anticoagulant polysaccharide of marine origin, fucoidan, and a potential target protein, antithrombin. This method is a variant of zonal electrophoresis in the mobility shift format. A fixed amount of protein was injected into a capillary filled with a background electrolyte containing the polysaccharide in varying concentrations. The effective mobility data of the protein were processed according to classical linearization treatments to...
Tipo: Text Palavras-chave: Antithrombin; Fucoidan; Electrophoresis; Protein interaction; Polysaccharides.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-2114.pdf
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Effect of Low Molecular Weight Fucoidan and Low Molecular Weight Heparin in a Rabbit Model of Arterial Thrombosis ArchiMer
Durand, E; Helley, D; Zen, A; Dujols, C; Bruneval, P; Colliec-jouault, Sylvia; Fischer, Aurelie; Lafont, A.
Background: Therapeutic use of unfractionated heparin and low molecular weight heparins (LMWHs) is limited by hemorrhagic adverse effects. We compared the antithrombotic effect of LMW fucoidan (LMWF) and LMWH in an experimental model. Methods: Thrombosis was induced in femoral arteries of male New Zealand White rabbits by in situ induction of endothelial apoptosis with staurosporine (10(-5) M for 30 min). Starting the day before apoptosis induction, the animals received subcutaneous LMWF (15 mg/kg), LMWH (enoxaparin 2.5 mg/kg) or saline solution (control group) twice a day for 4 days. Results: The degrees of apoptosis and endothelial denudation were similar in the 3 groups. The thrombotic score was significantly lower in the LMWF group than in the LMWH and...
Tipo: Text Palavras-chave: Tissue factor; Tissue factor pathway inhibitor; Heparin; Fucoidan; Thrombosis.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4744.pdf
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Effect of low-molecular-weight fucoidan on experimental arterial thrombosis in the rabbit and rat ArchiMer
Colliec-jouault, Sylvia; Millet, J; Helley, D; Sinquin, Corinne; Fischer, Am.
Tipo: Text Palavras-chave: Arterial thrombosis; Polyaccharide; Fucoidan.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-2116.pdf
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Fucoidan a sulfated polysaccharide from brown algae is a potent modulator of connective tissue proteolysis ArchiMer
Senni, Karim; Gueniche, Farida; Foucault Bertaud, Alexandrine; Igondjo Tchen, Sylvie; Fioretti, Florence; Colliec-jouault, Sylvia; Durand, Patrick; Guezennec, Jean; Godeau, Gaston; Letourneur, Didier.
Fucoidans are sulfated fucosylated polymers from brown algae cell wall that exhibit some heparin/heparan sulfate properties. We previously demonstrated that these polysaccharides were able in vitro to stimulate dermal fibroblast proliferation and extracellular matrix deposition. Here, we investigated the action of a 16 kDa fucoidan fraction on parameters involved in connective tissue breakdown. This fucoidan is able to inhibit gelatinase A secretion and stromelysin 1 induction by interleukin-1 beta on dermal fibroblasts in culture. Furthermore, we observed that fucoidan increases the rate of association of MMPs with their specific inhibitors namely TIMPs. Using tissue sections of human skin in ex vivo experiments, we evidenced that this polysaccharide was...
Tipo: Text Palavras-chave: Interleukin 1 beta; Leukocyte elastase; Matrix metalloproteinases; Extracellular matrix remodeling; Glycosaminoglycans; Fucoidan.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2147.pdf
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FUCOIDAN INHIBITS THE IN VITRO GROWTH OF BABESIA BOVIS OAK
Takabatake, Noriyuki; Hashiba, Shintaro; Bork, Sabine; Okamura, Masashi; Yokoyama, Naoaki; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
Palavras-chave: Babesia bovis; Fucoidan.
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/646
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Fucoidan prevents concanavalin A-induced liver injury through induction of endogenous IL-10 in mice OAK
Saito, Ako; Yoneda, Masashi; Yokohama, Shiro; Okada, Mitsuyoshi; Haneda, Masakazu; Nakamura, Kimihide; 中村, 公英.
Fucoidan is a complex of sulfated polysaccharides derived from non-mammalian origin such as marine brown algae and induces cytokine expression. We investigated the effect of fucoidan on concanavalin A (Con A)-induced liver injury in mice. Liver injury was induced by an intravenous injection of Con A (18.5 mg/kg). Various doses of fucoidan (1-30 mg/kg) were intravenously administered 30 min before Con A injection. The plasma alanine aminotransferase (ALT) and several cytokines levels were determined, and hepatic histological changes were also assessed. The effect of fucoidan administration by itself on induction of interleukin (IL)-10 in plasma and liver tissue was investigated. Con A administration induced an elevation of plasma ALT level, and fucoidan...
Palavras-chave: Concanavalin A; Fucoidan; Liver injury; Cytokine; Kupffer cell; Scavenger receptor.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/66
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Heterofucans from Dictyota menstrualis have anticoagulant activity BJMBR
Albuquerque,I.R.L.; Queiroz,K.C.S.; Alves,L.G.; Santos,E.A.; Leite,E.L.; Rocha,H.A.O..
Fucan is a term used to denote a family of sulfated L-fucose-rich polysaccharides which are present in the extracellular matrix of brown seaweed and in the egg jelly coat of sea urchins. Plant fucans have several biological activities, including anticoagulant and antithrombotic, related to the structural and chemical composition of polysaccharides. We have extracted sulfated polysaccharides from the brown seaweed Dictyota menstrualis by proteolytic digestion, followed by separation into 5 fractions by sequential acetone precipitation. Gel electrophoresis using 0.05 M 1,3-diaminopropane-acetate buffer, pH 9.0, stained with 0.1% toluidine blue, showed the presence of sulfated polysaccharides in all fractions. The chemical analyses demonstrated that all...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Fucan; Fucoidan; Anticoagulant drugs; Brown algae; Antithrombotic agents.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200002
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Low molecular weight fucoidan promotes FGF-2-induced vascular tube formation by human endothelial cells, with decreased PAI-1 release and ICAM-1 downregulation ArchiMer
Chabut, D; Fischer, A; Helley, D; Colliec-jouault, Sylvia.
Tipo: Text Palavras-chave: Fucoidan; Vascularization; Endothelial cell; Human therapy.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-2115.pdf
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Low-molecular-weight fucoidan enhances the proangiogenic phenotype of endothelial progenitor cells ArchiMer
Zemani, Faouzia; Benisvy, Danielle; Galy Fauroux, Isabelle; Lokajczyk, Anna; Colliec-jouault, Sylvia; Uzan, Georges; Fischer, Anne Marie; Boisson Vidal, Catherine.
Endothelial progenitor cell (EPC) transplantation is a potential means of inducing neovascularization in vivo. However, the number of circulating EPC is relatively small, it may thus be necessary to enhance their proangiogenic properties ex vivo prior to injection in vivo. Fucoidan has previously been shown to potentiate in vitro tube formation by mature endothelial cells in the presence of basic fibroblast growth factor (FGF-2). We therefore examined whether fucoidan, alone or combined with FGF-2, could increase EPC proangiogenic potency in vitro. EPC exposure to 10 mu g/ml fucoidan induced a proangiogenic phenotype, including cell proliferation (p < 0.01) and migration (p < 0.01); moreover, differentiation into vascular cords occurred in the...
Tipo: Text Palavras-chave: Fucoidan; FGF 2; Endothelial progenitor cells; Angiogenesis; Alpha 6.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-679.pdf
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Low-Molecular-Weight Fucoidan Induces Endothelial Cell Migration via the PI3K/AKT Pathway and Modulates the Transcription of Genes Involved in Angiogenesis ArchiMer
Bouvard, Claire; Galy-fauroux, Isabelle; Grelac, Francoise; Carpentier, Wassila; Lokajczyk, Anna; Gandrille, Sophie; Colliec-jouault, Sylvia; Fischer, Anne-marie; Helley, Dominique.
Low-molecular-weight fucoidan (LMWF) is a sulfated polysaccharide extracted from brown seaweed that presents antithrombotic and pro-angiogenic properties. However, its mechanism of action is not well-characterized. Here, we studied the effects of LMWF on cell signaling and whole genome expression in human umbilical vein endothelial cells and endothelial colony forming cells. We observed that LMWF and vascular endothelial growth factor had synergistic effects on cell signaling, and more interestingly that LMWF by itself, in the absence of other growth factors, was able to trigger the activation of the PI3K/AKT pathway, which plays a crucial role in angiogenesis and vasculogenesis. We also observed that the effects of LMWF on cell migration were...
Tipo: Text Palavras-chave: Fucoidan; Angiogenesis; Vasculogenesis; Migration; Signaling; Transcriptomics.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00308/41913/41187.pdf
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Neoangiogenesis Induced by Progenitor Endothelial Cells: Effect of Fucoidan from Marine Algae ArchiMer
Boisson-vidal, C; Zemani, F; Calliguiri, G; Galy-fauroux, I; Colliec-jouault, Sylvia; Helley, D; Fischer, A-m.
Fucoidans -- sulphated polysaccharides extracted from brown algae - could be beneficial in patients with ischemic diseases. Their antithrombotic and proangiogenic properties promote in animals, neovascularization and angiogenesis which prevent necrosis of ischemic tissue. In 1997, endothelial progenitor cells were first identified in human peripheral blood. They are recruited from bone marrow and contribute to neovascularization after ischemic injury. Mobilization of these cells in ischemic sites is an important step in new vessel formation. It is thought that the progenitors interact with endothelial cells, then extravasate and reach ischemic sites, where they proliferate and differentiate into new blood vessels. Although chemokines, cytokines and...
Tipo: Text Palavras-chave: Growth factors; Cytokines; Angiogenesis; Neovascularization; Endothelial progenitor cells; Sulphated polysaccharides; Fucoidan.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2168.pdf
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Potential effects of a low molecular weight Fucoidan extracted from brown algae on bone biomaterial osteoconductive properties ArchiMer
Igondjo Tchen Changotade, S; Korb, G; Bassil, J; Barroukh, B; Willig, C; Colliec-jouault, Sylvia; Durand, Patrick; Godeau, G; Senni, K.
In this work we firstly tested the influence of low molecular weight fucoidan extracted from pheophicae cell wall on bidimensional cultured normal human osteoblasts behaviours. Secondly impregnation procedure with LMW fucoidan of bone biomaterial (Lubboc®) we explored in this bone extracellular matrix context its capabilities to support human osteoblastic behaviour in 3D culture. In bidimensionnal cultures we evidenced that: LMW fucoidan promotes human osteoblast proliferation, collagen type I expression and favours precocious alkaline phosphatase activity. Furthermore with LMW fucoidan von Kossa's staining was positive at 30 days and only positive at 45 days in absence of LMW fucoidan. In our three dimensional culture models with the biomaterial...
Tipo: Text Palavras-chave: Heparan mimetics; Biomaterial; Bone; Osteoblast; Fucoidan.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2007/publication-2695.pdf
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Therapeutic effect of fucoidan-stimulated endothelial colony-forming cells in peripheral ischemia ArchiMer
Sarlon, G.; Zemani, F.; David, L.; Van Huyen, J. -p. Duong; Dizier, B.; Grelac, F.; Colliec-jouault, Sylvia; Galy-fauroux, Isabelle; Bruneval, P.; Fischer, Anne-marie; Emmerich, J.; Boisson-vidal, Catherine.
. Background: Fucoidan, an antithrombotic polysaccharide, can induce endothelial colony-forming cells (ECFC) to adopt an angiogenic phenotype in vitro. Objectives: We evaluated the effect of fucoidan on vasculogenesis induced by ECFC in vivo. Methods: We used a murine hindlimb ischemia model to probe the synergic role of fucoidan-treatment and ECFC infusion during tissue repair. Results: We found that exposure of ECFC to fucoidan prior to their intravenous injection improved residual muscle blood flow and increased collateral vessel formation. Necrosis of ischemic tissue was significantly reduced on day 14, to 12.1% of the gastronecmius cross-sectional surface area compared with 40.1% in animals injected with untreated-ECFC. ECFC stimulation with fucoidan...
Tipo: Text Palavras-chave: Angiogenesis; Endothelial colony-forming cells; Fucoidan; Ischemia; Revascularization.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00065/17616/18204.pdf
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